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The map label for this gene is hcaR [H]

Identifier: 50083697

GI number: 50083697

Start: 444028

End: 444924

Strand: Reverse

Name: hcaR [H]

Synonym: ACIAD0448

Alternate gene names: 50083697

Gene position: 444924-444028 (Counterclockwise)

Preceding gene: 50083700

Following gene: 50083696

Centisome position: 12.36

GC content: 40.36

Gene sequence:

>897_bases
GTGGAATTGCGTCATCTCCGATATTTCGTCACTGTAGCTGAAGAGCTTAACTTTAGTAAAGCTGCCTTAAAACTTTATAC
TGCTCAACCCTCACTTAGCCAACAGATCAAAGATTTGGAAGAAGACGTCGGTGTACAACTTCTTTATCGAACCAAACGTA
AAGTGGAGCTCACAGAAGAAGGCGCTGTATTTTTAGAACAGGCTCGGCTTACTTTGGCTCAGGCAGATAAAGCGGTCATG
ATGGCACGCAAAGTCTCTCAGGCAAAGCAACAATGTTTGCGCATCGGTTTTGTTCCTGTTGCAGAAGTACAAGTATTTCC
GCATGTGTTACCTAATTTTCGTGCGCATCATCCAGAACTTAGAATTGAACTCCATAGTTTAAATAACGATCAGCAACTTA
AGGCGCTTAAACGTGGTGAACTCGATTTATGTTTTATCCGAGATAATATTCAGCATCGTGATGGTATCCATAGTCAATTG
GTATTAAGTGAACCACTCATTTTTATCCTGCCTAAACAGCATCCATTGGCCAAACTTGAACGCATTCCGCTTGAAGCATT
AAACAATATCGATTTTATTATTCCGGCAGCAGATCAGGCCAGAGTGTTAAACCAGCATATTTTGCAGTTTGCCAAACAAC
ATCAGATCCAGTTCAACATTATTCAAAGTGCTGATACTGTCTTATCCAACATTACTTCTGTGGCACTCAATCTGGGCTGT
AGTATTTTACCTAATTATGTGTCAACACTCTTGCCAGAAAATTGTGTGATGCGACCACTTGATATTGAATTGCCACATTT
TGATTTATTTATGGCTTACCAATCAGCATCAAAAACGCCACCAGTAACGACATTTATCAAGGCATTGGACAAATGGTTTC
AACCTGCTGGCTATTGA

Upstream 100 bases:

>100_bases
ATACTATTTATTTTATAAATGACAAAAGCGGTTTTAAAGATTTTCTATTGATTTAATATACTTTAGACTGGCTCAAGCAA
GACATTTCTGGATTAACTTA

Downstream 100 bases:

>100_bases
CCATTTTAAATGATTTTTATGGTCATAGATCTACGCATTCGATTTCAGACAAGAATCATTTATTTATGCTATACATTTGA
AAATCACAGCATTGCCTAAG

Product: DNA-binding transcriptional regulator HcaR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MELRHLRYFVTVAEELNFSKAALKLYTAQPSLSQQIKDLEEDVGVQLLYRTKRKVELTEEGAVFLEQARLTLAQADKAVM
MARKVSQAKQQCLRIGFVPVAEVQVFPHVLPNFRAHHPELRIELHSLNNDQQLKALKRGELDLCFIRDNIQHRDGIHSQL
VLSEPLIFILPKQHPLAKLERIPLEALNNIDFIIPAADQARVLNQHILQFAKQHQIQFNIIQSADTVLSNITSVALNLGC
SILPNYVSTLLPENCVMRPLDIELPHFDLFMAYQSASKTPPVTTFIKALDKWFQPAGY

Sequences:

>Translated_298_residues
MELRHLRYFVTVAEELNFSKAALKLYTAQPSLSQQIKDLEEDVGVQLLYRTKRKVELTEEGAVFLEQARLTLAQADKAVM
MARKVSQAKQQCLRIGFVPVAEVQVFPHVLPNFRAHHPELRIELHSLNNDQQLKALKRGELDLCFIRDNIQHRDGIHSQL
VLSEPLIFILPKQHPLAKLERIPLEALNNIDFIIPAADQARVLNQHILQFAKQHQIQFNIIQSADTVLSNITSVALNLGC
SILPNYVSTLLPENCVMRPLDIELPHFDLFMAYQSASKTPPVTTFIKALDKWFQPAGY
>Mature_298_residues
MELRHLRYFVTVAEELNFSKAALKLYTAQPSLSQQIKDLEEDVGVQLLYRTKRKVELTEEGAVFLEQARLTLAQADKAVM
MARKVSQAKQQCLRIGFVPVAEVQVFPHVLPNFRAHHPELRIELHSLNNDQQLKALKRGELDLCFIRDNIQHRDGIHSQL
VLSEPLIFILPKQHPLAKLERIPLEALNNIDFIIPAADQARVLNQHILQFAKQHQIQFNIIQSADTVLSNITSVALNLGC
SILPNYVSTLLPENCVMRPLDIELPHFDLFMAYQSASKTPPVTTFIKALDKWFQPAGY

Specific function: Transcriptional activator of the hca operon for 3- phenylpropionic acid catabolism [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788887, Length=286, Percent_Identity=41.958041958042, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI1787879, Length=245, Percent_Identity=35.5102040816327, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI2367136, Length=293, Percent_Identity=26.6211604095563, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI1790399, Length=244, Percent_Identity=28.6885245901639, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI157672245, Length=246, Percent_Identity=26.0162601626016, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1788297, Length=245, Percent_Identity=27.7551020408163, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1788748, Length=252, Percent_Identity=25.7936507936508, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI145693105, Length=245, Percent_Identity=26.1224489795918, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1790262, Length=248, Percent_Identity=28.2258064516129, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI87081904, Length=177, Percent_Identity=28.8135593220339, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1787530, Length=194, Percent_Identity=27.319587628866, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1790208, Length=265, Percent_Identity=26.0377358490566, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787806, Length=295, Percent_Identity=21.6949152542373, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787601, Length=143, Percent_Identity=27.972027972028, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789204, Length=269, Percent_Identity=22.6765799256506, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI145693193, Length=152, Percent_Identity=32.8947368421053, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787589, Length=123, Percent_Identity=34.9593495934959, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790783, Length=145, Percent_Identity=26.2068965517241, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33969; Mature: 33969

Theoretical pI: Translated: 7.69; Mature: 7.69

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELRHLRYFVTVAEELNFSKAALKLYTAQPSLSQQIKDLEEDVGVQLLYRTKRKVELTEE
CCCHHHHHHHHHHHHCCCCHHHHEEEECCCCHHHHHHHHHHHHCCEEEECCCCCEEECCC
GAVFLEQARLTLAQADKAVMMARKVSQAKQQCLRIGFVPVAEVQVFPHVLPNFRAHHPEL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCEE
RIELHSLNNDQQLKALKRGELDLCFIRDNIQHRDGIHSQLVLSEPLIFILPKQHPLAKLE
EEEEECCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHCCCEEEEECCCCCHHHHH
RIPLEALNNIDFIIPAADQARVLNQHILQFAKQHQIQFNIIQSADTVLSNITSVALNLGC
HCCHHHHCCCCEEEECCHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHCCC
SILPNYVSTLLPENCVMRPLDIELPHFDLFMAYQSASKTPPVTTFIKALDKWFQPAGY
HHHHHHHHHHCCHHHCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MELRHLRYFVTVAEELNFSKAALKLYTAQPSLSQQIKDLEEDVGVQLLYRTKRKVELTEE
CCCHHHHHHHHHHHHCCCCHHHHEEEECCCCHHHHHHHHHHHHCCEEEECCCCCEEECCC
GAVFLEQARLTLAQADKAVMMARKVSQAKQQCLRIGFVPVAEVQVFPHVLPNFRAHHPEL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCEE
RIELHSLNNDQQLKALKRGELDLCFIRDNIQHRDGIHSQLVLSEPLIFILPKQHPLAKLE
EEEEECCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHCCCEEEEECCCCCHHHHH
RIPLEALNNIDFIIPAADQARVLNQHILQFAKQHQIQFNIIQSADTVLSNITSVALNLGC
HCCHHHHCCCCEEEECCHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHCCC
SILPNYVSTLLPENCVMRPLDIELPHFDLFMAYQSASKTPPVTTFIKALDKWFQPAGY
HHHHHHHHHHCCHHHCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 9603882 [H]