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The map label for this gene is pgk

Identifier: 49476164

GI number: 49476164

Start: 1728088

End: 1729281

Strand: Reverse

Name: pgk

Synonym: BH15070

Alternate gene names: 49476164

Gene position: 1729281-1728088 (Counterclockwise)

Preceding gene: 49476165

Following gene: 49476163

Centisome position: 89.55

GC content: 44.89

Gene sequence:

>1194_bases
ATGGGTTTTCGTACACTTGATGATGTTGATGTCACAGGAAAACGTGTTCTTGTGCGGGTGGATTTTAATGTACCAATGGC
GCAAGGAAAAGTTTGTGATGATACGCGTCTTAAACGACATAAAGAAACGCTCCTTGAATTGCAAAAGCGTGGTGCTAAGC
TTATTCTTCTTTCCCATTGTGGGCGCCCTAAAGGACAAGTGGAGCCAGAATTTTCACTCCATCCTGTTGTGCAAGTGCTG
GAAAAAATAATCAACCAACCCGTGAATTTTGCATCAGATTGTATTGGTGTAGCTGTACAGACAGCGGTTGAAGCATTGCA
AAATGGTGGTATTTTACTGCTTGAAAATGTTCGTTTTCATTCAGGTGAAGAAAAGAATGCGGGCTCTTTTGCTGAGGCTC
TAGCCCATAATGGAGATCTCTATGTCAATGATGCTTTTTCAGTCTCTCATCGTGCCCATGCTTCGGTAGAGGGGATAACA
CATTTTTTGCCTTCCTATGCGGGGCGTTCTTTACAATGTGAGTTGCAAGCATTGGAAAAAGGGCTTGGTAAGCCAGAGCG
TCCGGTGATCGCAATTGTTGGAGGGGCGAAGGTTTCCAGTAAGCTTTTTGTGCTCAATCATTTGGTTAAAAAGGTCGATT
ATTTGGTCATTGGTGGTGGCATGGCCAATAGTTTTTTAGCAGCGCAAGGTGTGTGTGTTGGAAAGTCATTGTGTGAACAT
GCGCTGATCGAAACGGTAAAAAAAGTTATTGAAAAAGCACGGGAGTGCCAATGTACGCTTCTGCTCCCTGTGGATGCAAT
CGTTGGATTTCGCTTTGAAAAAGATGCGCCACATCGTCTCTATGATATTGGAGATATTCCTGAGGATGGGATGATTTTGG
ATGTTGGTACGCGCTCCATTGCGCATATCAATGCCGTGATCGATAAAGCCGCTACCCTTGTGTGGAATGGACCTCTTGGT
GTTTTTGAGATGTCTCCTTTTGATCAAGGAACGATTGCAGTGGCACGCCATGCAGCAGAACTTAGTTTGACAGGGAAATT
GGTTTCAATTGCTGGAGGGGGAGATACGGTGTTTGCACTTAATCATGCTGGTGTTGCTGATGATTTTACTTATCTTTCAA
CCGCGGGTGGAGCCTTTTTGGAATGGATGGAAGGCAAGATGCTGCCTGGTATTCTAGCCCTCACGCAGGCTTGA

Upstream 100 bases:

>100_bases
AAAGGTGGCTTTTTTTGATGGAAGACGACGAGAAAATTGCGTAAGAAAAAAGTGAGCGGGTAGGTTACTTGAAACAACAG
AGAGTGAAAAGGGAGCAGTG

Downstream 100 bases:

>100_bases
AGGGATTCATTTGAACAAACAGTTAAAAAAAAGCTGTATGATTATGGAGGGTGTGCTGGTGCATTGCACGCTTTTGGAAA
TTAAGACAGGATAAAGAGGA

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MGFRTLDDVDVTGKRVLVRVDFNVPMAQGKVCDDTRLKRHKETLLELQKRGAKLILLSHCGRPKGQVEPEFSLHPVVQVL
EKIINQPVNFASDCIGVAVQTAVEALQNGGILLLENVRFHSGEEKNAGSFAEALAHNGDLYVNDAFSVSHRAHASVEGIT
HFLPSYAGRSLQCELQALEKGLGKPERPVIAIVGGAKVSSKLFVLNHLVKKVDYLVIGGGMANSFLAAQGVCVGKSLCEH
ALIETVKKVIEKARECQCTLLLPVDAIVGFRFEKDAPHRLYDIGDIPEDGMILDVGTRSIAHINAVIDKAATLVWNGPLG
VFEMSPFDQGTIAVARHAAELSLTGKLVSIAGGGDTVFALNHAGVADDFTYLSTAGGAFLEWMEGKMLPGILALTQA

Sequences:

>Translated_397_residues
MGFRTLDDVDVTGKRVLVRVDFNVPMAQGKVCDDTRLKRHKETLLELQKRGAKLILLSHCGRPKGQVEPEFSLHPVVQVL
EKIINQPVNFASDCIGVAVQTAVEALQNGGILLLENVRFHSGEEKNAGSFAEALAHNGDLYVNDAFSVSHRAHASVEGIT
HFLPSYAGRSLQCELQALEKGLGKPERPVIAIVGGAKVSSKLFVLNHLVKKVDYLVIGGGMANSFLAAQGVCVGKSLCEH
ALIETVKKVIEKARECQCTLLLPVDAIVGFRFEKDAPHRLYDIGDIPEDGMILDVGTRSIAHINAVIDKAATLVWNGPLG
VFEMSPFDQGTIAVARHAAELSLTGKLVSIAGGGDTVFALNHAGVADDFTYLSTAGGAFLEWMEGKMLPGILALTQA
>Mature_396_residues
GFRTLDDVDVTGKRVLVRVDFNVPMAQGKVCDDTRLKRHKETLLELQKRGAKLILLSHCGRPKGQVEPEFSLHPVVQVLE
KIINQPVNFASDCIGVAVQTAVEALQNGGILLLENVRFHSGEEKNAGSFAEALAHNGDLYVNDAFSVSHRAHASVEGITH
FLPSYAGRSLQCELQALEKGLGKPERPVIAIVGGAKVSSKLFVLNHLVKKVDYLVIGGGMANSFLAAQGVCVGKSLCEHA
LIETVKKVIEKARECQCTLLLPVDAIVGFRFEKDAPHRLYDIGDIPEDGMILDVGTRSIAHINAVIDKAATLVWNGPLGV
FEMSPFDQGTIAVARHAAELSLTGKLVSIAGGGDTVFALNHAGVADDFTYLSTAGGAFLEWMEGKMLPGILALTQA

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI4505763, Length=412, Percent_Identity=38.5922330097087, Blast_Score=262, Evalue=4e-70,
Organism=Homo sapiens, GI31543397, Length=412, Percent_Identity=37.378640776699, Blast_Score=253, Evalue=3e-67,
Organism=Escherichia coli, GI1789294, Length=399, Percent_Identity=42.6065162907268, Blast_Score=302, Evalue=2e-83,
Organism=Caenorhabditis elegans, GI17508823, Length=410, Percent_Identity=39.0243902439024, Blast_Score=280, Evalue=1e-75,
Organism=Saccharomyces cerevisiae, GI6319857, Length=410, Percent_Identity=38.2926829268293, Blast_Score=258, Evalue=2e-69,
Organism=Drosophila melanogaster, GI17136394, Length=411, Percent_Identity=38.6861313868613, Blast_Score=264, Evalue=9e-71,
Organism=Drosophila melanogaster, GI24581203, Length=413, Percent_Identity=37.772397094431, Blast_Score=261, Evalue=6e-70,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_BARHE (Q8L1Z8)

Other databases:

- EMBL:   AY074772
- EMBL:   BX897699
- RefSeq:   YP_034205.1
- ProteinModelPortal:   Q8L1Z8
- SMR:   Q8L1Z8
- GeneID:   2865020
- GenomeReviews:   BX897699_GR
- KEGG:   bhe:BH15070
- NMPDR:   fig|283166.1.peg.1333
- HOGENOM:   HBG453500
- OMA:   NCAIILP
- PhylomeDB:   Q8L1Z8
- ProtClustDB:   PRK00073
- BioCyc:   BHEN283166:BH15070-MONOMER
- BRENDA:   2.7.2.3
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 42618; Mature: 42487

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 36-36 BINDING 118-118 BINDING 151-151 BINDING 201-201 BINDING 323-323

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFRTLDDVDVTGKRVLVRVDFNVPMAQGKVCDDTRLKRHKETLLELQKRGAKLILLSHC
CCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECC
GRPKGQVEPEFSLHPVVQVLEKIINQPVNFASDCIGVAVQTAVEALQNGGILLLENVRFH
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCEEC
SGEEKNAGSFAEALAHNGDLYVNDAFSVSHRAHASVEGITHFLPSYAGRSLQCELQALEK
CCCCCCCHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHH
GLGKPERPVIAIVGGAKVSSKLFVLNHLVKKVDYLVIGGGMANSFLAAQGVCVGKSLCEH
HCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCEEEECCCCHHHHHHHCCHHHHHHHHHH
ALIETVKKVIEKARECQCTLLLPVDAIVGFRFEKDAPHRLYDIGDIPEDGMILDVGTRSI
HHHHHHHHHHHHHHCCEEEEEEEHHHHHCCEECCCCCCCEEECCCCCCCCEEEEECCCHH
AHINAVIDKAATLVWNGPLGVFEMSPFDQGTIAVARHAAELSLTGKLVSIAGGGDTVFAL
HHHHHHHHHHEEEEECCCCCEEEECCCCCCHHHHHHHHHHEEECCEEEEEECCCCEEEEE
NHAGVADDFTYLSTAGGAFLEWMEGKMLPGILALTQA
ECCCCCCCCHHHHCCCCHHHHHHCCCCCCCHHHCCCC
>Mature Secondary Structure 
GFRTLDDVDVTGKRVLVRVDFNVPMAQGKVCDDTRLKRHKETLLELQKRGAKLILLSHC
CCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECC
GRPKGQVEPEFSLHPVVQVLEKIINQPVNFASDCIGVAVQTAVEALQNGGILLLENVRFH
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCEEC
SGEEKNAGSFAEALAHNGDLYVNDAFSVSHRAHASVEGITHFLPSYAGRSLQCELQALEK
CCCCCCCHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHH
GLGKPERPVIAIVGGAKVSSKLFVLNHLVKKVDYLVIGGGMANSFLAAQGVCVGKSLCEH
HCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCEEEECCCCHHHHHHHCCHHHHHHHHHH
ALIETVKKVIEKARECQCTLLLPVDAIVGFRFEKDAPHRLYDIGDIPEDGMILDVGTRSI
HHHHHHHHHHHHHHCCEEEEEEEHHHHHCCEECCCCCCCEEECCCCCCCCEEEEECCCHH
AHINAVIDKAATLVWNGPLGVFEMSPFDQGTIAVARHAAELSLTGKLVSIAGGGDTVFAL
HHHHHHHHHHEEEEECCCCCEEEECCCCCCHHHHHHHHHHEEECCEEEEEECCCCEEEEE
NHAGVADDFTYLSTAGGAFLEWMEGKMLPGILALTQA
ECCCCCCCCHHHHCCCCHHHHHHCCCCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11983902