Click here to switch to the map view.

The map label for this gene is kef [H]

Identifier: 50083664

GI number: 50083664

Start: 409071

End: 410915

Strand: Reverse

Name: kef [H]

Synonym: ACIAD0408

Alternate gene names: 50083664

Gene position: 410915-409071 (Counterclockwise)

Preceding gene: 50083665

Following gene: 50083663

Centisome position: 11.42

GC content: 42.66

Gene sequence:

>1845_bases
ATGTCCTTACTGTTACAAATCACCATTTTCCTAGGTGCTGCCCTTCTTCTTGTACCGCTGGGTAAACACTTGGGTATTGC
GACTGTCCTTGGTTATTTGTTTACAGGTATCCTGCTAGGACCCAGTATTTTAAATATTGCGAGTGATGCCAATGCCATTT
TACATATGGCCGATTTTGGCGTGATTTTATTGATGTTTCTGATTGGACTGGAATTGCAGCCACAACGACTGTGGCAGTTA
CGTCATTCCATTTTTGTGATGGGCAGCTTACAAGTGGTGCTGACAGGCATTGTGCTGATAGCCGTGATTATTTTGCTATT
CAAGCTGGATATTGCCAGTGGTTTTGTACTCGGTTTTGCACTGGCACTCTCCTCAACTGCCTTTGTTTTACAACTGCTTG
CAGAAAAACAGCAACTGAGTACCACGTATGGTCAGCAGTCTTTTTCAATTTTGCTGTTTCAGGATATTGCAGCGATTCCG
CTACTGGCCATGATTCCATTATTAACTGGAACCTCATCTACACACCATGGTATTGCCTATTTCGCTGCGATTATTGCGAC
ATTTACAGGGCTATTTTTATTTAGCCGCTATATCATGCGGCCATTTTTTCGTTTTGTTGCCAAAAGTGGTGCAACAGAAC
TGATCACTGCGGTTGGGCTATTTATTGTGCTGGGTGTGGTGATGCTGATGGACTTATTAGGAATCAGCACAACTCTTGGT
GCATTTTTAACAGGCGTTCTTCTCGCAGATTCAGAATTTAGACACGAACTTGAAGCAAGTATCGCGCCATTCAAAGGCCT
TTTACTTGGTCTATTTTTCATGACCGTGGGCATGACCACTCAAGTTTCACTTCTCCTTGATATGCCTTGGCTGATTATTG
GTGGTGCGCTGGGTCTAATTCTGATCAAAGGCATCGTGCTGACCGCAATCTCGCGCTATTTTAAATATAGCTGGCAAAAC
AGTCTGCTGCTGGCTGCCTGCTTGGCACAGGGCGGTGAATTTGCCTTTGTGATTCTGAAAGTAGCGCAAAAAGAAGGGGT
TCTTGCTCAAGCGATGACGGATCCAGTGATTTTGATTGTCACTGTGTCTATGGTCATGACACCTATATGCTATTGGCTTA
TTCAGAAACAGATTCTGCCTTTGTTTTCTAAGGAACATCCACCTGAGTATGATGCTATTCCACAGCATGAACATCCCATG
ATTATCGCGGGTTTTGGTCGTTTTGGTCAGATCATTGCCCGGAATGCACGTCTCAATCATATTGGGTTTACTGCCATTGA
CAATAATCTCCAGCAAATCGATTTTGTCAGACGTTACGGTGGCACATTATACTATGGTGACATCACCCAACCCGATATTT
TACGTGCCGCAGGCATTGAAAAAGCAAAGGTTTTTATTTTAGCGATTGATGATGTCGAAGATAGTATGAATGTCGCACGT
CACATTCGTCTCAATTATCCTCAAATCACGCTGATGGTCAGAGCAAGAGATCGTTATCAGGTTCATCTATTACGTGATCT
TGGGGTTGAGCATATCTGGCGTGAAACTTATCTCTCGTCGTTAGGTATGGCCTATCGTGCTTTATGCGAATTAGGTATTC
CAGAACAACAAGCCTATGACAATATCGAACTGTTTAGAGACTATGATGAAAAGTTGTTGACCCAGCAGCAAAATATTTAC
AATGATGAGCAGAAAGTTTTTGAGACGCATCGAAATGCACTGGCAGAACTCGAACATTTGTTTGAAAGTGATGAACGTCA
TCAAACTAAACGTAATTCTGAGGTTAATTTAAAACGCGGACTGGAAAATCATCGGATTGATGTTACACGAGATGATCCTT
TATGA

Upstream 100 bases:

>100_bases
GATGTTGATCCACAGGAATTTAACTGAAATTTAAACATCATGGGTGGATAATGCTAAAGTTTTATCCACTGCTATATGAT
TGATTGCCTCAGAGTCGCCC

Downstream 100 bases:

>100_bases
CTTGTGTTTTACAATATTGACACCATTAAGACAGGGTTATGGAGAAAAATATGTCTGACCTACGCCAACGCTTTTATATT
GAGGACTGCCCTGTTCGTGG

Product: glutathione-regulated potassium-efflux system protein

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: K(+)/H(+) antiporter; NEM-activatable K(+)/H(+) antiporter [H]

Number of amino acids: Translated: 614; Mature: 613

Protein sequence:

>614_residues
MSLLLQITIFLGAALLLVPLGKHLGIATVLGYLFTGILLGPSILNIASDANAILHMADFGVILLMFLIGLELQPQRLWQL
RHSIFVMGSLQVVLTGIVLIAVIILLFKLDIASGFVLGFALALSSTAFVLQLLAEKQQLSTTYGQQSFSILLFQDIAAIP
LLAMIPLLTGTSSTHHGIAYFAAIIATFTGLFLFSRYIMRPFFRFVAKSGATELITAVGLFIVLGVVMLMDLLGISTTLG
AFLTGVLLADSEFRHELEASIAPFKGLLLGLFFMTVGMTTQVSLLLDMPWLIIGGALGLILIKGIVLTAISRYFKYSWQN
SLLLAACLAQGGEFAFVILKVAQKEGVLAQAMTDPVILIVTVSMVMTPICYWLIQKQILPLFSKEHPPEYDAIPQHEHPM
IIAGFGRFGQIIARNARLNHIGFTAIDNNLQQIDFVRRYGGTLYYGDITQPDILRAAGIEKAKVFILAIDDVEDSMNVAR
HIRLNYPQITLMVRARDRYQVHLLRDLGVEHIWRETYLSSLGMAYRALCELGIPEQQAYDNIELFRDYDEKLLTQQQNIY
NDEQKVFETHRNALAELEHLFESDERHQTKRNSEVNLKRGLENHRIDVTRDDPL

Sequences:

>Translated_614_residues
MSLLLQITIFLGAALLLVPLGKHLGIATVLGYLFTGILLGPSILNIASDANAILHMADFGVILLMFLIGLELQPQRLWQL
RHSIFVMGSLQVVLTGIVLIAVIILLFKLDIASGFVLGFALALSSTAFVLQLLAEKQQLSTTYGQQSFSILLFQDIAAIP
LLAMIPLLTGTSSTHHGIAYFAAIIATFTGLFLFSRYIMRPFFRFVAKSGATELITAVGLFIVLGVVMLMDLLGISTTLG
AFLTGVLLADSEFRHELEASIAPFKGLLLGLFFMTVGMTTQVSLLLDMPWLIIGGALGLILIKGIVLTAISRYFKYSWQN
SLLLAACLAQGGEFAFVILKVAQKEGVLAQAMTDPVILIVTVSMVMTPICYWLIQKQILPLFSKEHPPEYDAIPQHEHPM
IIAGFGRFGQIIARNARLNHIGFTAIDNNLQQIDFVRRYGGTLYYGDITQPDILRAAGIEKAKVFILAIDDVEDSMNVAR
HIRLNYPQITLMVRARDRYQVHLLRDLGVEHIWRETYLSSLGMAYRALCELGIPEQQAYDNIELFRDYDEKLLTQQQNIY
NDEQKVFETHRNALAELEHLFESDERHQTKRNSEVNLKRGLENHRIDVTRDDPL
>Mature_613_residues
SLLLQITIFLGAALLLVPLGKHLGIATVLGYLFTGILLGPSILNIASDANAILHMADFGVILLMFLIGLELQPQRLWQLR
HSIFVMGSLQVVLTGIVLIAVIILLFKLDIASGFVLGFALALSSTAFVLQLLAEKQQLSTTYGQQSFSILLFQDIAAIPL
LAMIPLLTGTSSTHHGIAYFAAIIATFTGLFLFSRYIMRPFFRFVAKSGATELITAVGLFIVLGVVMLMDLLGISTTLGA
FLTGVLLADSEFRHELEASIAPFKGLLLGLFFMTVGMTTQVSLLLDMPWLIIGGALGLILIKGIVLTAISRYFKYSWQNS
LLLAACLAQGGEFAFVILKVAQKEGVLAQAMTDPVILIVTVSMVMTPICYWLIQKQILPLFSKEHPPEYDAIPQHEHPMI
IAGFGRFGQIIARNARLNHIGFTAIDNNLQQIDFVRRYGGTLYYGDITQPDILRAAGIEKAKVFILAIDDVEDSMNVARH
IRLNYPQITLMVRARDRYQVHLLRDLGVEHIWRETYLSSLGMAYRALCELGIPEQQAYDNIELFRDYDEKLLTQQQNIYN
DEQKVFETHRNALAELEHLFESDERHQTKRNSEVNLKRGLENHRIDVTRDDPL

Specific function: Transport system that facilitates potassium-efflux, possibly by potassium-proton antiport [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RCK N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI157388921, Length=376, Percent_Identity=26.8617021276596, Blast_Score=94, Evalue=3e-19,
Organism=Escherichia coli, GI1786232, Length=587, Percent_Identity=39.6933560477002, Blast_Score=411, Evalue=1e-116,
Organism=Escherichia coli, GI1789749, Length=594, Percent_Identity=39.5622895622896, Blast_Score=392, Evalue=1e-110,
Organism=Escherichia coli, GI1786685, Length=518, Percent_Identity=26.0617760617761, Blast_Score=154, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR020884
- InterPro:   IPR004771
- InterPro:   IPR016040
- InterPro:   IPR003148 [H]

Pfam domain/function: PF00999 Na_H_Exchanger; PF02254 TrkA_N [H]

EC number: NA

Molecular weight: Translated: 68506; Mature: 68375

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLLLQITIFLGAALLLVPLGKHLGIATVLGYLFTGILLGPSILNIASDANAILHMADFG
CCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
VILLMFLIGLELQPQRLWQLRHSIFVMGSLQVVLTGIVLIAVIILLFKLDIASGFVLGFA
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LALSSTAFVLQLLAEKQQLSTTYGQQSFSILLFQDIAAIPLLAMIPLLTGTSSTHHGIAY
HHHHHHHHHHHHHHHHHHHHHHHCCCHHEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
FAAIIATFTGLFLFSRYIMRPFFRFVAKSGATELITAVGLFIVLGVVMLMDLLGISTTLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFLTGVLLADSEFRHELEASIAPFKGLLLGLFFMTVGMTTQVSLLLDMPWLIIGGALGLI
HHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHHHHHHH
LIKGIVLTAISRYFKYSWQNSLLLAACLAQGGEFAFVILKVAQKEGVLAQAMTDPVILIV
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEHEEHHHHCCHHHHHHCCCCHHHH
TVSMVMTPICYWLIQKQILPLFSKEHPPEYDAIPQHEHPMIIAGFGRFGQIIARNARLNH
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHCCCCCCC
IGFTAIDNNLQQIDFVRRYGGTLYYGDITQPDILRAAGIEKAKVFILAIDDVEDSMNVAR
CCCHHHCCCHHHHHHHHHHCCEEEECCCCCCHHHHHCCCCEEEEEEEEECCCHHHHHHHH
HIRLNYPQITLMVRARDRYQVHLLRDLGVEHIWRETYLSSLGMAYRALCELGIPEQQAYD
HHEECCCEEEEEEEECCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHC
NIELFRDYDEKLLTQQQNIYNDEQKVFETHRNALAELEHLFESDERHQTKRNSEVNLKRG
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHC
LENHRIDVTRDDPL
CCCCCEECCCCCCC
>Mature Secondary Structure 
SLLLQITIFLGAALLLVPLGKHLGIATVLGYLFTGILLGPSILNIASDANAILHMADFG
CHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
VILLMFLIGLELQPQRLWQLRHSIFVMGSLQVVLTGIVLIAVIILLFKLDIASGFVLGFA
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LALSSTAFVLQLLAEKQQLSTTYGQQSFSILLFQDIAAIPLLAMIPLLTGTSSTHHGIAY
HHHHHHHHHHHHHHHHHHHHHHHCCCHHEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
FAAIIATFTGLFLFSRYIMRPFFRFVAKSGATELITAVGLFIVLGVVMLMDLLGISTTLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFLTGVLLADSEFRHELEASIAPFKGLLLGLFFMTVGMTTQVSLLLDMPWLIIGGALGLI
HHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHHHHHHH
LIKGIVLTAISRYFKYSWQNSLLLAACLAQGGEFAFVILKVAQKEGVLAQAMTDPVILIV
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEHEEHHHHCCHHHHHHCCCCHHHH
TVSMVMTPICYWLIQKQILPLFSKEHPPEYDAIPQHEHPMIIAGFGRFGQIIARNARLNH
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHCCCCCCC
IGFTAIDNNLQQIDFVRRYGGTLYYGDITQPDILRAAGIEKAKVFILAIDDVEDSMNVAR
CCCHHHCCCHHHHHHHHHHCCEEEECCCCCCHHHHHCCCCEEEEEEEEECCCHHHHHHHH
HIRLNYPQITLMVRARDRYQVHLLRDLGVEHIWRETYLSSLGMAYRALCELGIPEQQAYD
HHEECCCEEEEEEEECCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHC
NIELFRDYDEKLLTQQQNIYNDEQKVFETHRNALAELEHLFESDERHQTKRNSEVNLKRG
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHC
LENHRIDVTRDDPL
CCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA