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The map label for this gene is glcD [H]

Identifier: 49478981

GI number: 49478981

Start: 3345188

End: 3346579

Strand: Reverse

Name: glcD [H]

Synonym: BT9727_3278

Alternate gene names: 49478981

Gene position: 3346579-3345188 (Counterclockwise)

Preceding gene: 49479484

Following gene: 49477708

Centisome position: 63.89

GC content: 38.86

Gene sequence:

>1392_bases
ATGGAAATAACAGTAGAGCGTTTAGTAAATGAATTAAAGGGTGTACTTCCCGAAGATCGAGTAGTAATCAATACGACAGT
AAGAGAGTTACATAGTAAAGATGAATCTTACCATGCTAGTAGTTTACCTGATGTAGTAGTTTTTCCGAAAACGACAGAAG
AAGTTAGCACGATAATGAAAGTAGCGAGTGAGCACGGGACACCCGTCGTTCCGTTTGGAGTAGGGTCCAGTTTAGAGGGG
CACGTAATTCCTTATGAAAAAGGGATTACAGTTGATTTTTCTTTAATGAACAAAATTCTTGAAATAAGAGAGAAAGATTT
TCTTGTGAGAGTACAGCCGGGAGTGACGCGTTCTCAGCTTAATAAAGAGTTAAAGAAATACGGTTTATTTTTTAGTGTAG
ATCCAGGGGCAGATGCAACGTTAGGTGGAATGGCCGCTACAAATGCGAGTGGAACGACAGCCGTGAAATATGGCGTTATG
CGTGATCAAGTTCGTGATTTAGAAGTCGTTCTTGCAGATGGAGAAGTAATACATACTGGGAATTTAGCTGCGAAGTCGTC
ATCAGGTTACCATTTAAATGGTGTTTTCGTAGGATCAGAAGGGACACTTGGATGTTTTACGGAGTTAACATTAAAAGTAT
ACGGCATACCAGAACATGTTATGGCCGCAAGAGCGTCCTTTCCAACTATAAATGATGCAGTAGAAGCTGTTATTAACATA
TTGCAAGCGGGTATTCCGATTGCGAGAATTGAACTCGTTGATGAACTATCTATGAAACAAGTCAATCATTATAGTGAAAC
AAGTTACAGAGAAGAACCTACATTGTTTTTAGAGTTTCACGGTAATGAAGCAGGGTTAAAGCAAGATGTTGAATTTACGA
AGGAAATTGTTTTTGATCATAAATGTAAAGAAGTTGCTTTTGAAACGGAAACTGCAGCGAGAAATAAATTATGGGATGCA
CGGCATAATCTAGCATATGCTTACGTTCATAGCTATCCTGGTAAAAAGCTTATGAGTACAGACGTATGTGTACCTATTTC
AGAATTAGCTGGAGCTATCCAACAAGCGAAAGAGACGTTAGAAAAGAATGGTCTTGTTGGTGGTATTCTTGGTCATGTAG
GAGACGGGAATTTCCACGTGCTCTTAATGGTTGATCCAAACGATAAAGAAGAAGTGGAAAAAGCAGATAAAATAAATGAA
AGTATCGTTCTATATGCTCTTAAACGAGGAGGAACATGTACAGGGGAACATGGCGTTGGTATTGGAAAACGGAAGTATCA
GGAAGAAGAACATGGGGCGGCATTACTCGTAATGGAAAAAATAAAGAAAGCTCTTGATCCGCAAAATATTTTGAATCCGA
ATAAGGTGTTTCAATTAAAGGATAGGAGCTGA

Upstream 100 bases:

>100_bases
GTATATGTAAAACTTGAATTATTTGATTTTTCGGATTATTATGTATAAAACACTTAACATATTTTGTAATTCAAATTTAT
AGAGAGTAGGAGAGTGTACG

Downstream 100 bases:

>100_bases
TCGGGTGAAAGTACTAGAAGCGATAAGTAATATAGCTGTAAAGTATTTTGCATTTTGGGTTATTTGTATTGCAACTATTG
CGTATTTTATTCCCGCTCCA

Product: glycolate oxidase, GlcD subunit

Products: pyruvate; ferrocytochrome c

Alternate protein names: NA

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MEITVERLVNELKGVLPEDRVVINTTVRELHSKDESYHASSLPDVVVFPKTTEEVSTIMKVASEHGTPVVPFGVGSSLEG
HVIPYEKGITVDFSLMNKILEIREKDFLVRVQPGVTRSQLNKELKKYGLFFSVDPGADATLGGMAATNASGTTAVKYGVM
RDQVRDLEVVLADGEVIHTGNLAAKSSSGYHLNGVFVGSEGTLGCFTELTLKVYGIPEHVMAARASFPTINDAVEAVINI
LQAGIPIARIELVDELSMKQVNHYSETSYREEPTLFLEFHGNEAGLKQDVEFTKEIVFDHKCKEVAFETETAARNKLWDA
RHNLAYAYVHSYPGKKLMSTDVCVPISELAGAIQQAKETLEKNGLVGGILGHVGDGNFHVLLMVDPNDKEEVEKADKINE
SIVLYALKRGGTCTGEHGVGIGKRKYQEEEHGAALLVMEKIKKALDPQNILNPNKVFQLKDRS

Sequences:

>Translated_463_residues
MEITVERLVNELKGVLPEDRVVINTTVRELHSKDESYHASSLPDVVVFPKTTEEVSTIMKVASEHGTPVVPFGVGSSLEG
HVIPYEKGITVDFSLMNKILEIREKDFLVRVQPGVTRSQLNKELKKYGLFFSVDPGADATLGGMAATNASGTTAVKYGVM
RDQVRDLEVVLADGEVIHTGNLAAKSSSGYHLNGVFVGSEGTLGCFTELTLKVYGIPEHVMAARASFPTINDAVEAVINI
LQAGIPIARIELVDELSMKQVNHYSETSYREEPTLFLEFHGNEAGLKQDVEFTKEIVFDHKCKEVAFETETAARNKLWDA
RHNLAYAYVHSYPGKKLMSTDVCVPISELAGAIQQAKETLEKNGLVGGILGHVGDGNFHVLLMVDPNDKEEVEKADKINE
SIVLYALKRGGTCTGEHGVGIGKRKYQEEEHGAALLVMEKIKKALDPQNILNPNKVFQLKDRS
>Mature_463_residues
MEITVERLVNELKGVLPEDRVVINTTVRELHSKDESYHASSLPDVVVFPKTTEEVSTIMKVASEHGTPVVPFGVGSSLEG
HVIPYEKGITVDFSLMNKILEIREKDFLVRVQPGVTRSQLNKELKKYGLFFSVDPGADATLGGMAATNASGTTAVKYGVM
RDQVRDLEVVLADGEVIHTGNLAAKSSSGYHLNGVFVGSEGTLGCFTELTLKVYGIPEHVMAARASFPTINDAVEAVINI
LQAGIPIARIELVDELSMKQVNHYSETSYREEPTLFLEFHGNEAGLKQDVEFTKEIVFDHKCKEVAFETETAARNKLWDA
RHNLAYAYVHSYPGKKLMSTDVCVPISELAGAIQQAKETLEKNGLVGGILGHVGDGNFHVLLMVDPNDKEEVEKADKINE
SIVLYALKRGGTCTGEHGVGIGKRKYQEEEHGAALLVMEKIKKALDPQNILNPNKVFQLKDRS

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=454, Percent_Identity=47.7973568281938, Blast_Score=444, Evalue=1e-124,
Organism=Homo sapiens, GI37595754, Length=477, Percent_Identity=45.2830188679245, Blast_Score=433, Evalue=1e-121,
Organism=Homo sapiens, GI119964728, Length=431, Percent_Identity=26.6821345707657, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI4501993, Length=219, Percent_Identity=30.1369863013699, Blast_Score=106, Evalue=4e-23,
Organism=Escherichia coli, GI1789351, Length=465, Percent_Identity=30.1075268817204, Blast_Score=200, Evalue=2e-52,
Organism=Escherichia coli, GI1787977, Length=494, Percent_Identity=27.1255060728745, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI48994907, Length=492, Percent_Identity=24.5934959349593, Blast_Score=112, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI71992373, Length=462, Percent_Identity=43.2900432900433, Blast_Score=385, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI71992381, Length=462, Percent_Identity=42.4242424242424, Blast_Score=372, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI71992392, Length=361, Percent_Identity=47.3684210526316, Blast_Score=337, Evalue=7e-93,
Organism=Caenorhabditis elegans, GI17534361, Length=453, Percent_Identity=26.7108167770419, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17556096, Length=452, Percent_Identity=25.8849557522124, Blast_Score=123, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6320027, Length=474, Percent_Identity=37.9746835443038, Blast_Score=315, Evalue=1e-86,
Organism=Saccharomyces cerevisiae, GI6320764, Length=468, Percent_Identity=24.5726495726496, Blast_Score=132, Evalue=9e-32,
Organism=Saccharomyces cerevisiae, GI6320023, Length=473, Percent_Identity=25.1585623678647, Blast_Score=127, Evalue=3e-30,
Organism=Drosophila melanogaster, GI18921117, Length=431, Percent_Identity=26.2180974477958, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24639277, Length=431, Percent_Identity=26.2180974477958, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24639275, Length=431, Percent_Identity=26.2180974477958, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24653753, Length=219, Percent_Identity=31.0502283105023, Blast_Score=103, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.2.4

Molecular weight: Translated: 50816; Mature: 50816

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEITVERLVNELKGVLPEDRVVINTTVRELHSKDESYHASSLPDVVVFPKTTEEVSTIMK
CCEEHHHHHHHHHCCCCCCCEEEEHHHHHHHCCCCCCCCCCCCCEEEECCCHHHHHHHHH
VASEHGTPVVPFGVGSSLEGHVIPYEKGITVDFSLMNKILEIREKDFLVRVQPGVTRSQL
HHHHCCCEEEEECCCCCCCCCEEECCCCCEEEHHHHHHHHHHHCCCEEEEECCCCCHHHH
NKELKKYGLFFSVDPGADATLGGMAATNASGTTAVKYGVMRDQVRDLEVVLADGEVIHTG
HHHHHHHCCEEEECCCCCCCCCCEEECCCCCCHHEEHHHHHHHHCCEEEEEECCCEEECC
NLAAKSSSGYHLNGVFVGSEGTLGCFTELTLKVYGIPEHVMAARASFPTINDAVEAVINI
CCEECCCCCEEEEEEEECCCCCEEEEEEEEEEEECCCHHHHHHHCCCCCHHHHHHHHHHH
LQAGIPIARIELVDELSMKQVNHYSETSYREEPTLFLEFHGNEAGLKQDVEFTKEIVFDH
HHCCCCEEHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCC
KCKEVAFETETAARNKLWDARHNLAYAYVHSYPGKKLMSTDVCVPISELAGAIQQAKETL
HHHHHHHCCHHHHHHHHHHHHHCEEEEEEECCCCCCCCCCCCCEEHHHHHHHHHHHHHHH
EKNGLVGGILGHVGDGNFHVLLMVDPNDKEEVEKADKINESIVLYALKRGGTCTGEHGVG
HHCCCEEEEHHCCCCCCEEEEEEECCCCHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCC
IGKRKYQEEEHGAALLVMEKIKKALDPQNILNPNKVFQLKDRS
CCHHHCCHHHCCCCHHHHHHHHHHCCHHHCCCCCCEEEECCCC
>Mature Secondary Structure
MEITVERLVNELKGVLPEDRVVINTTVRELHSKDESYHASSLPDVVVFPKTTEEVSTIMK
CCEEHHHHHHHHHCCCCCCCEEEEHHHHHHHCCCCCCCCCCCCCEEEECCCHHHHHHHHH
VASEHGTPVVPFGVGSSLEGHVIPYEKGITVDFSLMNKILEIREKDFLVRVQPGVTRSQL
HHHHCCCEEEEECCCCCCCCCEEECCCCCEEEHHHHHHHHHHHCCCEEEEECCCCCHHHH
NKELKKYGLFFSVDPGADATLGGMAATNASGTTAVKYGVMRDQVRDLEVVLADGEVIHTG
HHHHHHHCCEEEECCCCCCCCCCEEECCCCCCHHEEHHHHHHHHCCEEEEEECCCEEECC
NLAAKSSSGYHLNGVFVGSEGTLGCFTELTLKVYGIPEHVMAARASFPTINDAVEAVINI
CCEECCCCCEEEEEEEECCCCCEEEEEEEEEEEECCCHHHHHHHCCCCCHHHHHHHHHHH
LQAGIPIARIELVDELSMKQVNHYSETSYREEPTLFLEFHGNEAGLKQDVEFTKEIVFDH
HHCCCCEEHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCC
KCKEVAFETETAARNKLWDARHNLAYAYVHSYPGKKLMSTDVCVPISELAGAIQQAKETL
HHHHHHHCCHHHHHHHHHHHHHCEEEEEEECCCCCCCCCCCCCEEHHHHHHHHHHHHHHH
EKNGLVGGILGHVGDGNFHVLLMVDPNDKEEVEKADKINESIVLYALKRGGTCTGEHGVG
HHCCCEEEEHHCCCCCCEEEEEEECCCCHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCC
IGKRKYQEEEHGAALLVMEKIKKALDPQNILNPNKVFQLKDRS
CCHHHCCHHHCCCCHHHHHHHHHHCCHHHCCCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (R)-lactate; ferricytochrome c

Specific reaction: (R)-lactate + 2 ferricytochrome c = pyruvate + 2 ferrocytochrome c

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]