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The map label for this gene is priA [H]

Identifier: 50083665

GI number: 50083665

Start: 410989

End: 413220

Strand: Reverse

Name: priA [H]

Synonym: ACIAD0409

Alternate gene names: 50083665

Gene position: 413220-410989 (Counterclockwise)

Preceding gene: 50083670

Following gene: 50083664

Centisome position: 11.48

GC content: 44.85

Gene sequence:

>2232_bases
ATGCCACTAACGCCACCCCAGCCTTCAACTGAAATTTATCGTGTTCGTGTGGCTGTTCCTGTGCATTTATACGATACTTT
CGACTATAGCGTAAGCCAGCAACAATATGAACAGGCACAAGCTGGCGCACGCGTACTGGTTTCATTTGGCAGACAATCAC
TGATTGGGGTAATTACTGAAAAACTCGCGCCAGATCTTCCCATAGACCCGCGTTTTCAATTAAAAACAATACGCGAACTG
ATCGATCAAGCGCCTATTTTAGATACCAAAGTTTTAACTTTATTGACATGGTCGGCACATTATTATCAATTTCCGATCGG
TGAAGTCATTCAGACAGCGCTTCCAAGTTTACTCAGACAAGGTAAATCTCAAGATGTTTTAACTTATATCTGGAAAATTA
CCGCACATCCAGAGGCCGAATCAAAATTAAAACGTTCACAAAAACAGTATGATGCCTATCAAATTTTAAAGCTTCATCCC
ACTGGAACGACAGAAACTATTTTAAACTTGAGTGGTGTAGAAACTGCAACCTTACGTGCACTGGCCAAAAAAGAATTGGT
TGCTTGTACACAGGAAATTCAAGACTTTACCCCGATGCCTGTGCAACTGGCACAAATGCCGCTCACACCCAATCCAGATC
AGCACAATGCAATCCAACGTGTATTAAAGTCCCTGCATCATTATCAGGCATTTTTGCTAGATGGCTTGACGGGTAGCGGC
AAAACGGAAGTCTATCTTCAGATCATGCATGAAGTCCTCAAGCAGGGAAAACAAGTGCTGGTGCTGGTTCCAGAGATTGG
ACTGACACCACAGACCATCTCACGTTTTAAATCCCGCTTTCATTGTGATGTTGCTTTATTACATTCTGGACTCAATGATT
CCAAACGATTACAGGCATGGCAACAAGCCAAAATGGGTAAAGCCTCGATTATTTTAGGAACACGCTCGGCCATTTATACG
CCATTACCACGACTGGGCTTAATTATTCTGGATGAAGAACATGATTTGTCTTATAAGCAACAGGAAGGTTTTCGCTATCA
TGCGCGAGACGTTGCCCTATATCGTGGTTATTTAGAACAATGCCCTGTAATATTAGGCTCGGCGACCCCCAGTATAGACA
GTTATCATCTGGTGGAACAAAATAAGCTTGAGCTACTCCAGCTCAATCAGCGTGCTGGTGTCGCGCTATTACCTAAAATG
CACCTGATCGATCTCAAGGTTGCCAAAAAGAATGAGGGACTCAGCCAGATTCTCATCGATCAGATTCAACACACACTGGA
TCGTCAGGAACAGGTGCTGATTTTTTTAAATCGACGTGGCTATGCGCCTGTTCTGGTCTGTGAAAGCTGTGGCTGGCAAG
CCAATTGTCCAAATTGCGATGCGCATTTCACATTACACACTCAGCCTTATGGTTATTTACATTGTCATCATTGTGGAACG
ATTCATCGTTTGCCCGAGACCTGTCCCGAATGTCATCATCACACCTTAAAAACGCTGGGCATGGGAACAGCAAAGGTGGA
AGAACATCTGAAAACACTGTTTCCTGAACACGATGTGATTCGAGTCGATCGTGACAGTACCAGTCGTGTCGGAAGCTGGC
AAAAAATCTATGACCGAATCCAGCACAATAAACCCATGATTCTGTTAGGCACACAAATGCTGGCCAAGGGGCATCATTTC
CCTTATGTAACGCTAGTGGCTATTCTGGATATTGATGCAGGGTTATTAAGTGTCGATGTTCGCGCACCTGAACGAACAGC
TCAATTAATTGTGCAGGTGGCAGGTCGTGCAGGTCGCGGTGAACATAAAGGTCATGTCTATATGCAAACCTTGCGTCCAG
ATCATCCGATGCTGACGACACTGATTGAAAAAGACTATCGGGCTGTGGCGAAAGATATGCTTGAAGAGCGAAAAGCAGCA
CGATTACCGCCTTATCGCTACAGTGTACTCATTCGATGTGAATCACGTGATCGGGATTATAGTCAGGCTTTCTTGCAAGA
GATTGCGCAGATGCTTCAGCATCAAGCGGGCGACAGTATAGAAATTTGGGGACCCATTCCTGCCCCGATGGAACGTAAAG
CAGGACGTTATCGGGCACATATGGTGATCTTGGCGCAAGATCGGGCGCGTCTACATTTCTTCTTGCGTCCATGGTGGGCT
GAGGTGATTCAACTCCCTCGTCAGCATCAATTACGTTTATCAATTGATGTTGATCCACAGGAATTTAACTGA

Upstream 100 bases:

>100_bases
GTTGCATCTTTCCCTCAGAATCCTTCATATTTTGGGGAATTTGCTCACACTGTGTGAGTATAGTCCTGTTTAACCCCAAT
TAGAGTGACGAAATAAATTT

Downstream 100 bases:

>100_bases
AATTTAAACATCATGGGTGGATAATGCTAAAGTTTTATCCACTGCTATATGATTGATTGCCTCAGAGTCGCCCATGTCCT
TACTGTTACAAATCACCATT

Product: primosomal protein N'

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 743; Mature: 742

Protein sequence:

>743_residues
MPLTPPQPSTEIYRVRVAVPVHLYDTFDYSVSQQQYEQAQAGARVLVSFGRQSLIGVITEKLAPDLPIDPRFQLKTIREL
IDQAPILDTKVLTLLTWSAHYYQFPIGEVIQTALPSLLRQGKSQDVLTYIWKITAHPEAESKLKRSQKQYDAYQILKLHP
TGTTETILNLSGVETATLRALAKKELVACTQEIQDFTPMPVQLAQMPLTPNPDQHNAIQRVLKSLHHYQAFLLDGLTGSG
KTEVYLQIMHEVLKQGKQVLVLVPEIGLTPQTISRFKSRFHCDVALLHSGLNDSKRLQAWQQAKMGKASIILGTRSAIYT
PLPRLGLIILDEEHDLSYKQQEGFRYHARDVALYRGYLEQCPVILGSATPSIDSYHLVEQNKLELLQLNQRAGVALLPKM
HLIDLKVAKKNEGLSQILIDQIQHTLDRQEQVLIFLNRRGYAPVLVCESCGWQANCPNCDAHFTLHTQPYGYLHCHHCGT
IHRLPETCPECHHHTLKTLGMGTAKVEEHLKTLFPEHDVIRVDRDSTSRVGSWQKIYDRIQHNKPMILLGTQMLAKGHHF
PYVTLVAILDIDAGLLSVDVRAPERTAQLIVQVAGRAGRGEHKGHVYMQTLRPDHPMLTTLIEKDYRAVAKDMLEERKAA
RLPPYRYSVLIRCESRDRDYSQAFLQEIAQMLQHQAGDSIEIWGPIPAPMERKAGRYRAHMVILAQDRARLHFFLRPWWA
EVIQLPRQHQLRLSIDVDPQEFN

Sequences:

>Translated_743_residues
MPLTPPQPSTEIYRVRVAVPVHLYDTFDYSVSQQQYEQAQAGARVLVSFGRQSLIGVITEKLAPDLPIDPRFQLKTIREL
IDQAPILDTKVLTLLTWSAHYYQFPIGEVIQTALPSLLRQGKSQDVLTYIWKITAHPEAESKLKRSQKQYDAYQILKLHP
TGTTETILNLSGVETATLRALAKKELVACTQEIQDFTPMPVQLAQMPLTPNPDQHNAIQRVLKSLHHYQAFLLDGLTGSG
KTEVYLQIMHEVLKQGKQVLVLVPEIGLTPQTISRFKSRFHCDVALLHSGLNDSKRLQAWQQAKMGKASIILGTRSAIYT
PLPRLGLIILDEEHDLSYKQQEGFRYHARDVALYRGYLEQCPVILGSATPSIDSYHLVEQNKLELLQLNQRAGVALLPKM
HLIDLKVAKKNEGLSQILIDQIQHTLDRQEQVLIFLNRRGYAPVLVCESCGWQANCPNCDAHFTLHTQPYGYLHCHHCGT
IHRLPETCPECHHHTLKTLGMGTAKVEEHLKTLFPEHDVIRVDRDSTSRVGSWQKIYDRIQHNKPMILLGTQMLAKGHHF
PYVTLVAILDIDAGLLSVDVRAPERTAQLIVQVAGRAGRGEHKGHVYMQTLRPDHPMLTTLIEKDYRAVAKDMLEERKAA
RLPPYRYSVLIRCESRDRDYSQAFLQEIAQMLQHQAGDSIEIWGPIPAPMERKAGRYRAHMVILAQDRARLHFFLRPWWA
EVIQLPRQHQLRLSIDVDPQEFN
>Mature_742_residues
PLTPPQPSTEIYRVRVAVPVHLYDTFDYSVSQQQYEQAQAGARVLVSFGRQSLIGVITEKLAPDLPIDPRFQLKTIRELI
DQAPILDTKVLTLLTWSAHYYQFPIGEVIQTALPSLLRQGKSQDVLTYIWKITAHPEAESKLKRSQKQYDAYQILKLHPT
GTTETILNLSGVETATLRALAKKELVACTQEIQDFTPMPVQLAQMPLTPNPDQHNAIQRVLKSLHHYQAFLLDGLTGSGK
TEVYLQIMHEVLKQGKQVLVLVPEIGLTPQTISRFKSRFHCDVALLHSGLNDSKRLQAWQQAKMGKASIILGTRSAIYTP
LPRLGLIILDEEHDLSYKQQEGFRYHARDVALYRGYLEQCPVILGSATPSIDSYHLVEQNKLELLQLNQRAGVALLPKMH
LIDLKVAKKNEGLSQILIDQIQHTLDRQEQVLIFLNRRGYAPVLVCESCGWQANCPNCDAHFTLHTQPYGYLHCHHCGTI
HRLPETCPECHHHTLKTLGMGTAKVEEHLKTLFPEHDVIRVDRDSTSRVGSWQKIYDRIQHNKPMILLGTQMLAKGHHFP
YVTLVAILDIDAGLLSVDVRAPERTAQLIVQVAGRAGRGEHKGHVYMQTLRPDHPMLTTLIEKDYRAVAKDMLEERKAAR
LPPYRYSVLIRCESRDRDYSQAFLQEIAQMLQHQAGDSIEIWGPIPAPMERKAGRYRAHMVILAQDRARLHFFLRPWWAE
VIQLPRQHQLRLSIDVDPQEFN

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=734, Percent_Identity=43.8692098092643, Blast_Score=584, Evalue=1e-168,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 84720; Mature: 84589

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLTPPQPSTEIYRVRVAVPVHLYDTFDYSVSQQQYEQAQAGARVLVSFGRQSLIGVITE
CCCCCCCCCCCEEEEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHH
KLAPDLPIDPRFQLKTIRELIDQAPILDTKVLTLLTWSAHYYQFPIGEVIQTALPSLLRQ
HHCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHEECCCEEECCHHHHHHHHHHHHHHC
GKSQDVLTYIWKITAHPEAESKLKRSQKQYDAYQILKLHPTGTTETILNLSGVETATLRA
CCCCCCEEEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCHHHHEECCCCCHHHHHH
LAKKELVACTQEIQDFTPMPVQLAQMPLTPNPDQHNAIQRVLKSLHHYQAFLLDGLTGSG
HHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
KTEVYLQIMHEVLKQGKQVLVLVPEIGLTPQTISRFKSRFHCDVALLHSGLNDSKRLQAW
CHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHH
QQAKMGKASIILGTRSAIYTPLPRLGLIILDEEHDLSYKQQEGFRYHARDVALYRGYLEQ
HHHCCCCEEEEEECCHHHHCCCCCCCEEEEECCCCCCCHHHCCCEEHHHHHHHHHHHHHH
CPVILGSATPSIDSYHLVEQNKLELLQLNQRAGVALLPKMHLIDLKVAKKNEGLSQILID
CCEEEECCCCCCCCEEECCCCCHHHHEECCCCCEEECCCEEEEEEEEECCCCCHHHHHHH
QIQHTLDRQEQVLIFLNRRGYAPVLVCESCGWQANCPNCDAHFTLHTQPYGYLHCHHCGT
HHHHHHCCCCEEEEEEECCCCCCEEEECCCCCCCCCCCCCCEEEEECCCCCEEEEECCCC
IHRLPETCPECHHHTLKTLGMGTAKVEEHLKTLFPEHDVIRVDRDSTSRVGSWQKIYDRI
HHHCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHH
QHNKPMILLGTQMLAKGHHFPYVTLVAILDIDAGLLSVDVRAPERTAQLIVQVAGRAGRG
HCCCCEEEEEHHHHHCCCCCCHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCCC
EHKGHVYMQTLRPDHPMLTTLIEKDYRAVAKDMLEERKAARLPPYRYSVLIRCESRDRDY
CCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCHH
SQAFLQEIAQMLQHQAGDSIEIWGPIPAPMERKAGRYRAHMVILAQDRARLHFFLRPWWA
HHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHCCCCEEEEEEEEECCCCEEEEEECHHHH
EVIQLPRQHQLRLSIDVDPQEFN
HHHHCCCCCCEEEEEECCCCCCC
>Mature Secondary Structure 
PLTPPQPSTEIYRVRVAVPVHLYDTFDYSVSQQQYEQAQAGARVLVSFGRQSLIGVITE
CCCCCCCCCCEEEEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHH
KLAPDLPIDPRFQLKTIRELIDQAPILDTKVLTLLTWSAHYYQFPIGEVIQTALPSLLRQ
HHCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHEECCCEEECCHHHHHHHHHHHHHHC
GKSQDVLTYIWKITAHPEAESKLKRSQKQYDAYQILKLHPTGTTETILNLSGVETATLRA
CCCCCCEEEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCHHHHEECCCCCHHHHHH
LAKKELVACTQEIQDFTPMPVQLAQMPLTPNPDQHNAIQRVLKSLHHYQAFLLDGLTGSG
HHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
KTEVYLQIMHEVLKQGKQVLVLVPEIGLTPQTISRFKSRFHCDVALLHSGLNDSKRLQAW
CHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHH
QQAKMGKASIILGTRSAIYTPLPRLGLIILDEEHDLSYKQQEGFRYHARDVALYRGYLEQ
HHHCCCCEEEEEECCHHHHCCCCCCCEEEEECCCCCCCHHHCCCEEHHHHHHHHHHHHHH
CPVILGSATPSIDSYHLVEQNKLELLQLNQRAGVALLPKMHLIDLKVAKKNEGLSQILID
CCEEEECCCCCCCCEEECCCCCHHHHEECCCCCEEECCCEEEEEEEEECCCCCHHHHHHH
QIQHTLDRQEQVLIFLNRRGYAPVLVCESCGWQANCPNCDAHFTLHTQPYGYLHCHHCGT
HHHHHHCCCCEEEEEEECCCCCCEEEECCCCCCCCCCCCCCEEEEECCCCCEEEEECCCC
IHRLPETCPECHHHTLKTLGMGTAKVEEHLKTLFPEHDVIRVDRDSTSRVGSWQKIYDRI
HHHCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHH
QHNKPMILLGTQMLAKGHHFPYVTLVAILDIDAGLLSVDVRAPERTAQLIVQVAGRAGRG
HCCCCEEEEEHHHHHCCCCCCHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCCC
EHKGHVYMQTLRPDHPMLTTLIEKDYRAVAKDMLEERKAARLPPYRYSVLIRCESRDRDY
CCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCHH
SQAFLQEIAQMLQHQAGDSIEIWGPIPAPMERKAGRYRAHMVILAQDRARLHFFLRPWWA
HHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHCCCCEEEEEEEEECCCCEEEEEECHHHH
EVIQLPRQHQLRLSIDVDPQEFN
HHHHCCCCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2162050; 2162049; 8346018; 9278503; 1662369 [H]