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The map label for this gene is spoU

Identifier: 49478690

GI number: 49478690

Start: 4362531

End: 4363295

Strand: Reverse

Name: spoU

Synonym: BT9727_4295

Alternate gene names: 49478690

Gene position: 4363295-4362531 (Counterclockwise)

Preceding gene: 49480683

Following gene: 49481330

Centisome position: 83.31

GC content: 38.43

Gene sequence:

>765_bases
ATGAAAAACATTGATTCAGTACAAAATCCGCGTGTGAAGCAGTGGAAAAAGCTGCAAACGAAAAAAGAACGCGATAAAAA
AGGTTTATTTTTTGTAGAAGGATTCCATTTAGTGGAGGAAGCTTTAAAAGCAGGAGTTGTAACAGAACTTATCGTTTCAG
ATCAGACAGACCTTCCGAAAGATTGGACAGTTAATGATGTAGAAATGTATATCGTACCGGAAGTAATCGTAAAAGTACTT
CGTGAAACAGAAACGACGCAAGGTGTATTTGCTGTTTGTGAGAAACACGAGAGTGAAGTAGCTCTTACTGATGGGAAGTT
TCTTTTATTAGATGGCTTGCAAGACCCAGGTAACTTAGGGACAATTATTCGCACAGCTGATGCAGCTGGTATTCATGCCG
TTGTGCTTGGAGAAGGTTGTGTTGATGCATATAATAGTAAAGTACTCCGCTCTACACAAGGCTCTATTTTCCACTTACCA
ATTGTAAAAGGAAACTTAGAAGAATGGGTAGACAAACTAAAAGAAAATAACGTTCCTGTATATGGAACAGCTCTTGAAAA
TGGAGTTCCTTACGGTGAAGTAACACCAGCAGGAAGTTTTGCATTAATTGTAGGAAATGAAGGAAACGGCGTACGCCAAG
AAATTCTTGCAAAAACAGATCAAAACTTGTATATTCCGATTTATGGCGGGGCAGAATCATTAAATGTTGCAGTTGCAGCA
GGGATTTTAACGTATTATTTACAAAGCCCAGTTGCGAACAAATAA

Upstream 100 bases:

>100_bases
TTCAGAATTGAAACTGACGTTAGTTTTTGTTCTTTTTTATGTAAATATGTATGAAAAATAGGCTATAATGATAAGACAAG
AAGAGAGGGAAATACATATT

Downstream 100 bases:

>100_bases
AAAACTTCTTATAATAAGTTGAAGTATATTATATTAAAAGTTAAATAACGAAAAACGTTGATAGAGAAGAGTAGCTTACA
AAATCGCCATATAGGGAGAG

Product: 23S rRNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MKNIDSVQNPRVKQWKKLQTKKERDKKGLFFVEGFHLVEEALKAGVVTELIVSDQTDLPKDWTVNDVEMYIVPEVIVKVL
RETETTQGVFAVCEKHESEVALTDGKFLLLDGLQDPGNLGTIIRTADAAGIHAVVLGEGCVDAYNSKVLRSTQGSIFHLP
IVKGNLEEWVDKLKENNVPVYGTALENGVPYGEVTPAGSFALIVGNEGNGVRQEILAKTDQNLYIPIYGGAESLNVAVAA
GILTYYLQSPVANK

Sequences:

>Translated_254_residues
MKNIDSVQNPRVKQWKKLQTKKERDKKGLFFVEGFHLVEEALKAGVVTELIVSDQTDLPKDWTVNDVEMYIVPEVIVKVL
RETETTQGVFAVCEKHESEVALTDGKFLLLDGLQDPGNLGTIIRTADAAGIHAVVLGEGCVDAYNSKVLRSTQGSIFHLP
IVKGNLEEWVDKLKENNVPVYGTALENGVPYGEVTPAGSFALIVGNEGNGVRQEILAKTDQNLYIPIYGGAESLNVAVAA
GILTYYLQSPVANK
>Mature_254_residues
MKNIDSVQNPRVKQWKKLQTKKERDKKGLFFVEGFHLVEEALKAGVVTELIVSDQTDLPKDWTVNDVEMYIVPEVIVKVL
RETETTQGVFAVCEKHESEVALTDGKFLLLDGLQDPGNLGTIIRTADAAGIHAVVLGEGCVDAYNSKVLRSTQGSIFHLP
IVKGNLEEWVDKLKENNVPVYGTALENGVPYGEVTPAGSFALIVGNEGNGVRQEILAKTDQNLYIPIYGGAESLNVAVAA
GILTYYLQSPVANK

Specific function: Unknown

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Homo sapiens, GI8922534, Length=291, Percent_Identity=28.5223367697594, Blast_Score=96, Evalue=4e-20,
Organism=Escherichia coli, GI1790623, Length=148, Percent_Identity=36.4864864864865, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1790083, Length=149, Percent_Identity=29.5302013422819, Blast_Score=64, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24666840, Length=278, Percent_Identity=27.3381294964029, Blast_Score=89, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27738; Mature: 27738

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNIDSVQNPRVKQWKKLQTKKERDKKGLFFVEGFHLVEEALKAGVVTELIVSDQTDLPK
CCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHCCHHHHHHHCCCCCCCC
DWTVNDVEMYIVPEVIVKVLRETETTQGVFAVCEKHESEVALTDGKFLLLDGLQDPGNLG
CCCCCCEEEEEEHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEECCCEEEEECCCCCCCCC
TIIRTADAAGIHAVVLGEGCVDAYNSKVLRSTQGSIFHLPIVKGNLEEWVDKLKENNVPV
EEEEECCCCCEEEEEECCCHHHHHCCHHHHCCCCCEEEEEEEECCHHHHHHHHHHCCCCE
YGTALENGVPYGEVTPAGSFALIVGNEGNGVRQEILAKTDQNLYIPIYGGAESLNVAVAA
EEEHHHCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCEEEEEECCCCHHHHHHHH
GILTYYLQSPVANK
HHHHHHHHCCCCCC
>Mature Secondary Structure
MKNIDSVQNPRVKQWKKLQTKKERDKKGLFFVEGFHLVEEALKAGVVTELIVSDQTDLPK
CCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHCCHHHHHHHCCCCCCCC
DWTVNDVEMYIVPEVIVKVLRETETTQGVFAVCEKHESEVALTDGKFLLLDGLQDPGNLG
CCCCCCEEEEEEHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEECCCEEEEECCCCCCCCC
TIIRTADAAGIHAVVLGEGCVDAYNSKVLRSTQGSIFHLPIVKGNLEEWVDKLKENNVPV
EEEEECCCCCEEEEEECCCHHHHHCCHHHHCCCCCEEEEEEEECCHHHHHHHHHHCCCCE
YGTALENGVPYGEVTPAGSFALIVGNEGNGVRQEILAKTDQNLYIPIYGGAESLNVAVAA
EEEHHHCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCEEEEEECCCCHHHHHHHH
GILTYYLQSPVANK
HHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]