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The map label for this gene is nepI [H]

Identifier: 50083477

GI number: 50083477

Start: 189942

End: 191147

Strand: Reverse

Name: nepI [H]

Synonym: ACIAD0195

Alternate gene names: 50083477

Gene position: 191147-189942 (Counterclockwise)

Preceding gene: 50083490

Following gene: 50083476

Centisome position: 5.31

GC content: 44.03

Gene sequence:

>1206_bases
ATGAATCTTTCTGAGGCTCAGCCCGTTCGCCCCAGTCAAGGCAGCTGGTTTGCCATTCTTGCCGTTTCAATTGCTGCATT
TGCACTGGTCACCAGTGAATTCTTACCTATTGGTGTACTGAATGATGTCGCCAATGATCTGAAAATCTCTGTAGGAACAG
CAGGCCTGATTATTACCTTGCCAGGTGTAATGGCCGCACTTGCCGCTCCTCTGCTTCCTGTCGCCTTTAAACAACTTGAT
CGCCGCTATGTCTTATTGTTTTTAACCGCCGTGATGGTCATTGCCAATGCGATTACTGCTTTTGCCAGTGATTTTACTCT
TCTACTGATTAGTCGCATGATCTTGGGTATTTCAATTGGTGGCTTCTGGGCAACGGCGATTGCTTTAAGTGGCAAGCTTG
CACCTGTACATTTACCTATCGCCAAAGCCACAGCAGTTGTGATGGCTGGTGTCACTTTCGCAACGGTACTTGGTGTACCA
ATAGGTACATGGTTAAGTGAGTTCTATGGATGGCGCAGTGCCTTTGGTATTACGGCTATCATTGGGTTTGTGGTGTTTCT
GCTACAGCTTCGCTATTTGCCAAAACTGATTCCAGAATCCTCAATTCGATTACACGATTTACCTGCGCTGTTACGTGTAC
CCAAAGCACGAAGTGGCATGCTGATTATTTTATTGATCGGGCTGGCACATTTTTGTGCGTATAGTTATCTGGCACCTTTT
TTCAAAAATGTGGCCGGATTTAATGGTACGACCATTAGCAGTCTGTTATTGCTGTATGGTATTGCAGGTATCTTTGGAAA
CGCATTCGCAGGTTATAGTGGTAATTTCAATATTCGTTATACACTGGCATTTGTCGGCTTGTGTTTTGCAGTGGTCTTCT
TTAGCTTTCCAGTCTTTGCTGTTCACGAGGCAGGTGCTATTGTACTCACCGCTTTATGGGGGTTTGCCTTCGGAGCCTTC
CCAACATCGGCCAATATCTGGATGTTTGTACATGCACCTCAGGCTGTTGAAAAAGGAATGCCATTATTTGTAGGACTGTT
TCAAGTCATGATTGCGACAGGCTCATTGCTCGGGGGCTATATCGTCGATCATTTCAATGAAAACATTTTAATTTACGGTG
TGCTGAATTTTATTATACTGGCGTTGATTAGTGTTTTTACGCTGGCAAAAGGTTTAAATAATCCTAAGCTTACTTGTGAA
TCATAA

Upstream 100 bases:

>100_bases
ATGTTCAACTATTTTTATTTTCATAGTGATCATTATTAAATTAAAACACGCTCTCATCTATTCGTCATCTCTCTTTATCA
GGATTAGAATCATGGAAACA

Downstream 100 bases:

>100_bases
TTTATCCTCGTATCCAATATGACCATGATCTCTCCCATAAAGATGAATATGCCAACTGATGATCTGACTCGGTTAATTGA
GCTTCATGAGTTCTTTTTCC

Product: MFS family transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MNLSEAQPVRPSQGSWFAILAVSIAAFALVTSEFLPIGVLNDVANDLKISVGTAGLIITLPGVMAALAAPLLPVAFKQLD
RRYVLLFLTAVMVIANAITAFASDFTLLLISRMILGISIGGFWATAIALSGKLAPVHLPIAKATAVVMAGVTFATVLGVP
IGTWLSEFYGWRSAFGITAIIGFVVFLLQLRYLPKLIPESSIRLHDLPALLRVPKARSGMLIILLIGLAHFCAYSYLAPF
FKNVAGFNGTTISSLLLLYGIAGIFGNAFAGYSGNFNIRYTLAFVGLCFAVVFFSFPVFAVHEAGAIVLTALWGFAFGAF
PTSANIWMFVHAPQAVEKGMPLFVGLFQVMIATGSLLGGYIVDHFNENILIYGVLNFIILALISVFTLAKGLNNPKLTCE
S

Sequences:

>Translated_401_residues
MNLSEAQPVRPSQGSWFAILAVSIAAFALVTSEFLPIGVLNDVANDLKISVGTAGLIITLPGVMAALAAPLLPVAFKQLD
RRYVLLFLTAVMVIANAITAFASDFTLLLISRMILGISIGGFWATAIALSGKLAPVHLPIAKATAVVMAGVTFATVLGVP
IGTWLSEFYGWRSAFGITAIIGFVVFLLQLRYLPKLIPESSIRLHDLPALLRVPKARSGMLIILLIGLAHFCAYSYLAPF
FKNVAGFNGTTISSLLLLYGIAGIFGNAFAGYSGNFNIRYTLAFVGLCFAVVFFSFPVFAVHEAGAIVLTALWGFAFGAF
PTSANIWMFVHAPQAVEKGMPLFVGLFQVMIATGSLLGGYIVDHFNENILIYGVLNFIILALISVFTLAKGLNNPKLTCE
S
>Mature_401_residues
MNLSEAQPVRPSQGSWFAILAVSIAAFALVTSEFLPIGVLNDVANDLKISVGTAGLIITLPGVMAALAAPLLPVAFKQLD
RRYVLLFLTAVMVIANAITAFASDFTLLLISRMILGISIGGFWATAIALSGKLAPVHLPIAKATAVVMAGVTFATVLGVP
IGTWLSEFYGWRSAFGITAIIGFVVFLLQLRYLPKLIPESSIRLHDLPALLRVPKARSGMLIILLIGLAHFCAYSYLAPF
FKNVAGFNGTTISSLLLLYGIAGIFGNAFAGYSGNFNIRYTLAFVGLCFAVVFFSFPVFAVHEAGAIVLTALWGFAFGAF
PTSANIWMFVHAPQAVEKGMPLFVGLFQVMIATGSLLGGYIVDHFNENILIYGVLNFIILALISVFTLAKGLNNPKLTCE
S

Specific function: Involved in the efflux of purine ribonucleosides, such as guanosine, adenosine and especially inosine. Involved in the resistance to 6-mercaptopurine [H]

COG id: COG2814

COG function: function code G; Arabinose efflux permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. NepI (TC 2.A.1.2.26) subfamily [H]

Homologues:

Organism=Escherichia coli, GI145693198, Length=355, Percent_Identity=29.5774647887324, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI1787808, Length=364, Percent_Identity=30.4945054945055, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI1787947, Length=300, Percent_Identity=29.6666666666667, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1786595, Length=264, Percent_Identity=28.7878787878788, Blast_Score=110, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42824; Mature: 42824

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLSEAQPVRPSQGSWFAILAVSIAAFALVTSEFLPIGVLNDVANDLKISVGTAGLIITL
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCEEEECCCCCEEEH
PGVMAALAAPLLPVAFKQLDRRYVLLFLTAVMVIANAITAFASDFTLLLISRMILGISIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
GFWATAIALSGKLAPVHLPIAKATAVVMAGVTFATVLGVPIGTWLSEFYGWRSAFGITAI
HHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
IGFVVFLLQLRYLPKLIPESSIRLHDLPALLRVPKARSGMLIILLIGLAHFCAYSYLAPF
HHHHHHHHHHHHHHHHCCCCCCCHHCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
FKNVAGFNGTTISSLLLLYGIAGIFGNAFAGYSGNFNIRYTLAFVGLCFAVVFFSFPVFA
HHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCHHH
VHEAGAIVLTALWGFAFGAFPTSANIWMFVHAPQAVEKGMPLFVGLFQVMIATGSLLGGY
HHCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHCCCHHHHHHHHHHHHHHHHHHHH
IVDHFNENILIYGVLNFIILALISVFTLAKGLNNPKLTCES
HHHHCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCCEECC
>Mature Secondary Structure
MNLSEAQPVRPSQGSWFAILAVSIAAFALVTSEFLPIGVLNDVANDLKISVGTAGLIITL
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCEEEECCCCCEEEH
PGVMAALAAPLLPVAFKQLDRRYVLLFLTAVMVIANAITAFASDFTLLLISRMILGISIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
GFWATAIALSGKLAPVHLPIAKATAVVMAGVTFATVLGVPIGTWLSEFYGWRSAFGITAI
HHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
IGFVVFLLQLRYLPKLIPESSIRLHDLPALLRVPKARSGMLIILLIGLAHFCAYSYLAPF
HHHHHHHHHHHHHHHHCCCCCCCHHCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
FKNVAGFNGTTISSLLLLYGIAGIFGNAFAGYSGNFNIRYTLAFVGLCFAVVFFSFPVFA
HHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCHHH
VHEAGAIVLTALWGFAFGAFPTSANIWMFVHAPQAVEKGMPLFVGLFQVMIATGSLLGGY
HHCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHCCCHHHHHHHHHHHHHHHHHHHH
IVDHFNENILIYGVLNFIILALISVFTLAKGLNNPKLTCES
HHHHCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA