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The map label for this gene is exoA

Identifier: 49478354

GI number: 49478354

Start: 3568073

End: 3568831

Strand: Reverse

Name: exoA

Synonym: BT9727_3484

Alternate gene names: 49478354

Gene position: 3568831-3568073 (Counterclockwise)

Preceding gene: 49478357

Following gene: 49478353

Centisome position: 68.14

GC content: 35.44

Gene sequence:

>759_bases
GTGAAGTTCATTTCGTGGAATGTAAATGGTTTACGTGCAGTTATCGCAAAAGGTGGATTTTTAGAATATCTAGAGGAATC
AAATGCTGATATATTTTGTGTACAAGAGATTAAATTACAAGAGGGGCAAATTGATTTAAATGTAGAGGGATATTATACAT
ACTGGAATTATGCTGTGAAAAAAGGATATTCAGGGACGGCTATTTTTTCGAAAAAAGAACCGCTTTCTGTTACTTATGGT
TTAGGCATTGAAGAGCATGATCAAGAAGGACGAGTTATTACTTTAGAGTTCGAAGACTTTTACATCATTACGTTATATAC
ACCAAACTCCAAACGAGGATTAGAGCGTTTAGAGTACAGAATGAAATGGGAAGATGATTTCAGGGCCTATATTAAACGAT
TGGATGAGAAGAAATCAGTTGTTTTTTGCGGCGACTTAAACGTTGCCCATAAAGAAATCGATTTGAAAAATCCAAAAAGT
AATCGTAAAAACCCTGGATTCTCTGATGAGGAACGAGAGAAGTTTACATGTATTTTAGAAGAAGGATTTATTGATACGTA
TCGTTATCTATATCCTGATCAGGAAGGCGCATATTCGTGGTGGTCGTATCGAATGGGAGCAAGAGCAAAAAATATTGGAT
GGCGTTTAGATTATTTTGTTGTTTCCGAGAGAATGAAAGACCAAATAACAGCGGCGAAAATTAATAGTGAAGTAATGGGG
TCAGACCATTGCCCAGTTGAATTACATATAAATTTCTAA

Upstream 100 bases:

>100_bases
CATAAATGTAAGAGCAAGATTACAAAGGTGAAAATATGATTGTTTTTCTATTTTGTGATAGCATGAAATTTGGAAAGAAA
CATCAAGAGGAGGACTATAA

Downstream 100 bases:

>100_bases
AAAAAGAAGTATCCACCTAACATTAGGCGGATACTTCTTTTTTAATTCAGTTTGTTAAAATTTTTCAAATCTTTCTTGAA
ATAATTGCATATTAAATATA

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MKFISWNVNGLRAVIAKGGFLEYLEESNADIFCVQEIKLQEGQIDLNVEGYYTYWNYAVKKGYSGTAIFSKKEPLSVTYG
LGIEEHDQEGRVITLEFEDFYIITLYTPNSKRGLERLEYRMKWEDDFRAYIKRLDEKKSVVFCGDLNVAHKEIDLKNPKS
NRKNPGFSDEEREKFTCILEEGFIDTYRYLYPDQEGAYSWWSYRMGARAKNIGWRLDYFVVSERMKDQITAAKINSEVMG
SDHCPVELHINF

Sequences:

>Translated_252_residues
MKFISWNVNGLRAVIAKGGFLEYLEESNADIFCVQEIKLQEGQIDLNVEGYYTYWNYAVKKGYSGTAIFSKKEPLSVTYG
LGIEEHDQEGRVITLEFEDFYIITLYTPNSKRGLERLEYRMKWEDDFRAYIKRLDEKKSVVFCGDLNVAHKEIDLKNPKS
NRKNPGFSDEEREKFTCILEEGFIDTYRYLYPDQEGAYSWWSYRMGARAKNIGWRLDYFVVSERMKDQITAAKINSEVMG
SDHCPVELHINF
>Mature_252_residues
MKFISWNVNGLRAVIAKGGFLEYLEESNADIFCVQEIKLQEGQIDLNVEGYYTYWNYAVKKGYSGTAIFSKKEPLSVTYG
LGIEEHDQEGRVITLEFEDFYIITLYTPNSKRGLERLEYRMKWEDDFRAYIKRLDEKKSVVFCGDLNVAHKEIDLKNPKS
NRKNPGFSDEEREKFTCILEEGFIDTYRYLYPDQEGAYSWWSYRMGARAKNIGWRLDYFVVSERMKDQITAAKINSEVMG
SDHCPVELHINF

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=258, Percent_Identity=47.6744186046512, Blast_Score=266, Evalue=1e-71,
Organism=Homo sapiens, GI18375503, Length=258, Percent_Identity=47.6744186046512, Blast_Score=266, Evalue=1e-71,
Organism=Homo sapiens, GI18375501, Length=258, Percent_Identity=47.6744186046512, Blast_Score=266, Evalue=1e-71,
Organism=Homo sapiens, GI18375507, Length=310, Percent_Identity=30.9677419354839, Blast_Score=116, Evalue=2e-26,
Organism=Escherichia coli, GI1788046, Length=266, Percent_Identity=26.3157894736842, Blast_Score=97, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI71989536, Length=256, Percent_Identity=46.09375, Blast_Score=218, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI71989539, Length=145, Percent_Identity=37.9310344827586, Blast_Score=93, Evalue=1e-19,
Organism=Drosophila melanogaster, GI221330655, Length=255, Percent_Identity=50.9803921568627, Blast_Score=273, Evalue=5e-74,
Organism=Drosophila melanogaster, GI17136678, Length=255, Percent_Identity=50.9803921568627, Blast_Score=273, Evalue=8e-74,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29579; Mature: 29579

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00726 AP_NUCLEASE_F1_1 ; PS00728 AP_NUCLEASE_F1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFISWNVNGLRAVIAKGGFLEYLEESNADIFCVQEIKLQEGQIDLNVEGYYTYWNYAVK
CEEEEECCCCEEEEECCCCHHHHHHCCCCCEEEEEEEEECCCEEEEEEEEEEEEEEEEEC
KGYSGTAIFSKKEPLSVTYGLGIEEHDQEGRVITLEFEDFYIITLYTPNSKRGLERLEYR
CCCCCCEEEECCCCCEEEEECCCCCCCCCCCEEEEEECCEEEEEEECCCCHHHHHHHHEE
MKWEDDFRAYIKRLDEKKSVVFCGDLNVAHKEIDLKNPKSNRKNPGFSDEEREKFTCILE
ECCCHHHHHHHHHCCCCCCEEEEECCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEE
EGFIDTYRYLYPDQEGAYSWWSYRMGARAKNIGWRLDYFVVSERMKDQITAAKINSEVMG
CCCCHHHHEECCCCCCCCEEEEEECCCCCCCCCEEEEHEEEHHHHHHHHHHHHCCCHHCC
SDHCPVELHINF
CCCCCEEEEECC
>Mature Secondary Structure
MKFISWNVNGLRAVIAKGGFLEYLEESNADIFCVQEIKLQEGQIDLNVEGYYTYWNYAVK
CEEEEECCCCEEEEECCCCHHHHHHCCCCCEEEEEEEEECCCEEEEEEEEEEEEEEEEEC
KGYSGTAIFSKKEPLSVTYGLGIEEHDQEGRVITLEFEDFYIITLYTPNSKRGLERLEYR
CCCCCCEEEECCCCCEEEEECCCCCCCCCCCEEEEEECCEEEEEEECCCCHHHHHHHHEE
MKWEDDFRAYIKRLDEKKSVVFCGDLNVAHKEIDLKNPKSNRKNPGFSDEEREKFTCILE
ECCCHHHHHHHHHCCCCCCEEEEECCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEE
EGFIDTYRYLYPDQEGAYSWWSYRMGARAKNIGWRLDYFVVSERMKDQITAAKINSEVMG
CCCCHHHHEECCCCCCCCEEEEEECCCCCCCCCEEEEHEEEHHHHHHHHHHHHCCCHHCC
SDHCPVELHINF
CCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]