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The map label for this gene is clpB [H]

Identifier: 49476075

GI number: 49476075

Start: 1619874

End: 1622453

Strand: Reverse

Name: clpB [H]

Synonym: BH14110

Alternate gene names: 49476075

Gene position: 1622453-1619874 (Counterclockwise)

Preceding gene: 49476076

Following gene: 49476074

Centisome position: 84.02

GC content: 45.5

Gene sequence:

>2580_bases
ATGAATCTGGAAAAATATAGCGAACGGTTGCAAGGCTTTTTACAAACAGCACAAAATAAGGCTCTCTCTTCTGATCATCA
ACAATTTATGCCAGAACATTTCCTCAAAGTGTTGTTAGAGGATTCTCAAGGATTGGCCGCATCATTGATCCAAAAAGCTG
GCGGTGATCTCTCTCTTATTCAAAAAGCGCTCGAAGAAGCGCTCGATGCTTTGCCAAAAGTGCAAGGCGGAAATGGGCAA
CTTTATCTCTCGCAGCCGTTGGCAAAAGTCTTCACTCTAGCAGAAGATCTCGCACATAAAGCTGGGGATCAGTTTGTAAC
CGTCGAGCGTGTGCTGCAAGCGCTTATCATGGAAAAAACTGCAAAAACAGCCGATATTCTCACAAAAGCAGGTCTGACTC
CGCAAGCACTCAATCAAGCCATTAACGCTTTGCGTAAAGGAAAAACGGCTACAAGTCCTCATGCCGAAAGCCAATATGAT
GCTTTGCAAAAATATGCACGCGATCTTACAAAAGATGCACGGGAAGGAAAACTTGATCCTGTTATTGGACGTGAAGAAGA
AATTCGCCGCACTATTCAGGTGCTCTCACGGCGTACCAAAAATAATCCCGTGCTCATTGGTGAACCAGGTGTCGGAAAAA
CGGCAATTGTTGAAGGGTTAGCACTGCGTATTGTTAATGGCGATGTGCCAGAAACTTTGCGCGATAAACGGCTCTATGCA
CTCGATATGGGAGCACTTATCGCTGGTGCAAAATATCGCGGCGAATTCGAAGAACGTCTTAAAGCAGTGCTGGCTGAGGT
GCAAGCCGAAAATGGGCAGATCATTCTCTTTATTGATGAATTGCATAATCTGGTTGGTGCTGGAAAATCCGATGGTCCTA
TGGATGCTTCCAATTTGCTAAAACCCGCTCTTGCGCGCGGAGAACTCCATTGTGTGGGGGCAACAACACTTGATGAATAT
CGCAAATATGTCGAAAAAGATGCTGCTCTTGCACGGCGCTTCCAACCCGTCTTTGTGCCTGAACCAACCCTTGAGGATAC
AATCTCTATCTTGCGCGGCATTAAAGAAAAATATGAACAACATCATAAAGTGCGTTTGGCAGACAGCGCCTTAATTGCTG
CAGCCCGTTTGTCTAACCGTTATATTACTGATCGTTTCTTGCCAGATAAAGCCATTGATCTTATCGATGAAGCAGCGGCA
CGGTTGCGTATGCAAGTCGATTCAAAACCCGAAGAACTCGATGCACTTGATCGGAGAATCTTACAGTTGAAAATCGAACG
TGAAGCGTTGAAAACCGATGTAGAGCCAACAGCAAAAGAGCGTCTGAAAACCGTGCAAGCTGAACTCGAAACATTGGAAC
AACAATCGAGAGAAATGACAACCGCTTGGCAGGCTGAAAAACAAAAATTAGGACATGCTGCGGATTTGAAAAAACAGCTC
GAAGAAGCACGCAATGCTTTGGCTATTGCACAGCGTGATGGACAATTCCAAAAAGCCGGAGAACTCGCTTATAGCGTCAT
TCCGCAGCTCGAAAAACAATTGAGCATAGCTGAAAATGATGATCAGCAAAATCATCTCGTTGAAGAAACTGTTACCGCTG
AACATGTGGCCCGGATTGTTTCTCGATGGACCGGAGTTCCTGTTGATCGTATGCTTGAGGCAGAACGAGAGGCGCTCCTG
CGTATGGAAGACGAAATTAGCAAACGTGTGGTTGGACAGGGTGAAGCTGTGCAAGCTGTTTCCCGCGCTGTACGCCGTGC
GCGTGCCGGCCTGCAAGACCCTAATCGCCCCATCGGCTCCTTTATGTTTCTTGGTCCTACCGGTGTGGGAAAAACCGAAT
TAACCAAAGCGCTTGCGGCTTTTCTCTTTCAAGATCAAAATGCTATGTTGCGCATCGATATGTCGGAATATATGGAAAAA
CATGCCGGTGCACGCTTGATTGGGGCACCGCCCGGATATGTCGGATATGAAGAAGGGGGAGTGCTAACTGAAGCCGTGCG
GCGAAGACCTTATCAGGTCATTTTGTTCGATGAGATTGAAAAAGCCCATCCCGATATTTTTAATCTCTTGCTGCAGGTGC
TTGATGAAGGGCGCTTGACAGATAGCCAGGGACGTACTGTCGATTTTCGCAATACCTTACTGATTATGACCTCAAATTTG
GGAGCGGAATTTTTAACCGCCTTGCCAGAAGGACAAACGACCGATCAGGCAAAAAATGATGTCATGAATGTTGTAAAAGC
GGCTTTTCGTCCCGAATTTCTTAATCGTATTGATGAGGTTATTCTCTTTCAGAGATTACAACGTAGCGACATGGAAGCTA
TCGTTGATATCCAGATACAACATTTGCAAAATTTGCTCAATGAACGCAAAATAACTTTGCATATCGAACCAGAGGTTCGG
CAATTCCTTGGCGATAAGGGCTATGATCCTCTCTATGGCGCGCGGCCTCTCAAACGCATTATCCAAAGAGAAATTCAAGA
TCCACTGGCAGAAAACATCTTGTTTGGAAAAATTCATGATGAGATGGCGGTGACAATTGCAAAAGAAGGAGAGCATTTGA
CTTTTTTGCCTGAAAATTAA

Upstream 100 bases:

>100_bases
TTCATGCTGCCTCGTTCGCGGTGTTTTGCGGGGGTAAGGCCGATCCTTGCGGGTGCTTTGGGGAGTTAAAGTGCAGCGGT
AGTGCAAGAAAGGAAAAAAA

Downstream 100 bases:

>100_bases
GATACTGTTCTAGAAATGAGGAATATGGCAATGAAATAAAGATATAACAATGCGTTAAGAAATGCCTATCAGACAGAAAA
AAATGATAATAAAACATAAA

Product: ATP-dependent Clp protease, ATP-binding subunit clpB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 859; Mature: 859

Protein sequence:

>859_residues
MNLEKYSERLQGFLQTAQNKALSSDHQQFMPEHFLKVLLEDSQGLAASLIQKAGGDLSLIQKALEEALDALPKVQGGNGQ
LYLSQPLAKVFTLAEDLAHKAGDQFVTVERVLQALIMEKTAKTADILTKAGLTPQALNQAINALRKGKTATSPHAESQYD
ALQKYARDLTKDAREGKLDPVIGREEEIRRTIQVLSRRTKNNPVLIGEPGVGKTAIVEGLALRIVNGDVPETLRDKRLYA
LDMGALIAGAKYRGEFEERLKAVLAEVQAENGQIILFIDELHNLVGAGKSDGPMDASNLLKPALARGELHCVGATTLDEY
RKYVEKDAALARRFQPVFVPEPTLEDTISILRGIKEKYEQHHKVRLADSALIAAARLSNRYITDRFLPDKAIDLIDEAAA
RLRMQVDSKPEELDALDRRILQLKIEREALKTDVEPTAKERLKTVQAELETLEQQSREMTTAWQAEKQKLGHAADLKKQL
EEARNALAIAQRDGQFQKAGELAYSVIPQLEKQLSIAENDDQQNHLVEETVTAEHVARIVSRWTGVPVDRMLEAEREALL
RMEDEISKRVVGQGEAVQAVSRAVRRARAGLQDPNRPIGSFMFLGPTGVGKTELTKALAAFLFQDQNAMLRIDMSEYMEK
HAGARLIGAPPGYVGYEEGGVLTEAVRRRPYQVILFDEIEKAHPDIFNLLLQVLDEGRLTDSQGRTVDFRNTLLIMTSNL
GAEFLTALPEGQTTDQAKNDVMNVVKAAFRPEFLNRIDEVILFQRLQRSDMEAIVDIQIQHLQNLLNERKITLHIEPEVR
QFLGDKGYDPLYGARPLKRIIQREIQDPLAENILFGKIHDEMAVTIAKEGEHLTFLPEN

Sequences:

>Translated_859_residues
MNLEKYSERLQGFLQTAQNKALSSDHQQFMPEHFLKVLLEDSQGLAASLIQKAGGDLSLIQKALEEALDALPKVQGGNGQ
LYLSQPLAKVFTLAEDLAHKAGDQFVTVERVLQALIMEKTAKTADILTKAGLTPQALNQAINALRKGKTATSPHAESQYD
ALQKYARDLTKDAREGKLDPVIGREEEIRRTIQVLSRRTKNNPVLIGEPGVGKTAIVEGLALRIVNGDVPETLRDKRLYA
LDMGALIAGAKYRGEFEERLKAVLAEVQAENGQIILFIDELHNLVGAGKSDGPMDASNLLKPALARGELHCVGATTLDEY
RKYVEKDAALARRFQPVFVPEPTLEDTISILRGIKEKYEQHHKVRLADSALIAAARLSNRYITDRFLPDKAIDLIDEAAA
RLRMQVDSKPEELDALDRRILQLKIEREALKTDVEPTAKERLKTVQAELETLEQQSREMTTAWQAEKQKLGHAADLKKQL
EEARNALAIAQRDGQFQKAGELAYSVIPQLEKQLSIAENDDQQNHLVEETVTAEHVARIVSRWTGVPVDRMLEAEREALL
RMEDEISKRVVGQGEAVQAVSRAVRRARAGLQDPNRPIGSFMFLGPTGVGKTELTKALAAFLFQDQNAMLRIDMSEYMEK
HAGARLIGAPPGYVGYEEGGVLTEAVRRRPYQVILFDEIEKAHPDIFNLLLQVLDEGRLTDSQGRTVDFRNTLLIMTSNL
GAEFLTALPEGQTTDQAKNDVMNVVKAAFRPEFLNRIDEVILFQRLQRSDMEAIVDIQIQHLQNLLNERKITLHIEPEVR
QFLGDKGYDPLYGARPLKRIIQREIQDPLAENILFGKIHDEMAVTIAKEGEHLTFLPEN
>Mature_859_residues
MNLEKYSERLQGFLQTAQNKALSSDHQQFMPEHFLKVLLEDSQGLAASLIQKAGGDLSLIQKALEEALDALPKVQGGNGQ
LYLSQPLAKVFTLAEDLAHKAGDQFVTVERVLQALIMEKTAKTADILTKAGLTPQALNQAINALRKGKTATSPHAESQYD
ALQKYARDLTKDAREGKLDPVIGREEEIRRTIQVLSRRTKNNPVLIGEPGVGKTAIVEGLALRIVNGDVPETLRDKRLYA
LDMGALIAGAKYRGEFEERLKAVLAEVQAENGQIILFIDELHNLVGAGKSDGPMDASNLLKPALARGELHCVGATTLDEY
RKYVEKDAALARRFQPVFVPEPTLEDTISILRGIKEKYEQHHKVRLADSALIAAARLSNRYITDRFLPDKAIDLIDEAAA
RLRMQVDSKPEELDALDRRILQLKIEREALKTDVEPTAKERLKTVQAELETLEQQSREMTTAWQAEKQKLGHAADLKKQL
EEARNALAIAQRDGQFQKAGELAYSVIPQLEKQLSIAENDDQQNHLVEETVTAEHVARIVSRWTGVPVDRMLEAEREALL
RMEDEISKRVVGQGEAVQAVSRAVRRARAGLQDPNRPIGSFMFLGPTGVGKTELTKALAAFLFQDQNAMLRIDMSEYMEK
HAGARLIGAPPGYVGYEEGGVLTEAVRRRPYQVILFDEIEKAHPDIFNLLLQVLDEGRLTDSQGRTVDFRNTLLIMTSNL
GAEFLTALPEGQTTDQAKNDVMNVVKAAFRPEFLNRIDEVILFQRLQRSDMEAIVDIQIQHLQNLLNERKITLHIEPEVR
QFLGDKGYDPLYGARPLKRIIQREIQDPLAENILFGKIHDEMAVTIAKEGEHLTFLPEN

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=299, Percent_Identity=37.4581939799331, Blast_Score=199, Evalue=7e-51,
Organism=Escherichia coli, GI1788943, Length=839, Percent_Identity=57.5685339690107, Blast_Score=963, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=330, Percent_Identity=44.2424242424242, Blast_Score=293, Evalue=4e-80,
Organism=Saccharomyces cerevisiae, GI6320464, Length=713, Percent_Identity=54.1374474053296, Blast_Score=789, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323002, Length=861, Percent_Identity=42.6248548199768, Blast_Score=673, Evalue=0.0,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR017730
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 96061; Mature: 96061

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLEKYSERLQGFLQTAQNKALSSDHQQFMPEHFLKVLLEDSQGLAASLIQKAGGDLSLI
CCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHH
QKALEEALDALPKVQGGNGQLYLSQPLAKVFTLAEDLAHKAGDQFVTVERVLQALIMEKT
HHHHHHHHHHCCCCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHH
AKTADILTKAGLTPQALNQAINALRKGKTATSPHAESQYDALQKYARDLTKDAREGKLDP
HHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
VIGREEEIRRTIQVLSRRTKNNPVLIGEPGVGKTAIVEGLALRIVNGDVPETLRDKRLYA
CCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHEEECCCCCHHHHHCEEHH
LDMGALIAGAKYRGEFEERLKAVLAEVQAENGQIILFIDELHNLVGAGKSDGPMDASNLL
EEHHHHHHCCHHCCHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHCCCCCCCCCCHHHHH
KPALARGELHCVGATTLDEYRKYVEKDAALARRFQPVFVPEPTLEDTISILRGIKEKYEQ
HHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
HHKVRLADSALIAAARLSNRYITDRFLPDKAIDLIDEAAARLRMQVDSKPEELDALDRRI
HHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LQLKIEREALKTDVEPTAKERLKTVQAELETLEQQSREMTTAWQAEKQKLGHAADLKKQL
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
EEARNALAIAQRDGQFQKAGELAYSVIPQLEKQLSIAENDDQQNHLVEETVTAEHVARIV
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
SRWTGVPVDRMLEAEREALLRMEDEISKRVVGQGEAVQAVSRAVRRARAGLQDPNRPIGS
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
FMFLGPTGVGKTELTKALAAFLFQDQNAMLRIDMSEYMEKHAGARLIGAPPGYVGYEEGG
EEEECCCCCCHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHCCCEEECCCCCCCCCCCCC
VLTEAVRRRPYQVILFDEIEKAHPDIFNLLLQVLDEGRLTDSQGRTVDFRNTLLIMTSNL
HHHHHHHCCCCEEEEEHHHHHHCCHHHHHHHHHHHCCCCCCCCCCEEEECCEEEEEECCC
GAEFLTALPEGQTTDQAKNDVMNVVKAAFRPEFLNRIDEVILFQRLQRSDMEAIVDIQIQ
CHHHHHHCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HLQNLLNERKITLHIEPEVRQFLGDKGYDPLYGARPLKRIIQREIQDPLAENILFGKIHD
HHHHHHHCCEEEEEECHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHEEHH
EMAVTIAKEGEHLTFLPEN
HHEEEEECCCCEEEECCCC
>Mature Secondary Structure
MNLEKYSERLQGFLQTAQNKALSSDHQQFMPEHFLKVLLEDSQGLAASLIQKAGGDLSLI
CCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHH
QKALEEALDALPKVQGGNGQLYLSQPLAKVFTLAEDLAHKAGDQFVTVERVLQALIMEKT
HHHHHHHHHHCCCCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHH
AKTADILTKAGLTPQALNQAINALRKGKTATSPHAESQYDALQKYARDLTKDAREGKLDP
HHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
VIGREEEIRRTIQVLSRRTKNNPVLIGEPGVGKTAIVEGLALRIVNGDVPETLRDKRLYA
CCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHEEECCCCCHHHHHCEEHH
LDMGALIAGAKYRGEFEERLKAVLAEVQAENGQIILFIDELHNLVGAGKSDGPMDASNLL
EEHHHHHHCCHHCCHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHCCCCCCCCCCHHHHH
KPALARGELHCVGATTLDEYRKYVEKDAALARRFQPVFVPEPTLEDTISILRGIKEKYEQ
HHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
HHKVRLADSALIAAARLSNRYITDRFLPDKAIDLIDEAAARLRMQVDSKPEELDALDRRI
HHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LQLKIEREALKTDVEPTAKERLKTVQAELETLEQQSREMTTAWQAEKQKLGHAADLKKQL
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
EEARNALAIAQRDGQFQKAGELAYSVIPQLEKQLSIAENDDQQNHLVEETVTAEHVARIV
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
SRWTGVPVDRMLEAEREALLRMEDEISKRVVGQGEAVQAVSRAVRRARAGLQDPNRPIGS
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
FMFLGPTGVGKTELTKALAAFLFQDQNAMLRIDMSEYMEKHAGARLIGAPPGYVGYEEGG
EEEECCCCCCHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHCCCEEECCCCCCCCCCCCC
VLTEAVRRRPYQVILFDEIEKAHPDIFNLLLQVLDEGRLTDSQGRTVDFRNTLLIMTSNL
HHHHHHHCCCCEEEEEHHHHHHCCHHHHHHHHHHHCCCCCCCCCCEEEECCEEEEEECCC
GAEFLTALPEGQTTDQAKNDVMNVVKAAFRPEFLNRIDEVILFQRLQRSDMEAIVDIQIQ
CHHHHHHCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HLQNLLNERKITLHIEPEVRQFLGDKGYDPLYGARPLKRIIQREIQDPLAENILFGKIHD
HHHHHHHCCEEEEEECHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHEEHH
EMAVTIAKEGEHLTFLPEN
HHEEEEECCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]