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The map label for this gene is ykvZ [H]

Identifier: 49481301

GI number: 49481301

Start: 5143157

End: 5144134

Strand: Reverse

Name: ykvZ [H]

Synonym: BT9727_5083

Alternate gene names: 49481301

Gene position: 5144134-5143157 (Counterclockwise)

Preceding gene: 49481300

Following gene: 49481305

Centisome position: 98.21

GC content: 34.05

Gene sequence:

>978_bases
ATGACGAATATAAGAAAGATTGCTGAACTTGCTGGGGTATCTGTTTCAACTGTTTCACGTGTACTAAATAATCATCCTTA
TGTGAATGAACAGAAACGAAAGGAAATTTTAGCAATTATAGAGGAATTAAATTATACGCAAAATGTGAATGCAATTCATT
TAGTGAAAGGAAAAACGAATGTAATTGGCGTTTTATTACCACACGTAAATGATCAATATTATAGCGCTATTATTGAAGGC
ATATCGAAGGAAACAGCAAAGAACAGTTATAATATGATGCTTTGTCAGACGAATTATAGTGAGGAAAGAGAATTAGAAAT
TTTGAATATGTTAAAAATGAAAAAGCTTGATGGGGTTATTATATGTTCCCGGACAAATGGTAAGGAGAAGCTTGAAGAAT
ATACGAAATTTGGTCCAATCGTTATGTGTGAGGAAATAGATTCAAAATACATTTCAAGTGTACATGTTGATTACTATAAA
GTATTTTCACAAGGGATGAAAAACTTAATAGATGCCGGTCATACACGTATTGGGTATTGTATTGGAAGAAGTAATAGCAT
TAATAGCCAAAGAAGAAAACAAGCATATGAAGATTCATTTCAACAGATTGCTGTAACACCGTTAGAGACCTGGAAGTTTA
AAGGCTGTTTTACGGTGGAAGACGGACGTAATGTAATTAGGGAATGGGCCTGTATGTCAGTAAAGCCAACTGCGTTTCTT
GTATCTTGTAACCATATTGCAGCGGGAATGGTAACGGAGGCGAAAAAACAAGGAATTCGTATTCCAGAAGATATTACGAT
TATCGGTTGTGATGATCAAGATGTAGCAGACATTTTAGGTATTACGACGATTTCATATTCTAGTAAAAATGTTGGCGTGA
AGGCATTTGAATTATTATATGAAAAGATTAATGATGAGCAAATAGATGTAAAGCACGTGGAGTTATTGCCAGAATTCGTA
AAAAGGGAAACGACATAA

Upstream 100 bases:

>100_bases
ATAGTTGTATATCCTTTCAAAATGTATTTGCTAGGAAAGGATATATTGTGGAAAGCTTACCAGGTCAAGGGAGAATTTTT
GCAGGGAAGGGGAAAATGAG

Downstream 100 bases:

>100_bases
TAGATAAGGTGTGAAAATCGCTTTTCAAAATTGCGTTTGGTATAATTATGGACAATCTATGAGGGCTAAAAGGGGTTTTG
ATATGAGATCAAAAGTAGTA

Product: LacI family sugar-binding transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MTNIRKIAELAGVSVSTVSRVLNNHPYVNEQKRKEILAIIEELNYTQNVNAIHLVKGKTNVIGVLLPHVNDQYYSAIIEG
ISKETAKNSYNMMLCQTNYSEERELEILNMLKMKKLDGVIICSRTNGKEKLEEYTKFGPIVMCEEIDSKYISSVHVDYYK
VFSQGMKNLIDAGHTRIGYCIGRSNSINSQRRKQAYEDSFQQIAVTPLETWKFKGCFTVEDGRNVIREWACMSVKPTAFL
VSCNHIAAGMVTEAKKQGIRIPEDITIIGCDDQDVADILGITTISYSSKNVGVKAFELLYEKINDEQIDVKHVELLPEFV
KRETT

Sequences:

>Translated_325_residues
MTNIRKIAELAGVSVSTVSRVLNNHPYVNEQKRKEILAIIEELNYTQNVNAIHLVKGKTNVIGVLLPHVNDQYYSAIIEG
ISKETAKNSYNMMLCQTNYSEERELEILNMLKMKKLDGVIICSRTNGKEKLEEYTKFGPIVMCEEIDSKYISSVHVDYYK
VFSQGMKNLIDAGHTRIGYCIGRSNSINSQRRKQAYEDSFQQIAVTPLETWKFKGCFTVEDGRNVIREWACMSVKPTAFL
VSCNHIAAGMVTEAKKQGIRIPEDITIIGCDDQDVADILGITTISYSSKNVGVKAFELLYEKINDEQIDVKHVELLPEFV
KRETT
>Mature_324_residues
TNIRKIAELAGVSVSTVSRVLNNHPYVNEQKRKEILAIIEELNYTQNVNAIHLVKGKTNVIGVLLPHVNDQYYSAIIEGI
SKETAKNSYNMMLCQTNYSEERELEILNMLKMKKLDGVIICSRTNGKEKLEEYTKFGPIVMCEEIDSKYISSVHVDYYKV
FSQGMKNLIDAGHTRIGYCIGRSNSINSQRRKQAYEDSFQQIAVTPLETWKFKGCFTVEDGRNVIREWACMSVKPTAFLV
SCNHIAAGMVTEAKKQGIRIPEDITIIGCDDQDVADILGITTISYSSKNVGVKAFELLYEKINDEQIDVKHVELLPEFVK
RETT

Specific function: Transcriptional Repressor For The Ribose Rbsdacbk Operon. Rbsr Binds To A Region Of Perfect Dyad Symmetry Spanning The Rbs Operon Transcriptional Start Site. The Affinity For The Rbs Operator Is Reduced By Addition Of Ribose, Consistent With Ribose Being

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790194, Length=331, Percent_Identity=26.8882175226586, Blast_Score=137, Evalue=8e-34,
Organism=Escherichia coli, GI1787948, Length=330, Percent_Identity=26.3636363636364, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1790369, Length=311, Percent_Identity=25.4019292604502, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1788474, Length=329, Percent_Identity=26.1398176291793, Blast_Score=124, Evalue=7e-30,
Organism=Escherichia coli, GI1789202, Length=285, Percent_Identity=25.6140350877193, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI48994940, Length=317, Percent_Identity=24.9211356466877, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1789068, Length=281, Percent_Identity=24.5551601423488, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI1789456, Length=320, Percent_Identity=26.25, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1786540, Length=326, Percent_Identity=24.8466257668712, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1787580, Length=314, Percent_Identity=24.203821656051, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1790715, Length=322, Percent_Identity=23.2919254658385, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787906, Length=197, Percent_Identity=25.3807106598985, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1786268, Length=316, Percent_Identity=18.3544303797468, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1790689, Length=195, Percent_Identity=24.6153846153846, Blast_Score=71, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36851; Mature: 36720

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNIRKIAELAGVSVSTVSRVLNNHPYVNEQKRKEILAIIEELNYTQNVNAIHLVKGKTN
CCHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCC
VIGVLLPHVNDQYYSAIIEGISKETAKNSYNMMLCQTNYSEERELEILNMLKMKKLDGVI
EEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCEE
ICSRTNGKEKLEEYTKFGPIVMCEEIDSKYISSVHVDYYKVFSQGMKNLIDAGHTRIGYC
EEECCCCHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
IGRSNSINSQRRKQAYEDSFQQIAVTPLETWKFKGCFTVEDGRNVIREWACMSVKPTAFL
EECCCCCCHHHHHHHHHHHHHHEECCCHHHEEECCEEEECCHHHHHHHHHHCCCCCCEEE
VSCNHIAAGMVTEAKKQGIRIPEDITIIGCDDQDVADILGITTISYSSKNVGVKAFELLY
EECCHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHEEEECCCCCCHHHHHHHH
EKINDEQIDVKHVELLPEFVKRETT
HHHCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TNIRKIAELAGVSVSTVSRVLNNHPYVNEQKRKEILAIIEELNYTQNVNAIHLVKGKTN
CHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCC
VIGVLLPHVNDQYYSAIIEGISKETAKNSYNMMLCQTNYSEERELEILNMLKMKKLDGVI
EEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCEE
ICSRTNGKEKLEEYTKFGPIVMCEEIDSKYISSVHVDYYKVFSQGMKNLIDAGHTRIGYC
EEECCCCHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
IGRSNSINSQRRKQAYEDSFQQIAVTPLETWKFKGCFTVEDGRNVIREWACMSVKPTAFL
EECCCCCCHHHHHHHHHHHHHHEECCCHHHEEECCEEEECCHHHHHHHHHHCCCCCCEEE
VSCNHIAAGMVTEAKKQGIRIPEDITIIGCDDQDVADILGITTISYSSKNVGVKAFELLY
EECCHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHEEEECCCCCCHHHHHHHH
EKINDEQIDVKHVELLPEFVKRETT
HHHCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]