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The map label for this gene is folC [H]

Identifier: 50083876

GI number: 50083876

Start: 632413

End: 633699

Strand: Direct

Name: folC [H]

Synonym: ACIAD0644

Alternate gene names: 50083876

Gene position: 632413-633699 (Clockwise)

Preceding gene: 50083875

Following gene: 50083877

Centisome position: 17.57

GC content: 42.97

Gene sequence:

>1287_bases
TTGAAACTCGACGCTCCAAGTATCCAAGATGATTTAATGACATGGCTCACATATTGGGGCCATGTTCACGTTACGGGGAT
TGATTTAGGTCTAGAACGTGTCATTCCCGTTGCTGAAAAACTCGGTGTCACCCAAACCTCGGCCAAAGTATTCACTGTGG
CTGGCACCAATGGTAAAGGTTCAACCACCACAACACTTGCTTCAATTTTGACTGAACAAGGCTATCGGGTGGGACTTTAT
CAGTCGCCTCATATTTACCGTTTTAATGAACGCGTTAAATTAAATGGTCAGGAAGCGTCAGATCAACAGCTCATTGAGGC
TTTTGTCAAAGTTGATCAGGTGCGTCGTGAATGTGGACTCAGTTTATCTTTTTTTGAAGCCACTACACTGGCCGCATTTC
TGATATTTCAGCAGCAGGCTTGTGATATTTGGGTATTGGAAGTTGGCTTAGGCGGACGTCTGGATGTTGTGAATGTGATT
GATCCCGATGTTGCAGTCATCACCAATATTGGTTTAGATCATACCGACTGGCTTGGCGATACCATCGAAAAAATTGCGTT
TGAAAAAGCAGGAATTATTCGTTCGAACATCCCCGTGATTTTTGGAGGAGTACAAACTTTACCTCAGGCGATTGCAGACA
AAGCAGCAGCGCAACAAGCACAACTTTATGTACTGGGGCGTGACTATTTTTATCAGGTTGCACCAGATCAGAAAAGCTGG
AATTTTGCCAGTTCGGGAACTACGCTTAAATTGCCACTGGGTTCGTTGGCACTTGAAAATATTTCCACGGCGGTCGCAGC
CTTATTGGTGAGTGGCATTGATGTTTCTCAAGCAGCCATCACCAGTGGAATCGAAAAAGCACATCTTGCAGGACGTTTTG
AACAGAGAATAATAAAAAATAAAGCGGTTGTTTTTGATGCTGGACACAATGGTCATGGTGTTGATTTTTTGTTAAAGCAA
TTGCGAAATTTCTTAGAATACAATAAACAGTACACAGAAGTGATCGCTGTCTTTTCAATGTTGGCAGATAAAGATATAAA
AGCGGTTGTCAATTTGCTGAAACCTGCTGTTTTACATTGGAAAGTTGCTGCATTAAGCGTTCCACGGGCTGCGCCAGTAA
CCCAATTACTTGATGCACTCGATGGCGAAATAACTCAGCAGTATGACAGTATTCGAGAGGCTTTCAGTGTTGCGCTTGAG
GAATCTCGACCTCATCAGCTGATTTTGGTCTGTGGATCGTTTCATACTTTAGAAGCGGTTTGGGAGTATTTAGAAAAATG
TCAATGA

Upstream 100 bases:

>100_bases
CCGAATATTTGCTTGATCACGGTGTGGTCGATCAAATTGTTCATCGTCATGCATTACGTGATACTGTATCTCGTATTGTG
TCTAAACTGATGAATTTACC

Downstream 100 bases:

>100_bases
ATAGCAAGCAACGCTGGATGGGTGGCGTTGTTTTATTAGGTGGTGGGGTTTTATTGGCAGCACTATTACTGAAAGGCAAA
GAAGAAATACATCAAACGCA

Product: bifunctional folylpolyglutamate synthase (FPGS)/dihydrofolate synthase

Products: NA

Alternate protein names: Folylpolyglutamate synthase; Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolylpolyglutamate synthase; Tetrahydrofolate synthase; Dihydrofolate synthase [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MKLDAPSIQDDLMTWLTYWGHVHVTGIDLGLERVIPVAEKLGVTQTSAKVFTVAGTNGKGSTTTTLASILTEQGYRVGLY
QSPHIYRFNERVKLNGQEASDQQLIEAFVKVDQVRRECGLSLSFFEATTLAAFLIFQQQACDIWVLEVGLGGRLDVVNVI
DPDVAVITNIGLDHTDWLGDTIEKIAFEKAGIIRSNIPVIFGGVQTLPQAIADKAAAQQAQLYVLGRDYFYQVAPDQKSW
NFASSGTTLKLPLGSLALENISTAVAALLVSGIDVSQAAITSGIEKAHLAGRFEQRIIKNKAVVFDAGHNGHGVDFLLKQ
LRNFLEYNKQYTEVIAVFSMLADKDIKAVVNLLKPAVLHWKVAALSVPRAAPVTQLLDALDGEITQQYDSIREAFSVALE
ESRPHQLILVCGSFHTLEAVWEYLEKCQ

Sequences:

>Translated_428_residues
MKLDAPSIQDDLMTWLTYWGHVHVTGIDLGLERVIPVAEKLGVTQTSAKVFTVAGTNGKGSTTTTLASILTEQGYRVGLY
QSPHIYRFNERVKLNGQEASDQQLIEAFVKVDQVRRECGLSLSFFEATTLAAFLIFQQQACDIWVLEVGLGGRLDVVNVI
DPDVAVITNIGLDHTDWLGDTIEKIAFEKAGIIRSNIPVIFGGVQTLPQAIADKAAAQQAQLYVLGRDYFYQVAPDQKSW
NFASSGTTLKLPLGSLALENISTAVAALLVSGIDVSQAAITSGIEKAHLAGRFEQRIIKNKAVVFDAGHNGHGVDFLLKQ
LRNFLEYNKQYTEVIAVFSMLADKDIKAVVNLLKPAVLHWKVAALSVPRAAPVTQLLDALDGEITQQYDSIREAFSVALE
ESRPHQLILVCGSFHTLEAVWEYLEKCQ
>Mature_428_residues
MKLDAPSIQDDLMTWLTYWGHVHVTGIDLGLERVIPVAEKLGVTQTSAKVFTVAGTNGKGSTTTTLASILTEQGYRVGLY
QSPHIYRFNERVKLNGQEASDQQLIEAFVKVDQVRRECGLSLSFFEATTLAAFLIFQQQACDIWVLEVGLGGRLDVVNVI
DPDVAVITNIGLDHTDWLGDTIEKIAFEKAGIIRSNIPVIFGGVQTLPQAIADKAAAQQAQLYVLGRDYFYQVAPDQKSW
NFASSGTTLKLPLGSLALENISTAVAALLVSGIDVSQAAITSGIEKAHLAGRFEQRIIKNKAVVFDAGHNGHGVDFLLKQ
LRNFLEYNKQYTEVIAVFSMLADKDIKAVVNLLKPAVLHWKVAALSVPRAAPVTQLLDALDGEITQQYDSIREAFSVALE
ESRPHQLILVCGSFHTLEAVWEYLEKCQ

Specific function: Conversion of folates to polyglutamate derivatives [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=158, Percent_Identity=37.3417721518987, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI66932990, Length=158, Percent_Identity=37.3417721518987, Blast_Score=116, Evalue=3e-26,
Organism=Escherichia coli, GI1788654, Length=421, Percent_Identity=42.9928741092637, Blast_Score=311, Evalue=5e-86,
Organism=Caenorhabditis elegans, GI17553148, Length=329, Percent_Identity=27.9635258358663, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71984923, Length=329, Percent_Identity=27.9635258358663, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17553150, Length=329, Percent_Identity=27.9635258358663, Blast_Score=123, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6323760, Length=330, Percent_Identity=31.5151515151515, Blast_Score=143, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6324815, Length=176, Percent_Identity=38.6363636363636, Blast_Score=111, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24641571, Length=190, Percent_Identity=37.3684210526316, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24581568, Length=200, Percent_Identity=31.5, Blast_Score=96, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17; =6.3.2.12 [H]

Molecular weight: Translated: 46967; Mature: 46967

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLDAPSIQDDLMTWLTYWGHVHVTGIDLGLERVIPVAEKLGVTQTSAKVFTVAGTNGKG
CCCCCCCHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCC
STTTTLASILTEQGYRVGLYQSPHIYRFNERVKLNGQEASDQQLIEAFVKVDQVRRECGL
CHHHHHHHHHHHCCCEEEEECCCCEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHHCC
SLSFFEATTLAAFLIFQQQACDIWVLEVGLGGRLDVVNVIDPDVAVITNIGLDHTDWLGD
EEEHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEECCCCEEEEEECCCCCCHHHHH
TIEKIAFEKAGIIRSNIPVIFGGVQTLPQAIADKAAAQQAQLYVLGRDYFYQVAPDQKSW
HHHHHHHHHCCCEECCCCEEECCHHHHHHHHHHHHHHCCEEEEEEECCEEEEECCCCCCC
NFASSGTTLKLPLGSLALENISTAVAALLVSGIDVSQAAITSGIEKAHLAGRFEQRIIKN
CCCCCCCEEEECHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHHHC
KAVVFDAGHNGHGVDFLLKQLRNFLEYNKQYTEVIAVFSMLADKDIKAVVNLLKPAVLHW
CEEEEECCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHE
KVAALSVPRAAPVTQLLDALDGEITQQYDSIREAFSVALEESRPHQLILVCGSFHTLEAV
EHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHH
WEYLEKCQ
HHHHHHCC
>Mature Secondary Structure
MKLDAPSIQDDLMTWLTYWGHVHVTGIDLGLERVIPVAEKLGVTQTSAKVFTVAGTNGKG
CCCCCCCHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCC
STTTTLASILTEQGYRVGLYQSPHIYRFNERVKLNGQEASDQQLIEAFVKVDQVRRECGL
CHHHHHHHHHHHCCCEEEEECCCCEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHHCC
SLSFFEATTLAAFLIFQQQACDIWVLEVGLGGRLDVVNVIDPDVAVITNIGLDHTDWLGD
EEEHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEECCCCEEEEEECCCCCCHHHHH
TIEKIAFEKAGIIRSNIPVIFGGVQTLPQAIADKAAAQQAQLYVLGRDYFYQVAPDQKSW
HHHHHHHHHCCCEECCCCEEECCHHHHHHHHHHHHHHCCEEEEEEECCEEEEECCCCCCC
NFASSGTTLKLPLGSLALENISTAVAALLVSGIDVSQAAITSGIEKAHLAGRFEQRIIKN
CCCCCCCEEEECHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHHHC
KAVVFDAGHNGHGVDFLLKQLRNFLEYNKQYTEVIAVFSMLADKDIKAVVNLLKPAVLHW
CEEEEECCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHE
KVAALSVPRAAPVTQLLDALDGEITQQYDSIREAFSVALEESRPHQLILVCGSFHTLEAV
EHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHH
WEYLEKCQ
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3040739; 3040734; 9205837; 9278503; 1989505 [H]