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The map label for this gene is purU1 [H]

Identifier: 50083744

GI number: 50083744

Start: 496943

End: 497833

Strand: Direct

Name: purU1 [H]

Synonym: ACIAD0505

Alternate gene names: 50083744

Gene position: 496943-497833 (Clockwise)

Preceding gene: 50083743

Following gene: 50083745

Centisome position: 13.81

GC content: 42.31

Gene sequence:

>891_bases
CTGACCTATAGTGATATTAATCAAATGAACACCACTACTGCCAATACAGCACGTCTACTGATTACTTGTGAAGATAGGCC
CGGGATCGTGCAAGCTGTATCGAGCTTTTTGTATCATCAGGGAGCAAACATTACCGCACTTGATCAATATGCTACAGAAG
CTCAAGGTGGCCGTTATTTCATGCGTGTTGAGTTTGAGTTAGAGCATTTACAAAGTCGTAAAGATACATTGATCCAGACT
TTCTCTGCGAATGTTGCTGAGCGTTATAACATGCAATGGCGTTTGGCTTTTGTCAATGAGTTGAAGAAAGTGGGGATTTT
AGTTTCTAAAGTCGATCATGCTCTTTTAGAGTTGCTATGGCGTCATTCGCGTGGTGGTTTACCATGTGAAATTACTAAAG
TTGTATCAAATCACGAAGACTTACGTGAAGCGGTAGAAAATTTTGGTATTCCGTTTGAAGTGGTTCCTGTCAATAAAGAG
AACAAACGTGAAGCTTACGCCCAAATTGACGAATTGATGCAGGGCAATGACTTGTTAGTCCTTGCACGCTACATGCAAAT
TCTGGATGAAGCCTTCGTTGAGCGTTGGGAGATGAAAATTATCAATATTCATCATTCATTCCTACCTGCATTTGTGGGGG
CTAATCCGTATAAACAAGCCCATGAAAAAGGGGTGAAGCTAATTGGTGCAACTGCACACTACGTTACGGCCGATTTAGAT
CAGGGGCCGATCATTGAGCAGGACGTAGAACGAGTCAATCATGACTTTACCGTTGAACAGTTACGTGAACTGGGCCAAGA
TGTCGAGCGTAATGTGCTGGCACGTGCGGTGAAATGGCACCTTGAAGATCGAATTATTGTTGATGGCAATAAAACCGTTG
TTTTTCAATAA

Upstream 100 bases:

>100_bases
ATCTGAATTGTTTGCGAGTCTGTCGTTGAAAAGTGAGTAATTGATGAGCCCTATTCAGTCTGAGATAAAAACGCGCTAGA
ATAATCGGCTTGTTTTTTAA

Downstream 100 bases:

>100_bases
AATAAATCTCATTTACAGAAAAAGTATGTGGAGATGCATACTTTTTTTAGTCGAATAAAGTATTATGTTATAACATAATT
TATCTCAGATTAAATTGTTG

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MTYSDINQMNTTTANTARLLITCEDRPGIVQAVSSFLYHQGANITALDQYATEAQGGRYFMRVEFELEHLQSRKDTLIQT
FSANVAERYNMQWRLAFVNELKKVGILVSKVDHALLELLWRHSRGGLPCEITKVVSNHEDLREAVENFGIPFEVVPVNKE
NKREAYAQIDELMQGNDLLVLARYMQILDEAFVERWEMKIINIHHSFLPAFVGANPYKQAHEKGVKLIGATAHYVTADLD
QGPIIEQDVERVNHDFTVEQLRELGQDVERNVLARAVKWHLEDRIIVDGNKTVVFQ

Sequences:

>Translated_296_residues
MTYSDINQMNTTTANTARLLITCEDRPGIVQAVSSFLYHQGANITALDQYATEAQGGRYFMRVEFELEHLQSRKDTLIQT
FSANVAERYNMQWRLAFVNELKKVGILVSKVDHALLELLWRHSRGGLPCEITKVVSNHEDLREAVENFGIPFEVVPVNKE
NKREAYAQIDELMQGNDLLVLARYMQILDEAFVERWEMKIINIHHSFLPAFVGANPYKQAHEKGVKLIGATAHYVTADLD
QGPIIEQDVERVNHDFTVEQLRELGQDVERNVLARAVKWHLEDRIIVDGNKTVVFQ
>Mature_295_residues
TYSDINQMNTTTANTARLLITCEDRPGIVQAVSSFLYHQGANITALDQYATEAQGGRYFMRVEFELEHLQSRKDTLIQTF
SANVAERYNMQWRLAFVNELKKVGILVSKVDHALLELLWRHSRGGLPCEITKVVSNHEDLREAVENFGIPFEVVPVNKEN
KREAYAQIDELMQGNDLLVLARYMQILDEAFVERWEMKIINIHHSFLPAFVGANPYKQAHEKGVKLIGATAHYVTADLDQ
GPIIEQDVERVNHDFTVEQLRELGQDVERNVLARAVKWHLEDRIIVDGNKTVVFQ

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=209, Percent_Identity=30.622009569378, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI209869995, Length=209, Percent_Identity=30.622009569378, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI209869993, Length=209, Percent_Identity=30.622009569378, Blast_Score=90, Evalue=2e-18,
Organism=Escherichia coli, GI1787483, Length=284, Percent_Identity=41.5492957746479, Blast_Score=206, Evalue=1e-54,
Organism=Escherichia coli, GI1788846, Length=160, Percent_Identity=31.25, Blast_Score=72, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17567511, Length=181, Percent_Identity=32.0441988950276, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24582400, Length=203, Percent_Identity=32.0197044334975, Blast_Score=79, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF01842 ACT; PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 33948; Mature: 33817

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYSDINQMNTTTANTARLLITCEDRPGIVQAVSSFLYHQGANITALDQYATEAQGGRYF
CCCCHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCEEEHHHHHHHCCCCEEE
MRVEFELEHLQSRKDTLIQTFSANVAERYNMQWRLAFVNELKKVGILVSKVDHALLELLW
EEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
RHSRGGLPCEITKVVSNHEDLREAVENFGIPFEVVPVNKENKREAYAQIDELMQGNDLLV
HHCCCCCCCHHHHHHCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHCCCCHHH
LARYMQILDEAFVERWEMKIINIHHSFLPAFVGANPYKQAHEKGVKLIGATAHYVTADLD
HHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHCCCCHHHHHHCCCEEEECCHHEEEEECC
QGPIIEQDVERVNHDFTVEQLRELGQDVERNVLARAVKWHLEDRIIVDGNKTVVFQ
CCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEEEEC
>Mature Secondary Structure 
TYSDINQMNTTTANTARLLITCEDRPGIVQAVSSFLYHQGANITALDQYATEAQGGRYF
CCCHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCEEEHHHHHHHCCCCEEE
MRVEFELEHLQSRKDTLIQTFSANVAERYNMQWRLAFVNELKKVGILVSKVDHALLELLW
EEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
RHSRGGLPCEITKVVSNHEDLREAVENFGIPFEVVPVNKENKREAYAQIDELMQGNDLLV
HHCCCCCCCHHHHHHCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHCCCCHHH
LARYMQILDEAFVERWEMKIINIHHSFLPAFVGANPYKQAHEKGVKLIGATAHYVTADLD
HHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHCCCCHHHHHHCCCEEEECCHHEEEEECC
QGPIIEQDVERVNHDFTVEQLRELGQDVERNVLARAVKWHLEDRIIVDGNKTVVFQ
CCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]