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The map label for this gene is pcrA [H]

Identifier: 50083734

GI number: 50083734

Start: 488419

End: 490437

Strand: Direct

Name: pcrA [H]

Synonym: ACIAD0495

Alternate gene names: 50083734

Gene position: 488419-490437 (Clockwise)

Preceding gene: 50083733

Following gene: 50083735

Centisome position: 13.57

GC content: 43.54

Gene sequence:

>2019_bases
CTGCTACACTGGAGACTATCCCTTTTAGATGATTGTCGTTATTTACTTATGAGTTTTGAGCAGTTAATGGGTGAATTGAA
TCCCCAACAGCATCGTGCCGCAACCACGCAGGCCCAACATTGTCTGGTATTGGCTGGTGCAGGCTGTGGCAAAACTAAAA
CCATTATTGCACGTGCTGCCCATCTGATTCATCGTGGTACGCCAGCTGATCAGATTCAGATATTAACCTTTACCCGTCGC
GCAGCCAGCGAAATTGTGACCCGTGTGGAACAGCATTTGGGCGCACCTGCCAAAGGTTTACGTGCCTCAACCTTTCATAC
ATTTTGTATGTATCTGCTTAGACGTAACCCGCAAGCTTTTGGTTTGGGACAGTTCACAATTATCGATCGTGATGACCAAA
TGCTGATGTTTCGTCTGTTACGTGGTAAAGATAAAAATAATGTATTGCCAAAAGCTGCTCAGCTCTGTGATGTTTACTCT
TATGCACGCAACACACAATCCAAATTGTCAGATGCTTTGATCAAGCAATTACCTGAAGCACTTGAACATAAAGTCCAAAT
TGCAGAACTCATGCAAAGCTATGAGCAGCGCAAACAAGAACGTCATTTTCTTGATTACGATGATATTTTAGCGATTGTTG
CTGTCAATCTGCAAAATTCGCCTGAACTGGTGCAGTGGGTCACCAGATTCTGCAAAGTCATGTTGGTTGATGAAATGCAA
GATACCAACCCGTTGCAATGGGGGATTTTACAACCCTTGATTGAGCATGTTGGTCTATTTTGTGTTGGGGATGATGCTCA
ATCAATTTATGGCTTTCGAGGCGCAGATTTTGAAAATATCCATCATTTTAAAGAGCGCATTCCCAATGCGGAAGTACTGA
CACTTGAAGAAAACTACCGCTCCACCCAAGAGATTCTGGATATTTCTAACTGGTTATTGGCACATAGTCCAATTGAATAT
CACAAGCAGCTGAAAGCACATCGAGGACAAGGTTTAAAACCCCAACTGAATATTTTTCAGAATGAGTTTGAAGAAGCCAA
CTGGATTGCACAGGATTTACTGGCACGTCACCAGCAGGGTGCACACTGGCATGAGCATATGATTTTAGTCCGCTCAGGTT
TTTCGGCACGTTATCTGGAAGGGGCCTTAATTTCCTGTAACATTCCTTATCGATTTATTGGTGGCGTCAAGTTGCTTGAA
TCAGCGCATGTGAAAGATGTGCTCAGTATGCTGCGTGTCGTCATTAATCCTCAAGATGATATTGCATGGATGCGGTTTCT
CACGTTATGGAATGGCGTGGGTGATGTCGGTGCCAGCAAACTGGCGCATGAGCTGATTCAATTGCCAGATATTGATGCAC
GTTGTGAGCGTCTGGAACGTCACGATAAGGTGGATCCCAAAGCCATTCTCATTTTAAAGCAACTGGATGTATTACAGCAG
CATGTAGAGGCTTCAATTGGCTTGGCACTTGACGCATTGGCCGAACAATTAGAGCAAAACTATAAAACGCAAGACTGGTC
ACGACGGGTCAAGGATTTTGATCTGGTTAAACAGTTGGGGCGCAAGCATGTGGCATTGTCTGAGTTCTTAGAAGAATATG
TACTTGAACCAGTTTCAATTTCAGAGATTGAACGCTCTGGTGATCAGGACCTGGTCACCATCATTACCATTCATTCTGCA
AAAGGTGCCGAGCAAAAGGTATGTTATGTACCGCATGTTTCGCCAAATGCTTATCCTCATGCGCGAGCACAAGGCGATTT
TGATGAAGTAGAGGAAGAACGTCGGGTGCTATATGTGGCATTGACGCGTGCCGAGAATGAGCTAATTATTACCAAACAAA
ATTATAATACTTGGGCGCAGGATCAATATGATGAACAGGGCAGAAAAATCGAAAGCTACTTTTTAAATGATCTGCCTCAA
CACTTAGTAGATGCACATATCCATCAAACTGTGCCTAGATTTCCACAAAAGACACAAGTTCGGCAGAAACGATTTAGTTT
TGGGATTGATCTCGAGTAA

Upstream 100 bases:

>100_bases
AGCCTCCGCTATTAAACATCTGCCACACAGCTCTACCACTTCGACCTGATCATGATTCAATGTCGATTTAGGCATTGTTT
CATCTTTCAATGAATAGACT

Downstream 100 bases:

>100_bases
ACTGCCGCTAAGATGTAGGCTACTCTACATTGCCATTTATTTTGACATAAGGTTGTATATGAAAATTTTAGTTCGTAATC
TTGAGCGTTCTGTGACTGAA

Product: ATP-dependent DNA helicase (PcrA)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 672; Mature: 672

Protein sequence:

>672_residues
MLHWRLSLLDDCRYLLMSFEQLMGELNPQQHRAATTQAQHCLVLAGAGCGKTKTIIARAAHLIHRGTPADQIQILTFTRR
AASEIVTRVEQHLGAPAKGLRASTFHTFCMYLLRRNPQAFGLGQFTIIDRDDQMLMFRLLRGKDKNNVLPKAAQLCDVYS
YARNTQSKLSDALIKQLPEALEHKVQIAELMQSYEQRKQERHFLDYDDILAIVAVNLQNSPELVQWVTRFCKVMLVDEMQ
DTNPLQWGILQPLIEHVGLFCVGDDAQSIYGFRGADFENIHHFKERIPNAEVLTLEENYRSTQEILDISNWLLAHSPIEY
HKQLKAHRGQGLKPQLNIFQNEFEEANWIAQDLLARHQQGAHWHEHMILVRSGFSARYLEGALISCNIPYRFIGGVKLLE
SAHVKDVLSMLRVVINPQDDIAWMRFLTLWNGVGDVGASKLAHELIQLPDIDARCERLERHDKVDPKAILILKQLDVLQQ
HVEASIGLALDALAEQLEQNYKTQDWSRRVKDFDLVKQLGRKHVALSEFLEEYVLEPVSISEIERSGDQDLVTIITIHSA
KGAEQKVCYVPHVSPNAYPHARAQGDFDEVEEERRVLYVALTRAENELIITKQNYNTWAQDQYDEQGRKIESYFLNDLPQ
HLVDAHIHQTVPRFPQKTQVRQKRFSFGIDLE

Sequences:

>Translated_672_residues
MLHWRLSLLDDCRYLLMSFEQLMGELNPQQHRAATTQAQHCLVLAGAGCGKTKTIIARAAHLIHRGTPADQIQILTFTRR
AASEIVTRVEQHLGAPAKGLRASTFHTFCMYLLRRNPQAFGLGQFTIIDRDDQMLMFRLLRGKDKNNVLPKAAQLCDVYS
YARNTQSKLSDALIKQLPEALEHKVQIAELMQSYEQRKQERHFLDYDDILAIVAVNLQNSPELVQWVTRFCKVMLVDEMQ
DTNPLQWGILQPLIEHVGLFCVGDDAQSIYGFRGADFENIHHFKERIPNAEVLTLEENYRSTQEILDISNWLLAHSPIEY
HKQLKAHRGQGLKPQLNIFQNEFEEANWIAQDLLARHQQGAHWHEHMILVRSGFSARYLEGALISCNIPYRFIGGVKLLE
SAHVKDVLSMLRVVINPQDDIAWMRFLTLWNGVGDVGASKLAHELIQLPDIDARCERLERHDKVDPKAILILKQLDVLQQ
HVEASIGLALDALAEQLEQNYKTQDWSRRVKDFDLVKQLGRKHVALSEFLEEYVLEPVSISEIERSGDQDLVTIITIHSA
KGAEQKVCYVPHVSPNAYPHARAQGDFDEVEEERRVLYVALTRAENELIITKQNYNTWAQDQYDEQGRKIESYFLNDLPQ
HLVDAHIHQTVPRFPQKTQVRQKRFSFGIDLE
>Mature_672_residues
MLHWRLSLLDDCRYLLMSFEQLMGELNPQQHRAATTQAQHCLVLAGAGCGKTKTIIARAAHLIHRGTPADQIQILTFTRR
AASEIVTRVEQHLGAPAKGLRASTFHTFCMYLLRRNPQAFGLGQFTIIDRDDQMLMFRLLRGKDKNNVLPKAAQLCDVYS
YARNTQSKLSDALIKQLPEALEHKVQIAELMQSYEQRKQERHFLDYDDILAIVAVNLQNSPELVQWVTRFCKVMLVDEMQ
DTNPLQWGILQPLIEHVGLFCVGDDAQSIYGFRGADFENIHHFKERIPNAEVLTLEENYRSTQEILDISNWLLAHSPIEY
HKQLKAHRGQGLKPQLNIFQNEFEEANWIAQDLLARHQQGAHWHEHMILVRSGFSARYLEGALISCNIPYRFIGGVKLLE
SAHVKDVLSMLRVVINPQDDIAWMRFLTLWNGVGDVGASKLAHELIQLPDIDARCERLERHDKVDPKAILILKQLDVLQQ
HVEASIGLALDALAEQLEQNYKTQDWSRRVKDFDLVKQLGRKHVALSEFLEEYVLEPVSISEIERSGDQDLVTIITIHSA
KGAEQKVCYVPHVSPNAYPHARAQGDFDEVEEERRVLYVALTRAENELIITKQNYNTWAQDQYDEQGRKIESYFLNDLPQ
HLVDAHIHQTVPRFPQKTQVRQKRFSFGIDLE

Specific function: Essential helicase [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=670, Percent_Identity=29.8507462686567, Blast_Score=228, Evalue=1e-60,
Organism=Escherichia coli, GI48994965, Length=646, Percent_Identity=30.030959752322, Blast_Score=226, Evalue=4e-60,
Organism=Escherichia coli, GI1787196, Length=345, Percent_Identity=25.7971014492754, Blast_Score=96, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6322369, Length=477, Percent_Identity=28.9308176100629, Blast_Score=128, Evalue=3e-30,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 77320; Mature: 77320

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHWRLSLLDDCRYLLMSFEQLMGELNPQQHRAATTQAQHCLVLAGAGCGKTKTIIARAA
CCEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHH
HLIHRGTPADQIQILTFTRRAASEIVTRVEQHLGAPAKGLRASTFHTFCMYLLRRNPQAF
HHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCC
GLGQFTIIDRDDQMLMFRLLRGKDKNNVLPKAAQLCDVYSYARNTQSKLSDALIKQLPEA
CCCEEEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEHKVQIAELMQSYEQRKQERHFLDYDDILAIVAVNLQNSPELVQWVTRFCKVMLVDEMQ
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHCC
DTNPLQWGILQPLIEHVGLFCVGDDAQSIYGFRGADFENIHHFKERIPNAEVLTLEENYR
CCCCCHHHHHHHHHHHCCEEEECCCHHHHCCCCCCCHHHHHHHHHHCCCCEEEEEHHHHH
STQEILDISNWLLAHSPIEYHKQLKAHRGQGLKPQLNIFQNEFEEANWIAQDLLARHQQG
HHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
AHWHEHMILVRSGFSARYLEGALISCNIPYRFIGGVKLLESAHVKDVLSMLRVVINPQDD
CCHHHHEEEEECCCCHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
IAWMRFLTLWNGVGDVGASKLAHELIQLPDIDARCERLERHDKVDPKAILILKQLDVLQQ
HHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
HVEASIGLALDALAEQLEQNYKTQDWSRRVKDFDLVKQLGRKHVALSEFLEEYVLEPVSI
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
SEIERSGDQDLVTIITIHSAKGAEQKVCYVPHVSPNAYPHARAQGDFDEVEEERRVLYVA
HHHHCCCCCCEEEEEEEECCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHEEEEEE
LTRAENELIITKQNYNTWAQDQYDEQGRKIESYFLNDLPQHLVDAHIHQTVPRFPQKTQV
EEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
RQKRFSFGIDLE
HHHHHCCCCCCC
>Mature Secondary Structure
MLHWRLSLLDDCRYLLMSFEQLMGELNPQQHRAATTQAQHCLVLAGAGCGKTKTIIARAA
CCEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHH
HLIHRGTPADQIQILTFTRRAASEIVTRVEQHLGAPAKGLRASTFHTFCMYLLRRNPQAF
HHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCC
GLGQFTIIDRDDQMLMFRLLRGKDKNNVLPKAAQLCDVYSYARNTQSKLSDALIKQLPEA
CCCEEEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEHKVQIAELMQSYEQRKQERHFLDYDDILAIVAVNLQNSPELVQWVTRFCKVMLVDEMQ
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHCC
DTNPLQWGILQPLIEHVGLFCVGDDAQSIYGFRGADFENIHHFKERIPNAEVLTLEENYR
CCCCCHHHHHHHHHHHCCEEEECCCHHHHCCCCCCCHHHHHHHHHHCCCCEEEEEHHHHH
STQEILDISNWLLAHSPIEYHKQLKAHRGQGLKPQLNIFQNEFEEANWIAQDLLARHQQG
HHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
AHWHEHMILVRSGFSARYLEGALISCNIPYRFIGGVKLLESAHVKDVLSMLRVVINPQDD
CCHHHHEEEEECCCCHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
IAWMRFLTLWNGVGDVGASKLAHELIQLPDIDARCERLERHDKVDPKAILILKQLDVLQQ
HHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
HVEASIGLALDALAEQLEQNYKTQDWSRRVKDFDLVKQLGRKHVALSEFLEEYVLEPVSI
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
SEIERSGDQDLVTIITIHSAKGAEQKVCYVPHVSPNAYPHARAQGDFDEVEEERRVLYVA
HHHHCCCCCCEEEEEEEECCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHEEEEEE
LTRAENELIITKQNYNTWAQDQYDEQGRKIESYFLNDLPQHLVDAHIHQTVPRFPQKTQV
EEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
RQKRFSFGIDLE
HHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA