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The map label for this gene is ptsP [H]

Identifier: 50083702

GI number: 50083702

Start: 451233

End: 453527

Strand: Reverse

Name: ptsP [H]

Synonym: ACIAD0454

Alternate gene names: 50083702

Gene position: 453527-451233 (Counterclockwise)

Preceding gene: 50083703

Following gene: 50083700

Centisome position: 12.6

GC content: 44.92

Gene sequence:

>2295_bases
ATGTCAAACATGCAACTTGACACTTTGAGACGTATTGTTCAGGAGATCAATTCGTCCGTAAGTTTGCATGATTCGCTCGA
TATTATGGTCAATCAAGTGGCCGACGCGATGAAAGTGGATGTATGTTCGATTTATTTGCTTGATGAACGGAACCAGCGCT
ATTTACTGATGGCGTCAAAAGGACTCAATCCTGAGTCTGTCGGTCACGTCTCGTTACAGCTCAGCGAAGGTTTAGTCGGG
TTGGTCGGACAGCGTGAAGAAATCGTCAACTTGGAGAATGCCTCCAAGCACGAGCGCTTTGCTTATTTACCCGAAACTGG
AGAAGAAATTTACAATTCTTTTCTCGGTGTTCCTGTTATGTATCGCCGTAAGGTCATGGGCGTATTGGTGGTGCAAAACA
AACAGCCACAAGACTTTAGTGAGGCAGCCGAATCCTTTTTGGTGACCTTGTGCGCACAGCTTTCAGGTGTTATTGCCCAT
GCACATGCGGTAGGCAATATCGATGTTTTCCGTAAGCCAAGCAATGGTGCAACTTATAAAACCTTCCAGGGTGTACCTGG
TTCAGGTGGTATTGCATTAGGACGCGCAATTGTACTGTATCCTCCTGCAGATCTGGCATCGGTTCCAGATCGTGAGGCTG
AAGATATCAGTGATGAATTACAACTACTGGATCAGGCGGTCAGCTCAGTTCGAACAGAAATTCAGCAGCTTGATGAAAAA
ATGCAAGACTCATTGATGTCTGAAGAGCGTGCATTATTTAGTGTGTTCTTACGCATGCTGGATGAAAATGCGCTCCCTGC
GGAAATCAAGGAATTAATTCGTGCAGGTAATTGGGCACAAGGTGCCGTACGTCGGGTGATCGACAAGCACATTACCCTCT
TTGCCCAAATGGAAGATGACTATTTACGTGAACGTGTTTCAGATTTAAAAGATTTGGGGCGCCGCATTTTAGCCTCACTC
CAGGAAGCCGATTCCAGTCATCGTGAAATTAGTCCAGACAGTATTATTATTGGAGAAGAAATCAGTACGGCGGCACTGGT
TGAATTGCCTGTTGATAATATTGCCGCAATTGTGACCACAGAAGGTGCTGCCAATTCACATATGGTGATTGTGGCGCGAG
CTTTGGGTATTCCCACAGTGGTTGGGGTAACTGAGTTGCCCATTAATACGCTTGATGATGCAGAGATGATTGTCGATGCT
TATCAAGGACGCATCTTTATCAATCCACCACGTCGCATGCGTCAGCGTTATAAAGAAATCCAGAAAGAAGAAGAACAAAT
CGCGAAGGATTTAAAACAGTATGAAACCCGTGATGCCATTACGCCTGATGGTGTTGCAGTACGCTTATATGTCAATACAG
GCTTGATGATTGATGTGGTGCGTGGTGTACAGCGCGGTGCTAAAGGTGTCGGTTTATATCGTAGTGAAATCCCGTTTATG
TTACGCGACCGCTTTCCTGGCGAAGAGGAACAGCGTGCCATGTATCGCCAGCAACTGAGTCACTTTGCCAACAAACCTGT
CACCATGCGTACCCTCGATATTGGTGCCGATAAAGATCTGCCTTATTTTAGTATTGAGGAAGAAAACTCGGCATTGGGCT
GGCGTGGCCTGCGTTTTACGCTGGATCATCCAGAGATTTTCTCGACCCAAGTTCGTGCCATGCTCAAAGCCAGTATTGGC
CTGAATAATTTGCATATTTTGCTGCCAATGGTAACCAGTGTTAGTGAAGTTGAAGAGGTTTTGTATCTGGTAGAACGTGA
CTGGAATGCCGTACAAGAGGAAGAACAGGTCAAAATTACCAAACCGAAAATTGGTATCATGGTCGAAGTCCCAAGTGTAC
TTTTGCAGATTGATGAATTTTCTGAACTGGTCGATTTCTTCTCTGTGGGTTCTAACGATTTGATTCAGTATCTTCTGGCG
GTAGACCGTAACAATCCACGTGTTTCAAGTGTCTACTCTCATTTTCATCCTTCTGTATTACGTGCCTTGCAACGCTTGGT
CAAAGACTGTCATAAGTATGAAAAACCCGTCAGTATTTGTGGAGAAATGGCAGGCGATCCGCTTTCAGCAATTTTACTGA
TGGCGATGGGATTCAACACGCTTTCGATGAGTTCAAGTAATATTTTGCGGGTGCGTAAAGCCATCTGTCACGTCGCCATG
AGCGATGCAGAAAAGCTACTCAGTGAAGTGGTTAAAATGAATAATCCACTGATGGTCAAAAGCTGGATCGAGTATTATTT
TAAAAATCACGGGCTTGGCGACATGGTCAAATCTAACAGATTGGTATCAGTTTAA

Upstream 100 bases:

>100_bases
CAACAAACGTTCGAAAAATCATCTCTCTAGCAAATGATTTTTCGGTCGAGTGCTGTTATAACATCACTATGAAAAATTAA
AAATTTTGGAGTATGCATCC

Downstream 100 bases:

>100_bases
ATTAGGCTTCATCAGAAAAGGGCAAATCATTTGCCCTTTTTTAATTGAAAATTTTATTCTGGATATGCACGCTCCAAAAT
TAGTTTGGAATCTGGCATAA

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Enzyme I-Ntr; Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 764; Mature: 763

Protein sequence:

>764_residues
MSNMQLDTLRRIVQEINSSVSLHDSLDIMVNQVADAMKVDVCSIYLLDERNQRYLLMASKGLNPESVGHVSLQLSEGLVG
LVGQREEIVNLENASKHERFAYLPETGEEIYNSFLGVPVMYRRKVMGVLVVQNKQPQDFSEAAESFLVTLCAQLSGVIAH
AHAVGNIDVFRKPSNGATYKTFQGVPGSGGIALGRAIVLYPPADLASVPDREAEDISDELQLLDQAVSSVRTEIQQLDEK
MQDSLMSEERALFSVFLRMLDENALPAEIKELIRAGNWAQGAVRRVIDKHITLFAQMEDDYLRERVSDLKDLGRRILASL
QEADSSHREISPDSIIIGEEISTAALVELPVDNIAAIVTTEGAANSHMVIVARALGIPTVVGVTELPINTLDDAEMIVDA
YQGRIFINPPRRMRQRYKEIQKEEEQIAKDLKQYETRDAITPDGVAVRLYVNTGLMIDVVRGVQRGAKGVGLYRSEIPFM
LRDRFPGEEEQRAMYRQQLSHFANKPVTMRTLDIGADKDLPYFSIEEENSALGWRGLRFTLDHPEIFSTQVRAMLKASIG
LNNLHILLPMVTSVSEVEEVLYLVERDWNAVQEEEQVKITKPKIGIMVEVPSVLLQIDEFSELVDFFSVGSNDLIQYLLA
VDRNNPRVSSVYSHFHPSVLRALQRLVKDCHKYEKPVSICGEMAGDPLSAILLMAMGFNTLSMSSSNILRVRKAICHVAM
SDAEKLLSEVVKMNNPLMVKSWIEYYFKNHGLGDMVKSNRLVSV

Sequences:

>Translated_764_residues
MSNMQLDTLRRIVQEINSSVSLHDSLDIMVNQVADAMKVDVCSIYLLDERNQRYLLMASKGLNPESVGHVSLQLSEGLVG
LVGQREEIVNLENASKHERFAYLPETGEEIYNSFLGVPVMYRRKVMGVLVVQNKQPQDFSEAAESFLVTLCAQLSGVIAH
AHAVGNIDVFRKPSNGATYKTFQGVPGSGGIALGRAIVLYPPADLASVPDREAEDISDELQLLDQAVSSVRTEIQQLDEK
MQDSLMSEERALFSVFLRMLDENALPAEIKELIRAGNWAQGAVRRVIDKHITLFAQMEDDYLRERVSDLKDLGRRILASL
QEADSSHREISPDSIIIGEEISTAALVELPVDNIAAIVTTEGAANSHMVIVARALGIPTVVGVTELPINTLDDAEMIVDA
YQGRIFINPPRRMRQRYKEIQKEEEQIAKDLKQYETRDAITPDGVAVRLYVNTGLMIDVVRGVQRGAKGVGLYRSEIPFM
LRDRFPGEEEQRAMYRQQLSHFANKPVTMRTLDIGADKDLPYFSIEEENSALGWRGLRFTLDHPEIFSTQVRAMLKASIG
LNNLHILLPMVTSVSEVEEVLYLVERDWNAVQEEEQVKITKPKIGIMVEVPSVLLQIDEFSELVDFFSVGSNDLIQYLLA
VDRNNPRVSSVYSHFHPSVLRALQRLVKDCHKYEKPVSICGEMAGDPLSAILLMAMGFNTLSMSSSNILRVRKAICHVAM
SDAEKLLSEVVKMNNPLMVKSWIEYYFKNHGLGDMVKSNRLVSV
>Mature_763_residues
SNMQLDTLRRIVQEINSSVSLHDSLDIMVNQVADAMKVDVCSIYLLDERNQRYLLMASKGLNPESVGHVSLQLSEGLVGL
VGQREEIVNLENASKHERFAYLPETGEEIYNSFLGVPVMYRRKVMGVLVVQNKQPQDFSEAAESFLVTLCAQLSGVIAHA
HAVGNIDVFRKPSNGATYKTFQGVPGSGGIALGRAIVLYPPADLASVPDREAEDISDELQLLDQAVSSVRTEIQQLDEKM
QDSLMSEERALFSVFLRMLDENALPAEIKELIRAGNWAQGAVRRVIDKHITLFAQMEDDYLRERVSDLKDLGRRILASLQ
EADSSHREISPDSIIIGEEISTAALVELPVDNIAAIVTTEGAANSHMVIVARALGIPTVVGVTELPINTLDDAEMIVDAY
QGRIFINPPRRMRQRYKEIQKEEEQIAKDLKQYETRDAITPDGVAVRLYVNTGLMIDVVRGVQRGAKGVGLYRSEIPFML
RDRFPGEEEQRAMYRQQLSHFANKPVTMRTLDIGADKDLPYFSIEEENSALGWRGLRFTLDHPEIFSTQVRAMLKASIGL
NNLHILLPMVTSVSEVEEVLYLVERDWNAVQEEEQVKITKPKIGIMVEVPSVLLQIDEFSELVDFFSVGSNDLIQYLLAV
DRNNPRVSSVYSHFHPSVLRALQRLVKDCHKYEKPVSICGEMAGDPLSAILLMAMGFNTLSMSSSNILRVRKAICHVAMS
DAEKLLSEVVKMNNPLMVKSWIEYYFKNHGLGDMVKSNRLVSV

Specific function: Component of the phosphoenolpyruvate-dependent nitrogen- metabolic phosphotransferase system (nitrogen-metabolic PTS), that seems to be involved in regulating nitrogen metabolism. Enzyme I- Ntr transfers the phosphoryl group from phosphoenolpyruvate (PEP)

COG id: COG3605

COG function: function code T; Signal transduction protein containing GAF and PtsI domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GAF domain [H]

Homologues:

Organism=Escherichia coli, GI1789193, Length=754, Percent_Identity=37.4005305039788, Blast_Score=533, Evalue=1e-152,
Organism=Escherichia coli, GI1788756, Length=551, Percent_Identity=32.6678765880218, Blast_Score=298, Evalue=6e-82,
Organism=Escherichia coli, GI1788726, Length=500, Percent_Identity=33.4, Blast_Score=254, Evalue=1e-68,
Organism=Escherichia coli, GI48994992, Length=453, Percent_Identity=31.3465783664459, Blast_Score=243, Evalue=4e-65,
Organism=Escherichia coli, GI1787994, Length=405, Percent_Identity=25.1851851851852, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003018
- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF01590 GAF; PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 85545; Mature: 85414

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00742 PEP_ENZYMES_2 ; PS00449 ATPASE_A

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNMQLDTLRRIVQEINSSVSLHDSLDIMVNQVADAMKVDVCSIYLLDERNQRYLLMASK
CCCCCHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHEEEEEEEECCCCEEEEEECC
GLNPESVGHVSLQLSEGLVGLVGQREEIVNLENASKHERFAYLPETGEEIYNSFLGVPVM
CCCCCCCCEEEEEECCCCHHHCCCHHHHHCCCCCCCCCCEEECCCCHHHHHHHHCCCCHH
YRRKVMGVLVVQNKQPQDFSEAAESFLVTLCAQLSGVIAHAHAVGNIDVFRKPSNGATYK
HHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCEE
TFQGVPGSGGIALGRAIVLYPPADLASVPDREAEDISDELQLLDQAVSSVRTEIQQLDEK
EECCCCCCCCHHHCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MQDSLMSEERALFSVFLRMLDENALPAEIKELIRAGNWAQGAVRRVIDKHITLFAQMEDD
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHEECCHH
YLRERVSDLKDLGRRILASLQEADSSHREISPDSIIIGEEISTAALVELPVDNIAAIVTT
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCEEEECCCCCEEEEEEE
EGAANSHMVIVARALGIPTVVGVTELPINTLDDAEMIVDAYQGRIFINPPRRMRQRYKEI
CCCCCCCEEEEEEHHCCCCEECCCCCCCCCCCHHHHHHHHHCCEEEECCHHHHHHHHHHH
QKEEEQIAKDLKQYETRDAITPDGVAVRLYVNTGLMIDVVRGVQRGAKGVGLYRSEIPFM
HHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCCCHHHCCCHH
LRDRFPGEEEQRAMYRQQLSHFANKPVTMRTLDIGADKDLPYFSIEEENSALGWRGLRFT
HHCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEECCCCCCCCCCCEEEE
LDHPEIFSTQVRAMLKASIGLNNLHILLPMVTSVSEVEEVLYLVERDWNAVQEEEQVKIT
ECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCEEEE
KPKIGIMVEVPSVLLQIDEFSELVDFFSVGSNDLIQYLLAVDRNNPRVSSVYSHFHPSVL
CCCEEEEEECHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHCCHHHH
RALQRLVKDCHKYEKPVSICGEMAGDPLSAILLMAMGFNTLSMSSSNILRVRKAICHVAM
HHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
SDAEKLLSEVVKMNNPLMVKSWIEYYFKNHGLGDMVKSNRLVSV
HHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCHHHHHCCCCCCC
>Mature Secondary Structure 
SNMQLDTLRRIVQEINSSVSLHDSLDIMVNQVADAMKVDVCSIYLLDERNQRYLLMASK
CCCCHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHEEEEEEEECCCCEEEEEECC
GLNPESVGHVSLQLSEGLVGLVGQREEIVNLENASKHERFAYLPETGEEIYNSFLGVPVM
CCCCCCCCEEEEEECCCCHHHCCCHHHHHCCCCCCCCCCEEECCCCHHHHHHHHCCCCHH
YRRKVMGVLVVQNKQPQDFSEAAESFLVTLCAQLSGVIAHAHAVGNIDVFRKPSNGATYK
HHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCEE
TFQGVPGSGGIALGRAIVLYPPADLASVPDREAEDISDELQLLDQAVSSVRTEIQQLDEK
EECCCCCCCCHHHCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MQDSLMSEERALFSVFLRMLDENALPAEIKELIRAGNWAQGAVRRVIDKHITLFAQMEDD
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHEECCHH
YLRERVSDLKDLGRRILASLQEADSSHREISPDSIIIGEEISTAALVELPVDNIAAIVTT
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCEEEECCCCCEEEEEEE
EGAANSHMVIVARALGIPTVVGVTELPINTLDDAEMIVDAYQGRIFINPPRRMRQRYKEI
CCCCCCCEEEEEEHHCCCCEECCCCCCCCCCCHHHHHHHHHCCEEEECCHHHHHHHHHHH
QKEEEQIAKDLKQYETRDAITPDGVAVRLYVNTGLMIDVVRGVQRGAKGVGLYRSEIPFM
HHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCCCHHHCCCHH
LRDRFPGEEEQRAMYRQQLSHFANKPVTMRTLDIGADKDLPYFSIEEENSALGWRGLRFT
HHCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEECCCCCCCCCCCEEEE
LDHPEIFSTQVRAMLKASIGLNNLHILLPMVTSVSEVEEVLYLVERDWNAVQEEEQVKIT
ECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCEEEE
KPKIGIMVEVPSVLLQIDEFSELVDFFSVGSNDLIQYLLAVDRNNPRVSSVYSHFHPSVL
CCCEEEEEECHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHCCHHHH
RALQRLVKDCHKYEKPVSICGEMAGDPLSAILLMAMGFNTLSMSSSNILRVRKAICHVAM
HHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
SDAEKLLSEVVKMNNPLMVKSWIEYYFKNHGLGDMVKSNRLVSV
HHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 7896715; 8973315 [H]