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The map label for this gene is pykA

Identifier: 49481605

GI number: 49481605

Start: 3192434

End: 3193504

Strand: Reverse

Name: pykA

Synonym: BT9727_3123

Alternate gene names: 49481605

Gene position: 3193504-3192434 (Counterclockwise)

Preceding gene: 49478182

Following gene: 49481738

Centisome position: 60.97

GC content: 36.79

Gene sequence:

>1071_bases
ATGGTGATAAAAGTGACAATCGATCGAGTTTGTACAATTGGCCCAGCAAGTAATAATAAAGAAACGTTAGCACAGTTAAT
AAACAATGGTATGAAAATTGTTCGGCTAAATTTATCACATGGCACGCATGAAAGTCATAAAGATATTATTCGTTTAGTGA
AATCGTTAGATGATTCTATTAAAATTTTAGGCGATGTACAAGGTCCTAAAATAAGGTTAGGTGAAATAAAAGGAGAACAA
ATTACACTTCAGGCGGGAGATTCTTTTCTGTTGCATACGCAACCAGTTACAGGGAGTAGTACGGAAGCAAGTGTTGATTA
TGAAGGAATCGCGAATGATGTGAAAGTTGGAAGTAGAATTTTAATGAATGATGGTGAAGTTGAGTTAATCGTTGAGAAGG
TAAGTACGGATAAAATAGAAACAAAGGTCAAAACAGGTGGTAATATCTCATCTCATAAAGGGGTGAACTTACCAGGCGCA
ATCGTTAGTTTACCAGCTATTACAGAGAAAGATAAAAAAGATATTCAGTTTCTCTTAGAAGAGGGTGTTGATTTTATTGC
GTGTTCTTTCGTAAGGAAACCTAGTCATATAAAAGAAATACGAGATTTCATACAACAGTATAGAGAAACGTCACCTAATT
TAATTGCAAAAATAGAAACGATGGAAGCAATCGAGAATTTTCAAGATATATGTAAAGAAGCAGACGGAATTATGATTGCA
AGGGGCGATTTAGGAGTAGAGTTGCCGTTCCAATTTATTCCGCTCTTGCAAAAAATGATGATTCAGGAATGTAATCGAAC
GAATACATATGTTATTACAGCGACACAAATGCTTCAATCTATGGTAGATCATTCGATTCCAACAAGGGCTGAGGTAACTG
ATGTGTTTCAAGCTGTACTGGATGGAACGAATGCTGTTATGCTTTCTGCTGAAAGTGCTTCAGGAGAGCATCCGGTTGAA
AGTGTGAGTACACTACGTCTCGTTTCTGAATTTGCTGAGCATGTGAAACAAGACGGTCCTTTTGTGATGAAAGATGTACT
GGAATTGCTGCATAAATCTTTGGGTGAGTGA

Upstream 100 bases:

>100_bases
ATTAACAAAACTTTCGTAGTTTCAAAAGCCTACTTGTGTGAGTAGGCTTTTTTTACGTTGAGAGTGTAATTAAAGTTTAT
GGGAACCCTAATGAAAAAGG

Downstream 100 bases:

>100_bases
AAAATAAAAAGAGGGCGGATATGTATCCGTCCTCGTGTGTTTCATTCTACTCTTGCTCTTGCTCATCCAAAATCTTTTCA
AACGCAGCAGATACTTTTTC

Product: pyruvate kinase

Products: NA

Alternate protein names: PK 2 [H]

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MVIKVTIDRVCTIGPASNNKETLAQLINNGMKIVRLNLSHGTHESHKDIIRLVKSLDDSIKILGDVQGPKIRLGEIKGEQ
ITLQAGDSFLLHTQPVTGSSTEASVDYEGIANDVKVGSRILMNDGEVELIVEKVSTDKIETKVKTGGNISSHKGVNLPGA
IVSLPAITEKDKKDIQFLLEEGVDFIACSFVRKPSHIKEIRDFIQQYRETSPNLIAKIETMEAIENFQDICKEADGIMIA
RGDLGVELPFQFIPLLQKMMIQECNRTNTYVITATQMLQSMVDHSIPTRAEVTDVFQAVLDGTNAVMLSAESASGEHPVE
SVSTLRLVSEFAEHVKQDGPFVMKDVLELLHKSLGE

Sequences:

>Translated_356_residues
MVIKVTIDRVCTIGPASNNKETLAQLINNGMKIVRLNLSHGTHESHKDIIRLVKSLDDSIKILGDVQGPKIRLGEIKGEQ
ITLQAGDSFLLHTQPVTGSSTEASVDYEGIANDVKVGSRILMNDGEVELIVEKVSTDKIETKVKTGGNISSHKGVNLPGA
IVSLPAITEKDKKDIQFLLEEGVDFIACSFVRKPSHIKEIRDFIQQYRETSPNLIAKIETMEAIENFQDICKEADGIMIA
RGDLGVELPFQFIPLLQKMMIQECNRTNTYVITATQMLQSMVDHSIPTRAEVTDVFQAVLDGTNAVMLSAESASGEHPVE
SVSTLRLVSEFAEHVKQDGPFVMKDVLELLHKSLGE
>Mature_356_residues
MVIKVTIDRVCTIGPASNNKETLAQLINNGMKIVRLNLSHGTHESHKDIIRLVKSLDDSIKILGDVQGPKIRLGEIKGEQ
ITLQAGDSFLLHTQPVTGSSTEASVDYEGIANDVKVGSRILMNDGEVELIVEKVSTDKIETKVKTGGNISSHKGVNLPGA
IVSLPAITEKDKKDIQFLLEEGVDFIACSFVRKPSHIKEIRDFIQQYRETSPNLIAKIETMEAIENFQDICKEADGIMIA
RGDLGVELPFQFIPLLQKMMIQECNRTNTYVITATQMLQSMVDHSIPTRAEVTDVFQAVLDGTNAVMLSAESASGEHPVE
SVSTLRLVSEFAEHVKQDGPFVMKDVLELLHKSLGE

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PEP- utilizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=340, Percent_Identity=42.0588235294118, Blast_Score=271, Evalue=5e-73,
Organism=Homo sapiens, GI33286420, Length=340, Percent_Identity=42.0588235294118, Blast_Score=271, Evalue=5e-73,
Organism=Homo sapiens, GI33286418, Length=340, Percent_Identity=42.0588235294118, Blast_Score=271, Evalue=7e-73,
Organism=Homo sapiens, GI10835121, Length=340, Percent_Identity=41.4705882352941, Blast_Score=257, Evalue=1e-68,
Organism=Homo sapiens, GI32967597, Length=340, Percent_Identity=41.4705882352941, Blast_Score=257, Evalue=1e-68,
Organism=Homo sapiens, GI310128732, Length=283, Percent_Identity=40.2826855123675, Blast_Score=208, Evalue=6e-54,
Organism=Homo sapiens, GI310128730, Length=283, Percent_Identity=40.2826855123675, Blast_Score=208, Evalue=6e-54,
Organism=Homo sapiens, GI310128736, Length=231, Percent_Identity=37.6623376623377, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI310128734, Length=231, Percent_Identity=37.6623376623377, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI310128738, Length=215, Percent_Identity=35.8139534883721, Blast_Score=135, Evalue=8e-32,
Organism=Escherichia coli, GI1787965, Length=332, Percent_Identity=43.6746987951807, Blast_Score=286, Evalue=1e-78,
Organism=Escherichia coli, GI1788160, Length=335, Percent_Identity=40.8955223880597, Blast_Score=233, Evalue=1e-62,
Organism=Caenorhabditis elegans, GI17506829, Length=336, Percent_Identity=46.4285714285714, Blast_Score=280, Evalue=9e-76,
Organism=Caenorhabditis elegans, GI71984413, Length=336, Percent_Identity=46.4285714285714, Blast_Score=280, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI71984406, Length=336, Percent_Identity=46.4285714285714, Blast_Score=279, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI17506831, Length=336, Percent_Identity=46.4285714285714, Blast_Score=279, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI17544584, Length=350, Percent_Identity=41.1428571428571, Blast_Score=265, Evalue=4e-71,
Organism=Saccharomyces cerevisiae, GI6319279, Length=336, Percent_Identity=38.0952380952381, Blast_Score=223, Evalue=4e-59,
Organism=Saccharomyces cerevisiae, GI6324923, Length=334, Percent_Identity=35.9281437125749, Blast_Score=202, Evalue=5e-53,
Organism=Drosophila melanogaster, GI24648964, Length=340, Percent_Identity=43.2352941176471, Blast_Score=267, Evalue=1e-71,
Organism=Drosophila melanogaster, GI28571814, Length=340, Percent_Identity=43.2352941176471, Blast_Score=266, Evalue=1e-71,
Organism=Drosophila melanogaster, GI24648966, Length=322, Percent_Identity=39.7515527950311, Blast_Score=246, Evalue=3e-65,
Organism=Drosophila melanogaster, GI24581235, Length=339, Percent_Identity=33.9233038348083, Blast_Score=197, Evalue=8e-51,
Organism=Drosophila melanogaster, GI24646914, Length=182, Percent_Identity=39.5604395604396, Blast_Score=139, Evalue=3e-33,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 39159; Mature: 39159

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVIKVTIDRVCTIGPASNNKETLAQLINNGMKIVRLNLSHGTHESHKDIIRLVKSLDDSI
CEEEEEECCEEECCCCCCCHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHCCHHH
KILGDVQGPKIRLGEIKGEQITLQAGDSFLLHTQPVTGSSTEASVDYEGIANDVKVGSRI
EEHCCCCCCEEEEEECCCCEEEEEECCEEEEEECCCCCCCCCCCCCHHHCCHHHHHCCEE
LMNDGEVELIVEKVSTDKIETKVKTGGNISSHKGVNLPGAIVSLPAITEKDKKDIQFLLE
EECCCCEEEEEEHHCCHHHHHHHCCCCCCCCCCCCCCCHHHEECCCCCCCCHHHHHHHHH
EGVDFIACSFVRKPSHIKEIRDFIQQYRETSPNLIAKIETMEAIENFQDICKEADGIMIA
CCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEE
RGDLGVELPFQFIPLLQKMMIQECNRTNTYVITATQMLQSMVDHSIPTRAEVTDVFQAVL
ECCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHH
DGTNAVMLSAESASGEHPVESVSTLRLVSEFAEHVKQDGPFVMKDVLELLHKSLGE
CCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVIKVTIDRVCTIGPASNNKETLAQLINNGMKIVRLNLSHGTHESHKDIIRLVKSLDDSI
CEEEEEECCEEECCCCCCCHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHCCHHH
KILGDVQGPKIRLGEIKGEQITLQAGDSFLLHTQPVTGSSTEASVDYEGIANDVKVGSRI
EEHCCCCCCEEEEEECCCCEEEEEECCEEEEEECCCCCCCCCCCCCHHHCCHHHHHCCEE
LMNDGEVELIVEKVSTDKIETKVKTGGNISSHKGVNLPGAIVSLPAITEKDKKDIQFLLE
EECCCCEEEEEEHHCCHHHHHHHCCCCCCCCCCCCCCCHHHEECCCCCCCCHHHHHHHHH
EGVDFIACSFVRKPSHIKEIRDFIQQYRETSPNLIAKIETMEAIENFQDICKEADGIMIA
CCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEE
RGDLGVELPFQFIPLLQKMMIQECNRTNTYVITATQMLQSMVDHSIPTRAEVTDVFQAVL
ECCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHH
DGTNAVMLSAESASGEHPVESVSTLRLVSEFAEHVKQDGPFVMKDVLELLHKSLGE
CCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]