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The map label for this gene is wcaL [C]

Identifier: 49478938

GI number: 49478938

Start: 5003456

End: 5004538

Strand: Reverse

Name: wcaL [C]

Synonym: BT9727_4950

Alternate gene names: 49478938

Gene position: 5004538-5003456 (Counterclockwise)

Preceding gene: 49478940

Following gene: 49478937

Centisome position: 95.55

GC content: 30.01

Gene sequence:

>1083_bases
ATGAAAGATGAAATTATTCTGGTTGGACCAGGAACAGATTCTATGGGGGGCGTCGCTACTCATATAAAGACTTTACAAAC
CTGTTTTGAAGAAAATGGGAAAACTATTCTGATACATGATATTAGTGGAGGAAAAGAGAAAAATTTTTTGTTAAGAAAGA
TTAAAGAGTTCAGTAAAATATGGGGATTGAGGAAGGTTACGAAAAATAAGAAGGATAGTGTGTTACATTTAAATCCTTCG
ATTTATTATGGATCTTTAATGAAATTATGGATTATATTGTCGATCATTAACCATGAAAATATTATTGTCCAATATCATGG
GGGCCATTTTTCAGACTTAAATTTATTTAAAAATAAATTTATTAGATTCCTATTTAAAAAGATTAATACTAAGCCAAAAC
AAATTCTTTTTTTGAGTAAAGATCAAAAAATGGAGTTTGAATATGTTTACCCAAATGTAAAAGCTAAAGTAAGTTTGGTG
CCTAATTTTATTGATGTTAGAGGGATTGAGAAAAAAAATGTTTTTACTGATGGAAAAGTGAAATTTCTTTTTTTATCCAG
GTTAATTCAAGAAAAAGGTGTATTCGAAGTCTTGGAAGCGGCTAAATTACTTCAAGATAAAGAAGTTGAATTTACAATTA
TAGGTTCAGGGCCTATAGAGGGACAAGTCATAGAATATATAGAGGAAAATAATCTCCATGGGATAGTTAAATTTGTAGGC
GCAAAATTTGATGGAGAGAAAGAAGAATATTTGGGTTCATCTCATGTTTTTTTACTTCCAAGTAGCTGGAGAGAAGGAAT
ACCTTATTCGGTGTTAGAGGCAATGAAATTCAGCTTAGCAATTCTTTGTACGCCGGTAGGTGGATTAAAAGAGATTATTA
TTGATGGGGATAATGGCCTATTTATACAAAAGGATTCAAATAATATATACCAAAAAGTGCTGTATTTGTTAGAGGATACA
GATAAGATTAATCAATTTGGTGGAAATGCATTTGATTATGCGTCTAATTATTTATCTTATGAAAAAGCGTTTAATACATT
TATGAATATATATAGTGAAACACAAACGATTAGGAGGAAATAA

Upstream 100 bases:

>100_bases
TGGTCATCAGTATATATGGTAGTTTGGCGTGGGTCTTTAGATTAATAAATACTTAAATTTAAATGAAAATATTTAGATTT
AACCTAAGGAGATAGTTTTT

Downstream 100 bases:

>100_bases
TTATGAAGAAAATATGTGTTGTGGGCTTAGGATATATAGGATTACCAACTTGTGCAATGTTTGCCAAGGCTGGATTTAAG
GTCACAGGAGTAGATGTTAA

Product: glycosyl transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MKDEIILVGPGTDSMGGVATHIKTLQTCFEENGKTILIHDISGGKEKNFLLRKIKEFSKIWGLRKVTKNKKDSVLHLNPS
IYYGSLMKLWIILSIINHENIIVQYHGGHFSDLNLFKNKFIRFLFKKINTKPKQILFLSKDQKMEFEYVYPNVKAKVSLV
PNFIDVRGIEKKNVFTDGKVKFLFLSRLIQEKGVFEVLEAAKLLQDKEVEFTIIGSGPIEGQVIEYIEENNLHGIVKFVG
AKFDGEKEEYLGSSHVFLLPSSWREGIPYSVLEAMKFSLAILCTPVGGLKEIIIDGDNGLFIQKDSNNIYQKVLYLLEDT
DKINQFGGNAFDYASNYLSYEKAFNTFMNIYSETQTIRRK

Sequences:

>Translated_360_residues
MKDEIILVGPGTDSMGGVATHIKTLQTCFEENGKTILIHDISGGKEKNFLLRKIKEFSKIWGLRKVTKNKKDSVLHLNPS
IYYGSLMKLWIILSIINHENIIVQYHGGHFSDLNLFKNKFIRFLFKKINTKPKQILFLSKDQKMEFEYVYPNVKAKVSLV
PNFIDVRGIEKKNVFTDGKVKFLFLSRLIQEKGVFEVLEAAKLLQDKEVEFTIIGSGPIEGQVIEYIEENNLHGIVKFVG
AKFDGEKEEYLGSSHVFLLPSSWREGIPYSVLEAMKFSLAILCTPVGGLKEIIIDGDNGLFIQKDSNNIYQKVLYLLEDT
DKINQFGGNAFDYASNYLSYEKAFNTFMNIYSETQTIRRK
>Mature_360_residues
MKDEIILVGPGTDSMGGVATHIKTLQTCFEENGKTILIHDISGGKEKNFLLRKIKEFSKIWGLRKVTKNKKDSVLHLNPS
IYYGSLMKLWIILSIINHENIIVQYHGGHFSDLNLFKNKFIRFLFKKINTKPKQILFLSKDQKMEFEYVYPNVKAKVSLV
PNFIDVRGIEKKNVFTDGKVKFLFLSRLIQEKGVFEVLEAAKLLQDKEVEFTIIGSGPIEGQVIEYIEENNLHGIVKFVG
AKFDGEKEEYLGSSHVFLLPSSWREGIPYSVLEAMKFSLAILCTPVGGLKEIIIDGDNGLFIQKDSNNIYQKVLYLLEDT
DKINQFGGNAFDYASNYLSYEKAFNTFMNIYSETQTIRRK

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0438

COG function: function code M; Glycosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1788357, Length=133, Percent_Identity=29.3233082706767, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296 [H]

Pfam domain/function: PF00534 Glycos_transf_1 [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 41210; Mature: 41210

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDEIILVGPGTDSMGGVATHIKTLQTCFEENGKTILIHDISGGKEKNFLLRKIKEFSKI
CCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHH
WGLRKVTKNKKDSVLHLNPSIYYGSLMKLWIILSIINHENIIVQYHGGHFSDLNLFKNKF
HHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHH
IRFLFKKINTKPKQILFLSKDQKMEFEYVYPNVKAKVSLVPNFIDVRGIEKKNVFTDGKV
HHHHHHHHCCCCCEEEEEECCCCEEEEEECCCCEEEEEECCCCHHCCCCCCCCCCCCCCC
KFLFLSRLIQEKGVFEVLEAAKLLQDKEVEFTIIGSGPIEGQVIEYIEENNLHGIVKFVG
HHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHCCCCCEEEHHHC
AKFDGEKEEYLGSSHVFLLPSSWREGIPYSVLEAMKFSLAILCTPVGGLKEIIIDGDNGL
CCCCCCHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHEEEEEECCCCCEEEEEECCCCE
FIQKDSNNIYQKVLYLLEDTDKINQFGGNAFDYASNYLSYEKAFNTFMNIYSETQTIRRK
EEEECCCHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKDEIILVGPGTDSMGGVATHIKTLQTCFEENGKTILIHDISGGKEKNFLLRKIKEFSKI
CCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHH
WGLRKVTKNKKDSVLHLNPSIYYGSLMKLWIILSIINHENIIVQYHGGHFSDLNLFKNKF
HHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHH
IRFLFKKINTKPKQILFLSKDQKMEFEYVYPNVKAKVSLVPNFIDVRGIEKKNVFTDGKV
HHHHHHHHCCCCCEEEEEECCCCEEEEEECCCCEEEEEECCCCHHCCCCCCCCCCCCCCC
KFLFLSRLIQEKGVFEVLEAAKLLQDKEVEFTIIGSGPIEGQVIEYIEENNLHGIVKFVG
HHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHCCCCCEEEHHHC
AKFDGEKEEYLGSSHVFLLPSSWREGIPYSVLEAMKFSLAILCTPVGGLKEIIIDGDNGL
CCCCCCHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHEEEEEECCCCCEEEEEECCCCE
FIQKDSNNIYQKVLYLLEDTDKINQFGGNAFDYASNYLSYEKAFNTFMNIYSETQTIRRK
EEEECCCHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]