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The map label for this gene is gudD [H]

Identifier: 50083417

GI number: 50083417

Start: 132209

End: 133543

Strand: Direct

Name: gudD [H]

Synonym: ACIAD0128

Alternate gene names: 50083417

Gene position: 132209-133543 (Clockwise)

Preceding gene: 50083413

Following gene: 50083418

Centisome position: 3.67

GC content: 42.85

Gene sequence:

>1335_bases
ATGGCAGCCTCAACACCAATCATTCAGTCCGTTCGAGCCATACCTGTTGCAGGACACGATAGTATGCTGCTCAATCTAAG
TGGTGCACATGCCCCATATTTCACGCGTAATCTACTAGTCATAGAAGATAATTCTGGAAACATTGGTGTGGGTGAAATCC
CTGGCGGAGAAAAAATTCTAGCCACGCTTAATGATGCCAAGTCACTGATCTTGGGTCAGCCTATTGGGGAATATAAGAAT
TTACTTAAAAAAATCCATCAGACATTTGCAGATCGGGATAGTGGTGGGCGAGGCAATCAAACCTTTGATTTGAGAACCAC
TGTTCATGTCGTGACTGCTTATGAATCCGCATTACTGGATTTGCTTGGAAAACATTTGAACGTCAATGTTGCGAGCTTAC
TCGGTGATGGTCAGCAACGCGATGAAGTTGAAGTACTAGGCTATTTATTCTTTATTGGAGATCGTAAACAGACCTCTCTA
GATTATGCAACTTCTACGCATTTAAATCATGACTGGTATCAAGTGCGTCATGAAAAAGCACTGACACCAGAGGCAATACA
ACGTTTAGCTGAAGCCTCTTATGATCGTTATGGCTTTAAAGATTTTAAATTAAAAGGTGGCGTATTACACGGTGAGCAGG
AGGCTGAAGCAGTCACTGCAATTGCACGACGTTTTCCAGATGCACGTGTCACACTTGATCCAAATGGCGCTTGGTATCTG
GATGAAGCCATTGGTTTAGGTAAACATTTAAAAGGAGTGCTTGCCTATGCTGAAGATCCGTGTGGTGCTGAACAAGGTTA
TTCAAGTCGTGAAATCATGGCGGAATTTAAACGTGCAACTGGTTTACCAACAGCCACCAATATGATTGCAACAGACTGGC
GAGAAATGTCACATAGTATTCAACTTCAGGCGGTGGATATTCCGCTGGCCGACCCGCATTTCTGGACGCTTGAGGGTTCT
GTTCGTGTTTCTCAATTGTGCAATATGTACAATTTAACTTGGGGCTCACATTCAAATAATCATTTTGATGTCTCTTTGGC
CATGTTTACTCATGTGGCAGCAGCAGCAGTTGGAAATGTCACAGCAATTGATACACATTGGATCTGGCAAGAAGGTACAG
ATCATTTGACCAAACAGCCTCTTGAGATCAAGGGTGGAAAAATTCAGGTTCCAAGTGTGCCTGGTCTAGGTGTAGAACTG
GATTGGGATAATATCAATCGAGCCCACGAATTGTATAAAGCAAAAGGTTTGGGCGCACGTAATGATGCAGATGCTATGCA
ATTTATGGTGCCTAACTGGAAATTTGATCATAAAAAACCATGTCTTGTCCGTTAA

Upstream 100 bases:

>100_bases
CCAATGCACAAAAGAATAAGCGATTAACTTGACATGTTGTATGATGTCTTATAAGTTGTTTCATATCAAAGATAGATCAA
TAAGGCCAAGGAGTAACAAG

Downstream 100 bases:

>100_bases
CGATAAAATCTAAAAAGTGACGGTTATATTAGATATTCAGGATCTATTTATTTAGATCCTGTCAGCTATATTCAACAGAG
TCCTTAAATTATCTTGTAAA

Product: D-glucarate dehydratase

Products: NA

Alternate protein names: GDH; GlucD [H]

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MAASTPIIQSVRAIPVAGHDSMLLNLSGAHAPYFTRNLLVIEDNSGNIGVGEIPGGEKILATLNDAKSLILGQPIGEYKN
LLKKIHQTFADRDSGGRGNQTFDLRTTVHVVTAYESALLDLLGKHLNVNVASLLGDGQQRDEVEVLGYLFFIGDRKQTSL
DYATSTHLNHDWYQVRHEKALTPEAIQRLAEASYDRYGFKDFKLKGGVLHGEQEAEAVTAIARRFPDARVTLDPNGAWYL
DEAIGLGKHLKGVLAYAEDPCGAEQGYSSREIMAEFKRATGLPTATNMIATDWREMSHSIQLQAVDIPLADPHFWTLEGS
VRVSQLCNMYNLTWGSHSNNHFDVSLAMFTHVAAAAVGNVTAIDTHWIWQEGTDHLTKQPLEIKGGKIQVPSVPGLGVEL
DWDNINRAHELYKAKGLGARNDADAMQFMVPNWKFDHKKPCLVR

Sequences:

>Translated_444_residues
MAASTPIIQSVRAIPVAGHDSMLLNLSGAHAPYFTRNLLVIEDNSGNIGVGEIPGGEKILATLNDAKSLILGQPIGEYKN
LLKKIHQTFADRDSGGRGNQTFDLRTTVHVVTAYESALLDLLGKHLNVNVASLLGDGQQRDEVEVLGYLFFIGDRKQTSL
DYATSTHLNHDWYQVRHEKALTPEAIQRLAEASYDRYGFKDFKLKGGVLHGEQEAEAVTAIARRFPDARVTLDPNGAWYL
DEAIGLGKHLKGVLAYAEDPCGAEQGYSSREIMAEFKRATGLPTATNMIATDWREMSHSIQLQAVDIPLADPHFWTLEGS
VRVSQLCNMYNLTWGSHSNNHFDVSLAMFTHVAAAAVGNVTAIDTHWIWQEGTDHLTKQPLEIKGGKIQVPSVPGLGVEL
DWDNINRAHELYKAKGLGARNDADAMQFMVPNWKFDHKKPCLVR
>Mature_443_residues
AASTPIIQSVRAIPVAGHDSMLLNLSGAHAPYFTRNLLVIEDNSGNIGVGEIPGGEKILATLNDAKSLILGQPIGEYKNL
LKKIHQTFADRDSGGRGNQTFDLRTTVHVVTAYESALLDLLGKHLNVNVASLLGDGQQRDEVEVLGYLFFIGDRKQTSLD
YATSTHLNHDWYQVRHEKALTPEAIQRLAEASYDRYGFKDFKLKGGVLHGEQEAEAVTAIARRFPDARVTLDPNGAWYLD
EAIGLGKHLKGVLAYAEDPCGAEQGYSSREIMAEFKRATGLPTATNMIATDWREMSHSIQLQAVDIPLADPHFWTLEGSV
RVSQLCNMYNLTWGSHSNNHFDVSLAMFTHVAAAAVGNVTAIDTHWIWQEGTDHLTKQPLEIKGGKIQVPSVPGLGVELD
WDNINRAHELYKAKGLGARNDADAMQFMVPNWKFDHKKPCLVR

Specific function: Catalyzes the dehydration of glucarate to 5-keto-4- deoxy-D-glucarate (5-kdGluc). Also acts on L-idarate [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GlucD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789150, Length=441, Percent_Identity=69.8412698412698, Blast_Score=689, Evalue=0.0,
Organism=Escherichia coli, GI1789151, Length=443, Percent_Identity=63.882618510158, Blast_Score=578, Evalue=1e-166,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017653
- InterPro:   IPR013342
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE [H]

EC number: =4.2.1.40 [H]

Molecular weight: Translated: 49028; Mature: 48897

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAASTPIIQSVRAIPVAGHDSMLLNLSGAHAPYFTRNLLVIEDNSGNIGVGEIPGGEKIL
CCCCCCHHHHHHEEEECCCCCEEEEECCCCCCEEECEEEEEECCCCCEEECCCCCCCEEE
ATLNDAKSLILGQPIGEYKNLLKKIHQTFADRDSGGRGNQTFDLRTTVHVVTAYESALLD
EEHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEEEEHHHHHHHHH
LLGKHLNVNVASLLGDGQQRDEVEVLGYLFFIGDRKQTSLDYATSTHLNHDWYQVRHEKA
HHHHHCCCCHHHHHCCCCCCCHHHEEEEEEEECCCCCCCCCHHHCCCCCCHHHHHHHHCC
LTPEAIQRLAEASYDRYGFKDFKLKGGVLHGEQEAEAVTAIARRFPDARVTLDPNGAWYL
CCHHHHHHHHHCCCCCCCCEEEEEECCEECCCHHHHHHHHHHHHCCCCEEEECCCCCEEE
DEAIGLGKHLKGVLAYAEDPCGAEQGYSSREIMAEFKRATGLPTATNMIATDWREMSHSI
HHHHCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCEE
QLQAVDIPLADPHFWTLEGSVRVSQLCNMYNLTWGSHSNNHFDVSLAMFTHVAAAAVGNV
EEEEEECCCCCCCEEEEECCEEHHHHHHHHEEECCCCCCCEEEEEHHHHHHHHHHHHCCE
TAIDTHWIWQEGTDHLTKQPLEIKGGKIQVPSVPGLGVELDWDNINRAHELYKAKGLGAR
EEEEEEECCCCCCHHHHCCCCEECCCEEECCCCCCCCEEECCCCCHHHHHHHHHCCCCCC
NDADAMQFMVPNWKFDHKKPCLVR
CCCCHHEEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
AASTPIIQSVRAIPVAGHDSMLLNLSGAHAPYFTRNLLVIEDNSGNIGVGEIPGGEKIL
CCCCCHHHHHHEEEECCCCCEEEEECCCCCCEEECEEEEEECCCCCEEECCCCCCCEEE
ATLNDAKSLILGQPIGEYKNLLKKIHQTFADRDSGGRGNQTFDLRTTVHVVTAYESALLD
EEHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEEEEHHHHHHHHH
LLGKHLNVNVASLLGDGQQRDEVEVLGYLFFIGDRKQTSLDYATSTHLNHDWYQVRHEKA
HHHHHCCCCHHHHHCCCCCCCHHHEEEEEEEECCCCCCCCCHHHCCCCCCHHHHHHHHCC
LTPEAIQRLAEASYDRYGFKDFKLKGGVLHGEQEAEAVTAIARRFPDARVTLDPNGAWYL
CCHHHHHHHHHCCCCCCCCEEEEEECCEECCCHHHHHHHHHHHHCCCCEEEECCCCCEEE
DEAIGLGKHLKGVLAYAEDPCGAEQGYSSREIMAEFKRATGLPTATNMIATDWREMSHSI
HHHHCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCEE
QLQAVDIPLADPHFWTLEGSVRVSQLCNMYNLTWGSHSNNHFDVSLAMFTHVAAAAVGNV
EEEEEECCCCCCCEEEEECCEEHHHHHHHHEEECCCCCCCEEEEEHHHHHHHHHHHHCCE
TAIDTHWIWQEGTDHLTKQPLEIKGGKIQVPSVPGLGVELDWDNINRAHELYKAKGLGAR
EEEEEEECCCCCCHHHHCCCCEECCCEEECCCCCCCCEEECCCCCHHHHHHHHHCCCCCC
NDADAMQFMVPNWKFDHKKPCLVR
CCCCHHEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]