Click here to switch to the map view.

The map label for this gene is pepT

Identifier: 49478357

GI number: 49478357

Start: 3571040

End: 3572272

Strand: Reverse

Name: pepT

Synonym: BT9727_3488

Alternate gene names: 49478357

Gene position: 3572272-3571040 (Counterclockwise)

Preceding gene: 49479430

Following gene: 49478354

Centisome position: 68.2

GC content: 36.25

Gene sequence:

>1233_bases
TTGAAACAAGAACTTATTGAAAGATTTACGAGATATGTAAAGATTGATACGCAATCAAATGAAGATAGTCATACAGTGCC
AACAACACCAGGACAAATTGAGTTTGGTAAGTTATTAGTTGAAGAGTTGAAAGAAGTTGGATTAACAGAAGTAACGATGG
ATGATAATGGATATGTAATGGCAACACTTCCAGCTAATACGGATAAGGATGTTCCTGTAATCGGCTTTTTAGCACATTTA
GATACAGCAACAGACTTTACGGGGAAAAATGTAAAACCACAAATTCATGAAAACTTTGACGGGAACGCAATTACGTTAAA
TGAAGAGCTTAATGTTGTATTAACGCCAGAACAGTTCCCAGAATTACCGTCTTATAAAGGACATACGATTATTACAACGG
ATGGTACAACACTGTTAGGAGCAGATGATAAAGCTGGTCTTACAGAAATTATGGTTGCAATGAATTATTTAATACATAAT
CCGCAAATTAAACATGGGAAAGTTAGAGTTGCGTTTACGCCGGATGAAGAAATTGGCCGTGGCCCAGCACATTTTGATGT
AGAAGCGTTTGGTGCATCATTCGCTTATACGATGGATGGAGGTCCATTAGGTGGTTTAGAATATGAAAGCTTTAATGCTG
CAGGTGCAAAGTTAACGTTTAACGGAACGAATACACATCCTGGGACAGCGAAAAATAAAATGCGTAACGCGACGAAACTA
GCTATGGAGTTTAATGGGCATTTACCAGTAGAAGAAGCACCAGAGTATACGGAAGGATACGAGGGATTCTACCATTTACT
TTCTTTAAATGGTGATGTTGAACAAAGTAAAGCATATTATATTATTCGAGATTTTGATCGTAAAAACTTTGAAGCGCGTA
AAAATACCATTGAAAATATTGTGAAACAAATGCAAGAGAAATATGGACAAGATGCAGTCGTTTTAGAAATGAATGATCAA
TACTATAACATGCTTGAAAAGATTGAACCAGTAAGAGAAATTGTTGATATTGCATATGAGGCAATGAAAAGTTTAAATAT
CGAACCAAACATTCATCCGATTCGCGGTGGAACAGATGGATCACAATTATCGTATATGGGATTACCAACGCCGAACATTT
TCACAGGTGGTGAAAACTATCACGGTAAATTTGAGTATGTTTCGGTAGATGTTATGGAAAAAGCTGTTCAAGTTATTATA
GAGATTGCAAGACGATTTGAAGAGCAAGCTTAA

Upstream 100 bases:

>100_bases
AAAATGCAAATGGAATATATACCTTTACAGTCAAAAAAGTTAAAATATTTCGTTTTAAGGCTTATAATGGAGAAAGAAAT
ATAAAAGGGAGTGAACGAGC

Downstream 100 bases:

>100_bases
AAAAGAACCAAGTAGTGCAGATGTGCTACTTGGTTTTTTTAGTATAATACTCGCCATAGGTAAAAAGTCGCATAAGACTC
CCAATTTTTCCAGTTTACCG

Product: peptidase T

Products: NA

Alternate protein names: Aminotripeptidase; Tripeptidase; Tripeptide aminopeptidase [H]

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MKQELIERFTRYVKIDTQSNEDSHTVPTTPGQIEFGKLLVEELKEVGLTEVTMDDNGYVMATLPANTDKDVPVIGFLAHL
DTATDFTGKNVKPQIHENFDGNAITLNEELNVVLTPEQFPELPSYKGHTIITTDGTTLLGADDKAGLTEIMVAMNYLIHN
PQIKHGKVRVAFTPDEEIGRGPAHFDVEAFGASFAYTMDGGPLGGLEYESFNAAGAKLTFNGTNTHPGTAKNKMRNATKL
AMEFNGHLPVEEAPEYTEGYEGFYHLLSLNGDVEQSKAYYIIRDFDRKNFEARKNTIENIVKQMQEKYGQDAVVLEMNDQ
YYNMLEKIEPVREIVDIAYEAMKSLNIEPNIHPIRGGTDGSQLSYMGLPTPNIFTGGENYHGKFEYVSVDVMEKAVQVII
EIARRFEEQA

Sequences:

>Translated_410_residues
MKQELIERFTRYVKIDTQSNEDSHTVPTTPGQIEFGKLLVEELKEVGLTEVTMDDNGYVMATLPANTDKDVPVIGFLAHL
DTATDFTGKNVKPQIHENFDGNAITLNEELNVVLTPEQFPELPSYKGHTIITTDGTTLLGADDKAGLTEIMVAMNYLIHN
PQIKHGKVRVAFTPDEEIGRGPAHFDVEAFGASFAYTMDGGPLGGLEYESFNAAGAKLTFNGTNTHPGTAKNKMRNATKL
AMEFNGHLPVEEAPEYTEGYEGFYHLLSLNGDVEQSKAYYIIRDFDRKNFEARKNTIENIVKQMQEKYGQDAVVLEMNDQ
YYNMLEKIEPVREIVDIAYEAMKSLNIEPNIHPIRGGTDGSQLSYMGLPTPNIFTGGENYHGKFEYVSVDVMEKAVQVII
EIARRFEEQA
>Mature_410_residues
MKQELIERFTRYVKIDTQSNEDSHTVPTTPGQIEFGKLLVEELKEVGLTEVTMDDNGYVMATLPANTDKDVPVIGFLAHL
DTATDFTGKNVKPQIHENFDGNAITLNEELNVVLTPEQFPELPSYKGHTIITTDGTTLLGADDKAGLTEIMVAMNYLIHN
PQIKHGKVRVAFTPDEEIGRGPAHFDVEAFGASFAYTMDGGPLGGLEYESFNAAGAKLTFNGTNTHPGTAKNKMRNATKL
AMEFNGHLPVEEAPEYTEGYEGFYHLLSLNGDVEQSKAYYIIRDFDRKNFEARKNTIENIVKQMQEKYGQDAVVLEMNDQ
YYNMLEKIEPVREIVDIAYEAMKSLNIEPNIHPIRGGTDGSQLSYMGLPTPNIFTGGENYHGKFEYVSVDVMEKAVQVII
EIARRFEEQA

Specific function: Cleaves the N-terminal amino acid of tripeptides [H]

COG id: COG2195

COG function: function code E; Di- and tripeptidases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20B family [H]

Homologues:

Organism=Escherichia coli, GI1787372, Length=401, Percent_Identity=54.6134663341646, Blast_Score=439, Evalue=1e-124,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR002933
- InterPro:   IPR011650
- InterPro:   IPR010161 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.4.11.4 [H]

Molecular weight: Translated: 45852; Mature: 45852

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS00758 ARGE_DAPE_CPG2_1 ; PS00759 ARGE_DAPE_CPG2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQELIERFTRYVKIDTQSNEDSHTVPTTPGQIEFGKLLVEELKEVGLTEVTMDDNGYVM
CCHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCEEE
ATLPANTDKDVPVIGFLAHLDTATDFTGKNVKPQIHENFDGNAITLNEELNVVLTPEQFP
EEECCCCCCCCCCEEHHHHHCCCCCCCCCCCCCHHHCCCCCCEEEECCCCEEEECCHHCC
ELPSYKGHTIITTDGTTLLGADDKAGLTEIMVAMNYLIHNPQIKHGKVRVAFTPDEEIGR
CCCCCCCCEEEEECCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHCC
GPAHFDVEAFGASFAYTMDGGPLGGLEYESFNAAGAKLTFNGTNTHPGTAKNKMRNATKL
CCCCEEHHHCCCEEEEEECCCCCCCCEECCCCCCCCEEEECCCCCCCCCHHHHHHHHHEE
AMEFNGHLPVEEAPEYTEGYEGFYHLLSLNGDVEQSKAYYIIRDFDRKNFEARKNTIENI
EEEECCCCCCCCCCCCCHHHHHEEEEEECCCCCCCCCEEEEEEECCCCCHHHHHHHHHHH
VKQMQEKYGQDAVVLEMNDQYYNMLEKIEPVREIVDIAYEAMKSLNIEPNIHPIRGGTDG
HHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCC
SQLSYMGLPTPNIFTGGENYHGKFEYVSVDVMEKAVQVIIEIARRFEEQA
CCEEEECCCCCCEEECCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKQELIERFTRYVKIDTQSNEDSHTVPTTPGQIEFGKLLVEELKEVGLTEVTMDDNGYVM
CCHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCEEE
ATLPANTDKDVPVIGFLAHLDTATDFTGKNVKPQIHENFDGNAITLNEELNVVLTPEQFP
EEECCCCCCCCCCEEHHHHHCCCCCCCCCCCCCHHHCCCCCCEEEECCCCEEEECCHHCC
ELPSYKGHTIITTDGTTLLGADDKAGLTEIMVAMNYLIHNPQIKHGKVRVAFTPDEEIGR
CCCCCCCCEEEEECCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHCC
GPAHFDVEAFGASFAYTMDGGPLGGLEYESFNAAGAKLTFNGTNTHPGTAKNKMRNATKL
CCCCEEHHHCCCEEEEEECCCCCCCCEECCCCCCCCEEEECCCCCCCCCHHHHHHHHHEE
AMEFNGHLPVEEAPEYTEGYEGFYHLLSLNGDVEQSKAYYIIRDFDRKNFEARKNTIENI
EEEECCCCCCCCCCCCCHHHHHEEEEEECCCCCCCCCEEEEEEECCCCCHHHHHHHHHHH
VKQMQEKYGQDAVVLEMNDQYYNMLEKIEPVREIVDIAYEAMKSLNIEPNIHPIRGGTDG
HHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCC
SQLSYMGLPTPNIFTGGENYHGKFEYVSVDVMEKAVQVIIEIARRFEEQA
CCEEEECCCCCCEEECCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA