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The map label for this gene is priA [H]

Identifier: 49476191

GI number: 49476191

Start: 1768572

End: 1770782

Strand: Reverse

Name: priA [H]

Synonym: BH15360

Alternate gene names: 49476191

Gene position: 1770782-1768572 (Counterclockwise)

Preceding gene: 49476193

Following gene: 49476190

Centisome position: 91.7

GC content: 47.4

Gene sequence:

>2211_bases
ATGACAGAGGGTGAGAGAGAGAAAAAGATTGTTTCGGTCTTGGTGCCTCTTCCTGTTGCGCATGCTTATAGTTATGAAGT
TCCTTCTTCTATGGAGTGTGAAGTTGGTTCTTTTGTTCGTGTTCCAGTGATGGGGCGACAAGTTTGTGGTTTTGTCGTTG
GCATTGGAGAGCAGACGGCAAAAGATAGGCAAAGTTCTGCACCGGTGGCGCATGACAAACTCCGTGTTCTCCTCCATGTT
TTTGACTGCCCACCATTAAAGACAGAGATGATTGCATTCTTGCGTTTTGTGAGTCGATACACAATGGCTCCTTTTGGTCT
TGTGGCACGATTGGTTTTGTCTGTGCCGGCTGCTTTAGAACCAGAAGCACAAATGCCAGGCTTGCGGTATTGTGGGGGGG
AAGTAGGGCGTTTAACACCAGCACGCCTAAGGGTTTTAGAATTGGCGCGCGATGGTGAGGTTTGGACACGTTCTGGTCTT
GCACATGCGGCAGGAACTTCTGTTTCTGTCGTTGAAGGGTTAAAATCGCTTGGAGTTTTTGAAGAGGTTATGATGCCGCC
ATCTGCGCTTGTGGCGCTGCCTGATCCGGATTTTGATCCGCCGGTGTTGGAGGGAGAACAGAGTGAGGCTGCGCGGCTTT
TGCGGGAAGGTGTCGTGTCTGCACAATTTCAAGTTTTTCTGCTTGATGGTGTGACGGGGTCAGGAAAGACGGAAGTGTAT
TTTGAAGCAGTTGCCCAAGCGCTTATAGAGGGCAAACAGGTTTTGATTTTATTGCCAGAAATTGCTTTAACACAACAATT
TTTAGATCGTTTTCATGCGCGTTTTGGAGTAGCCGCGGCAGAATGGCATTCCGATTTGGCACCACGTCGTCGGGAACGTA
TATGGCGGCAGGTGGCAGAAGGACGGGTGCGGGTTGTGGCTGGAGCACGTTCAGCACTTTTTCTCCCTTTTCATGAACTT
GGGTTGATTGTTGTCGATGAAGAACATGATGGTGCTTATAAACAAGAAGAGCGTATTTTTTATCATGCACGTGATATGGC
GGTTGCACGCGGCTCTTTTGAGCATTTTCCTGTTATTTTATCCTCAGCAACACCGTCGATTGAGAGCCAGGCAAATGTTT
TAAGAGGACGTTATCAAAAGGTGCATTTGCCATCACGTTTTAAAGAAGCAGCACTTCCACGGTTGCGGGTGGTTGATATG
CGTCAAGGAGGTGTGCAAAAGGGGCGTTTTATTTCCTTTGCTCTTGAAGAGGCTTTAAAACAAACACTCGAAAAAGGCGA
ACAAGCTCTCCTTTTTCTTAATCGTCGTGGCTATGCGCCTTTAACTTTATGCCGGGTTTGTGGTCATCGTTTTCATTGTA
GCGATTGTTCGAGTTGGTTGGTTGAGCATCGCGCGCAAGGGCAGCTTAAATGCCATCATTGTGGATATCATCAGTCTCTT
CCGGAAGCTTGTCCTGAATGTGGGACTCTCGATCATCTTGTCGCTTGTGGACCTGGCGTGGAAAGAATTGCGGAAGAAAC
ACGGACGCTTTTTCCACAGGCGCGACAATTGATTTTGTCAACGGATCTCAAAGGGGGGATTGGTCAATTGCGTCGTGAAT
TGGAAGCAATTGCAAAAGGTGATGTTGATATTCTTATCGGAACGCAGCTTGTTGCGAAAGGACACCATTTTCCTGGGCTT
TCTCTGGTTGGGGTGATTGATGCAGATCTTGGGCTTGCAAATGGCGATTTACGGGCGGGAGAGCGTACCTTTCAGCTTTT
GTCGCAGGTCACAGGAAGAGCAGGGCGTATGGGGTTGGAAAGTTTAGGTCTCTTGCAAACCTATCAACCTGATCATCCGG
TGATAAAAGCTTTGCTTTCGCAAAAAAGTGAGGATTTTTACACCTATGAGATTGCCATGCGTGAACATTATCATCTTCCG
CCTTATGGGCGTCTTGCCTCTTTGATTGTGTCTTCAGAAAAACGCCAAGCGGCAGAACATTACGCACGAGTGTTGCGCCA
AGTTGCACCAGGTGAAAAAAATATCTCTTTGATGGGCCCCGCGGAAGCTCCACTGGCTCTTATTCGAGGGCGTTATCGTT
TTCGGCTTTTGCTGCAAGGGCAACGTTCTTTTGATATACAAGGCTTTATTCGCGCGATGCTTGCAAATGCACCCAAAATA
CCTTCTTCCGTTCGGGTTCAAATTGATATTGATCCACAAAGTTTTCTTTAA

Upstream 100 bases:

>100_bases
AGGGGGGGCACAACGGATGATTTTTCAAAAATAGAAGTGCAAGGACAGAGCTGATATAGAAGGGGACTAGGAAGAGTTGG
TATAAAAGATGAAGTCGAAT

Downstream 100 bases:

>100_bases
AAAGATTGCTATAATGGGAAGGCATATTTGCTTAAAGGAGGCTTTTAAACGAGAGTCTGTCTTATTATCCTCTTTATTCT
CTGCTTTCTACGGACGATAA

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 736; Mature: 735

Protein sequence:

>736_residues
MTEGEREKKIVSVLVPLPVAHAYSYEVPSSMECEVGSFVRVPVMGRQVCGFVVGIGEQTAKDRQSSAPVAHDKLRVLLHV
FDCPPLKTEMIAFLRFVSRYTMAPFGLVARLVLSVPAALEPEAQMPGLRYCGGEVGRLTPARLRVLELARDGEVWTRSGL
AHAAGTSVSVVEGLKSLGVFEEVMMPPSALVALPDPDFDPPVLEGEQSEAARLLREGVVSAQFQVFLLDGVTGSGKTEVY
FEAVAQALIEGKQVLILLPEIALTQQFLDRFHARFGVAAAEWHSDLAPRRRERIWRQVAEGRVRVVAGARSALFLPFHEL
GLIVVDEEHDGAYKQEERIFYHARDMAVARGSFEHFPVILSSATPSIESQANVLRGRYQKVHLPSRFKEAALPRLRVVDM
RQGGVQKGRFISFALEEALKQTLEKGEQALLFLNRRGYAPLTLCRVCGHRFHCSDCSSWLVEHRAQGQLKCHHCGYHQSL
PEACPECGTLDHLVACGPGVERIAEETRTLFPQARQLILSTDLKGGIGQLRRELEAIAKGDVDILIGTQLVAKGHHFPGL
SLVGVIDADLGLANGDLRAGERTFQLLSQVTGRAGRMGLESLGLLQTYQPDHPVIKALLSQKSEDFYTYEIAMREHYHLP
PYGRLASLIVSSEKRQAAEHYARVLRQVAPGEKNISLMGPAEAPLALIRGRYRFRLLLQGQRSFDIQGFIRAMLANAPKI
PSSVRVQIDIDPQSFL

Sequences:

>Translated_736_residues
MTEGEREKKIVSVLVPLPVAHAYSYEVPSSMECEVGSFVRVPVMGRQVCGFVVGIGEQTAKDRQSSAPVAHDKLRVLLHV
FDCPPLKTEMIAFLRFVSRYTMAPFGLVARLVLSVPAALEPEAQMPGLRYCGGEVGRLTPARLRVLELARDGEVWTRSGL
AHAAGTSVSVVEGLKSLGVFEEVMMPPSALVALPDPDFDPPVLEGEQSEAARLLREGVVSAQFQVFLLDGVTGSGKTEVY
FEAVAQALIEGKQVLILLPEIALTQQFLDRFHARFGVAAAEWHSDLAPRRRERIWRQVAEGRVRVVAGARSALFLPFHEL
GLIVVDEEHDGAYKQEERIFYHARDMAVARGSFEHFPVILSSATPSIESQANVLRGRYQKVHLPSRFKEAALPRLRVVDM
RQGGVQKGRFISFALEEALKQTLEKGEQALLFLNRRGYAPLTLCRVCGHRFHCSDCSSWLVEHRAQGQLKCHHCGYHQSL
PEACPECGTLDHLVACGPGVERIAEETRTLFPQARQLILSTDLKGGIGQLRRELEAIAKGDVDILIGTQLVAKGHHFPGL
SLVGVIDADLGLANGDLRAGERTFQLLSQVTGRAGRMGLESLGLLQTYQPDHPVIKALLSQKSEDFYTYEIAMREHYHLP
PYGRLASLIVSSEKRQAAEHYARVLRQVAPGEKNISLMGPAEAPLALIRGRYRFRLLLQGQRSFDIQGFIRAMLANAPKI
PSSVRVQIDIDPQSFL
>Mature_735_residues
TEGEREKKIVSVLVPLPVAHAYSYEVPSSMECEVGSFVRVPVMGRQVCGFVVGIGEQTAKDRQSSAPVAHDKLRVLLHVF
DCPPLKTEMIAFLRFVSRYTMAPFGLVARLVLSVPAALEPEAQMPGLRYCGGEVGRLTPARLRVLELARDGEVWTRSGLA
HAAGTSVSVVEGLKSLGVFEEVMMPPSALVALPDPDFDPPVLEGEQSEAARLLREGVVSAQFQVFLLDGVTGSGKTEVYF
EAVAQALIEGKQVLILLPEIALTQQFLDRFHARFGVAAAEWHSDLAPRRRERIWRQVAEGRVRVVAGARSALFLPFHELG
LIVVDEEHDGAYKQEERIFYHARDMAVARGSFEHFPVILSSATPSIESQANVLRGRYQKVHLPSRFKEAALPRLRVVDMR
QGGVQKGRFISFALEEALKQTLEKGEQALLFLNRRGYAPLTLCRVCGHRFHCSDCSSWLVEHRAQGQLKCHHCGYHQSLP
EACPECGTLDHLVACGPGVERIAEETRTLFPQARQLILSTDLKGGIGQLRRELEAIAKGDVDILIGTQLVAKGHHFPGLS
LVGVIDADLGLANGDLRAGERTFQLLSQVTGRAGRMGLESLGLLQTYQPDHPVIKALLSQKSEDFYTYEIAMREHYHLPP
YGRLASLIVSSEKRQAAEHYARVLRQVAPGEKNISLMGPAEAPLALIRGRYRFRLLLQGQRSFDIQGFIRAMLANAPKIP
SSVRVQIDIDPQSFL

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=748, Percent_Identity=33.9572192513369, Blast_Score=388, Evalue=1e-109,
Organism=Escherichia coli, GI1787357, Length=122, Percent_Identity=35.2459016393443, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD [H]

EC number: NA

Molecular weight: Translated: 81373; Mature: 81242

Theoretical pI: Translated: 7.90; Mature: 7.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEGEREKKIVSVLVPLPVAHAYSYEVPSSMECEVGSFVRVPVMGRQVCGFVVGIGEQTA
CCCCHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCEEECCCHHHHHHHHHEECCCHHH
KDRQSSAPVAHDKLRVLLHVFDCPPLKTEMIAFLRFVSRYTMAPFGLVARLVLSVPAALE
HHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCC
PEAQMPGLRYCGGEVGRLTPARLRVLELARDGEVWTRSGLAHAAGTSVSVVEGLKSLGVF
CCCCCCCCHHCCCCCCCCCHHHHHHHHHHCCCCHHHHCCCHHHCCCCHHHHHHHHHHHHH
EEVMMPPSALVALPDPDFDPPVLEGEQSEAARLLREGVVSAQFQVFLLDGVTGSGKTEVY
HHHHCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCHHH
FEAVAQALIEGKQVLILLPEIALTQQFLDRFHARFGVAAAEWHSDLAPRRRERIWRQVAE
HHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHC
GRVRVVAGARSALFLPFHELGLIVVDEEHDGAYKQEERIFYHARDMAVARGSFEHFPVIL
CCEEEEECCCCEEEEEHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEE
SSATPSIESQANVLRGRYQKVHLPSRFKEAALPRLRVVDMRQGGVQKGRFISFALEEALK
ECCCCCHHHHHHHHHHHHHEECCCHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHH
QTLEKGEQALLFLNRRGYAPLTLCRVCGHRFHCSDCSSWLVEHRAQGQLKCHHCGYHQSL
HHHHCCCCEEEEEECCCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCHH
PEACPECGTLDHLVACGPGVERIAEETRTLFPQARQLILSTDLKGGIGQLRRELEAIAKG
HHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCC
DVDILIGTQLVAKGHHFPGLSLVGVIDADLGLANGDLRAGERTFQLLSQVTGRAGRMGLE
CEEEEECCHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCHHCCCHH
SLGLLQTYQPDHPVIKALLSQKSEDFYTYEIAMREHYHLPPYGRLASLIVSSEKRQAAEH
HHCHHHCCCCCHHHHHHHHHCCCCCEEEEEEEEHHCCCCCCHHHHHHHHHHHHHHHHHHH
YARVLRQVAPGEKNISLMGPAEAPLALIRGRYRFRLLLQGQRSFDIQGFIRAMLANAPKI
HHHHHHHHCCCCCCEEEECCCCCCHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCC
PSSVRVQIDIDPQSFL
CCCEEEEEECCCHHCC
>Mature Secondary Structure 
TEGEREKKIVSVLVPLPVAHAYSYEVPSSMECEVGSFVRVPVMGRQVCGFVVGIGEQTA
CCCHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCEEECCCHHHHHHHHHEECCCHHH
KDRQSSAPVAHDKLRVLLHVFDCPPLKTEMIAFLRFVSRYTMAPFGLVARLVLSVPAALE
HHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCC
PEAQMPGLRYCGGEVGRLTPARLRVLELARDGEVWTRSGLAHAAGTSVSVVEGLKSLGVF
CCCCCCCCHHCCCCCCCCCHHHHHHHHHHCCCCHHHHCCCHHHCCCCHHHHHHHHHHHHH
EEVMMPPSALVALPDPDFDPPVLEGEQSEAARLLREGVVSAQFQVFLLDGVTGSGKTEVY
HHHHCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCHHH
FEAVAQALIEGKQVLILLPEIALTQQFLDRFHARFGVAAAEWHSDLAPRRRERIWRQVAE
HHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHC
GRVRVVAGARSALFLPFHELGLIVVDEEHDGAYKQEERIFYHARDMAVARGSFEHFPVIL
CCEEEEECCCCEEEEEHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEE
SSATPSIESQANVLRGRYQKVHLPSRFKEAALPRLRVVDMRQGGVQKGRFISFALEEALK
ECCCCCHHHHHHHHHHHHHEECCCHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHH
QTLEKGEQALLFLNRRGYAPLTLCRVCGHRFHCSDCSSWLVEHRAQGQLKCHHCGYHQSL
HHHHCCCCEEEEEECCCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCHH
PEACPECGTLDHLVACGPGVERIAEETRTLFPQARQLILSTDLKGGIGQLRRELEAIAKG
HHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCC
DVDILIGTQLVAKGHHFPGLSLVGVIDADLGLANGDLRAGERTFQLLSQVTGRAGRMGLE
CEEEEECCHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCHHCCCHH
SLGLLQTYQPDHPVIKALLSQKSEDFYTYEIAMREHYHLPPYGRLASLIVSSEKRQAAEH
HHCHHHCCCCCHHHHHHHHHCCCCCEEEEEEEEHHCCCCCCHHHHHHHHHHHHHHHHHHH
YARVLRQVAPGEKNISLMGPAEAPLALIRGRYRFRLLLQGQRSFDIQGFIRAMLANAPKI
HHHHHHHHCCCCCCEEEECCCCCCHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCC
PSSVRVQIDIDPQSFL
CCCEEEEEECCCHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2861810 [H]