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The map label for this gene is trpE [H]

Identifier: 50083571

GI number: 50083571

Start: 295163

End: 296656

Strand: Direct

Name: trpE [H]

Synonym: ACIAD0297

Alternate gene names: 50083571

Gene position: 295163-296656 (Clockwise)

Preceding gene: 50083570

Following gene: 50083572

Centisome position: 8.2

GC content: 45.18

Gene sequence:

>1494_bases
ATGACATCACTAACTCAATTTGAACAGCTTAAAACAGCAGGCTATAACACCATTCCTGTTTATCGTCAGCGTCTTGCTGA
TACCGATACACCATTGTCCGTCTTTGCTCGACTCAAGGACTATACACAAGCCTATTTGTTTGAGTCGGTTGAGGGTGGAG
AGAACTGGGCGCGCTATTCCATTATTGGCTTGGGTGAGTCTACCGTTTTTTCTTGTAATGCGGGGCAGCTCACGATCAAA
AATGCACAAGGGAACATTACAACACAATCTTGTGCAGATCCATTTCAGTATATTCGTGATTATCAAAGCCAGTTTAAAGT
TCCACCGCATGCGCTTATACCAGACTTGCCTCAATTTACTGGGGGGCTGGTCGGTTATTTTGGCTACGATGCGGTGCGTT
ATATCGAACCGCGTTTGAGTAACGTTCCAGAGGCAGATCCAGTCGGTTTACCTGACATCTGGATGATGCTCTCTAAGACC
GTGATTATTTTTGATAATCTCAAAGACACGCTGTTTTTGATTGTGCATGCCAATACCACTGATGAAGAAGCCTATCAAGC
TGCGCAGAATCAACTGGATTATCTGGAACGATTGCTGGCAACACCAGTGAGCTTGCAGGCCAAAAAACATAAAGCACCAC
ATTTTGAATCCCTTACAGGGAAAGAAAAATTCTTAGAATCGGTTGAAACCGTCAAGGAATATATTCGTGCGGGCGATGTC
ATGCAAGTGGTTCCAGGGCACCGTATGGTCTCTGATTTTGATGGCGATCCTTTACAGGTCTATCGTGCATTGCGCCATTT
AAACCCTTCGCCTTATCTGTTTTTAGTGCAGGGACACACACTCACGGATAACACGCCTTTTCACATTGTTGGCTCATCAC
CAGAAATCCTGTCACGCTTGGAAGATGGTATTGCCACAGTTAGACCTCTTGCGGGAACTCGCCCTCGTGGTAAGACCAAA
GAGGAAGATTTGGCGCTCGAACAAGAGCTGCTCGCTGATGAAAAAGAAATTGCAGAGCATTTAATGCTGATCGATCTTGG
ACGTAACGATGTGGGGCGTGTCTCAAAAATTGGAAAGGTGCAGGTGACCGATCGAATGGTGATCGAACGTTATTCACATG
TCATGCATATTGTCTCCAATGTACAAGGCGAAGTGCGGGATGATATTGATGCATTGGACGTATTTAAAGCAACCTTTCCT
GCGGGTACGCTTTCTGGGGCACCCAAAATTCGTGCCATGGAAATCATTGATGAAGTTGAACCAGTCAAGCGTGGAATTTT
TGGTGGTGCTGTCGGATATTTAGGCTGGCATGGCGAAATGGATATGTCGATTGCCATTCGTACTTGTGTGATTCGTGAAA
ATAAAGTCTATGTCCAGGCAGGAGCAGGGCTGGTGGCGGACTCAAATCCAGAATCTGAATGGAATGAAACCCAAATAAAA
GCTCGCGCAGTGATCAAAGCGGTTGAATTATCATCAAATGGATTGATTTTATGA

Upstream 100 bases:

>100_bases
TTGAGGAGTGGGGAATGTATCACCTTATGGTTTTTCGATGGCGATAATCGCGTCTTAAATCACCTTTATCGTACTTAACT
TAAAACCATCGAGAATACTC

Downstream 100 bases:

>100_bases
GTTTTTAGCGGTTTTTTTAAAAAAACCGCTTGCATCGTTTCTGAGTTTTGCTAAACTGCACACCGTTCCGATACGAAACG
TACGAAACACTAAGAAACGC

Product: anthranilate synthase component I

Products: NA

Alternate protein names: Anthranilate synthase component I [H]

Number of amino acids: Translated: 497; Mature: 496

Protein sequence:

>497_residues
MTSLTQFEQLKTAGYNTIPVYRQRLADTDTPLSVFARLKDYTQAYLFESVEGGENWARYSIIGLGESTVFSCNAGQLTIK
NAQGNITTQSCADPFQYIRDYQSQFKVPPHALIPDLPQFTGGLVGYFGYDAVRYIEPRLSNVPEADPVGLPDIWMMLSKT
VIIFDNLKDTLFLIVHANTTDEEAYQAAQNQLDYLERLLATPVSLQAKKHKAPHFESLTGKEKFLESVETVKEYIRAGDV
MQVVPGHRMVSDFDGDPLQVYRALRHLNPSPYLFLVQGHTLTDNTPFHIVGSSPEILSRLEDGIATVRPLAGTRPRGKTK
EEDLALEQELLADEKEIAEHLMLIDLGRNDVGRVSKIGKVQVTDRMVIERYSHVMHIVSNVQGEVRDDIDALDVFKATFP
AGTLSGAPKIRAMEIIDEVEPVKRGIFGGAVGYLGWHGEMDMSIAIRTCVIRENKVYVQAGAGLVADSNPESEWNETQIK
ARAVIKAVELSSNGLIL

Sequences:

>Translated_497_residues
MTSLTQFEQLKTAGYNTIPVYRQRLADTDTPLSVFARLKDYTQAYLFESVEGGENWARYSIIGLGESTVFSCNAGQLTIK
NAQGNITTQSCADPFQYIRDYQSQFKVPPHALIPDLPQFTGGLVGYFGYDAVRYIEPRLSNVPEADPVGLPDIWMMLSKT
VIIFDNLKDTLFLIVHANTTDEEAYQAAQNQLDYLERLLATPVSLQAKKHKAPHFESLTGKEKFLESVETVKEYIRAGDV
MQVVPGHRMVSDFDGDPLQVYRALRHLNPSPYLFLVQGHTLTDNTPFHIVGSSPEILSRLEDGIATVRPLAGTRPRGKTK
EEDLALEQELLADEKEIAEHLMLIDLGRNDVGRVSKIGKVQVTDRMVIERYSHVMHIVSNVQGEVRDDIDALDVFKATFP
AGTLSGAPKIRAMEIIDEVEPVKRGIFGGAVGYLGWHGEMDMSIAIRTCVIRENKVYVQAGAGLVADSNPESEWNETQIK
ARAVIKAVELSSNGLIL
>Mature_496_residues
TSLTQFEQLKTAGYNTIPVYRQRLADTDTPLSVFARLKDYTQAYLFESVEGGENWARYSIIGLGESTVFSCNAGQLTIKN
AQGNITTQSCADPFQYIRDYQSQFKVPPHALIPDLPQFTGGLVGYFGYDAVRYIEPRLSNVPEADPVGLPDIWMMLSKTV
IIFDNLKDTLFLIVHANTTDEEAYQAAQNQLDYLERLLATPVSLQAKKHKAPHFESLTGKEKFLESVETVKEYIRAGDVM
QVVPGHRMVSDFDGDPLQVYRALRHLNPSPYLFLVQGHTLTDNTPFHIVGSSPEILSRLEDGIATVRPLAGTRPRGKTKE
EDLALEQELLADEKEIAEHLMLIDLGRNDVGRVSKIGKVQVTDRMVIERYSHVMHIVSNVQGEVRDDIDALDVFKATFPA
GTLSGAPKIRAMEIIDEVEPVKRGIFGGAVGYLGWHGEMDMSIAIRTCVIRENKVYVQAGAGLVADSNPESEWNETQIKA
RAVIKAVELSSNGLIL

Specific function: Tryptophan biosynthesis; first step. [C]

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anthranilate synthase component I family [H]

Homologues:

Organism=Escherichia coli, GI1787518, Length=386, Percent_Identity=40.1554404145078, Blast_Score=245, Evalue=4e-66,
Organism=Escherichia coli, GI1788114, Length=459, Percent_Identity=32.6797385620915, Blast_Score=225, Evalue=6e-60,
Organism=Escherichia coli, GI1786809, Length=202, Percent_Identity=31.1881188118812, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87082077, Length=197, Percent_Identity=28.4263959390863, Blast_Score=74, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320935, Length=508, Percent_Identity=37.4015748031496, Blast_Score=298, Evalue=1e-81,
Organism=Saccharomyces cerevisiae, GI6324361, Length=379, Percent_Identity=25.3298153034301, Blast_Score=105, Evalue=2e-23,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR019999
- InterPro:   IPR006805
- InterPro:   IPR005256
- InterPro:   IPR015890 [H]

Pfam domain/function: PF04715 Anth_synt_I_N; PF00425 Chorismate_bind [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 55302; Mature: 55170

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSLTQFEQLKTAGYNTIPVYRQRLADTDTPLSVFARLKDYTQAYLFESVEGGENWARYS
CCCHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
IIGLGESTVFSCNAGQLTIKNAQGNITTQSCADPFQYIRDYQSQFKVPPHALIPDLPQFT
EEECCCCEEEECCCCEEEEECCCCCCCHHHHCCHHHHHHHHHHHCCCCCHHHCCCCHHHH
GGLVGYFGYDAVRYIEPRLSNVPEADPVGLPDIWMMLSKTVIIFDNLKDTLFLIVHANTT
CCHHHHHHHHHHHHHCHHHCCCCCCCCCCCHHHHHHHCCEEEEEECCCCEEEEEEECCCC
DEEAYQAAQNQLDYLERLLATPVSLQAKKHKAPHFESLTGKEKFLESVETVKEYIRAGDV
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCHHCCCHHHHHHHHHHHHHHHHCCCE
MQVVPGHRMVSDFDGDPLQVYRALRHLNPSPYLFLVQGHTLTDNTPFHIVGSSPEILSRL
EEECCCCCEECCCCCCHHHHHHHHHHCCCCCEEEEEECCEECCCCCEEEECCCHHHHHHH
EDGIATVRPLAGTRPRGKTKEEDLALEQELLADEKEIAEHLMLIDLGRNDVGRVSKIGKV
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHEEEECCCCCHHHHHHCCEE
QVTDRMVIERYSHVMHIVSNVQGEVRDDIDALDVFKATFPAGTLSGAPKIRAMEIIDEVE
EHHHHHHHHHHHHHHHHHHHCCCHHHHCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
PVKRGIFGGAVGYLGWHGEMDMSIAIRTCVIRENKVYVQAGAGLVADSNPESEWNETQIK
HHHHHHHCCHHHHCEECCCCCCEEEEEEEEEECCEEEEEECCCEEECCCCCCCCHHHHHH
ARAVIKAVELSSNGLIL
HHHHHHHEEECCCCCCC
>Mature Secondary Structure 
TSLTQFEQLKTAGYNTIPVYRQRLADTDTPLSVFARLKDYTQAYLFESVEGGENWARYS
CCHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
IIGLGESTVFSCNAGQLTIKNAQGNITTQSCADPFQYIRDYQSQFKVPPHALIPDLPQFT
EEECCCCEEEECCCCEEEEECCCCCCCHHHHCCHHHHHHHHHHHCCCCCHHHCCCCHHHH
GGLVGYFGYDAVRYIEPRLSNVPEADPVGLPDIWMMLSKTVIIFDNLKDTLFLIVHANTT
CCHHHHHHHHHHHHHCHHHCCCCCCCCCCCHHHHHHHCCEEEEEECCCCEEEEEEECCCC
DEEAYQAAQNQLDYLERLLATPVSLQAKKHKAPHFESLTGKEKFLESVETVKEYIRAGDV
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCHHCCCHHHHHHHHHHHHHHHHCCCE
MQVVPGHRMVSDFDGDPLQVYRALRHLNPSPYLFLVQGHTLTDNTPFHIVGSSPEILSRL
EEECCCCCEECCCCCCHHHHHHHHHHCCCCCEEEEEECCEECCCCCEEEECCCHHHHHHH
EDGIATVRPLAGTRPRGKTKEEDLALEQELLADEKEIAEHLMLIDLGRNDVGRVSKIGKV
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHEEEECCCCCHHHHHHCCEE
QVTDRMVIERYSHVMHIVSNVQGEVRDDIDALDVFKATFPAGTLSGAPKIRAMEIIDEVE
EHHHHHHHHHHHHHHHHHHHCCCHHHHCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
PVKRGIFGGAVGYLGWHGEMDMSIAIRTCVIRENKVYVQAGAGLVADSNPESEWNETQIK
HHHHHHHCCHHHHCEECCCCCCEEEEEEEEEECCEEEEEECCCEEECCCCCCCCHHHHHH
ARAVIKAVELSSNGLIL
HHHHHHHEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2338941 [H]