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The map label for this gene is emrB

Identifier: 49480683

GI number: 49480683

Start: 4365331

End: 4367130

Strand: Reverse

Name: emrB

Synonym: BT9727_4299

Alternate gene names: 49480683

Gene position: 4367130-4365331 (Counterclockwise)

Preceding gene: 49480684

Following gene: 49478690

Centisome position: 83.38

GC content: 37.22

Gene sequence:

>1800_bases
ATGTCCTCAATCGTAATTGCAGGATATGCACTATTTTGTATAATCGTCTTCTTCCTTGTAAATCGTCTTTTACGAAAGAA
AAAAACGAAAATGGGAGAAGAACAAGTCCCAGCCGTAAGTAAAATAGAAGTAAGTAAGGTAGAAACAGAAGAGAAAGACA
TTGAACACAAGCAAGAACCAGAAATTTCTAATGTAGTTGAGTTAGAAACAGGAAAGCAAGAAGAAGTAAAACAAGAAAAA
GAAGTGCCGAAAAAACAAATGTCTATGCCTGTAGAGAATGTGAACGTAAAAGCAGTTGTAGCTGTATTAATTCTCGGCAT
GTTCGTTTCTATTTTAAACCAAACAATCATTAACGTTGCATTGCCTCCGTTAATGAATGAATTTAACGTATCAACTTCAA
CAGCACAATGGTTAATTACAGGTTTCATGCTTGTAAATGGTATTTTAGTACCGATTAGTGCCTTTTTAGTTTCACGATTT
ACGTATCGTAAATTATTCGTAGCAGCAATGTTATTCTTCACGGTAGGATCTATCATTTGTGCTACTTCAGGAAACTTTAC
AATGATGATGACAGGGCGCGTAATTCAAGCGGTTGGTGCGGGTATTTTAATGCCGGTTGGTATGAATATCTTCATGACAC
TATTCCCGCCTCATAAGCGCGGAGCAGCGATGGGATTATTAGGTGTAGCAATGATTTTAGCACCAGCTATCGGACCAACT
GTAACAGGTTGGGTGATTGAAAACTATAGCTGGAACTTAATGTTCTATGCGATGTTTATTATTGGTTTAATTATTACGTT
CCTGTCTTTAAAATTCTTTACACTAGCTCAGCCAGTGTCTAACACGAAATTAGATGTATTCGGTGTTGTTAGTTCAAGTA
TTGGACTAGGAAGCTTATTGTACGGATTTAGTGAAGCTGGAAATAATAGTTGGACTAGTGCAGAAGTTGTTATTTCACTT
ATTATCGGTGTAATTGGTTTAGCGTTATTTATTTGGAGAGAGTTAACAACGGATAATAAAATGCTTGATTTACAAGTATT
TAAATACCCGGTATTTACTTTTACTTTAGTAATTAATGCAATCGTTACGATGGCATTATTCGGAGGTATGTTATTACTTC
CTGTATATCTGCAAAACATTCGTGGCTTCACGCCAATTGAATCTGGTTTACTACTACTTCCAGGATCATTAATTATGGGA
ATCATGGGACCAGTAGCGGGTAAATTATTCGATAAATATGGCATTCGTCCATTAGCGATAATTGGATTGGCAATTACGAC
GTATGCGACATATGAATTTACAAAATTATCGATGGATACACCGTATAGCGTTATTATGACGGATTATATTATACGCTCAA
TTGGTATGTCATTCATTATGATGCCAATTATGACAGCTGGTATGAACGCGCTACCGATGAAATTAATTTCTCACGGTACA
GCAACGCAAAATACGTCAAGACAAGTAGCTGGTTCAATTGGAACAGCGATATTAATCACGCTTATGACGCAACAAACGAC
TGCTCACGTAGCAGATTATGGAAACATGTTAACAACGTCAAATCCAATTTTAGTTGATAAAGTGCATGGCATGGGGCAAA
GCTTAGCGGCCTTAGCCGGCTCAGCGCAAGCAGGAGATGCGATGAGCACACAGTTATTATTTGGACAAATTTCGAAGTTA
TCTGCAATTAACGGTATTAACGATGCTTTCTTAATTGCGACGATACTCGCAGGGATTGCTTGGGTGTTATCGTTCTTCTT
ACCATCAGGCAATAAACCAAATAGAAAAGCAGGGAATTAA

Upstream 100 bases:

>100_bases
AGTAGTACCAGTTAAAATTTCTTTAGATCATAGCAAATCAGTAAATATTGTTCCTGGCATGAATGTGACAGTTCGTATTC
ATAAGTAAGGGGGAGATGAG

Downstream 100 bases:

>100_bases
TTTCCCTGCTTTTTATTTTGAATAGTATTAATAAATAAATGAGAAATAACGATCCAAATTATTGAAAAATGTTAAAGAAA
GAGGAATTTGAAGTTGTTAT

Product: multidrug resistance protein B

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 599; Mature: 598

Protein sequence:

>599_residues
MSSIVIAGYALFCIIVFFLVNRLLRKKKTKMGEEQVPAVSKIEVSKVETEEKDIEHKQEPEISNVVELETGKQEEVKQEK
EVPKKQMSMPVENVNVKAVVAVLILGMFVSILNQTIINVALPPLMNEFNVSTSTAQWLITGFMLVNGILVPISAFLVSRF
TYRKLFVAAMLFFTVGSIICATSGNFTMMMTGRVIQAVGAGILMPVGMNIFMTLFPPHKRGAAMGLLGVAMILAPAIGPT
VTGWVIENYSWNLMFYAMFIIGLIITFLSLKFFTLAQPVSNTKLDVFGVVSSSIGLGSLLYGFSEAGNNSWTSAEVVISL
IIGVIGLALFIWRELTTDNKMLDLQVFKYPVFTFTLVINAIVTMALFGGMLLLPVYLQNIRGFTPIESGLLLLPGSLIMG
IMGPVAGKLFDKYGIRPLAIIGLAITTYATYEFTKLSMDTPYSVIMTDYIIRSIGMSFIMMPIMTAGMNALPMKLISHGT
ATQNTSRQVAGSIGTAILITLMTQQTTAHVADYGNMLTTSNPILVDKVHGMGQSLAALAGSAQAGDAMSTQLLFGQISKL
SAINGINDAFLIATILAGIAWVLSFFLPSGNKPNRKAGN

Sequences:

>Translated_599_residues
MSSIVIAGYALFCIIVFFLVNRLLRKKKTKMGEEQVPAVSKIEVSKVETEEKDIEHKQEPEISNVVELETGKQEEVKQEK
EVPKKQMSMPVENVNVKAVVAVLILGMFVSILNQTIINVALPPLMNEFNVSTSTAQWLITGFMLVNGILVPISAFLVSRF
TYRKLFVAAMLFFTVGSIICATSGNFTMMMTGRVIQAVGAGILMPVGMNIFMTLFPPHKRGAAMGLLGVAMILAPAIGPT
VTGWVIENYSWNLMFYAMFIIGLIITFLSLKFFTLAQPVSNTKLDVFGVVSSSIGLGSLLYGFSEAGNNSWTSAEVVISL
IIGVIGLALFIWRELTTDNKMLDLQVFKYPVFTFTLVINAIVTMALFGGMLLLPVYLQNIRGFTPIESGLLLLPGSLIMG
IMGPVAGKLFDKYGIRPLAIIGLAITTYATYEFTKLSMDTPYSVIMTDYIIRSIGMSFIMMPIMTAGMNALPMKLISHGT
ATQNTSRQVAGSIGTAILITLMTQQTTAHVADYGNMLTTSNPILVDKVHGMGQSLAALAGSAQAGDAMSTQLLFGQISKL
SAINGINDAFLIATILAGIAWVLSFFLPSGNKPNRKAGN
>Mature_598_residues
SSIVIAGYALFCIIVFFLVNRLLRKKKTKMGEEQVPAVSKIEVSKVETEEKDIEHKQEPEISNVVELETGKQEEVKQEKE
VPKKQMSMPVENVNVKAVVAVLILGMFVSILNQTIINVALPPLMNEFNVSTSTAQWLITGFMLVNGILVPISAFLVSRFT
YRKLFVAAMLFFTVGSIICATSGNFTMMMTGRVIQAVGAGILMPVGMNIFMTLFPPHKRGAAMGLLGVAMILAPAIGPTV
TGWVIENYSWNLMFYAMFIIGLIITFLSLKFFTLAQPVSNTKLDVFGVVSSSIGLGSLLYGFSEAGNNSWTSAEVVISLI
IGVIGLALFIWRELTTDNKMLDLQVFKYPVFTFTLVINAIVTMALFGGMLLLPVYLQNIRGFTPIESGLLLLPGSLIMGI
MGPVAGKLFDKYGIRPLAIIGLAITTYATYEFTKLSMDTPYSVIMTDYIIRSIGMSFIMMPIMTAGMNALPMKLISHGTA
TQNTSRQVAGSIGTAILITLMTQQTTAHVADYGNMLTTSNPILVDKVHGMGQSLAALAGSAQAGDAMSTQLLFGQISKLS
AINGINDAFLIATILAGIAWVLSFFLPSGNKPNRKAGN

Specific function: Translocase That Confers Resistance To Substances Of High Hydrophobicity. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=505, Percent_Identity=28.1188118811881, Blast_Score=206, Evalue=3e-54,
Organism=Escherichia coli, GI1788710, Length=504, Percent_Identity=26.1904761904762, Blast_Score=168, Evalue=8e-43,
Organism=Escherichia coli, GI87081983, Length=407, Percent_Identity=25.0614250614251, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1790195, Length=338, Percent_Identity=26.3313609467456, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI1788392, Length=422, Percent_Identity=24.8815165876777, Blast_Score=97, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6322958, Length=374, Percent_Identity=26.7379679144385, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6325455, Length=285, Percent_Identity=23.859649122807, Blast_Score=68, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6323735, Length=318, Percent_Identity=24.5283018867925, Blast_Score=68, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 64886; Mature: 64755

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
5.8 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
5.7 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSIVIAGYALFCIIVFFLVNRLLRKKKTKMGEEQVPAVSKIEVSKVETEEKDIEHKQEP
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHHEEHHHHHCHHHHCCCCCCC
EISNVVELETGKQEEVKQEKEVPKKQMSMPVENVNVKAVVAVLILGMFVSILNQTIINVA
CHHCEEEECCCCHHHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LPPLMNEFNVSTSTAQWLITGFMLVNGILVPISAFLVSRFTYRKLFVAAMLFFTVGSIIC
CCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
ATSGNFTMMMTGRVIQAVGAGILMPVGMNIFMTLFPPHKRGAAMGLLGVAMILAPAIGPT
ECCCCEEEEEHHHHHHHHCCCHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCC
VTGWVIENYSWNLMFYAMFIIGLIITFLSLKFFTLAQPVSNTKLDVFGVVSSSIGLGSLL
EEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHCCCCHHHHH
YGFSEAGNNSWTSAEVVISLIIGVIGLALFIWRELTTDNKMLDLQVFKYPVFTFTLVINA
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHH
IVTMALFGGMLLLPVYLQNIRGFTPIESGLLLLPGSLIMGIMGPVAGKLFDKYGIRPLAI
HHHHHHHHHHHHHHHHHHHCCCCCCHHCCCEECCHHHHHHHHHHHHHHHHHHHCCCHHHH
IGLAITTYATYEFTKLSMDTPYSVIMTDYIIRSIGMSFIMMPIMTAGMNALPMKLISHGT
HHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCC
ATQNTSRQVAGSIGTAILITLMTQQTTAHVADYGNMLTTSNPILVDKVHGMGQSLAALAG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCEEEECHHCCCHHHHHHHC
SAQAGDAMSTQLLFGQISKLSAINGINDAFLIATILAGIAWVLSFFLPSGNKPNRKAGN
CCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SSIVIAGYALFCIIVFFLVNRLLRKKKTKMGEEQVPAVSKIEVSKVETEEKDIEHKQEP
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHHEEHHHHHCHHHHCCCCCCC
EISNVVELETGKQEEVKQEKEVPKKQMSMPVENVNVKAVVAVLILGMFVSILNQTIINVA
CHHCEEEECCCCHHHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LPPLMNEFNVSTSTAQWLITGFMLVNGILVPISAFLVSRFTYRKLFVAAMLFFTVGSIIC
CCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
ATSGNFTMMMTGRVIQAVGAGILMPVGMNIFMTLFPPHKRGAAMGLLGVAMILAPAIGPT
ECCCCEEEEEHHHHHHHHCCCHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCC
VTGWVIENYSWNLMFYAMFIIGLIITFLSLKFFTLAQPVSNTKLDVFGVVSSSIGLGSLL
EEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHCCCCHHHHH
YGFSEAGNNSWTSAEVVISLIIGVIGLALFIWRELTTDNKMLDLQVFKYPVFTFTLVINA
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHH
IVTMALFGGMLLLPVYLQNIRGFTPIESGLLLLPGSLIMGIMGPVAGKLFDKYGIRPLAI
HHHHHHHHHHHHHHHHHHHCCCCCCHHCCCEECCHHHHHHHHHHHHHHHHHHHCCCHHHH
IGLAITTYATYEFTKLSMDTPYSVIMTDYIIRSIGMSFIMMPIMTAGMNALPMKLISHGT
HHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCC
ATQNTSRQVAGSIGTAILITLMTQQTTAHVADYGNMLTTSNPILVDKVHGMGQSLAALAG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCEEEECHHCCCHHHHHHHC
SAQAGDAMSTQLLFGQISKLSAINGINDAFLIATILAGIAWVLSFFLPSGNKPNRKAGN
CCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 8969498 [H]