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The map label for this gene is pilR [H]

Identifier: 50083536

GI number: 50083536

Start: 257836

End: 259251

Strand: Reverse

Name: pilR [H]

Synonym: ACIAD0258

Alternate gene names: 50083536

Gene position: 259251-257836 (Counterclockwise)

Preceding gene: 50083537

Following gene: 50083531

Centisome position: 7.2

GC content: 41.38

Gene sequence:

>1416_bases
ATGACCGAACAACAACCACGTGTTTTACTCATTGATGATGAAGAAGACCTTTGTACATTGATGCAAATCAGTCTTTCACG
TCTCGGCGTCCAGACAGATTGTGCCTACAACCTACAACGTGCCAAACAATTGTTACAACAACAACATTATGATGCCTGCC
TAACCGATCTCAACCTACCCGATGGACATGGACTTGATTTTGTTCAGTACGTTACAGAACGCTTTCCTTACTTACCCATT
GCCGTGATTACGGCGTATGGCAATATGGGCATTGCAATTGCAGCGTTAAAAGCCGGTGCTTTTGACTTTGTGAGCAAACC
CATCAATCAACAGCATCTTAGTCAACTGATTCAAACTGCACTGCATAGTAGCCAAACGGCACCAGACAATGAACAAGACA
CGCTTGAGCATCGTTTATTGATTGGTCAATCTGCACCCATTGAACAACTACGTATAGCACTTAGAAAAATTGCACGTTCG
CAGGCACCCGTATTTATTACAGGTGAGTCTGGAACAGGTAAGGAAGTGGTTGCCAATCTGGTACATCGCTTAAGCAATCG
CAATGAAGGGCCGTTTATTGCACTAAACTGTGGTGCAATTCCGTCAGAACTCATGGAGAGTGAGTTATTTGGCCATAAGA
AAGGCAGCTTCACAGGTGCCACACAAGATAAACAGGGATTACTTTTATCGGCACATGGAGGAAGTCTATTTCTGGATGAA
ATTGCAGAACTTCCTCTCACGATGCAAGTTAAATTACTACGCGCTGTTCAAGAAAAGAAAATCCGTCCAATTGGTTCAGA
TCGTGAAATTGATGTCGATTTTCGTGTGATCAGTGCCAGCCATCAGAATCTGGAACTTTTAGTCCAGCAAGGCAAATTTC
GTCAGGATTTATTCTTTCGAATTCATGTCATGGATATTCAGCTTCCTCCACTACGTGAGCGTGGACAAGATATTTTACTG
TTAGCCAAGCATTTTATTCAGCATGTTTGTCATGAATGGGACCTGCCTTTAAAGCATTTGAGCCCTGCGGCTGAACATTA
TCTACTACATTATGACTTTCCAGGAAATGTTCGGGAATTACGCAATATGATGGAACGTGCCATTACCTTAAGTGATGGAG
CATCAATAGACCTTCAGCAATTAAACAGCTCTCTACTACGCCAAACCACTATCCCAGAATTAGATTTAACACCGATTTCG
AATACAGTATCAATAGCACCAACATCTCAGCAAAATTTAGAATTTCCACATGAAGGACTTGAACAATATTTAGAAAAAGT
TGAAAAAGATATGCTGCTTAATGCGTTAAATAGTACACACTGGAATCGTACTTTAGCCGCAAAAAAATTAGGAATGAGTT
TTCGCTCTCTTCGCTATCGTCTGAAAAAATTTGGGCTTGATACTGATGATGGCTAA

Upstream 100 bases:

>100_bases
TCAATCATGCCAAGCTAACGTATGTAGAACAAGAAAAAGGAGCCTGTTTTAGAATTCAGTGCTCAATAAAGGAAAACGAA
TAATTTAGGTTTTTGGGGTT

Downstream 100 bases:

>100_bases
ATCAGGATTTGGGCTGTTTAAGCAATAGATCTGCTACATTTTCCAAATAAGTCTCATCTTTCATGTCGGCAGGTAAAGGA
TAACGCTGGTTCGGCTGAAT

Product: type 4 fimbriae expression regulatory protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MTEQQPRVLLIDDEEDLCTLMQISLSRLGVQTDCAYNLQRAKQLLQQQHYDACLTDLNLPDGHGLDFVQYVTERFPYLPI
AVITAYGNMGIAIAALKAGAFDFVSKPINQQHLSQLIQTALHSSQTAPDNEQDTLEHRLLIGQSAPIEQLRIALRKIARS
QAPVFITGESGTGKEVVANLVHRLSNRNEGPFIALNCGAIPSELMESELFGHKKGSFTGATQDKQGLLLSAHGGSLFLDE
IAELPLTMQVKLLRAVQEKKIRPIGSDREIDVDFRVISASHQNLELLVQQGKFRQDLFFRIHVMDIQLPPLRERGQDILL
LAKHFIQHVCHEWDLPLKHLSPAAEHYLLHYDFPGNVRELRNMMERAITLSDGASIDLQQLNSSLLRQTTIPELDLTPIS
NTVSIAPTSQQNLEFPHEGLEQYLEKVEKDMLLNALNSTHWNRTLAAKKLGMSFRSLRYRLKKFGLDTDDG

Sequences:

>Translated_471_residues
MTEQQPRVLLIDDEEDLCTLMQISLSRLGVQTDCAYNLQRAKQLLQQQHYDACLTDLNLPDGHGLDFVQYVTERFPYLPI
AVITAYGNMGIAIAALKAGAFDFVSKPINQQHLSQLIQTALHSSQTAPDNEQDTLEHRLLIGQSAPIEQLRIALRKIARS
QAPVFITGESGTGKEVVANLVHRLSNRNEGPFIALNCGAIPSELMESELFGHKKGSFTGATQDKQGLLLSAHGGSLFLDE
IAELPLTMQVKLLRAVQEKKIRPIGSDREIDVDFRVISASHQNLELLVQQGKFRQDLFFRIHVMDIQLPPLRERGQDILL
LAKHFIQHVCHEWDLPLKHLSPAAEHYLLHYDFPGNVRELRNMMERAITLSDGASIDLQQLNSSLLRQTTIPELDLTPIS
NTVSIAPTSQQNLEFPHEGLEQYLEKVEKDMLLNALNSTHWNRTLAAKKLGMSFRSLRYRLKKFGLDTDDG
>Mature_470_residues
TEQQPRVLLIDDEEDLCTLMQISLSRLGVQTDCAYNLQRAKQLLQQQHYDACLTDLNLPDGHGLDFVQYVTERFPYLPIA
VITAYGNMGIAIAALKAGAFDFVSKPINQQHLSQLIQTALHSSQTAPDNEQDTLEHRLLIGQSAPIEQLRIALRKIARSQ
APVFITGESGTGKEVVANLVHRLSNRNEGPFIALNCGAIPSELMESELFGHKKGSFTGATQDKQGLLLSAHGGSLFLDEI
AELPLTMQVKLLRAVQEKKIRPIGSDREIDVDFRVISASHQNLELLVQQGKFRQDLFFRIHVMDIQLPPLRERGQDILLL
AKHFIQHVCHEWDLPLKHLSPAAEHYLLHYDFPGNVRELRNMMERAITLSDGASIDLQQLNSSLLRQTTIPELDLTPISN
TVSIAPTSQQNLEFPHEGLEQYLEKVEKDMLLNALNSTHWNRTLAAKKLGMSFRSLRYRLKKFGLDTDDG

Specific function: Member of the two-component regulatory system pilS/pilR that regulates the expression of type 4 fimbriae. When activated by pilS it acts in conjunction with sigma-54 as a transcriptional activator [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=472, Percent_Identity=37.0762711864407, Blast_Score=322, Evalue=4e-89,
Organism=Escherichia coli, GI1790437, Length=464, Percent_Identity=36.2068965517241, Blast_Score=311, Evalue=7e-86,
Organism=Escherichia coli, GI1790299, Length=464, Percent_Identity=35.7758620689655, Blast_Score=298, Evalue=4e-82,
Organism=Escherichia coli, GI1788905, Length=376, Percent_Identity=37.7659574468085, Blast_Score=280, Evalue=2e-76,
Organism=Escherichia coli, GI1789233, Length=356, Percent_Identity=41.2921348314607, Blast_Score=252, Evalue=5e-68,
Organism=Escherichia coli, GI1786524, Length=425, Percent_Identity=37.4117647058824, Blast_Score=239, Evalue=3e-64,
Organism=Escherichia coli, GI87082117, Length=327, Percent_Identity=41.2844036697248, Blast_Score=238, Evalue=6e-64,
Organism=Escherichia coli, GI87082152, Length=332, Percent_Identity=39.1566265060241, Blast_Score=219, Evalue=4e-58,
Organism=Escherichia coli, GI1789087, Length=328, Percent_Identity=35.9756097560976, Blast_Score=211, Evalue=6e-56,
Organism=Escherichia coli, GI87081872, Length=325, Percent_Identity=36.9230769230769, Blast_Score=211, Evalue=1e-55,
Organism=Escherichia coli, GI1787583, Length=335, Percent_Identity=34.9253731343284, Blast_Score=200, Evalue=2e-52,
Organism=Escherichia coli, GI1789828, Length=243, Percent_Identity=35.3909465020576, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI87081858, Length=313, Percent_Identity=28.4345047923323, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1786911, Length=124, Percent_Identity=33.8709677419355, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 53025; Mature: 52893

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEQQPRVLLIDDEEDLCTLMQISLSRLGVQTDCAYNLQRAKQLLQQQHYDACLTDLNLP
CCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DGHGLDFVQYVTERFPYLPIAVITAYGNMGIAIAALKAGAFDFVSKPINQQHLSQLIQTA
CCCCCHHHHHHHHHCCCCCEEEEEECCCCCEEEEHHHCCHHHHHHCCCCHHHHHHHHHHH
LHSSQTAPDNEQDTLEHRLLIGQSAPIEQLRIALRKIARSQAPVFITGESGTGKEVVANL
HHCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHH
VHRLSNRNEGPFIALNCGAIPSELMESELFGHKKGSFTGATQDKQGLLLSAHGGSLFLDE
HHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCHHHH
IAELPLTMQVKLLRAVQEKKIRPIGSDREIDVDFRVISASHQNLELLVQQGKFRQDLFFR
HHCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEECCCCCHHHHHHCCCCCCEEEEE
IHVMDIQLPPLRERGQDILLLAKHFIQHVCHEWDLPLKHLSPAAEHYLLHYDFPGNVREL
EEEEEECCCCHHHCCCHHHHHHHHHHHHHHHHCCCCHHHCCCCHHCEEEEECCCCCHHHH
RNMMERAITLSDGASIDLQQLNSSLLRQTTIPELDLTPISNTVSIAPTSQQNLEFPHEGL
HHHHHHHEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCCCCHHHH
EQYLEKVEKDMLLNALNSTHWNRTLAAKKLGMSFRSLRYRLKKFGLDTDDG
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TEQQPRVLLIDDEEDLCTLMQISLSRLGVQTDCAYNLQRAKQLLQQQHYDACLTDLNLP
CCCCCCEEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DGHGLDFVQYVTERFPYLPIAVITAYGNMGIAIAALKAGAFDFVSKPINQQHLSQLIQTA
CCCCCHHHHHHHHHCCCCCEEEEEECCCCCEEEEHHHCCHHHHHHCCCCHHHHHHHHHHH
LHSSQTAPDNEQDTLEHRLLIGQSAPIEQLRIALRKIARSQAPVFITGESGTGKEVVANL
HHCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHH
VHRLSNRNEGPFIALNCGAIPSELMESELFGHKKGSFTGATQDKQGLLLSAHGGSLFLDE
HHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCHHHH
IAELPLTMQVKLLRAVQEKKIRPIGSDREIDVDFRVISASHQNLELLVQQGKFRQDLFFR
HHCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEECCCCCHHHHHHCCCCCCEEEEE
IHVMDIQLPPLRERGQDILLLAKHFIQHVCHEWDLPLKHLSPAAEHYLLHYDFPGNVREL
EEEEEECCCCHHHCCCHHHHHHHHHHHHHHHHCCCCHHHCCCCHHCEEEEECCCCCHHHH
RNMMERAITLSDGASIDLQQLNSSLLRQTTIPELDLTPISNTVSIAPTSQQNLEFPHEGL
HHHHHHHEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCCCCHHHH
EQYLEKVEKDMLLNALNSTHWNRTLAAKKLGMSFRSLRYRLKKFGLDTDDG
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1317379; 8097014; 7911557; 10984043 [H]