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The map label for this gene is amtB [H]

Identifier: 50083523

GI number: 50083523

Start: 244664

End: 246064

Strand: Direct

Name: amtB [H]

Synonym: ACIAD0245

Alternate gene names: 50083523

Gene position: 244664-246064 (Clockwise)

Preceding gene: 50083522

Following gene: 50083524

Centisome position: 6.8

GC content: 46.75

Gene sequence:

>1401_bases
ATGAAAAAAATGCTTTTAGCGCTAAGTCTATCTGGTGCGCTTTTAGGTGGTTCGGCTGCGTGGGCAGAAAATGCGGCATC
AGCACCGGCAACTGAAGAAACAACAACCGTTGTAGCTACAACGACTGAAGCACCTGTAACTGGCTCGGCAGAAGCACCTT
CCGCCGCTACTGCTACACCAGCGCCAGCAGCAACACCCAAAATGGATTCGGGTGATACAGCATGGATTCTGATTTCTACT
GCTTTAGTTCTTTTAATGACCATTCCTGGTTTAGCACTTTTTTATGGTGGTATGGTACGTAAGAAAAACGTATTAAGCAC
TATGGCACATAGCTTTGTCGCTGCTGCAATTGTCAGTATTGTTTGGGTGGTGATTGGTTATACCTTGGCATTTAGTACTA
CAGGCAATGCCTTTGTTGGTGGTCTGGATAAAATCATGCTGTCGGGTATTACCACAGAGGCCTTGAGTGGTACCATTCCT
GAGATTCTTTTTGTTATTTTCCAAATGACCTTTGCGATTATTACTGTTGCCATTATTAGTGGCTCGGTTGCTGAACGTAT
GAAGTTTGGAGCCTTTGTTGCATTTATCACCATTTGGGTCATCGTGGTGTATGCGCCAATTACCCACTGGGTATGGGGTG
GTGGCTGGTTGGGTAGCGATGGCGCACTTGACTTTGCTGGTGGTACCGTTGTTCATATCAATTCGGGTGTTGCTGGTCTG
GTTGCTGCGTATATGCTCGGTAAGCGTATTGGGCTGGGTAAAGAATCAATGGCACCACACAACCTTGCACTCACAGTGAT
TGGCGCAAGCTTGCTCTGGGTAGGTTGGTTCGGTTTCAATGGTGGTTCTGCACTTGCTGCAAATGGTGCTGCAGGCTATG
CCTTGGTGACGACTCAGGTTGCAGCAGCAGCAGCGGCAATTTCTTGGGGTATTGTTGAGAAAATTGTTCGCGGTAAAACT
TCATTGCTTGGTCTTCTTTCAGGTGCAGTTGCAGGTCTGGTTGTGATTACACCGGCTGCAGGCTTTGTTACTGTAAGTGG
CGCGTTGATCATGGGATTGATTGGTGGTGTCGTTTGCTTCTGGGGAATTTCTGGCCTAAAACGTATGTTGAAAGCAGACG
ACTCATTGGATGCATTTGGTCTGCATGCCGTAGGTGGTATGGCAGGTGCAATCTTGACGGCTGTATTTGCAAGTCCATTC
ATCATGGGTGACAAAGCGCCAGCAAGTGTGACACATCAGCTCTGGATTCAAATAGAAGGTGTATTGGCTACAATTGCATA
TAGTGCTGTGTTGACCTTTATTATCTTGAAAGTCATTGATGTCGTGATTGGAATTCGTGCTAACTCTGATGATGAGCGTA
TGGGTCTCGATTTGAGCCAGCATGGCGAACGTGTAGAATAA

Upstream 100 bases:

>100_bases
CGTATTCGTACTGGTGAAACTGGTGCAGATGCTGTTTAAGTGTTGGCATAAAGTTTGCTGAGTACAAATAACTCATAAAA
ATTGAGGTTGGGGGATACGA

Downstream 100 bases:

>100_bases
TTATTGAGTTCTTTAAAAAACAGCCGATCAGGCTGTTTTTTTATGGCGTTTTGTGGTCGAGATTCTGTAAGCGTTTAAAT
TTTGCTACACTAGCTTAAAT

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MKKMLLALSLSGALLGGSAAWAENAASAPATEETTTVVATTTEAPVTGSAEAPSAATATPAPAATPKMDSGDTAWILIST
ALVLLMTIPGLALFYGGMVRKKNVLSTMAHSFVAAAIVSIVWVVIGYTLAFSTTGNAFVGGLDKIMLSGITTEALSGTIP
EILFVIFQMTFAIITVAIISGSVAERMKFGAFVAFITIWVIVVYAPITHWVWGGGWLGSDGALDFAGGTVVHINSGVAGL
VAAYMLGKRIGLGKESMAPHNLALTVIGASLLWVGWFGFNGGSALAANGAAGYALVTTQVAAAAAAISWGIVEKIVRGKT
SLLGLLSGAVAGLVVITPAAGFVTVSGALIMGLIGGVVCFWGISGLKRMLKADDSLDAFGLHAVGGMAGAILTAVFASPF
IMGDKAPASVTHQLWIQIEGVLATIAYSAVLTFIILKVIDVVIGIRANSDDERMGLDLSQHGERVE

Sequences:

>Translated_466_residues
MKKMLLALSLSGALLGGSAAWAENAASAPATEETTTVVATTTEAPVTGSAEAPSAATATPAPAATPKMDSGDTAWILIST
ALVLLMTIPGLALFYGGMVRKKNVLSTMAHSFVAAAIVSIVWVVIGYTLAFSTTGNAFVGGLDKIMLSGITTEALSGTIP
EILFVIFQMTFAIITVAIISGSVAERMKFGAFVAFITIWVIVVYAPITHWVWGGGWLGSDGALDFAGGTVVHINSGVAGL
VAAYMLGKRIGLGKESMAPHNLALTVIGASLLWVGWFGFNGGSALAANGAAGYALVTTQVAAAAAAISWGIVEKIVRGKT
SLLGLLSGAVAGLVVITPAAGFVTVSGALIMGLIGGVVCFWGISGLKRMLKADDSLDAFGLHAVGGMAGAILTAVFASPF
IMGDKAPASVTHQLWIQIEGVLATIAYSAVLTFIILKVIDVVIGIRANSDDERMGLDLSQHGERVE
>Mature_466_residues
MKKMLLALSLSGALLGGSAAWAENAASAPATEETTTVVATTTEAPVTGSAEAPSAATATPAPAATPKMDSGDTAWILIST
ALVLLMTIPGLALFYGGMVRKKNVLSTMAHSFVAAAIVSIVWVVIGYTLAFSTTGNAFVGGLDKIMLSGITTEALSGTIP
EILFVIFQMTFAIITVAIISGSVAERMKFGAFVAFITIWVIVVYAPITHWVWGGGWLGSDGALDFAGGTVVHINSGVAGL
VAAYMLGKRIGLGKESMAPHNLALTVIGASLLWVGWFGFNGGSALAANGAAGYALVTTQVAAAAAAISWGIVEKIVRGKT
SLLGLLSGAVAGLVVITPAAGFVTVSGALIMGLIGGVVCFWGISGLKRMLKADDSLDAFGLHAVGGMAGAILTAVFASPF
IMGDKAPASVTHQLWIQIEGVLATIAYSAVLTFIILKVIDVVIGIRANSDDERMGLDLSQHGERVE

Specific function: Involved in the uptake of ammonia [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=414, Percent_Identity=49.7584541062802, Blast_Score=392, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17550182, Length=434, Percent_Identity=27.8801843317972, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17550180, Length=427, Percent_Identity=28.8056206088993, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI32564042, Length=434, Percent_Identity=26.7281105990783, Blast_Score=113, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI133931100, Length=432, Percent_Identity=26.3888888888889, Blast_Score=112, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6325396, Length=417, Percent_Identity=36.9304556354916, Blast_Score=258, Evalue=1e-69,
Organism=Saccharomyces cerevisiae, GI6324187, Length=418, Percent_Identity=36.6028708133971, Blast_Score=254, Evalue=2e-68,
Organism=Saccharomyces cerevisiae, GI6321559, Length=421, Percent_Identity=34.2042755344418, Blast_Score=241, Evalue=1e-64,
Organism=Drosophila melanogaster, GI161078314, Length=411, Percent_Identity=29.1970802919708, Blast_Score=133, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 47584; Mature: 47584

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKMLLALSLSGALLGGSAAWAENAASAPATEETTTVVATTTEAPVTGSAEAPSAATATP
CCCEEEEEECCCHHCCCCHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCC
APAATPKMDSGDTAWILISTALVLLMTIPGLALFYGGMVRKKNVLSTMAHSFVAAAIVSI
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
VWVVIGYTLAFSTTGNAFVGGLDKIMLSGITTEALSGTIPEILFVIFQMTFAIITVAIIS
HHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHC
GSVAERMKFGAFVAFITIWVIVVYAPITHWVWGGGWLGSDGALDFAGGTVVHINSGVAGL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCEEECCCEEEEECCCHHHH
VAAYMLGKRIGLGKESMAPHNLALTVIGASLLWVGWFGFNGGSALAANGAAGYALVTTQV
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHEEECCCCCCEEECCCCCCHHHHHHHH
AAAAAAISWGIVEKIVRGKTSLLGLLSGAVAGLVVITPAAGFVTVSGALIMGLIGGVVCF
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCEEEEECCCCHHHHHHHHHHHHHHHHHHH
WGISGLKRMLKADDSLDAFGLHAVGGMAGAILTAVFASPFIMGDKAPASVTHQLWIQIEG
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHH
VLATIAYSAVLTFIILKVIDVVIGIRANSDDERMGLDLSQHGERVE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCHHHCCCCCC
>Mature Secondary Structure
MKKMLLALSLSGALLGGSAAWAENAASAPATEETTTVVATTTEAPVTGSAEAPSAATATP
CCCEEEEEECCCHHCCCCHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCC
APAATPKMDSGDTAWILISTALVLLMTIPGLALFYGGMVRKKNVLSTMAHSFVAAAIVSI
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
VWVVIGYTLAFSTTGNAFVGGLDKIMLSGITTEALSGTIPEILFVIFQMTFAIITVAIIS
HHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHC
GSVAERMKFGAFVAFITIWVIVVYAPITHWVWGGGWLGSDGALDFAGGTVVHINSGVAGL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCEEECCCEEEEECCCHHHH
VAAYMLGKRIGLGKESMAPHNLALTVIGASLLWVGWFGFNGGSALAANGAAGYALVTTQV
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHEEECCCCCCEEECCCCCCHHHHHHHH
AAAAAAISWGIVEKIVRGKTSLLGLLSGAVAGLVVITPAAGFVTVSGALIMGLIGGVVCF
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCEEEEECCCCHHHHHHHHHHHHHHHHHHH
WGISGLKRMLKADDSLDAFGLHAVGGMAGAILTAVFASPFIMGDKAPASVTHQLWIQIEG
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHH
VLATIAYSAVLTFIILKVIDVVIGIRANSDDERMGLDLSQHGERVE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]