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The map label for this gene is hemB

Identifier: 49478644

GI number: 49478644

Start: 4259601

End: 4260590

Strand: Reverse

Name: hemB

Synonym: BT9727_4195

Alternate gene names: 49478644

Gene position: 4260590-4259601 (Counterclockwise)

Preceding gene: 49478645

Following gene: 49478643

Centisome position: 81.34

GC content: 38.89

Gene sequence:

>990_bases
ATGAACTCTTTACAATTTAATCGTCATCGTCGCTTAAGACAAAGCGGGGGCATGCGTGCGCTTGTGCGTGAAACGTTTTT
ACATACGGAAGATTTTATTTACCCTATTTTTGTATTAGAAGGTGAAAATGTTCGTAATGAAGTACCTTCTATGCCAGGCG
TATATCAAATGTCTTTAGATTTATTGCAAGCTGAAATGCAAGAAGTTGTTGATTTAGGTATTCGTTCTGTTATTGTATTT
GGTTTACCTGCTGAAAAAGATGAAGTTGGATCAGCAGCATATTGTGATCATGGAATTGTGCAACGCGCGATTCAGCAAAT
TAAAGGTGAATTCCCCGATCTAGTAGTAGTTGCGGATACATGTTTATGTCAATTTACAAGCCATGGTCATTGCGGTGTAA
TTGAAGATGGTATTATTTTAAATGACGAGTCTCTTGCAGTTCTTGCAAAAACCGCTGTAAGCCAAGCGAAAGCAGGAGCG
GACATTATTGCGCCATCAAACATGATGGACGGATTCGTAACAGCAATTCGCCACGCATTAGATGAAAATGGTTTTGGACA
TGTACCAGTTATGTCGTATGCTGTGAAATATTCATCAGCATTTTATGGACCATTCCGTGATGCGGCACACGGTGCACCGC
AATTTGGTGATCGTAAAACATATCAAATGGACCCAGCGAACCGCATGGAAGCATTCCGTGAAGCAGAATCAGATGTAATG
GAAGGGGCAGATTTCTTAATTGTAAAACCAGCTCTTTCTTACTTAGATATCGTTCGTGATGTGAAAAATAACTTTAATTT
ACCAGTCGTTGCTTATAACGTAAGCGGTGAATATTCAATGATTAAAGCGGCAGCGCAAAATGGTTGGATTAATGAAAAAG
AAGTTGTACTTGAAAAATTAATTAGTATGAAACGTGCAGGAGCAGATTTAATTATTACGTATCATGCAAAAGATGCAGCA
AGATGGTTACAAGAAGGAGGCGCTAAATAA

Upstream 100 bases:

>100_bases
TTTATTTTCCGCATGTTATTATGCCGAAAGAGTACACTGTAGAAGCATTACTACAATGCGTTTGTGAATCTATAAAATAA
AAGAATGGGGATAGAAATCT

Downstream 100 bases:

>100_bases
TGAGAAAATTTGATAAGTCGATTGCAGCGTTTGAAGAGGCTCAAGATTTAATGCCTGGTGGCGTAAATAGCCCGGTTCGT
GCATTTAAGTCTGTCGGTAT

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MNSLQFNRHRRLRQSGGMRALVRETFLHTEDFIYPIFVLEGENVRNEVPSMPGVYQMSLDLLQAEMQEVVDLGIRSVIVF
GLPAEKDEVGSAAYCDHGIVQRAIQQIKGEFPDLVVVADTCLCQFTSHGHCGVIEDGIILNDESLAVLAKTAVSQAKAGA
DIIAPSNMMDGFVTAIRHALDENGFGHVPVMSYAVKYSSAFYGPFRDAAHGAPQFGDRKTYQMDPANRMEAFREAESDVM
EGADFLIVKPALSYLDIVRDVKNNFNLPVVAYNVSGEYSMIKAAAQNGWINEKEVVLEKLISMKRAGADLIITYHAKDAA
RWLQEGGAK

Sequences:

>Translated_329_residues
MNSLQFNRHRRLRQSGGMRALVRETFLHTEDFIYPIFVLEGENVRNEVPSMPGVYQMSLDLLQAEMQEVVDLGIRSVIVF
GLPAEKDEVGSAAYCDHGIVQRAIQQIKGEFPDLVVVADTCLCQFTSHGHCGVIEDGIILNDESLAVLAKTAVSQAKAGA
DIIAPSNMMDGFVTAIRHALDENGFGHVPVMSYAVKYSSAFYGPFRDAAHGAPQFGDRKTYQMDPANRMEAFREAESDVM
EGADFLIVKPALSYLDIVRDVKNNFNLPVVAYNVSGEYSMIKAAAQNGWINEKEVVLEKLISMKRAGADLIITYHAKDAA
RWLQEGGAK
>Mature_329_residues
MNSLQFNRHRRLRQSGGMRALVRETFLHTEDFIYPIFVLEGENVRNEVPSMPGVYQMSLDLLQAEMQEVVDLGIRSVIVF
GLPAEKDEVGSAAYCDHGIVQRAIQQIKGEFPDLVVVADTCLCQFTSHGHCGVIEDGIILNDESLAVLAKTAVSQAKAGA
DIIAPSNMMDGFVTAIRHALDENGFGHVPVMSYAVKYSSAFYGPFRDAAHGAPQFGDRKTYQMDPANRMEAFREAESDVM
EGADFLIVKPALSYLDIVRDVKNNFNLPVVAYNVSGEYSMIKAAAQNGWINEKEVVLEKLISMKRAGADLIITYHAKDAA
RWLQEGGAK

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=322, Percent_Identity=45.0310559006211, Blast_Score=279, Evalue=3e-75,
Organism=Escherichia coli, GI87081728, Length=315, Percent_Identity=50.7936507936508, Blast_Score=296, Evalue=1e-81,
Organism=Saccharomyces cerevisiae, GI6321398, Length=323, Percent_Identity=43.6532507739938, Blast_Score=253, Evalue=2e-68,
Organism=Drosophila melanogaster, GI21358291, Length=309, Percent_Identity=40.1294498381877, Blast_Score=243, Evalue=2e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 36324; Mature: 36324

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSLQFNRHRRLRQSGGMRALVRETFLHTEDFIYPIFVLEGENVRNEVPSMPGVYQMSLD
CCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEEECCCHHHHCCCCCCHHHHHHH
LLQAEMQEVVDLGIRSVIVFGLPAEKDEVGSAAYCDHGIVQRAIQQIKGEFPDLVVVADT
HHHHHHHHHHHHHHHHEEEECCCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEEHHH
CLCQFTSHGHCGVIEDGIILNDESLAVLAKTAVSQAKAGADIIAPSNMMDGFVTAIRHAL
HHHHHCCCCCCCEECCCEEECCCCHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHH
DENGFGHVPVMSYAVKYSSAFYGPFRDAAHGAPQFGDRKTYQMDPANRMEAFREAESDVM
CCCCCCCCHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHH
EGADFLIVKPALSYLDIVRDVKNNFNLPVVAYNVSGEYSMIKAAAQNGWINEKEVVLEKL
CCCCEEEEHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHH
ISMKRAGADLIITYHAKDAARWLQEGGAK
HHHHHCCCCEEEEEECHHHHHHHHHCCCC
>Mature Secondary Structure
MNSLQFNRHRRLRQSGGMRALVRETFLHTEDFIYPIFVLEGENVRNEVPSMPGVYQMSLD
CCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEEECCCHHHHCCCCCCHHHHHHH
LLQAEMQEVVDLGIRSVIVFGLPAEKDEVGSAAYCDHGIVQRAIQQIKGEFPDLVVVADT
HHHHHHHHHHHHHHHHEEEECCCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEEHHH
CLCQFTSHGHCGVIEDGIILNDESLAVLAKTAVSQAKAGADIIAPSNMMDGFVTAIRHAL
HHHHHCCCCCCCEECCCEEECCCCHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHH
DENGFGHVPVMSYAVKYSSAFYGPFRDAAHGAPQFGDRKTYQMDPANRMEAFREAESDVM
CCCCCCCCHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHH
EGADFLIVKPALSYLDIVRDVKNNFNLPVVAYNVSGEYSMIKAAAQNGWINEKEVVLEKL
CCCCEEEEHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHH
ISMKRAGADLIITYHAKDAARWLQEGGAK
HHHHHCCCCEEEEEECHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1672867; 9384377 [H]