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The map label for this gene is nlaIIIM [H]

Identifier: 50083526

GI number: 50083526

Start: 247744

End: 249039

Strand: Direct

Name: nlaIIIM [H]

Synonym: ACIAD0248

Alternate gene names: 50083526

Gene position: 247744-249039 (Clockwise)

Preceding gene: 50083525

Following gene: 50083527

Centisome position: 6.88

GC content: 44.75

Gene sequence:

>1296_bases
ATGAATTCAGAGGCTTCGGTTTATCATAAACGCCGCCATGCGGCACGAACGACAGATGAATATTTGTTTCATCAACTTGT
GCCTTATCTGGGCAATAAGCGTCGTTTGCTGCATCTCATTTTAGAAGCACTGGAAATTACTGGAACGCTCAATAGCAAAA
AAAAGAATCCGCCAATTTTTGCCGATTTTTTTGCTGGTAGCGGAGTTGTTTCACGCTTAGCTAGACAAAATGGTTATCGG
GTCATTGCCAACGACTGGGAACCTTATAGCCATGCATTGAATCATGCGATTTTAGCGTGTGTCGATGATGCGCCAGCGTT
TAAAGAACTTGGCGGCTATCAAAAGGCAATTGATTATCTGAATCGTTTGCCTGAAGTTAAGGGCTGGGTGACACATAACT
TATGTCCACGCAATGACGATATTTATGATCCGAGTCGAGATCGTTTGTTTTTTAAGCGTCGAAATGGAATGCGTATCGAT
GCAATCCGTCAGCAAATCGCGACTTGGCAGGCGCAGGGTGCCATTAATGATGTCGAGATGAGCGCATTGCTGGCGCCATT
ATTATATTCGGCCAGTTTTGTCAGTAATACGAGCGGCGTCTTTAAGAGCTTTCATCAGGGCTGGGGTGGGCGTACCCAAA
CAGCACTTGAACGAATTGAATCATTGCTGTGGTTAACACCCAGCCGGTTTTGTGACGTGGGCGATTCTAAACGTCCTGCC
GCTGAAATGTGGTGTGTAGACGCGCAGCATCTTGCCACTCAGATGAGTAGTTTTGAAGTTGATGTGGCGTATTTGGATCC
ACCTTATAATCAGCATGCCTACAGTAGTAACTATCATGTATTAAATGCTTTAACCTTGTGGGATCAGGTGGATTTACCCT
CACCCGATACCAAAGGCTATAAAAGCGGAATTGACCGTGCTTGGCGTAAAGAACGTCCGAGTCCGTATAATTCGTCTAAG
TATGCAAAAGATGCTTATGAGAAGTTACTTGAAACGATTAATGCACGTTATATTTTGACCAGTTATTCGACAGACGGAAA
TATTGAGCCAAAAGATTTACTCACTGCAAACTTAAAGCGCGGCAAAGTCACGTTATTAACTCAAGATGTGCCACGTTATC
GTGTCAGTAAACAGCGTCAGTCAGAACGTGCACGTGTACTGGAATTTATTGTGATTACAGACACCCATGCCAAATCTGGC
CCGCCATTACGTCAGTTGCTGGGTCAGCTTTATCATTTTGCTGAACTGGGTGGTGTTGATACTTCTGGGAATAGCACCCA
GCTTGCGCTGTGGTAG

Upstream 100 bases:

>100_bases
AGCTAGATAAGATGGCTCAACAATTACGATTTGAACTGATTGACCTGACTCAGCTTGGTCAAGATATCCGTTTAAGGTTA
GTTCCGATTCAAGAGACAGT

Downstream 100 bases:

>100_bases
TTACCGTCTGGTATTGTATTGTGTAGGAATTTGGAGAGATCAACATGTTTACAGGCATTATTGAAAGTTTAGGTCAGGTT
GAAAGCCTCCAGAGTGTTGG

Product: DNA modification methylase

Products: NA

Alternate protein names: M.NlaIII; Adenine-specific methyltransferase NlaIII [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MNSEASVYHKRRHAARTTDEYLFHQLVPYLGNKRRLLHLILEALEITGTLNSKKKNPPIFADFFAGSGVVSRLARQNGYR
VIANDWEPYSHALNHAILACVDDAPAFKELGGYQKAIDYLNRLPEVKGWVTHNLCPRNDDIYDPSRDRLFFKRRNGMRID
AIRQQIATWQAQGAINDVEMSALLAPLLYSASFVSNTSGVFKSFHQGWGGRTQTALERIESLLWLTPSRFCDVGDSKRPA
AEMWCVDAQHLATQMSSFEVDVAYLDPPYNQHAYSSNYHVLNALTLWDQVDLPSPDTKGYKSGIDRAWRKERPSPYNSSK
YAKDAYEKLLETINARYILTSYSTDGNIEPKDLLTANLKRGKVTLLTQDVPRYRVSKQRQSERARVLEFIVITDTHAKSG
PPLRQLLGQLYHFAELGGVDTSGNSTQLALW

Sequences:

>Translated_431_residues
MNSEASVYHKRRHAARTTDEYLFHQLVPYLGNKRRLLHLILEALEITGTLNSKKKNPPIFADFFAGSGVVSRLARQNGYR
VIANDWEPYSHALNHAILACVDDAPAFKELGGYQKAIDYLNRLPEVKGWVTHNLCPRNDDIYDPSRDRLFFKRRNGMRID
AIRQQIATWQAQGAINDVEMSALLAPLLYSASFVSNTSGVFKSFHQGWGGRTQTALERIESLLWLTPSRFCDVGDSKRPA
AEMWCVDAQHLATQMSSFEVDVAYLDPPYNQHAYSSNYHVLNALTLWDQVDLPSPDTKGYKSGIDRAWRKERPSPYNSSK
YAKDAYEKLLETINARYILTSYSTDGNIEPKDLLTANLKRGKVTLLTQDVPRYRVSKQRQSERARVLEFIVITDTHAKSG
PPLRQLLGQLYHFAELGGVDTSGNSTQLALW
>Mature_431_residues
MNSEASVYHKRRHAARTTDEYLFHQLVPYLGNKRRLLHLILEALEITGTLNSKKKNPPIFADFFAGSGVVSRLARQNGYR
VIANDWEPYSHALNHAILACVDDAPAFKELGGYQKAIDYLNRLPEVKGWVTHNLCPRNDDIYDPSRDRLFFKRRNGMRID
AIRQQIATWQAQGAINDVEMSALLAPLLYSASFVSNTSGVFKSFHQGWGGRTQTALERIESLLWLTPSRFCDVGDSKRPA
AEMWCVDAQHLATQMSSFEVDVAYLDPPYNQHAYSSNYHVLNALTLWDQVDLPSPDTKGYKSGIDRAWRKERPSPYNSSK
YAKDAYEKLLETINARYILTSYSTDGNIEPKDLLTANLKRGKVTLLTQDVPRYRVSKQRQSERARVLEFIVITDTHAKSG
PPLRQLLGQLYHFAELGGVDTSGNSTQLALW

Specific function: This methylase recognizes the double-stranded sequence CATG, causes specific methylation on A-2 on both strands and protects the DNA from cleavage by the NlaIII endonuclease [H]

COG id: COG3392

COG function: function code L; Adenine-specific DNA methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 48943; Mature: 48943

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSEASVYHKRRHAARTTDEYLFHQLVPYLGNKRRLLHLILEALEITGTLNSKKKNPPIF
CCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCEE
ADFFAGSGVVSRLARQNGYRVIANDWEPYSHALNHAILACVDDAPAFKELGGYQKAIDYL
EEHHCCCHHHHHHHHHCCCEEEECCCCHHHHHHHHHHEEEECCCHHHHHHCCHHHHHHHH
NRLPEVKGWVTHNLCPRNDDIYDPSRDRLFFKRRNGMRIDAIRQQIATWQAQGAINDVEM
HHCCHHCCHHCCCCCCCCCCCCCCCHHCEEEECCCCCCHHHHHHHHHHHHHCCCCCHHHH
SALLAPLLYSASFVSNTSGVFKSFHQGWGGRTQTALERIESLLWLTPSRFCDVGDSKRPA
HHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHCCCCCCCCCH
AEMWCVDAQHLATQMSSFEVDVAYLDPPYNQHAYSSNYHVLNALTLWDQVDLPSPDTKGY
HHHHHCCHHHHHHHHHHCEEEEEEECCCCCCCCCCCCCEEEEEEHHHHCCCCCCCCCCHH
KSGIDRAWRKERPSPYNSSKYAKDAYEKLLETINARYILTSYSTDGNIEPKDLLTANLKR
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHCCCC
GKVTLLTQDVPRYRVSKQRQSERARVLEFIVITDTHAKSGPPLRQLLGQLYHFAELGGVD
CEEEEEECCCCHHHHHHHHHHHHHHEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCC
TSGNSTQLALW
CCCCCCEEEEC
>Mature Secondary Structure
MNSEASVYHKRRHAARTTDEYLFHQLVPYLGNKRRLLHLILEALEITGTLNSKKKNPPIF
CCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCEE
ADFFAGSGVVSRLARQNGYRVIANDWEPYSHALNHAILACVDDAPAFKELGGYQKAIDYL
EEHHCCCHHHHHHHHHCCCEEEECCCCHHHHHHHHHHEEEECCCHHHHHHCCHHHHHHHH
NRLPEVKGWVTHNLCPRNDDIYDPSRDRLFFKRRNGMRIDAIRQQIATWQAQGAINDVEM
HHCCHHCCHHCCCCCCCCCCCCCCCHHCEEEECCCCCCHHHHHHHHHHHHHCCCCCHHHH
SALLAPLLYSASFVSNTSGVFKSFHQGWGGRTQTALERIESLLWLTPSRFCDVGDSKRPA
HHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHCCCCCCCCCH
AEMWCVDAQHLATQMSSFEVDVAYLDPPYNQHAYSSNYHVLNALTLWDQVDLPSPDTKGY
HHHHHCCHHHHHHHHHHCEEEEEEECCCCCCCCCCCCCEEEEEEHHHHCCCCCCCCCCHH
KSGIDRAWRKERPSPYNSSKYAKDAYEKLLETINARYILTSYSTDGNIEPKDLLTANLKR
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHCCCC
GKVTLLTQDVPRYRVSKQRQSERARVLEFIVITDTHAKSGPPLRQLLGQLYHFAELGGVD
CEEEEEECCCCHHHHHHHHHHHHHHEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCC
TSGNSTQLALW
CCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2277628 [H]