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The map label for this gene is yiaE [C]

Identifier: 50083421

GI number: 50083421

Start: 137774

End: 138742

Strand: Reverse

Name: yiaE [C]

Synonym: ACIAD0133

Alternate gene names: 50083421

Gene position: 138742-137774 (Counterclockwise)

Preceding gene: 50083434

Following gene: 50083416

Centisome position: 3.86

GC content: 37.77

Gene sequence:

>969_bases
TTGAGTGCAAACATGACACCACTAAAAATTACATGTACTGAAAAATATGTACTCATCGATCAAGATTTCAAATTTGATTT
TCCTGTTGAATACACAGAGTATCAACAGCTCTCTCAAGACCAGCTTTATCATCAAGTATTCGATCAAGACGTTATTATTA
TTAGCGATTTGATCATTGATGAACAGGTTCTTAAAAACAATCCAAATCTAAAACTACTGGCACTGTGTTCAACAGGCTAT
AACCACGTCAATATCGAGTTATTGCGCCAGCATAATGTTCAGGTCTGCAACATTCGCGGTTATGCTGGTGATGCTGTTGC
AGAGCATGCTTTTACGCTGATGATCCAGCTGATTAAAAATTTTTCTCAACAGGTTGAAGGCGTTAAACAAGGACTATGGG
GAACAGGACAAAGTTCTTTTTATCTAGCAGCCCCTATGCGTGAACTTAAAGGAAAAACATTAACGATCTTGGGTAAAGGT
GAAATTGGTGAATCACTTGCACAAAAAGCCCGAGCTTTTGGCATGCATATAATTTTTAGCGAACGAAAAAATGCATCACA
GTGTCGTGAAGGTTATGTTCCTTTTGAACAGGCCATTCAACAAGCTGATATTCTTTCACTGCATTGTGAACTCAACCAAT
CTACTCACCATCTCATTGATCATTCAGTTTTATCTCAAATGAAACCCGAAAGTATTCTAATTAATGTAGGACGAGGGGGT
TTAGTCAAGGATTCCGATCTCATTGAAGCATTACTTAATCATCAATTATCTGGATTTGGTGCAGACGTTTTGGATCAGGA
ACCACCAGCAAAAGATCACCCACTTCTCATGTTGCAGCATCAGCACCCAAATGTTCTGATTACTGGGCATATTGCTTGGG
GAACAGAAGAGGCTCAACAGCGTTTATTTGATATTTTGCAAGATAATATTAATAAAAACATGAACGGTATTGCACAAAAT
TTAGTTTAA

Upstream 100 bases:

>100_bases
CTAACTGCACCGAATCGTTTTTCCATTTGAAACATACAGCACGCTAGTAACTATTATAGTTTTGCCAAGGACTGTTGTAT
GATATCTTATCTTTAGTTCA

Downstream 100 bases:

>100_bases
TTCTGGCATGGCGTTAGTGGTTATTCTCACCAACGTCATGTAATAAAAGTATTATTTTCAATTTTTTTATTGCTTGAATT
TTTTGATACTTTTTGAAAGG

Product: 2-hydroxyacid dehydrogenase

Products: hydroxypyruvate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MSANMTPLKITCTEKYVLIDQDFKFDFPVEYTEYQQLSQDQLYHQVFDQDVIIISDLIIDEQVLKNNPNLKLLALCSTGY
NHVNIELLRQHNVQVCNIRGYAGDAVAEHAFTLMIQLIKNFSQQVEGVKQGLWGTGQSSFYLAAPMRELKGKTLTILGKG
EIGESLAQKARAFGMHIIFSERKNASQCREGYVPFEQAIQQADILSLHCELNQSTHHLIDHSVLSQMKPESILINVGRGG
LVKDSDLIEALLNHQLSGFGADVLDQEPPAKDHPLLMLQHQHPNVLITGHIAWGTEEAQQRLFDILQDNINKNMNGIAQN
LV

Sequences:

>Translated_322_residues
MSANMTPLKITCTEKYVLIDQDFKFDFPVEYTEYQQLSQDQLYHQVFDQDVIIISDLIIDEQVLKNNPNLKLLALCSTGY
NHVNIELLRQHNVQVCNIRGYAGDAVAEHAFTLMIQLIKNFSQQVEGVKQGLWGTGQSSFYLAAPMRELKGKTLTILGKG
EIGESLAQKARAFGMHIIFSERKNASQCREGYVPFEQAIQQADILSLHCELNQSTHHLIDHSVLSQMKPESILINVGRGG
LVKDSDLIEALLNHQLSGFGADVLDQEPPAKDHPLLMLQHQHPNVLITGHIAWGTEEAQQRLFDILQDNINKNMNGIAQN
LV
>Mature_321_residues
SANMTPLKITCTEKYVLIDQDFKFDFPVEYTEYQQLSQDQLYHQVFDQDVIIISDLIIDEQVLKNNPNLKLLALCSTGYN
HVNIELLRQHNVQVCNIRGYAGDAVAEHAFTLMIQLIKNFSQQVEGVKQGLWGTGQSSFYLAAPMRELKGKTLTILGKGE
IGESLAQKARAFGMHIIFSERKNASQCREGYVPFEQAIQQADILSLHCELNQSTHHLIDHSVLSQMKPESILINVGRGGL
VKDSDLIEALLNHQLSGFGADVLDQEPPAKDHPLLMLQHQHPNVLITGHIAWGTEEAQQRLFDILQDNINKNMNGIAQNL
V

Specific function: Unknown

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI145580575, Length=293, Percent_Identity=25.2559726962457, Blast_Score=114, Evalue=8e-26,
Organism=Homo sapiens, GI145580578, Length=294, Percent_Identity=25.1700680272109, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI4557499, Length=294, Percent_Identity=25.1700680272109, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI6912396, Length=264, Percent_Identity=25.7575757575758, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI23308577, Length=281, Percent_Identity=27.4021352313167, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI4557497, Length=293, Percent_Identity=23.2081911262799, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI61743967, Length=293, Percent_Identity=23.2081911262799, Blast_Score=104, Evalue=1e-22,
Organism=Escherichia coli, GI87082289, Length=268, Percent_Identity=28.7313432835821, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI1787645, Length=243, Percent_Identity=28.8065843621399, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI1789279, Length=273, Percent_Identity=28.5714285714286, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI1788660, Length=230, Percent_Identity=25.2173913043478, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17532191, Length=288, Percent_Identity=28.8194444444444, Blast_Score=117, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI25147481, Length=293, Percent_Identity=25.938566552901, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6324964, Length=287, Percent_Identity=24.390243902439, Blast_Score=107, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6324055, Length=247, Percent_Identity=27.1255060728745, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6322116, Length=309, Percent_Identity=25.2427184466019, Blast_Score=93, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6320925, Length=292, Percent_Identity=25.6849315068493, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6324980, Length=177, Percent_Identity=25.9887005649718, Blast_Score=77, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6321253, Length=238, Percent_Identity=24.7899159663866, Blast_Score=63, Evalue=7e-11,
Organism=Drosophila melanogaster, GI24646446, Length=287, Percent_Identity=26.4808362369338, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24646448, Length=287, Percent_Identity=26.4808362369338, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24646452, Length=287, Percent_Identity=26.4808362369338, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24646450, Length=287, Percent_Identity=26.4808362369338, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI62472511, Length=287, Percent_Identity=26.4808362369338, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI28571528, Length=301, Percent_Identity=28.2392026578073, Blast_Score=111, Evalue=5e-25,
Organism=Drosophila melanogaster, GI28574286, Length=255, Percent_Identity=26.6666666666667, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI19921140, Length=290, Percent_Identity=26.8965517241379, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24585516, Length=251, Percent_Identity=25.8964143426295, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24585514, Length=255, Percent_Identity=23.1372549019608, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI28574282, Length=255, Percent_Identity=23.1372549019608, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI45552429, Length=255, Percent_Identity=23.1372549019608, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI28574284, Length=255, Percent_Identity=23.1372549019608, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI45551003, Length=255, Percent_Identity=23.1372549019608, Blast_Score=88, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: 1.1.1.29

Molecular weight: Translated: 36209; Mature: 36078

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSANMTPLKITCTEKYVLIDQDFKFDFPVEYTEYQQLSQDQLYHQVFDQDVIIISDLIID
CCCCCCEEEEEECCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEHHHHHH
EQVLKNNPNLKLLALCSTGYNHVNIELLRQHNVQVCNIRGYAGDAVAEHAFTLMIQLIKN
HHHHCCCCCEEEEEEECCCCCEEEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHH
FSQQVEGVKQGLWGTGQSSFYLAAPMRELKGKTLTILGKGEIGESLAQKARAFGMHIIFS
HHHHHHHHHHCCCCCCCCCEEEECCHHHHCCCEEEEEECCCHHHHHHHHHHHHCEEEEEE
ERKNASQCREGYVPFEQAIQQADILSLHCELNQSTHHLIDHSVLSQMKPESILINVGRGG
CCCCHHHHHHCCCCHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCC
LVKDSDLIEALLNHQLSGFGADVLDQEPPAKDHPLLMLQHQHPNVLITGHIAWGTEEAQQ
CCCCHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCEEEEEECCCCEEEEEEEECCHHHHHH
RLFDILQDNINKNMNGIAQNLV
HHHHHHHHHHCCCHHHHHHHCC
>Mature Secondary Structure 
SANMTPLKITCTEKYVLIDQDFKFDFPVEYTEYQQLSQDQLYHQVFDQDVIIISDLIID
CCCCCEEEEEECCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEHHHHHH
EQVLKNNPNLKLLALCSTGYNHVNIELLRQHNVQVCNIRGYAGDAVAEHAFTLMIQLIKN
HHHHCCCCCEEEEEEECCCCCEEEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHH
FSQQVEGVKQGLWGTGQSSFYLAAPMRELKGKTLTILGKGEIGESLAQKARAFGMHIIFS
HHHHHHHHHHCCCCCCCCCEEEECCHHHHCCCEEEEEECCCHHHHHHHHHHHHCEEEEEE
ERKNASQCREGYVPFEQAIQQADILSLHCELNQSTHHLIDHSVLSQMKPESILINVGRGG
CCCCHHHHHHCCCCHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCC
LVKDSDLIEALLNHQLSGFGADVLDQEPPAKDHPLLMLQHQHPNVLITGHIAWGTEEAQQ
CCCCHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCEEEEEECCCCEEEEEEEECCHHHHHH
RLFDILQDNINKNMNGIAQNLV
HHHHHHHHHHCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (R)-glycerate; NAD+

Specific reaction: (R)-glycerate + NAD+ = hydroxypyruvate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]