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The map label for this gene is pilB [H]

Identifier: 50083624

GI number: 50083624

Start: 354628

End: 356367

Strand: Reverse

Name: pilB [H]

Synonym: ACIAD0362

Alternate gene names: 50083624

Gene position: 356367-354628 (Counterclockwise)

Preceding gene: 50083631

Following gene: 50083623

Centisome position: 9.9

GC content: 41.09

Gene sequence:

>1740_bases
ATGATATCAGAATCACAAGGCGGTCTAATGTCAGCATTTACAACACCACCTAAGTTTTCGGGGTTTATTCGGCGTTTAGT
CGAAGAAGGCTATGTGAATGCACAAAATATGCAGCAGGCTTTGGAAAAAGCAAAAAAATTTAAACAAGATATTGTGCCCT
ACTTGATTGATAATTTTTCAATCTCTCCACTCACCATTGCAGAAATCATTTCTTTAGAGTTTGGAGAACCATTACTTGAT
CTGGGTGTTTTTGATCCCGCTTTATTTTTAAAAGATAAAATTGATGAAAAACTGATCCAGAAATATCGAATCATGCCATT
GGTGCATCGCGGGCATGTGTTGTATGTCGCAACCAGTAATCCAACCAACATTGAGGCAATGGATGCGATTCGATTTAATA
GCAAGCTCAAAGTTGAACCAATTATTGTTGAACATGACAAATTAGAGCGATTGCTTTCAGAACATTTTGTTGAGGAAACT
CATTTTAATTTTGATACAGAAGAGCTTGATCTGGATGTTGAAGTCGATCCACATACAACGGATGACGACGATGAGGATGA
CAAACTTAAAGACGAAGCACCTATTGTTAAATACATTAATAAGTTACTGATTGATGCGATACGCATGAGTGCTTCGGATT
TACACTTTGAGCCCTATGAGAAAAGCTATCGGGTCCGTTATCGGGTAGATGGTGTACTGCGGTTGATTGCAACACCTCCC
CTGCAACTGGCAACACGACTGGCCTCACGTTTGAAAGTCATGTCACAAATGGATATTTCGGAAAAACGTGTTCCGCAAGA
TGGCCGTATTAAACTCAAAATGTCTAAAAGTAAAACCATTGATTTTCGGGTCAATTCTTTGCCGACATTATTTGGGGAAA
AAATTGTTCTTCGGATTCTTGATCCAGCCAGTGCCATGCTCGGTATTGATGCACTCGGTTATGAACCCGAGCAAAAAGCA
CTATTTATGGAGGCTTTAAACAAACCGCAAGGGATGCTTCTAATTACGGGACCAACGGGTTCAGGTAAAACCGTATCTTT
ATATACAGGCTTAAATATACTCAATACCGAACATGCCAATATTTCCACAGCAGAAGATCCAGTAGAAATCAATCTCGAAG
GCGTCAATCAGGTCAATGTCAATCCAAAGGTTGGACTGACATTTGCTGCTGCATTAAGATCCTTTTTACGTCAGGATCCT
GATATTATTATGGTCGGGGAAATACGTGATCTGGAAACCGCTGAGATCGCCATTAAAGCAGCTCAAACAGGTCATTTGGT
CATGTCGACGTTGCATACCAATAATGCAGCAGAAACACTGACTCGACTCAGAAATATGGGCGTTGCTTCTTTTAATATTG
CAACCTCAGTGAATCTGGTGATCGCACAACGTCTTGCTAGGCGTTTATGCAGCCAGTGCAAACGACCGATTCAGGTTCCA
GAGCGAAGTTTGCTGGAAATGGGCTTTACGCCAGAAGATTTAGCTCAACCAGAATTTCAGATTTTTGAGCCTGTAGGGTG
TCATGACTGTCGCGAAGGCTATAAAGGCCGTGTGGGGATTTACGAAGTCATGAAAATTACACCAGAAATATCAAAAATTA
TTATGGAAGATGGTAATGCGCTAGAAATTGCCGCAACAGCCGAAACATTGGGGTTCAATAATTTACGTCGGTCGGGTCTG
AAAAAAGTGATGCAGGGGGTCACCTCGTTACAGGAAATTAATCGTGTAACCAGTGAATAA

Upstream 100 bases:

>100_bases
ACGTCAAGTAACAAATTAACCTCTCCGATGTATCGAAAGTCATGTCGCAAGGGTAGAATTTTTCCATAAGCCCCGTATAA
TTTTCTATATACAAATTATA

Downstream 100 bases:

>100_bases
TGCAAAGTCTCAAAATAAGGATAACCAAATGGCAGCAAAAAAAACGCAAGTAATGCCAGTATTTGCTTATGAAGGTGTCG
ACCGTAAAGGCATCAAGTTA

Product: type 4 fimbrial biogenesis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 579; Mature: 579

Protein sequence:

>579_residues
MISESQGGLMSAFTTPPKFSGFIRRLVEEGYVNAQNMQQALEKAKKFKQDIVPYLIDNFSISPLTIAEIISLEFGEPLLD
LGVFDPALFLKDKIDEKLIQKYRIMPLVHRGHVLYVATSNPTNIEAMDAIRFNSKLKVEPIIVEHDKLERLLSEHFVEET
HFNFDTEELDLDVEVDPHTTDDDDEDDKLKDEAPIVKYINKLLIDAIRMSASDLHFEPYEKSYRVRYRVDGVLRLIATPP
LQLATRLASRLKVMSQMDISEKRVPQDGRIKLKMSKSKTIDFRVNSLPTLFGEKIVLRILDPASAMLGIDALGYEPEQKA
LFMEALNKPQGMLLITGPTGSGKTVSLYTGLNILNTEHANISTAEDPVEINLEGVNQVNVNPKVGLTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHLVMSTLHTNNAAETLTRLRNMGVASFNIATSVNLVIAQRLARRLCSQCKRPIQVP
ERSLLEMGFTPEDLAQPEFQIFEPVGCHDCREGYKGRVGIYEVMKITPEISKIIMEDGNALEIAATAETLGFNNLRRSGL
KKVMQGVTSLQEINRVTSE

Sequences:

>Translated_579_residues
MISESQGGLMSAFTTPPKFSGFIRRLVEEGYVNAQNMQQALEKAKKFKQDIVPYLIDNFSISPLTIAEIISLEFGEPLLD
LGVFDPALFLKDKIDEKLIQKYRIMPLVHRGHVLYVATSNPTNIEAMDAIRFNSKLKVEPIIVEHDKLERLLSEHFVEET
HFNFDTEELDLDVEVDPHTTDDDDEDDKLKDEAPIVKYINKLLIDAIRMSASDLHFEPYEKSYRVRYRVDGVLRLIATPP
LQLATRLASRLKVMSQMDISEKRVPQDGRIKLKMSKSKTIDFRVNSLPTLFGEKIVLRILDPASAMLGIDALGYEPEQKA
LFMEALNKPQGMLLITGPTGSGKTVSLYTGLNILNTEHANISTAEDPVEINLEGVNQVNVNPKVGLTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHLVMSTLHTNNAAETLTRLRNMGVASFNIATSVNLVIAQRLARRLCSQCKRPIQVP
ERSLLEMGFTPEDLAQPEFQIFEPVGCHDCREGYKGRVGIYEVMKITPEISKIIMEDGNALEIAATAETLGFNNLRRSGL
KKVMQGVTSLQEINRVTSE
>Mature_579_residues
MISESQGGLMSAFTTPPKFSGFIRRLVEEGYVNAQNMQQALEKAKKFKQDIVPYLIDNFSISPLTIAEIISLEFGEPLLD
LGVFDPALFLKDKIDEKLIQKYRIMPLVHRGHVLYVATSNPTNIEAMDAIRFNSKLKVEPIIVEHDKLERLLSEHFVEET
HFNFDTEELDLDVEVDPHTTDDDDEDDKLKDEAPIVKYINKLLIDAIRMSASDLHFEPYEKSYRVRYRVDGVLRLIATPP
LQLATRLASRLKVMSQMDISEKRVPQDGRIKLKMSKSKTIDFRVNSLPTLFGEKIVLRILDPASAMLGIDALGYEPEQKA
LFMEALNKPQGMLLITGPTGSGKTVSLYTGLNILNTEHANISTAEDPVEINLEGVNQVNVNPKVGLTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHLVMSTLHTNNAAETLTRLRNMGVASFNIATSVNLVIAQRLARRLCSQCKRPIQVP
ERSLLEMGFTPEDLAQPEFQIFEPVGCHDCREGYKGRVGIYEVMKITPEISKIIMEDGNALEIAATAETLGFNNLRRSGL
KKVMQGVTSLQEINRVTSE

Specific function: Involved in the translocation of the type IV pilin (pilA) [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=422, Percent_Identity=46.6824644549763, Blast_Score=361, Evalue=1e-101,
Organism=Escherichia coli, GI1786296, Length=468, Percent_Identity=39.957264957265, Blast_Score=349, Evalue=2e-97,
Organism=Escherichia coli, GI87082188, Length=276, Percent_Identity=31.1594202898551, Blast_Score=111, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013374
- InterPro:   IPR007831
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 64881; Mature: 64881

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISESQGGLMSAFTTPPKFSGFIRRLVEEGYVNAQNMQQALEKAKKFKQDIVPYLIDNFS
CCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
ISPLTIAEIISLEFGEPLLDLGVFDPALFLKDKIDEKLIQKYRIMPLVHRGHVLYVATSN
CCCCHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCEEEECCEEEEEEECC
PTNIEAMDAIRFNSKLKVEPIIVEHDKLERLLSEHFVEETHFNFDTEELDLDVEVDPHTT
CCCCCHHHHEECCCCEEEEEEEECHHHHHHHHHHHHHHHHCCCCCHHHEEEEEEECCCCC
DDDDEDDKLKDEAPIVKYINKLLIDAIRMSASDLHFEPYEKSYRVRYRVDGVLRLIATPP
CCCCCCCHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEHHHHHHHHCCCC
LQLATRLASRLKVMSQMDISEKRVPQDGRIKLKMSKSKTIDFRVNSLPTLFGEKIVLRIL
HHHHHHHHHHHHHHHHCCCHHHCCCCCCCEEEEECCCCEEEEEECCCCHHHHCCEEEEEE
DPASAMLGIDALGYEPEQKALFMEALNKPQGMLLITGPTGSGKTVSLYTGLNILNTEHAN
CCHHHHHCCHHCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEECCEEEECCCCC
ISTAEDPVEINLEGVNQVNVNPKVGLTFAAALRSFLRQDPDIIMVGEIRDLETAEIAIKA
CCCCCCCEEEEEECCEEECCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHEEEEE
AQTGHLVMSTLHTNNAAETLTRLRNMGVASFNIATSVNLVIAQRLARRLCSQCKRPIQVP
CCCCCEEEEEHHCCCHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCC
ERSLLEMGFTPEDLAQPEFQIFEPVGCHDCREGYKGRVGIYEVMKITPEISKIIMEDGNA
CHHHHHCCCCHHHHCCCCCEEECCCCCHHHHHCCCCCCCHHHHHHHCHHHHHHHHCCCCE
LEIAATAETLGFNNLRRSGLKKVMQGVTSLQEINRVTSE
EEEEEEHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MISESQGGLMSAFTTPPKFSGFIRRLVEEGYVNAQNMQQALEKAKKFKQDIVPYLIDNFS
CCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
ISPLTIAEIISLEFGEPLLDLGVFDPALFLKDKIDEKLIQKYRIMPLVHRGHVLYVATSN
CCCCHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCEEEECCEEEEEEECC
PTNIEAMDAIRFNSKLKVEPIIVEHDKLERLLSEHFVEETHFNFDTEELDLDVEVDPHTT
CCCCCHHHHEECCCCEEEEEEEECHHHHHHHHHHHHHHHHCCCCCHHHEEEEEEECCCCC
DDDDEDDKLKDEAPIVKYINKLLIDAIRMSASDLHFEPYEKSYRVRYRVDGVLRLIATPP
CCCCCCCHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEHHHHHHHHCCCC
LQLATRLASRLKVMSQMDISEKRVPQDGRIKLKMSKSKTIDFRVNSLPTLFGEKIVLRIL
HHHHHHHHHHHHHHHHCCCHHHCCCCCCCEEEEECCCCEEEEEECCCCHHHHCCEEEEEE
DPASAMLGIDALGYEPEQKALFMEALNKPQGMLLITGPTGSGKTVSLYTGLNILNTEHAN
CCHHHHHCCHHCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEECCEEEECCCCC
ISTAEDPVEINLEGVNQVNVNPKVGLTFAAALRSFLRQDPDIIMVGEIRDLETAEIAIKA
CCCCCCCEEEEEECCEEECCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHEEEEE
AQTGHLVMSTLHTNNAAETLTRLRNMGVASFNIATSVNLVIAQRLARRLCSQCKRPIQVP
CCCCCEEEEEHHCCCHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCC
ERSLLEMGFTPEDLAQPEFQIFEPVGCHDCREGYKGRVGIYEVMKITPEISKIIMEDGNA
CHHHHHCCCCHHHHCCCCCEEECCCCCHHHHHCCCCCCCHHHHHHHCHHHHHHHHCCCCE
LEIAATAETLGFNNLRRSGLKKVMQGVTSLQEINRVTSE
EEEEEEHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1971619; 10984043 [H]