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The map label for this gene is rhtB [H]

Identifier: 50083457

GI number: 50083457

Start: 174698

End: 175324

Strand: Direct

Name: rhtB [H]

Synonym: ACIAD0173

Alternate gene names: 50083457

Gene position: 174698-175324 (Clockwise)

Preceding gene: 50083455

Following gene: 50083461

Centisome position: 4.85

GC content: 43.86

Gene sequence:

>627_bases
ATGTCGTTTCAGATTTGGATAGGATTTATGTTGGCGTGCTGGGTGATTAGTCTATCTCCTGGCGCAGGCGCTATTGCCTC
AATGTCGAGTGGTTTAAATTATGGCTTTTGCCGTGGTTATTGGAATGCACTCGGTTTGCAATTGGCACTTTTGGTTCAGA
TTGGTATTGTTGCCGCAGGCGTAGGAATGCTATTTACCACTACACCATGGGCATTTTTGCTGGTGAAATGGTTTGGTATT
GGTTATTTGTTGTATTTGGCTTATTTACAGTGGACGGCTCCTGCTCAGCCCATTCAGATTCAATCTGAGCAACATGAAAT
CTCACGTCAACGCCTTATAATTAAAGGCTTTATTGTGAATATTAGTAATCCGAAAGCGATGGTGTTCTTGTTGGCGGTGT
TGCCACAATTTATTGACTTAACCCGCCCACAAGCACCGCAATACGCGATTATGGCGGTTACCATGATATTGATTGATCTG
ATAGTTATGGCAGGTTATACAGGGCTGGCTGCCAAGGTAATGCGACTGCTGCGCTCACCGCGACAACAAAAATATCTGAA
TCGGAGTTTTGCTGTTATGTTTAGTGGCGCTGCCTTCCTATTAAGTCTTGTACATCAGGCTCAATAA

Upstream 100 bases:

>100_bases
ATACCCAGCAAGGATATTTTTATCGATGACAACAATCGGATCAATTAGAATCGAATCAATGTAACAGGGTATAATCAATT
TTTTCCTTTATTTTTGGGTT

Downstream 100 bases:

>100_bases
TTCTAAATAGAATGAGTCTGACGTTTGGCTTTGATTTTCTGAAATAAAAAAATTCCGCCAAACAGTATCGACATGCAAAG
CACAATACTCAGACCTGTGG

Product: homoserine/homoserine lactone efflux protein

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 208; Mature: 207

Protein sequence:

>208_residues
MSFQIWIGFMLACWVISLSPGAGAIASMSSGLNYGFCRGYWNALGLQLALLVQIGIVAAGVGMLFTTTPWAFLLVKWFGI
GYLLYLAYLQWTAPAQPIQIQSEQHEISRQRLIIKGFIVNISNPKAMVFLLAVLPQFIDLTRPQAPQYAIMAVTMILIDL
IVMAGYTGLAAKVMRLLRSPRQQKYLNRSFAVMFSGAAFLLSLVHQAQ

Sequences:

>Translated_208_residues
MSFQIWIGFMLACWVISLSPGAGAIASMSSGLNYGFCRGYWNALGLQLALLVQIGIVAAGVGMLFTTTPWAFLLVKWFGI
GYLLYLAYLQWTAPAQPIQIQSEQHEISRQRLIIKGFIVNISNPKAMVFLLAVLPQFIDLTRPQAPQYAIMAVTMILIDL
IVMAGYTGLAAKVMRLLRSPRQQKYLNRSFAVMFSGAAFLLSLVHQAQ
>Mature_207_residues
SFQIWIGFMLACWVISLSPGAGAIASMSSGLNYGFCRGYWNALGLQLALLVQIGIVAAGVGMLFTTTPWAFLLVKWFGIG
YLLYLAYLQWTAPAQPIQIQSEQHEISRQRLIIKGFIVNISNPKAMVFLLAVLPQFIDLTRPQAPQYAIMAVTMILIDLI
VMAGYTGLAAKVMRLLRSPRQQKYLNRSFAVMFSGAAFLLSLVHQAQ

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=194, Percent_Identity=39.6907216494845, Blast_Score=147, Evalue=6e-37,
Organism=Escherichia coli, GI1788099, Length=192, Percent_Identity=26.5625, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 23052; Mature: 22921

Theoretical pI: Translated: 10.28; Mature: 10.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFQIWIGFMLACWVISLSPGAGAIASMSSGLNYGFCRGYWNALGLQLALLVQIGIVAAG
CCCHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VGMLFTTTPWAFLLVKWFGIGYLLYLAYLQWTAPAQPIQIQSEQHEISRQRLIIKGFIVN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHHHHHHEEEE
ISNPKAMVFLLAVLPQFIDLTRPQAPQYAIMAVTMILIDLIVMAGYTGLAAKVMRLLRSP
CCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCC
RQQKYLNRSFAVMFSGAAFLLSLVHQAQ
HHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SFQIWIGFMLACWVISLSPGAGAIASMSSGLNYGFCRGYWNALGLQLALLVQIGIVAAG
CCHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VGMLFTTTPWAFLLVKWFGIGYLLYLAYLQWTAPAQPIQIQSEQHEISRQRLIIKGFIVN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHHHHHHEEEE
ISNPKAMVFLLAVLPQFIDLTRPQAPQYAIMAVTMILIDLIVMAGYTGLAAKVMRLLRSP
CCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCC
RQQKYLNRSFAVMFSGAAFLLSLVHQAQ
HHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]