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The map label for this gene is leuA [H]

Identifier: 50083768

GI number: 50083768

Start: 514557

End: 516254

Strand: Reverse

Name: leuA [H]

Synonym: ACIAD0530

Alternate gene names: 50083768

Gene position: 516254-514557 (Counterclockwise)

Preceding gene: 50083769

Following gene: 50083767

Centisome position: 14.35

GC content: 41.99

Gene sequence:

>1698_bases
ATGATGTTGGCAGATCCAAGCAAAAAATACCGTCGCATGTACCAGCGAGTCGATTTACCAGACCGCCAGTGGCCAAATAA
TGAAATTACCAAAGCGCCCATCTGGATGAGTACCGATTTACGTGATGGTAACCAAGCTATTTTTGAACCGATGGATATCG
ATCAAAAATTTAAAATGTTTCAAATGTTGGTCAAAATTGGCTTTAAACATATTGAAATTGGTTTTCCATCTGCATCACAG
ATTGATTTTGACTTTACCCGCAAATTAATTGAAGAAAACCATATTCCAGATGATGTGTATATCGAAGTATTGGTACAGGC
ACGCGATCATTTAATTGAGCGTACTTTTGAATCTTTAGCTGGTGCTAAACGCGCGATCGTGCATATTTATAATGCCAACT
CTCCAACTTTCCGCCAGAAAGTTTTAAATGTCGGCGTTGAGGGTGCCAAAAAATTGGCCATCAATGCTGCTCATAAAGTC
AAGGAATATGCAGCACAGTATCCGCAAACTGACTGGATTTTTCAGTACAGTCCAGAATGCTTCTCTGCCACTGAATTAGA
AGTGGCAAAAGATGTCTGTGACGCTGTGACTGAAATCTGGGAAGCATCACCAGAAAATAAAGTAATCTTAAACTTGCCAG
CGACTGTCGAAGTGTCAACACCGAATGTCTATGCCGATCAAATTGAGTGGATGCACCGTAATCTTGCTCGTCGTGATGGC
GTGATTATTTCTGTTCACTGCCATAATGACCGTGGCTGTGGGATTGCAGCATCTGAGCTTGCCATTATGGCGGGTGCAGA
CCGTGTTGAAGGCTGTGTGTTTGGAAACGGCGAACGTACAGGTAATGTCGACGTTGCTGCAATTGCTTTGAATATGTATA
CCCAAGGTGTGGCACCAGAGTTAGATTTTTCTAATATCAATGAAATTATTGCGACTGTTGAAGAATGTACAGGCTTACCT
GTACATCCGCGTCATCCATATGCGGGTGATTTGGTCTTCACCGCATTCTCTGGTTCACATCAGGATGCCATTAAGAAAGG
CTTTGAATTCCAGAAAAATGAAGACATCTGGGATATGCCGTACTTACCAATCGATCCAAAAGATTTGGGGCGTGACTACG
ATGCGGTGATTCGTGTCAACAGCCAATCGGGTAAAGGTGGCATTGCGTACTTGTTAGAATCGAACTACAACGTGGTGTTA
CCGCGTCGTTTACAGATTGAGTTTTCACAAATCGTTCAACAAACCACAGATGCCAATGGTACAGAAATCAGTGCCTCTGA
AATCTGGAAATTATTCCAAGATACCTATGTGCAAGCAAAAGAACAGCACTATACTGCGAAAAACTATCGTTTATCTGATA
TCAATGGAACCCAAATTATTGAATTAGATATTGATATTGATGGTGAAATTCAACAGCTTCGCGGTGAAGGTAATGGACCA
ATTTCCGCATTTATGAATGCACTTCAATTACCAATTGATGTATTAAACTATGAAGAACGCAGCATCAGCTCAGGTGCTAA
TGCCAAAGCCTTAACACTGATTGAAATTCAAGTAAAAGGTACTGGCAAGAGTGCATTTGGTGCAGGCGTACATGACAATA
CCGTCACTTCATCTATTGAAGCGATCCTTGCATGTACCAACCGCTTGATTGAGCAAGGTGTACTCAGCACAGAACAAGTT
GCTGCTGCCGCAGTTTAA

Upstream 100 bases:

>100_bases
CTTGTTTTTTATTTAAATTTTAGTCATTCAATACCATATATAAATGTAGTGCTTTGCCTGAGAACAATCCCTCAAGCGCG
CTACGACAAGGAGCACTCTC

Downstream 100 bases:

>100_bases
TCTCGGTAAAAGACTCTTTTTGAAAGGATACCTATGGTATCCTTTCTTTTATTATTGATTTAAAGATAAAAGGCGAATTG
CTCCCGTGTCTTTTCCATCT

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 565; Mature: 565

Protein sequence:

>565_residues
MMLADPSKKYRRMYQRVDLPDRQWPNNEITKAPIWMSTDLRDGNQAIFEPMDIDQKFKMFQMLVKIGFKHIEIGFPSASQ
IDFDFTRKLIEENHIPDDVYIEVLVQARDHLIERTFESLAGAKRAIVHIYNANSPTFRQKVLNVGVEGAKKLAINAAHKV
KEYAAQYPQTDWIFQYSPECFSATELEVAKDVCDAVTEIWEASPENKVILNLPATVEVSTPNVYADQIEWMHRNLARRDG
VIISVHCHNDRGCGIAASELAIMAGADRVEGCVFGNGERTGNVDVAAIALNMYTQGVAPELDFSNINEIIATVEECTGLP
VHPRHPYAGDLVFTAFSGSHQDAIKKGFEFQKNEDIWDMPYLPIDPKDLGRDYDAVIRVNSQSGKGGIAYLLESNYNVVL
PRRLQIEFSQIVQQTTDANGTEISASEIWKLFQDTYVQAKEQHYTAKNYRLSDINGTQIIELDIDIDGEIQQLRGEGNGP
ISAFMNALQLPIDVLNYEERSISSGANAKALTLIEIQVKGTGKSAFGAGVHDNTVTSSIEAILACTNRLIEQGVLSTEQV
AAAAV

Sequences:

>Translated_565_residues
MMLADPSKKYRRMYQRVDLPDRQWPNNEITKAPIWMSTDLRDGNQAIFEPMDIDQKFKMFQMLVKIGFKHIEIGFPSASQ
IDFDFTRKLIEENHIPDDVYIEVLVQARDHLIERTFESLAGAKRAIVHIYNANSPTFRQKVLNVGVEGAKKLAINAAHKV
KEYAAQYPQTDWIFQYSPECFSATELEVAKDVCDAVTEIWEASPENKVILNLPATVEVSTPNVYADQIEWMHRNLARRDG
VIISVHCHNDRGCGIAASELAIMAGADRVEGCVFGNGERTGNVDVAAIALNMYTQGVAPELDFSNINEIIATVEECTGLP
VHPRHPYAGDLVFTAFSGSHQDAIKKGFEFQKNEDIWDMPYLPIDPKDLGRDYDAVIRVNSQSGKGGIAYLLESNYNVVL
PRRLQIEFSQIVQQTTDANGTEISASEIWKLFQDTYVQAKEQHYTAKNYRLSDINGTQIIELDIDIDGEIQQLRGEGNGP
ISAFMNALQLPIDVLNYEERSISSGANAKALTLIEIQVKGTGKSAFGAGVHDNTVTSSIEAILACTNRLIEQGVLSTEQV
AAAAV
>Mature_565_residues
MMLADPSKKYRRMYQRVDLPDRQWPNNEITKAPIWMSTDLRDGNQAIFEPMDIDQKFKMFQMLVKIGFKHIEIGFPSASQ
IDFDFTRKLIEENHIPDDVYIEVLVQARDHLIERTFESLAGAKRAIVHIYNANSPTFRQKVLNVGVEGAKKLAINAAHKV
KEYAAQYPQTDWIFQYSPECFSATELEVAKDVCDAVTEIWEASPENKVILNLPATVEVSTPNVYADQIEWMHRNLARRDG
VIISVHCHNDRGCGIAASELAIMAGADRVEGCVFGNGERTGNVDVAAIALNMYTQGVAPELDFSNINEIIATVEECTGLP
VHPRHPYAGDLVFTAFSGSHQDAIKKGFEFQKNEDIWDMPYLPIDPKDLGRDYDAVIRVNSQSGKGGIAYLLESNYNVVL
PRRLQIEFSQIVQQTTDANGTEISASEIWKLFQDTYVQAKEQHYTAKNYRLSDINGTQIIELDIDIDGEIQQLRGEGNGP
ISAFMNALQLPIDVLNYEERSISSGANAKALTLIEIQVKGTGKSAFGAGVHDNTVTSSIEAILACTNRLIEQGVLSTEQV
AAAAV

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=537, Percent_Identity=23.0912476722533, Blast_Score=105, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6324225, Length=580, Percent_Identity=48.9655172413793, Blast_Score=566, Evalue=1e-162,
Organism=Saccharomyces cerevisiae, GI6324682, Length=572, Percent_Identity=48.951048951049, Blast_Score=558, Evalue=1e-159,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005668
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 62949; Mature: 62949

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMLADPSKKYRRMYQRVDLPDRQWPNNEITKAPIWMSTDLRDGNQAIFEPMDIDQKFKMF
CCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHCCCCCCHHHHHHH
QMLVKIGFKHIEIGFPSASQIDFDFTRKLIEENHIPDDVYIEVLVQARDHLIERTFESLA
HHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHC
GAKRAIVHIYNANSPTFRQKVLNVGVEGAKKLAINAAHKVKEYAAQYPQTDWIFQYSPEC
CCCEEEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCC
FSATELEVAKDVCDAVTEIWEASPENKVILNLPATVEVSTPNVYADQIEWMHRNLARRDG
CCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEECCCCCHHHHHHHHHHHHHHHCC
VIISVHCHNDRGCGIAASELAIMAGADRVEGCVFGNGERTGNVDVAAIALNMYTQGVAPE
EEEEEEECCCCCCCEEHHHHHEEECCCHHCEEEECCCCCCCCEEEEEEEEEHHHCCCCCC
LDFSNINEIIATVEECTGLPVHPRHPYAGDLVFTAFSGSHQDAIKKGFEFQKNEDIWDMP
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCHHHHHCCCCCCCCCCCCCCC
YLPIDPKDLGRDYDAVIRVNSQSGKGGIAYLLESNYNVVLPRRLQIEFSQIVQQTTDANG
CCCCCHHHHCCCEEEEEEEECCCCCCCEEEEEECCCCEEECEEEEHHHHHHHHHHCCCCC
TEISASEIWKLFQDTYVQAKEQHYTAKNYRLSDINGTQIIELDIDIDGEIQQLRGEGNGP
CEECHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEEEEECCCCHHHHHCCCCCCC
ISAFMNALQLPIDVLNYEERSISSGANAKALTLIEIQVKGTGKSAFGAGVHDNTVTSSIE
HHHHHHHHHCCHHHHCCCHHHHCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCHHHHHHH
AILACTNRLIEQGVLSTEQVAAAAV
HHHHHHHHHHHHCCCCHHHHHHHCC
>Mature Secondary Structure
MMLADPSKKYRRMYQRVDLPDRQWPNNEITKAPIWMSTDLRDGNQAIFEPMDIDQKFKMF
CCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHCCCCCCHHHHHHH
QMLVKIGFKHIEIGFPSASQIDFDFTRKLIEENHIPDDVYIEVLVQARDHLIERTFESLA
HHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHC
GAKRAIVHIYNANSPTFRQKVLNVGVEGAKKLAINAAHKVKEYAAQYPQTDWIFQYSPEC
CCCEEEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCC
FSATELEVAKDVCDAVTEIWEASPENKVILNLPATVEVSTPNVYADQIEWMHRNLARRDG
CCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEECCCCCHHHHHHHHHHHHHHHCC
VIISVHCHNDRGCGIAASELAIMAGADRVEGCVFGNGERTGNVDVAAIALNMYTQGVAPE
EEEEEEECCCCCCCEEHHHHHEEECCCHHCEEEECCCCCCCCEEEEEEEEEHHHCCCCCC
LDFSNINEIIATVEECTGLPVHPRHPYAGDLVFTAFSGSHQDAIKKGFEFQKNEDIWDMP
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCHHHHHCCCCCCCCCCCCCCC
YLPIDPKDLGRDYDAVIRVNSQSGKGGIAYLLESNYNVVLPRRLQIEFSQIVQQTTDANG
CCCCCHHHHCCCEEEEEEEECCCCCCCEEEEEECCCCEEECEEEEHHHHHHHHHHCCCCC
TEISASEIWKLFQDTYVQAKEQHYTAKNYRLSDINGTQIIELDIDIDGEIQQLRGEGNGP
CEECHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEEEEECCCCHHHHHCCCCCCC
ISAFMNALQLPIDVLNYEERSISSGANAKALTLIEIQVKGTGKSAFGAGVHDNTVTSSIE
HHHHHHHHHCCHHHHCCCHHHHCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCHHHHHHH
AILACTNRLIEQGVLSTEQVAAAAV
HHHHHHHHHHHHCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA