Click here to switch to the map view.

The map label for this gene is tyrS

Identifier: 50083309

GI number: 50083309

Start: 13646

End: 14860

Strand: Direct

Name: tyrS

Synonym: ACIAD0013

Alternate gene names: 50083309

Gene position: 13646-14860 (Clockwise)

Preceding gene: 50083307

Following gene: 50083310

Centisome position: 0.38

GC content: 45.35

Gene sequence:

>1215_bases
ATGTCAAATTTCTTGCCTGCTGAAGAACAGCTTGCCCTCATTCAACGAGGCACTCATGAAATTATTTCTGAAGAGGATCT
TTTAAAGAAACTTAAGGAAAATCGCCCACTGCGAATCAAAGCAGGGTTCGATCCAACTGCACCAGATTTGCATTTGGGTC
ATACGGTTCTGATTAATAAATTAAAAGCATTTCAAGATCTGGGCCACGAAGTCACGTTCTTGATTGGCGATTATACTGCC
ATGATTGGCGACCCAACTGGAAAAAGTGCGACGCGTCCGCCACTGACTCGTGAGCAGGTCGAGGCCAATGCCAAAACCTA
TCAAGAACAGGTCTTTAAAATTCTTGATCCAAATAAAACCAAGGTTCGCTTTAACTCGGAGTGGTTTAATCAGCGCACTG
CAGCAGATCTGATCCAGTTGGCCAGCCAGCAAACTGTTTCACGCATGCTGGAACGTGACGATTTCACCAAACGCTATAAC
AATCATCAGCCGATTGCCATTCATGAATTTTTATATCCTTTGGTGCAGGGCTATGACTCAATTGCACTTGAAGCCGATGT
AGAGCTGGGTGGAACCGATCAGACCTTTAACTTACTCATGGGGCGTACTCTGCAAGGACGCTACGGTCAAGAATCGCAAG
TGTGTATTACGGTGCCGATTCTAGAAGGTTTGGACGGTGTCAACAAAATGTCTAAATCACTGGGTAACTATATTGGGGTA
TTTGATGCGCCGGGTGCGATGTATCAAAAAGTCCTTTCCATGCCAGATACCTTAATCGAGCGCTATTTTGAACTACTCAG
CTTTAAATCACTTGACGAGATTCAGGGTTTACTCGATGAAATGGCCAATGGCCGTAACCCGCAAGATTTGAAAAAAATCT
TGGCGCTTGAACTGGTGGAGCGTTTCCACGATGCAGACGCTGCTGCCAATGCCCACAAAGGTGCAGGCAATATCATCACA
GAAGGTGAAATTCCTGAAGGCACACCAGAAGTGACGATTTCACGTGGCGAATTTGGTGGAGAAATCTTTATCGCTTCGAT
TGTGCGTTTGGCTGGACTCACCAAAAATGCTGCACAGGCCAAAGACGCTGTTTCACGTGGAGCCGTTAAAGTCGATTGGC
AGGTGGTGGATGCCAATTTCTCGGTGAAAGAAAATAAAACCTATCTGATTCAGGCAGGTAAAAAAGCCATTGCCCAAGTC
ACCTTTACTGATTAA

Upstream 100 bases:

>100_bases
CCCAATATAGATTGCTGTCATGAGCAACTTCGCCTGCAATATGCTGAAAAAGTGTTAGTATATCGCACAAATTGAATAAC
TGATGTGTTTAGGTTTTGTG

Downstream 100 bases:

>100_bases
TCAAACAATAAAAAAGCAGATCACGTGATCTGCTTTTTTATATCTAATCAGCGGTCTTTATTTTCCATGATAAACCTCAT
ATTTTTTTAGAAAAAATAAT

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS

Number of amino acids: Translated: 404; Mature: 403

Protein sequence:

>404_residues
MSNFLPAEEQLALIQRGTHEIISEEDLLKKLKENRPLRIKAGFDPTAPDLHLGHTVLINKLKAFQDLGHEVTFLIGDYTA
MIGDPTGKSATRPPLTREQVEANAKTYQEQVFKILDPNKTKVRFNSEWFNQRTAADLIQLASQQTVSRMLERDDFTKRYN
NHQPIAIHEFLYPLVQGYDSIALEADVELGGTDQTFNLLMGRTLQGRYGQESQVCITVPILEGLDGVNKMSKSLGNYIGV
FDAPGAMYQKVLSMPDTLIERYFELLSFKSLDEIQGLLDEMANGRNPQDLKKILALELVERFHDADAAANAHKGAGNIIT
EGEIPEGTPEVTISRGEFGGEIFIASIVRLAGLTKNAAQAKDAVSRGAVKVDWQVVDANFSVKENKTYLIQAGKKAIAQV
TFTD

Sequences:

>Translated_404_residues
MSNFLPAEEQLALIQRGTHEIISEEDLLKKLKENRPLRIKAGFDPTAPDLHLGHTVLINKLKAFQDLGHEVTFLIGDYTA
MIGDPTGKSATRPPLTREQVEANAKTYQEQVFKILDPNKTKVRFNSEWFNQRTAADLIQLASQQTVSRMLERDDFTKRYN
NHQPIAIHEFLYPLVQGYDSIALEADVELGGTDQTFNLLMGRTLQGRYGQESQVCITVPILEGLDGVNKMSKSLGNYIGV
FDAPGAMYQKVLSMPDTLIERYFELLSFKSLDEIQGLLDEMANGRNPQDLKKILALELVERFHDADAAANAHKGAGNIIT
EGEIPEGTPEVTISRGEFGGEIFIASIVRLAGLTKNAAQAKDAVSRGAVKVDWQVVDANFSVKENKTYLIQAGKKAIAQV
TFTD
>Mature_403_residues
SNFLPAEEQLALIQRGTHEIISEEDLLKKLKENRPLRIKAGFDPTAPDLHLGHTVLINKLKAFQDLGHEVTFLIGDYTAM
IGDPTGKSATRPPLTREQVEANAKTYQEQVFKILDPNKTKVRFNSEWFNQRTAADLIQLASQQTVSRMLERDDFTKRYNN
HQPIAIHEFLYPLVQGYDSIALEADVELGGTDQTFNLLMGRTLQGRYGQESQVCITVPILEGLDGVNKMSKSLGNYIGVF
DAPGAMYQKVLSMPDTLIERYFELLSFKSLDEIQGLLDEMANGRNPQDLKKILALELVERFHDADAAANAHKGAGNIITE
GEIPEGTPEVTISRGEFGGEIFIASIVRLAGLTKNAAQAKDAVSRGAVKVDWQVVDANFSVKENKTYLIQAGKKAIAQVT
FTD

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Homo sapiens, GI94681057, Length=404, Percent_Identity=26.2376237623762, Blast_Score=113, Evalue=4e-25,
Organism=Escherichia coli, GI1787925, Length=348, Percent_Identity=29.0229885057471, Blast_Score=134, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI25145802, Length=278, Percent_Identity=29.4964028776978, Blast_Score=96, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6325160, Length=420, Percent_Identity=24.047619047619, Blast_Score=94, Evalue=3e-20,
Organism=Drosophila melanogaster, GI20130383, Length=400, Percent_Identity=27, Blast_Score=127, Evalue=1e-29,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYY_ACIAD (Q6FG10)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_044819.1
- ProteinModelPortal:   Q6FG10
- SMR:   Q6FG10
- STRING:   Q6FG10
- GeneID:   2880799
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD0013
- NMPDR:   fig|62977.3.peg.1079
- eggNOG:   COG0162
- HOGENOM:   HBG288125
- OMA:   YVVQVGK
- PhylomeDB:   Q6FG10
- ProtClustDB:   PRK05912
- BioCyc:   ASP62977:ACIAD0013-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_02007
- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11766
- PRINTS:   PR01040
- SMART:   SM00363
- TIGRFAMs:   TIGR00234

Pfam domain/function: PF00579 tRNA-synt_1b

EC number: =6.1.1.1

Molecular weight: Translated: 44852; Mature: 44720

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50889 S4

Important sites: BINDING 232-232

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNFLPAEEQLALIQRGTHEIISEEDLLKKLKENRPLRIKAGFDPTAPDLHLGHTVLINK
CCCCCCHHHHHHHHHCCHHHHCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHH
LKAFQDLGHEVTFLIGDYTAMIGDPTGKSATRPPLTREQVEANAKTYQEQVFKILDPNKT
HHHHHHCCCEEEEEEECHHHEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
KVRFNSEWFNQRTAADLIQLASQQTVSRMLERDDFTKRYNNHQPIAIHEFLYPLVQGYDS
EEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHCCCCC
IALEADVELGGTDQTFNLLMGRTLQGRYGQESQVCITVPILEGLDGVNKMSKSLGNYIGV
EEEEEECCCCCCCHHHHHHHCCCCCCCCCCCCCEEEEEEHHHCCCHHHHHHHHHCCEEEC
FDAPGAMYQKVLSMPDTLIERYFELLSFKSLDEIQGLLDEMANGRNPQDLKKILALELVE
CCCCHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
RFHDADAAANAHKGAGNIITEGEIPEGTPEVTISRGEFGGEIFIASIVRLAGLTKNAAQA
HHHHHHHHCCCCCCCCCCEECCCCCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCHHHHH
KDAVSRGAVKVDWQVVDANFSVKENKTYLIQAGKKAIAQVTFTD
HHHHHCCCEEEEEEEEECCCEEECCCEEEEECCCHHEEEEEECC
>Mature Secondary Structure 
SNFLPAEEQLALIQRGTHEIISEEDLLKKLKENRPLRIKAGFDPTAPDLHLGHTVLINK
CCCCCHHHHHHHHHCCHHHHCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHH
LKAFQDLGHEVTFLIGDYTAMIGDPTGKSATRPPLTREQVEANAKTYQEQVFKILDPNKT
HHHHHHCCCEEEEEEECHHHEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
KVRFNSEWFNQRTAADLIQLASQQTVSRMLERDDFTKRYNNHQPIAIHEFLYPLVQGYDS
EEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHCCCCC
IALEADVELGGTDQTFNLLMGRTLQGRYGQESQVCITVPILEGLDGVNKMSKSLGNYIGV
EEEEEECCCCCCCHHHHHHHCCCCCCCCCCCCCEEEEEEHHHCCCHHHHHHHHHCCEEEC
FDAPGAMYQKVLSMPDTLIERYFELLSFKSLDEIQGLLDEMANGRNPQDLKKILALELVE
CCCCHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
RFHDADAAANAHKGAGNIITEGEIPEGTPEVTISRGEFGGEIFIASIVRLAGLTKNAAQA
HHHHHHHHCCCCCCCCCCEECCCCCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCHHHHH
KDAVSRGAVKVDWQVVDANFSVKENKTYLIQAGKKAIAQVTFTD
HHHHHCCCEEEEEEEEECCCEEECCCEEEEECCCHHEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA