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The map label for this gene is ack [H]

Identifier: 50083779

GI number: 50083779

Start: 529730

End: 530935

Strand: Reverse

Name: ack [H]

Synonym: ACIAD0541

Alternate gene names: 50083779

Gene position: 530935-529730 (Counterclockwise)

Preceding gene: 50083785

Following gene: 50083778

Centisome position: 14.75

GC content: 44.2

Gene sequence:

>1206_bases
GTGTCTACATCAGTATTAGTTATTAACTGCGGTTCTTCATCAATCAAATATGCCTTGGTTTCAGAACGCCGTGAAGATCG
GATCTATGGATTGGCAGAAAATCTGGGTTCTGCCGATGCGCGCATCAAAGGAATTACCGTTGGCGGTGCACCATTAGAGC
TTTCCATCCCGTATGCTGACCATGAAAAAGCATTAGAAACCCTGCTAGGTCGCCTTGCAAACTACAAGCCACAGGCTATT
GGACACCGTGTAGTCCATGGCGGTACAAGCACCAAGGCAGAATTGCTTACACCAGAAATCATTGAAAAAATTCGTGAAGC
AACACCACTTGCACCTTTGCACAATCCTGCTCACCTTGTTGGAATTGATGCAACTATGCGTTTATTTCCAGAGTTACCAC
AAGTGGCAGTGTTTGATACAGCTTTTCATCAAACCATGCCAGCGCATGCATACCGTTATGCACTGCCTAAATTTTTATAT
ACCGAACACAATGTCCGTCGCTATGGCTTCCATGGAACCAGTCATGCCTATGTTTCTGAACGTGGAAGTGAACTGGCTGG
CAGTTTCAAACAAGGTGGCTGGCTCACTGCGCATTTAGGTAATGGCAGCTCAACTTGTGCTATCTGGAATGGTGAAAGTG
TTGATACCTCGATGGGTCTAACTCCGCTTGAAGGTATTGTGATGGGCACGCGTAGCGGCGATGTCGACCCAAGTATCCAT
AGCTTTTTGGCCAGTAATCTTGGATGGGATATTTTTAAAATCGATAAAATGCTGAATAAGGAAAGCGGCTTGCTCGGACT
TTCTGACAATTTATCTAATGATATGCGTACTCTAATTGAAGCCTCAGAACAAGGTAATGAAGACGCGACTCTTGCAATTG
AAGTATTTTGCTATCGTTTAGCCAAATCACTTGCTGCACTCAGCTGTGCACTACCTCGTCTGGATGGTTTATTCTTTACA
GGGGGTATTGGTGAAAACTCCGCCTACATTCGCGAAAAAACAATGGCATACTTGCCACACTTTGGTTTTAAACTAAACAT
AGAACAAAACAATACACTTAAGCGTGGTACAGAAGGTCGAATTGACCAAGGTACAGGTCCTCAGATTTGGGTTATTCCAA
CAGATGAGGAAGGCCGTATTGCCAAAGAAACCGAACAGGTGGTTGAACACGCAAAAAAGTCTGAATCAATGGTTAGCTTT
ACCTAG

Upstream 100 bases:

>100_bases
ACACGTGCTAAAACATCAACTATGATCTAACGTCTTAATCATTCATGGACTTGAAGTTTAAGTTAGCAAATAATCTACAA
CAGCATTCGGGTAAAATAAC

Downstream 100 bases:

>100_bases
CCATTGATCTTCAAACCAACATTGATTATTTGATAAGGCTTTTATGAATACAATTTTAATGATCCCGACTGGCGAAGGTG
TCGGGTTAACTTCAGCCTGT

Product: acetate kinase (propionate kinase)

Products: NA

Alternate protein names: Acetokinase [H]

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MSTSVLVINCGSSSIKYALVSERREDRIYGLAENLGSADARIKGITVGGAPLELSIPYADHEKALETLLGRLANYKPQAI
GHRVVHGGTSTKAELLTPEIIEKIREATPLAPLHNPAHLVGIDATMRLFPELPQVAVFDTAFHQTMPAHAYRYALPKFLY
TEHNVRRYGFHGTSHAYVSERGSELAGSFKQGGWLTAHLGNGSSTCAIWNGESVDTSMGLTPLEGIVMGTRSGDVDPSIH
SFLASNLGWDIFKIDKMLNKESGLLGLSDNLSNDMRTLIEASEQGNEDATLAIEVFCYRLAKSLAALSCALPRLDGLFFT
GGIGENSAYIREKTMAYLPHFGFKLNIEQNNTLKRGTEGRIDQGTGPQIWVIPTDEEGRIAKETEQVVEHAKKSESMVSF
T

Sequences:

>Translated_401_residues
MSTSVLVINCGSSSIKYALVSERREDRIYGLAENLGSADARIKGITVGGAPLELSIPYADHEKALETLLGRLANYKPQAI
GHRVVHGGTSTKAELLTPEIIEKIREATPLAPLHNPAHLVGIDATMRLFPELPQVAVFDTAFHQTMPAHAYRYALPKFLY
TEHNVRRYGFHGTSHAYVSERGSELAGSFKQGGWLTAHLGNGSSTCAIWNGESVDTSMGLTPLEGIVMGTRSGDVDPSIH
SFLASNLGWDIFKIDKMLNKESGLLGLSDNLSNDMRTLIEASEQGNEDATLAIEVFCYRLAKSLAALSCALPRLDGLFFT
GGIGENSAYIREKTMAYLPHFGFKLNIEQNNTLKRGTEGRIDQGTGPQIWVIPTDEEGRIAKETEQVVEHAKKSESMVSF
T
>Mature_400_residues
STSVLVINCGSSSIKYALVSERREDRIYGLAENLGSADARIKGITVGGAPLELSIPYADHEKALETLLGRLANYKPQAIG
HRVVHGGTSTKAELLTPEIIEKIREATPLAPLHNPAHLVGIDATMRLFPELPQVAVFDTAFHQTMPAHAYRYALPKFLYT
EHNVRRYGFHGTSHAYVSERGSELAGSFKQGGWLTAHLGNGSSTCAIWNGESVDTSMGLTPLEGIVMGTRSGDVDPSIHS
FLASNLGWDIFKIDKMLNKESGLLGLSDNLSNDMRTLIEASEQGNEDATLAIEVFCYRLAKSLAALSCALPRLDGLFFTG
GIGENSAYIREKTMAYLPHFGFKLNIEQNNTLKRGTEGRIDQGTGPQIWVIPTDEEGRIAKETEQVVEHAKKSESMVSFT

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI1788633, Length=399, Percent_Identity=45.8646616541353, Blast_Score=321, Evalue=5e-89,
Organism=Escherichia coli, GI145693184, Length=385, Percent_Identity=47.2727272727273, Blast_Score=317, Evalue=9e-88,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR004372 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.1 [H]

Molecular weight: Translated: 43750; Mature: 43619

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS01075 ACETATE_KINASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTSVLVINCGSSSIKYALVSERREDRIYGLAENLGSADARIKGITVGGAPLELSIPYAD
CCCCEEEEECCCCCEEEHHHHCCHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEECCCCC
HEKALETLLGRLANYKPQAIGHRVVHGGTSTKAELLTPEIIEKIREATPLAPLHNPAHLV
HHHHHHHHHHHHHCCCCHHHCCEEEECCCCCCHHHCCHHHHHHHHHCCCCCCCCCCCEEE
GIDATMRLFPELPQVAVFDTAFHQTMPAHAYRYALPKFLYTEHNVRRYGFHGTSHAYVSE
ECCHHHHHCCCCCCEEEEHHHHHHCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHH
RGSELAGSFKQGGWLTAHLGNGSSTCAIWNGESVDTSMGLTPLEGIVMGTRSGDVDPSIH
CCHHHHCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCCCCCCEEEECCCCCCCHHHH
SFLASNLGWDIFKIDKMLNKESGLLGLSDNLSNDMRTLIEASEQGNEDATLAIEVFCYRL
HHHHHHCCCHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCEEHHHHHHHHH
AKSLAALSCALPRLDGLFFTGGIGENSAYIREKTMAYLPHFGFKLNIEQNNTLKRGTEGR
HHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCC
IDQGTGPQIWVIPTDEEGRIAKETEQVVEHAKKSESMVSFT
CCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEECC
>Mature Secondary Structure 
STSVLVINCGSSSIKYALVSERREDRIYGLAENLGSADARIKGITVGGAPLELSIPYAD
CCCEEEEECCCCCEEEHHHHCCHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEECCCCC
HEKALETLLGRLANYKPQAIGHRVVHGGTSTKAELLTPEIIEKIREATPLAPLHNPAHLV
HHHHHHHHHHHHHCCCCHHHCCEEEECCCCCCHHHCCHHHHHHHHHCCCCCCCCCCCEEE
GIDATMRLFPELPQVAVFDTAFHQTMPAHAYRYALPKFLYTEHNVRRYGFHGTSHAYVSE
ECCHHHHHCCCCCCEEEEHHHHHHCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHH
RGSELAGSFKQGGWLTAHLGNGSSTCAIWNGESVDTSMGLTPLEGIVMGTRSGDVDPSIH
CCHHHHCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCCCCCCEEEECCCCCCCHHHH
SFLASNLGWDIFKIDKMLNKESGLLGLSDNLSNDMRTLIEASEQGNEDATLAIEVFCYRL
HHHHHHCCCHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCEEHHHHHHHHH
AKSLAALSCALPRLDGLFFTGGIGENSAYIREKTMAYLPHFGFKLNIEQNNTLKRGTEGR
HHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCC
IDQGTGPQIWVIPTDEEGRIAKETEQVVEHAKKSESMVSFT
CCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA