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The map label for this gene is trmD

Identifier: 49476237

GI number: 49476237

Start: 1824486

End: 1825184

Strand: Reverse

Name: trmD

Synonym: BH15820

Alternate gene names: 49476237

Gene position: 1825184-1824486 (Counterclockwise)

Preceding gene: 49476238

Following gene: 49476236

Centisome position: 94.52

GC content: 46.35

Gene sequence:

>699_bases
ATGAAATTTCAAGCACGTGTTTTAACACTTTATCCTGAAATGTTTCCTGGTTTTTTGGGTTGTTCTTTAGCAGGGCAAGC
TTTAAAGCAGGGGATATGGTCGCTTGAGACAGTGCAGATTAGGGATTTTGCTCTTGATAAACATCATAGCGTTGATGATA
CGCCTGCTGGTGGTGGTGCTGGTATGGTGATGCGGGCTGATGTGTTAGCGGCGGCACTGGATAGTTGTCCAAATGATTCC
CCAAGATTGTTAATGAGCCCGCGTGGGAGACTCTTGAATCAGGCTTATGCACGTTCTTTGGCACGGTCTTCGGGAGTGAC
ATTGGTTTGTGGGCGTTTTGAAGGAGTCGATGAGCGGATAATAGAAGCACGAGAATTAGAAGAAGTGTCCATTGGTGATT
ATATTCTTTCAGGGGGCGAAACGGCGGCACTGGTTCTTTTGGATGCTATTGTGCGTCTTCTTCCCGGTGTTATGGGTAAT
GAGATCTCCGCAAAGTGTGAAAGTTTTGAGAATGGTTTGCTTGAGCACCCTCAATATACGCGTCCTGCTGTTTTTGAAGG
GCGTGGTATTCCGCCAGTGTTGACGTCAGGTCATCACAAAGCTATCGCCAATTGGCGTCAACAGCAAGCCGAATCGTTAA
CACGGCAACGTCGCCCTGATCTTTACGCTCTTTATAATAAAAATCGTCAGAAAACTTGA

Upstream 100 bases:

>100_bases
ACAGATGAACAATTTTCCTTTGGGTAAATATCACAGTTTTTATAATACACTGGTTGAAGATGGTTTTATGTGACTTGAGA
TTAAAGGTGAGAATGAAAGA

Downstream 100 bases:

>100_bases
TTCATGTTGTAAGGTGTTGTATTGCATAGCTCTCTTTTTAAACAGTTTTCCTGTGAAGGGTTTTATTGAGTTCCAAAATA
AGAAACGGTGTATTCGCTCC

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 232; Mature: 232

Protein sequence:

>232_residues
MKFQARVLTLYPEMFPGFLGCSLAGQALKQGIWSLETVQIRDFALDKHHSVDDTPAGGGAGMVMRADVLAAALDSCPNDS
PRLLMSPRGRLLNQAYARSLARSSGVTLVCGRFEGVDERIIEARELEEVSIGDYILSGGETAALVLLDAIVRLLPGVMGN
EISAKCESFENGLLEHPQYTRPAVFEGRGIPPVLTSGHHKAIANWRQQQAESLTRQRRPDLYALYNKNRQKT

Sequences:

>Translated_232_residues
MKFQARVLTLYPEMFPGFLGCSLAGQALKQGIWSLETVQIRDFALDKHHSVDDTPAGGGAGMVMRADVLAAALDSCPNDS
PRLLMSPRGRLLNQAYARSLARSSGVTLVCGRFEGVDERIIEARELEEVSIGDYILSGGETAALVLLDAIVRLLPGVMGN
EISAKCESFENGLLEHPQYTRPAVFEGRGIPPVLTSGHHKAIANWRQQQAESLTRQRRPDLYALYNKNRQKT
>Mature_232_residues
MKFQARVLTLYPEMFPGFLGCSLAGQALKQGIWSLETVQIRDFALDKHHSVDDTPAGGGAGMVMRADVLAAALDSCPNDS
PRLLMSPRGRLLNQAYARSLARSSGVTLVCGRFEGVDERIIEARELEEVSIGDYILSGGETAALVLLDAIVRLLPGVMGN
EISAKCESFENGLLEHPQYTRPAVFEGRGIPPVLTSGHHKAIANWRQQQAESLTRQRRPDLYALYNKNRQKT

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=219, Percent_Identity=45.662100456621, Blast_Score=196, Evalue=1e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_BARHE (Q6G1R9)

Other databases:

- EMBL:   BX897699
- RefSeq:   YP_034278.1
- PDB:   3IEF
- PDBsum:   3IEF
- ProteinModelPortal:   Q6G1R9
- GeneID:   2865240
- GenomeReviews:   BX897699_GR
- KEGG:   bhe:BH15820
- NMPDR:   fig|283166.1.peg.1406
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- PhylomeDB:   Q6G1R9
- ProtClustDB:   PRK00026
- BioCyc:   BHEN283166:BH15820-MONOMER
- BRENDA:   2.1.1.31
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 25463; Mature: 25463

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: BINDING 111-111

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFQARVLTLYPEMFPGFLGCSLAGQALKQGIWSLETVQIRDFALDKHHSVDDTPAGGGA
CCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHCCCCCCCCCCCCCCC
GMVMRADVLAAALDSCPNDSPRLLMSPRGRLLNQAYARSLARSSGVTLVCGRFEGVDERI
CHHHHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHH
IEARELEEVSIGDYILSGGETAALVLLDAIVRLLPGVMGNEISAKCESFENGLLEHPQYT
HHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCC
RPAVFEGRGIPPVLTSGHHKAIANWRQQQAESLTRQRRPDLYALYNKNRQKT
CCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCC
>Mature Secondary Structure
MKFQARVLTLYPEMFPGFLGCSLAGQALKQGIWSLETVQIRDFALDKHHSVDDTPAGGGA
CCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHCCCCCCCCCCCCCCC
GMVMRADVLAAALDSCPNDSPRLLMSPRGRLLNQAYARSLARSSGVTLVCGRFEGVDERI
CHHHHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHH
IEARELEEVSIGDYILSGGETAALVLLDAIVRLLPGVMGNEISAKCESFENGLLEHPQYT
HHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCC
RPAVFEGRGIPPVLTSGHHKAIANWRQQQAESLTRQRRPDLYALYNKNRQKT
CCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA