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The map label for this gene is norM

Identifier: 50083683

GI number: 50083683

Start: 427750

End: 429099

Strand: Reverse

Name: norM

Synonym: ACIAD0429

Alternate gene names: 50083683

Gene position: 429099-427750 (Counterclockwise)

Preceding gene: 50083684

Following gene: 50083680

Centisome position: 11.92

GC content: 46.37

Gene sequence:

>1350_bases
GTGGCAAAAGTTGCGGGCTTCCGCTTTGAGTTAAAACAGCTATTCCACCTGATGTGGCCAATTTTGATTACGCAATTTGC
GCAAGCAGGTTTGGGGCTCATCGATACCATCATGGCAGGACATTTATCTGCCAATGATCTGGCTGCAATTGCGGTTGGTG
TTGGCTTATGGATGCCAGTCATGCTGCTATTTAGTGCCATCATGATTGCAACTACCCCCTTGGTCGCGGAAGCAAAAGGC
GCCAGAACGCCAGAACATATTCCTGTTATTGTTCGCCAGTCATTGTGGGTTGCGGTTTCACTTGGTGTGATCGCGATGCT
GATTTTACAACTCATGCCTTTTTTACTGCCGATCTTGGGCGTTCCCGAAAGTTTACAGCCCAAAGCTGGCTTGTTTTTAC
ATGCCATTGGTTTTGGCATGCCCGCAGTGACCATGTATGCTGCGTTACGGGGCTATTCCGAGGCACTTGGCTATCCCCGA
CCAGTCACCGTCATCAGTCTATTAGCACTGGTTGTGCTGGTTCCACTGAATTATATTTTTATGTATGGTATCGGCCCTGT
ACCGCACTTAGGCAGTGCAGGCTGTGGTTTTGCAACTGCCATTTTACAATGGCTGATGCTGATCACACTGGCCAGCTATA
TATATCGTGCCAAAGCCTATCAAAGCACTCAAGTGTTTAGTCATTGGGAAAGAATCAATCTCACGTTAGTCAAACGTATT
TTAAAACTCGGTTTGCCCATTGGATTAGCGGTATTTTTTGAAGTCAGTATCTTTAGTACAGGGGCAATTGTATTAAGTCC
ACTGGGTGATACATTGGTCGCTGCGCATCAGATTGCCATGTCGGTCACATCGCAACTGTTTATGATTCCGATGTCACTGG
CGATTGCACTGACCATTCGGGTGGGAATGTATTATGGAGAAAAAAACTGGGTATCCATGCGTCTCGTACAGAAACTCGGA
CTTGCAACTGCCACTTTTTTTGCTATGTGTACCATGAGCCTGATCTGGTTTGCACGCCCACAAATTGTCGCAATTTATAC
CCAAGATCCAGCCGTATTTGATATTGCTTTATATTTACTACTTTTTGCGATGGCTTATCAATTGATGGATGCCTGGCAAG
TGGGTGCCGCAGGCTGTCTTCGTGGCATGCAGGACACCAAAGGTCCGATGTGGATCACCCTGATTGCGTATTGGGTCGTT
GCATTTCCAGTTGGAACTTATCTCGCACGTGTCGCAAAAATGGGGCCTGCGGGTGTTTGGTTGGGTTTAATCACTGGTTT
AAGCATTGCCTGCGTTTTACTTTTGATGCGTTTATATCGCAATAACCATAAGTTGGCGCAACAATCATAA

Upstream 100 bases:

>100_bases
CCTGTAAACTGGCAGCGGCTAATTGGGGCACTGATGATTTTTATTGGGGTAATTTTTACCTTTCAACGCTAAATTTTATA
TTGATAGATGAGTATTTTTT

Downstream 100 bases:

>100_bases
AGAAAGAAATCCTTCCAACGGATTAAGGTCATCAATCCATTGAAAGGATTTGAAGCAGAATATCAGGCAGATTTTGCTTG
TGGACTTGCGCCAATTTGCC

Product: multidrug ABC transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MAKVAGFRFELKQLFHLMWPILITQFAQAGLGLIDTIMAGHLSANDLAAIAVGVGLWMPVMLLFSAIMIATTPLVAEAKG
ARTPEHIPVIVRQSLWVAVSLGVIAMLILQLMPFLLPILGVPESLQPKAGLFLHAIGFGMPAVTMYAALRGYSEALGYPR
PVTVISLLALVVLVPLNYIFMYGIGPVPHLGSAGCGFATAILQWLMLITLASYIYRAKAYQSTQVFSHWERINLTLVKRI
LKLGLPIGLAVFFEVSIFSTGAIVLSPLGDTLVAAHQIAMSVTSQLFMIPMSLAIALTIRVGMYYGEKNWVSMRLVQKLG
LATATFFAMCTMSLIWFARPQIVAIYTQDPAVFDIALYLLLFAMAYQLMDAWQVGAAGCLRGMQDTKGPMWITLIAYWVV
AFPVGTYLARVAKMGPAGVWLGLITGLSIACVLLLMRLYRNNHKLAQQS

Sequences:

>Translated_449_residues
MAKVAGFRFELKQLFHLMWPILITQFAQAGLGLIDTIMAGHLSANDLAAIAVGVGLWMPVMLLFSAIMIATTPLVAEAKG
ARTPEHIPVIVRQSLWVAVSLGVIAMLILQLMPFLLPILGVPESLQPKAGLFLHAIGFGMPAVTMYAALRGYSEALGYPR
PVTVISLLALVVLVPLNYIFMYGIGPVPHLGSAGCGFATAILQWLMLITLASYIYRAKAYQSTQVFSHWERINLTLVKRI
LKLGLPIGLAVFFEVSIFSTGAIVLSPLGDTLVAAHQIAMSVTSQLFMIPMSLAIALTIRVGMYYGEKNWVSMRLVQKLG
LATATFFAMCTMSLIWFARPQIVAIYTQDPAVFDIALYLLLFAMAYQLMDAWQVGAAGCLRGMQDTKGPMWITLIAYWVV
AFPVGTYLARVAKMGPAGVWLGLITGLSIACVLLLMRLYRNNHKLAQQS
>Mature_448_residues
AKVAGFRFELKQLFHLMWPILITQFAQAGLGLIDTIMAGHLSANDLAAIAVGVGLWMPVMLLFSAIMIATTPLVAEAKGA
RTPEHIPVIVRQSLWVAVSLGVIAMLILQLMPFLLPILGVPESLQPKAGLFLHAIGFGMPAVTMYAALRGYSEALGYPRP
VTVISLLALVVLVPLNYIFMYGIGPVPHLGSAGCGFATAILQWLMLITLASYIYRAKAYQSTQVFSHWERINLTLVKRIL
KLGLPIGLAVFFEVSIFSTGAIVLSPLGDTLVAAHQIAMSVTSQLFMIPMSLAIALTIRVGMYYGEKNWVSMRLVQKLGL
ATATFFAMCTMSLIWFARPQIVAIYTQDPAVFDIALYLLLFAMAYQLMDAWQVGAAGCLRGMQDTKGPMWITLIAYWVVA
FPVGTYLARVAKMGPAGVWLGLITGLSIACVLLLMRLYRNNHKLAQQS

Specific function: Multidrug efflux pump

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Homologues:

Organism=Homo sapiens, GI22907060, Length=452, Percent_Identity=23.6725663716814, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI153792564, Length=462, Percent_Identity=23.3766233766234, Blast_Score=86, Evalue=9e-17,
Organism=Homo sapiens, GI38261960, Length=492, Percent_Identity=22.3577235772358, Blast_Score=76, Evalue=7e-14,
Organism=Escherichia coli, GI48994890, Length=438, Percent_Identity=38.1278538812785, Blast_Score=289, Evalue=2e-79,
Organism=Escherichia coli, GI226510954, Length=440, Percent_Identity=22.7272727272727, Blast_Score=74, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6320545, Length=429, Percent_Identity=21.4452214452214, Blast_Score=77, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6321821, Length=410, Percent_Identity=21.219512195122, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NORM_ACIAD (Q6FEY7)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_045193.1
- STRING:   Q6FEY7
- GeneID:   2879461
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD0429
- NMPDR:   fig|62977.3.peg.1030
- eggNOG:   COG0534
- HOGENOM:   HBG479615
- OMA:   KQLFHLM
- PhylomeDB:   Q6FEY7
- ProtClustDB:   CLSK830471
- BioCyc:   ASP62977:ACIAD0429-MONOMER
- InterPro:   IPR002528
- PANTHER:   PTHR11206
- TIGRFAMs:   TIGR00797

Pfam domain/function: PF01554 MatE

EC number: NA

Molecular weight: Translated: 49014; Mature: 48883

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x15b84574)-; HASH(0x166d8630)-; HASH(0x166c516c)-; HASH(0x166d9710)-; HASH(0x15e6a974)-; HASH(0x166e8158)-; HASH(0x166d86e4)-; HASH(0x166c2918)-; HASH(0x166d8750)-; HASH(0x166cbb5c)-; HASH(0x161ac964)-; HASH(0x151d9f18)-;

Cys/Met content:

0.9 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKVAGFRFELKQLFHLMWPILITQFAQAGLGLIDTIMAGHLSANDLAAIAVGVGLWMPV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
MLLFSAIMIATTPLVAEAKGARTPEHIPVIVRQSLWVAVSLGVIAMLILQLMPFLLPILG
HHHHHHHHHHHCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
VPESLQPKAGLFLHAIGFGMPAVTMYAALRGYSEALGYPRPVTVISLLALVVLVPLNYIF
CCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
MYGIGPVPHLGSAGCGFATAILQWLMLITLASYIYRAKAYQSTQVFSHWERINLTLVKRI
HHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKLGLPIGLAVFFEVSIFSTGAIVLSPLGDTLVAAHQIAMSVTSQLFMIPMSLAIALTIR
HHHCCCHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGMYYGEKNWVSMRLVQKLGLATATFFAMCTMSLIWFARPQIVAIYTQDPAVFDIALYLL
HHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHH
LFAMAYQLMDAWQVGAAGCLRGMQDTKGPMWITLIAYWVVAFPVGTYLARVAKMGPAGVW
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
LGLITGLSIACVLLLMRLYRNNHKLAQQS
HHHHHHHHHHHHHHHHHHHHCCCCCCCCH
>Mature Secondary Structure 
AKVAGFRFELKQLFHLMWPILITQFAQAGLGLIDTIMAGHLSANDLAAIAVGVGLWMPV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHH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HHHHHHHHHHHHHHHHHHHHCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA