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The map label for this gene is nadB

Identifier: 49481520

GI number: 49481520

Start: 4226911

End: 4228440

Strand: Reverse

Name: nadB

Synonym: BT9727_4164

Alternate gene names: 49481520

Gene position: 4228440-4226911 (Counterclockwise)

Preceding gene: 49478853

Following gene: 49478633

Centisome position: 80.73

GC content: 39.35

Gene sequence:

>1530_bases
ATGCCAAGTGCAGATGTCTTAATTATTGGAAGTGGTGTTGCGGCACTTCGTGTTGCAAAAGAGATTTGTCACGAAAAGAA
TGTGATTGTTATCACAAAGGAAACGAATCGTAATAATAATACACATTTAGCGCAAGGTGGAATTGCCGCAGCGGTTGCTA
CATATGACGATCCGAATGATCATTTTGAAGATACATTAGTAGCAGGATGTCGTTATAACAATGAAGATGTGGTCCGTTAT
TTAGTTGAAGAAGGACCGAAAGAAATGAAGAATCTCATTGAAAATGGAATGCAATTTGATGGAGATGAAACGGGTCCCCA
TCTTGGAAAAGAAGGTGCACATCGAAAGCGTCGTATTTTACATGCAGGTGGCGATGCAACAGGAAAGAATTTATTAGAGC
ATTTCATTCAAGAAGTAGTTCCGCATGTTACGGTAGTGGAACAGGAAATGGTGCTTGATTTTATTATAGAAAGTGAAAAA
TGTGTTGGAGCTTTAACGCGAGATAGTAAAGGGAAGTTGAAGCGTTATGATGCAGATTATACTGTGCTAGCCTCAGGCGG
GGTTGGTGGTTTATACGCCTTTACTTCTAATGATAAAACAATCACGGGTGATGGACTTGCAATGGTATACCGCGCAGGTG
GGGAGCTCGTAGATTTAGAGTTTGTACAATTTCATCCAACAATGTTATATGCGGGCGGGCGATGCTGTGGCCTCGTTTCT
GAAGCTGTCCGTGGGGAAGGAGCTGTCCTTATAAATGGAAAAGGGCAGCGTTTTATGATGGATATACACGATGATTACGA
TCTCGCACCGCGTGATGTGGTGGCAAGAGCGATTCATGAACAGCTTTTGTCGGGTGAAAAAGTGTATTTGAATATTGAGT
CTATCCAAAATTTCCAAGAACGTTTTCCAACTGTATCAGCATTGTGTAAAAAGAATGGTGTTGATATTACTAAAAAACGC
ATTCCGGTCGTACCCGGAGCTCATTTTCATATGGGCGGTGTGAAAACAAACATTGATGGGGAGACGTCTATTCCAAATTT
ATATGCAGTCGGTGAAGTAGCTTGTAATGGTGTTCATGGAGCAAATAGATTAGCGAGTAATTCGTTATTAGAAGGTCTTG
TGTTTGGAAAAAGAATAGGACAACATATTTTAACGAAAGCGACAAAAGACAAATTGAACGACTTCGCTGAGAAGGAAAAG
AAATTTATCGTTCTAAATCATTTGCCGACGAAAGAAGAAATACAAGAATGCATGATGAAATATGTTGGGATTGTACGAAC
AGAGCAAAGTTTATCTTATGCGAAGAGGTGGCTTAGTAAATACGGTGTTCGAAATATGATATTACAACATGATGCTCTTA
CAAATGAAGAGATAACGCTTATTAATATGTTAACAGTTTGTGAGCTTATCGTTGTATCAGCTTTGCAAAGAGAAGAGAGT
ATTGGTGGTCATTATCGAAGTGATTATCCACATAGAAATAGCGCACAAAAAGAGATCATTCGAGTGAAAAGAAAACTACA
ACTTGTGTAA

Upstream 100 bases:

>100_bases
GTGAAAATAAGAATTATAAAAAAACTCTTGTCATTATTTTAAAACTATGTAAATATAGGTGTCAAGACAGTTGAAAAGTG
TAAAGTAGGAGGCAACAATT

Downstream 100 bases:

>100_bases
TGAGGGGAAGAGGGGTTTTATGAATACGATAAAGGTTAAAGAAGCATTAAATCGATTTTTTCTAGAAGATATAGGAGAAA
GAGATGTAACATCTCAGCTT

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 509; Mature: 508

Protein sequence:

>509_residues
MPSADVLIIGSGVAALRVAKEICHEKNVIVITKETNRNNNTHLAQGGIAAAVATYDDPNDHFEDTLVAGCRYNNEDVVRY
LVEEGPKEMKNLIENGMQFDGDETGPHLGKEGAHRKRRILHAGGDATGKNLLEHFIQEVVPHVTVVEQEMVLDFIIESEK
CVGALTRDSKGKLKRYDADYTVLASGGVGGLYAFTSNDKTITGDGLAMVYRAGGELVDLEFVQFHPTMLYAGGRCCGLVS
EAVRGEGAVLINGKGQRFMMDIHDDYDLAPRDVVARAIHEQLLSGEKVYLNIESIQNFQERFPTVSALCKKNGVDITKKR
IPVVPGAHFHMGGVKTNIDGETSIPNLYAVGEVACNGVHGANRLASNSLLEGLVFGKRIGQHILTKATKDKLNDFAEKEK
KFIVLNHLPTKEEIQECMMKYVGIVRTEQSLSYAKRWLSKYGVRNMILQHDALTNEEITLINMLTVCELIVVSALQREES
IGGHYRSDYPHRNSAQKEIIRVKRKLQLV

Sequences:

>Translated_509_residues
MPSADVLIIGSGVAALRVAKEICHEKNVIVITKETNRNNNTHLAQGGIAAAVATYDDPNDHFEDTLVAGCRYNNEDVVRY
LVEEGPKEMKNLIENGMQFDGDETGPHLGKEGAHRKRRILHAGGDATGKNLLEHFIQEVVPHVTVVEQEMVLDFIIESEK
CVGALTRDSKGKLKRYDADYTVLASGGVGGLYAFTSNDKTITGDGLAMVYRAGGELVDLEFVQFHPTMLYAGGRCCGLVS
EAVRGEGAVLINGKGQRFMMDIHDDYDLAPRDVVARAIHEQLLSGEKVYLNIESIQNFQERFPTVSALCKKNGVDITKKR
IPVVPGAHFHMGGVKTNIDGETSIPNLYAVGEVACNGVHGANRLASNSLLEGLVFGKRIGQHILTKATKDKLNDFAEKEK
KFIVLNHLPTKEEIQECMMKYVGIVRTEQSLSYAKRWLSKYGVRNMILQHDALTNEEITLINMLTVCELIVVSALQREES
IGGHYRSDYPHRNSAQKEIIRVKRKLQLV
>Mature_508_residues
PSADVLIIGSGVAALRVAKEICHEKNVIVITKETNRNNNTHLAQGGIAAAVATYDDPNDHFEDTLVAGCRYNNEDVVRYL
VEEGPKEMKNLIENGMQFDGDETGPHLGKEGAHRKRRILHAGGDATGKNLLEHFIQEVVPHVTVVEQEMVLDFIIESEKC
VGALTRDSKGKLKRYDADYTVLASGGVGGLYAFTSNDKTITGDGLAMVYRAGGELVDLEFVQFHPTMLYAGGRCCGLVSE
AVRGEGAVLINGKGQRFMMDIHDDYDLAPRDVVARAIHEQLLSGEKVYLNIESIQNFQERFPTVSALCKKNGVDITKKRI
PVVPGAHFHMGGVKTNIDGETSIPNLYAVGEVACNGVHGANRLASNSLLEGLVFGKRIGQHILTKATKDKLNDFAEKEKK
FIVLNHLPTKEEIQECMMKYVGIVRTEQSLSYAKRWLSKYGVRNMILQHDALTNEEITLINMLTVCELIVVSALQREESI
GGHYRSDYPHRNSAQKEIIRVKRKLQLV

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=524, Percent_Identity=33.206106870229, Blast_Score=213, Evalue=4e-55,
Organism=Escherichia coli, GI1788928, Length=516, Percent_Identity=36.6279069767442, Blast_Score=296, Evalue=3e-81,
Organism=Escherichia coli, GI1790597, Length=511, Percent_Identity=31.7025440313112, Blast_Score=201, Evalue=6e-53,
Organism=Escherichia coli, GI1786942, Length=522, Percent_Identity=30.2681992337165, Blast_Score=186, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI17550100, Length=522, Percent_Identity=31.8007662835249, Blast_Score=211, Evalue=5e-55,
Organism=Caenorhabditis elegans, GI17505833, Length=524, Percent_Identity=31.6793893129771, Blast_Score=211, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI71986328, Length=462, Percent_Identity=25.7575757575758, Blast_Score=94, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6322701, Length=528, Percent_Identity=33.5227272727273, Blast_Score=226, Evalue=5e-60,
Organism=Saccharomyces cerevisiae, GI6322416, Length=515, Percent_Identity=33.3980582524272, Blast_Score=218, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6320788, Length=479, Percent_Identity=26.3048016701461, Blast_Score=103, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6322511, Length=473, Percent_Identity=25.5813953488372, Blast_Score=88, Evalue=3e-18,
Organism=Drosophila melanogaster, GI17137288, Length=522, Percent_Identity=34.0996168582375, Blast_Score=216, Evalue=4e-56,
Organism=Drosophila melanogaster, GI24655642, Length=522, Percent_Identity=34.0996168582375, Blast_Score=216, Evalue=4e-56,
Organism=Drosophila melanogaster, GI24655647, Length=522, Percent_Identity=34.0996168582375, Blast_Score=216, Evalue=4e-56,
Organism=Drosophila melanogaster, GI24663005, Length=523, Percent_Identity=31.1663479923518, Blast_Score=204, Evalue=1e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 56323; Mature: 56192

Theoretical pI: Translated: 6.82; Mature: 6.82

Prosite motif: PS00079 MULTICOPPER_OXIDASE1 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSADVLIIGSGVAALRVAKEICHEKNVIVITKETNRNNNTHLAQGGIAAAVATYDDPND
CCCCCEEEEECCHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCCEEEEEEECCCCCC
HFEDTLVAGCRYNNEDVVRYLVEEGPKEMKNLIENGMQFDGDETGPHLGKEGAHRKRRIL
HHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHE
HAGGDATGKNLLEHFIQEVVPHVTVVEQEMVLDFIIESEKCVGALTRDSKGKLKRYDADY
ECCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCEEECCCE
TVLASGGVGGLYAFTSNDKTITGDGLAMVYRAGGELVDLEFVQFHPTMLYAGGRCCGLVS
EEEECCCCCEEEEEECCCCEECCCCEEEEEECCCCEEEEEEEEECCEEEECCCCHHHHHH
EAVRGEGAVLINGKGQRFMMDIHDDYDLAPRDVVARAIHEQLLSGEKVYLNIESIQNFQE
HHHCCCCEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHH
RFPTVSALCKKNGVDITKKRIPVVPGAHFHMGGVKTNIDGETSIPNLYAVGEVACNGVHG
HCCHHHHHHHCCCCCCHHHCCCCCCCCCEECCCEEECCCCCCCCCCEEEEHHHHHCCCCH
ANRLASNSLLEGLVFGKRIGQHILTKATKDKLNDFAEKEKKFIVLNHLPTKEEIQECMMK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHH
YVGIVRTEQSLSYAKRWLSKYGVRNMILQHDALTNEEITLINMLTVCELIVVSALQREES
HHHHHCCHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IGGHYRSDYPHRNSAQKEIIRVKRKLQLV
CCCCCCCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PSADVLIIGSGVAALRVAKEICHEKNVIVITKETNRNNNTHLAQGGIAAAVATYDDPND
CCCCEEEEECCHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCCEEEEEEECCCCCC
HFEDTLVAGCRYNNEDVVRYLVEEGPKEMKNLIENGMQFDGDETGPHLGKEGAHRKRRIL
HHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHE
HAGGDATGKNLLEHFIQEVVPHVTVVEQEMVLDFIIESEKCVGALTRDSKGKLKRYDADY
ECCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCEEECCCE
TVLASGGVGGLYAFTSNDKTITGDGLAMVYRAGGELVDLEFVQFHPTMLYAGGRCCGLVS
EEEECCCCCEEEEEECCCCEECCCCEEEEEECCCCEEEEEEEEECCEEEECCCCHHHHHH
EAVRGEGAVLINGKGQRFMMDIHDDYDLAPRDVVARAIHEQLLSGEKVYLNIESIQNFQE
HHHCCCCEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHH
RFPTVSALCKKNGVDITKKRIPVVPGAHFHMGGVKTNIDGETSIPNLYAVGEVACNGVHG
HCCHHHHHHHCCCCCCHHHCCCCCCCCCEECCCEEECCCCCCCCCCEEEEHHHHHCCCCH
ANRLASNSLLEGLVFGKRIGQHILTKATKDKLNDFAEKEKKFIVLNHLPTKEEIQECMMK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHH
YVGIVRTEQSLSYAKRWLSKYGVRNMILQHDALTNEEITLINMLTVCELIVVSALQREES
HHHHHCCHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IGGHYRSDYPHRNSAQKEIIRVKRKLQLV
CCCCCCCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8444804; 9384377 [H]