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The map label for this gene is ampD [H]

Identifier: 50083357

GI number: 50083357

Start: 60290

End: 60880

Strand: Direct

Name: ampD [H]

Synonym: ACIAD0063

Alternate gene names: 50083357

Gene position: 60290-60880 (Clockwise)

Preceding gene: 50083355

Following gene: 50083358

Centisome position: 1.68

GC content: 43.99

Gene sequence:

>591_bases
ATGAAATCACATTTTCAGGTTAAGGATGGCGTGCTGATTGGGGCTCAGCAATTGTCATCGCCAAATTATAATCAGCGTCC
TGAAAATACCGAAATTTGCCTGATCGTGGTGCATAACATTAGTTTGCCACCGTCGCAGTTTGGTGGTGGTTATATTCAAC
AGTTTTTCCAAAATCAGTTAGATTGGCGCTTGCATCCGTATTTTGAAACGATACAGGGCATGCAGGTGTCGGCACATTTA
TTGATTTTACGCTCGGGTGAAGTTATCCAGTTTGTCAATTTTCAGGATCGTGCATGGCATGCTGGGCGTTCGCAATATCT
TGGGCAGGTCGAATGTAACGACTACTCGATTGGTATTGAACTTGAAGGAAGTGACGATCTGCCTTTTGAAGATATTCAGT
ATGAACGCTTGAGCGAAGTGACGCAGCTACTGCAACACACCTATCCGAAAATTATGCAGCATCTGGCAGGCCATTCTGAT
ATCGCACCTGTGCGTAAAACTGATCCAGGCCCATTTTTTGACTGGCGTTATTTCCGCCGACTGCTCGACTCAAAAAAAAC
TGAATTTGGACATGATTTCGCAACGAAATAA

Upstream 100 bases:

>100_bases
ACAAAAGCAGATGCAATGCTGCTGTTCAAATGATATGTGTGGACTACACTTACATGTCAATGTACACAGGCCACAGTCGT
CAATGAGGGAATCAAGCATC

Downstream 100 bases:

>100_bases
TCTGCAACTTTCATTACAATATCGACTTTGTGTTTAGCCGTAGGTAGAGACGATGGCATTGTGGCGATCGACGTTTATTG
TCAGTGCAATGACGATGTTA

Product: N-acetyl-anhydromuranmyl-L-alanine amidase

Products: NA

Alternate protein names: N-acetylmuramoyl-L-alanine amidase [H]

Number of amino acids: Translated: 196; Mature: 196

Protein sequence:

>196_residues
MKSHFQVKDGVLIGAQQLSSPNYNQRPENTEICLIVVHNISLPPSQFGGGYIQQFFQNQLDWRLHPYFETIQGMQVSAHL
LILRSGEVIQFVNFQDRAWHAGRSQYLGQVECNDYSIGIELEGSDDLPFEDIQYERLSEVTQLLQHTYPKIMQHLAGHSD
IAPVRKTDPGPFFDWRYFRRLLDSKKTEFGHDFATK

Sequences:

>Translated_196_residues
MKSHFQVKDGVLIGAQQLSSPNYNQRPENTEICLIVVHNISLPPSQFGGGYIQQFFQNQLDWRLHPYFETIQGMQVSAHL
LILRSGEVIQFVNFQDRAWHAGRSQYLGQVECNDYSIGIELEGSDDLPFEDIQYERLSEVTQLLQHTYPKIMQHLAGHSD
IAPVRKTDPGPFFDWRYFRRLLDSKKTEFGHDFATK
>Mature_196_residues
MKSHFQVKDGVLIGAQQLSSPNYNQRPENTEICLIVVHNISLPPSQFGGGYIQQFFQNQLDWRLHPYFETIQGMQVSAHL
LILRSGEVIQFVNFQDRAWHAGRSQYLGQVECNDYSIGIELEGSDDLPFEDIQYERLSEVTQLLQHTYPKIMQHLAGHSD
IAPVRKTDPGPFFDWRYFRRLLDSKKTEFGHDFATK

Specific function: Involved in both cell wall peptidoglycans recycling and beta-lactamase induction. Specifically cleaves the amide bond between the lactyl group of N-acetylmuramic acid and the alpha- amino group of the L-alanine in degradation products containing an anhydr

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1786300, Length=181, Percent_Identity=51.9337016574586, Blast_Score=206, Evalue=1e-54,
Organism=Escherichia coli, GI1787092, Length=125, Percent_Identity=35.2, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 22730; Mature: 22730

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSHFQVKDGVLIGAQQLSSPNYNQRPENTEICLIVVHNISLPPSQFGGGYIQQFFQNQL
CCCCEECCCCEEEEHHHHCCCCCCCCCCCCCEEEEEEECCCCCCHHCCCHHHHHHHHHCC
DWRLHPYFETIQGMQVSAHLLILRSGEVIQFVNFQDRAWHAGRSQYLGQVECNDYSIGIE
CCEECHHHHHHCCCEEEEEEEEEECCCEEEEECCCCHHHHCCHHHCCCEEECCCEEEEEE
LEGSDDLPFEDIQYERLSEVTQLLQHTYPKIMQHLAGHSDIAPVRKTDPGPFFDWRYFRR
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHH
LLDSKKTEFGHDFATK
HHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKSHFQVKDGVLIGAQQLSSPNYNQRPENTEICLIVVHNISLPPSQFGGGYIQQFFQNQL
CCCCEECCCCEEEEHHHHCCCCCCCCCCCCCEEEEEEECCCCCCHHCCCHHHHHHHHHCC
DWRLHPYFETIQGMQVSAHLLILRSGEVIQFVNFQDRAWHAGRSQYLGQVECNDYSIGIE
CCEECHHHHHHCCCEEEEEEEEEECCCEEEEECCCCHHHHCCHHHCCCEEECCCEEEEEE
LEGSDDLPFEDIQYERLSEVTQLLQHTYPKIMQHLAGHSDIAPVRKTDPGPFFDWRYFRR
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHH
LLDSKKTEFGHDFATK
HHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2691840; 2607970; 9278503; 7959070 [H]