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The map label for this gene is hisS

Identifier: 49476041

GI number: 49476041

Start: 1560810

End: 1562297

Strand: Reverse

Name: hisS

Synonym: BH13610

Alternate gene names: 49476041

Gene position: 1562297-1560810 (Counterclockwise)

Preceding gene: 49476042

Following gene: 49476040

Centisome position: 80.9

GC content: 43.21

Gene sequence:

>1488_bases
ATGTCATCGAAACAAGAAAAAACCAAAGCTCGTTTACCCCGTGGTTTTGTTGATCGCACAAGTGCACAATTATATGCAAT
CGAAGTTATGATTGCACAAATTCGTCAAGTTTATGAACTTTACGGTTTTGAAGCCCTGGAAACACCAATTTTTGAATATA
CCGATGTGTTAGGCAAGTTTTTACCTGATGAGGATAGGCCAAATGCTGGGGTTTTTTCTCTACAAGATGATGATGAACAG
TGGATGTCTTTGCGTTATGATCTCACTGCACCTCTTGCCCGTTATTTTTCAGAAAATTTTGAAACTTTGCCAAAGCCTTA
TCGGAGTTATCGTCTAGGCTTTGTTTTTCGCAATGAAAAACCTGGACCGGGACGTTTTCGGCAATTTATGCAATTTGATG
CTGATATTGTGGGAACGCCGACAGTTGCTGCAGATGCCGAAATTTGCATGATGGCAGCCGATAGTTTGCAAAAATTAGGA
TTTCAGCATCATGATTATGTCATTCGTTTGAATAACCGGAAAATTCTGGATGCTGTTTTGGAGAAAGTTGGCTTGGCGGG
AAGTGAGCAGGCTCAAAGGCGCTTAACCGTTCTGAGAGCAATTGATAAACTTGATAAATTTGGTCTTGAAGGTGTACGTT
TGCTTTTGGGAAAAGGGCGTTTGGATGAAAGTGGTGATTTCACAAAAGGCGCAGAGCTTAAGGACAAAGAGATTGATGGT
ATTCTTTCCTTGCTTACCATAGAGGTTGGAACTGCAGAGGAAACGTTTGACGCTCTTAGAAAAATCGTTGACCAGAGTGA
GGAAGGGCTTGAAGGGGTTCGCGAACTTGAGGAGATGCAGGCAATTTTTGCTGAAAATGACACTCAAAACCGTATCAAAA
TTGATCCCTCAGTTGTGCGTGGATTGGAATATTATACGGGACCTGTTTTTGAGGCAGCATTGCTTTTTGATGTTCTCAAT
GATGATGGCCAAAAAGTTGTTTTTGGCTCCGTTGGTGGAGGCGGGCGCTATGATGGGTTGGTGGCACGCTTTCGTGGTGA
AAATATTCCAGCAACAGGTTTCTCAATTGGTGTTTCACGCTTGATAGCTGCTTTGCAAAATCTTGGAAAATTGCCTGTAA
AAGAAAAGACTGGGCCTGTTGTGGTGCTGATGATGGATAAAGAACCAGAAATTGTTGCACGCTATCAAAAAATGGTCATG
CAGTTGCGTAGTGCGGGGATTCCTGCTGAACTCTATTTGGGGGCATCAGGGATGAAAGCACAGATGAAATACGCGGATCG
CCGTCAGGCGCCTTGTGTGGTGATCCAAGGTTCTCAAGAGCGCGAGAGTGGAACAATACAGATCAAAGATTTGGTTGAAG
GGGCACGTTTGTCTCATGAAATTAAGGATAATCAAACATGGCGTGAAAGCCGACCAGCGCAAGTGACGGTTGATGAAGAG
CAATTGGTCAAAACGGTACAAGATATTTTGGCTGCTCAAAAACGGTAA

Upstream 100 bases:

>100_bases
TGTTGCCAAAGCACTCAGATTGATAAAGTCATTGCAGCGTTTGTTTCTATAAGATAAGACACATATTATCTGTGAACATA
AATTGTTGCTAGAGAATAAC

Downstream 100 bases:

>100_bases
GAGAACAGTTTTTAGACAAGAGATTGTTTAGTAGATTTCGGAAATTCTCAATTCAATTTTCAAAATCTGTATTTTGCTTG
TGCAGATATGCTTACGGACA

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 495; Mature: 494

Protein sequence:

>495_residues
MSSKQEKTKARLPRGFVDRTSAQLYAIEVMIAQIRQVYELYGFEALETPIFEYTDVLGKFLPDEDRPNAGVFSLQDDDEQ
WMSLRYDLTAPLARYFSENFETLPKPYRSYRLGFVFRNEKPGPGRFRQFMQFDADIVGTPTVAADAEICMMAADSLQKLG
FQHHDYVIRLNNRKILDAVLEKVGLAGSEQAQRRLTVLRAIDKLDKFGLEGVRLLLGKGRLDESGDFTKGAELKDKEIDG
ILSLLTIEVGTAEETFDALRKIVDQSEEGLEGVRELEEMQAIFAENDTQNRIKIDPSVVRGLEYYTGPVFEAALLFDVLN
DDGQKVVFGSVGGGGRYDGLVARFRGENIPATGFSIGVSRLIAALQNLGKLPVKEKTGPVVVLMMDKEPEIVARYQKMVM
QLRSAGIPAELYLGASGMKAQMKYADRRQAPCVVIQGSQERESGTIQIKDLVEGARLSHEIKDNQTWRESRPAQVTVDEE
QLVKTVQDILAAQKR

Sequences:

>Translated_495_residues
MSSKQEKTKARLPRGFVDRTSAQLYAIEVMIAQIRQVYELYGFEALETPIFEYTDVLGKFLPDEDRPNAGVFSLQDDDEQ
WMSLRYDLTAPLARYFSENFETLPKPYRSYRLGFVFRNEKPGPGRFRQFMQFDADIVGTPTVAADAEICMMAADSLQKLG
FQHHDYVIRLNNRKILDAVLEKVGLAGSEQAQRRLTVLRAIDKLDKFGLEGVRLLLGKGRLDESGDFTKGAELKDKEIDG
ILSLLTIEVGTAEETFDALRKIVDQSEEGLEGVRELEEMQAIFAENDTQNRIKIDPSVVRGLEYYTGPVFEAALLFDVLN
DDGQKVVFGSVGGGGRYDGLVARFRGENIPATGFSIGVSRLIAALQNLGKLPVKEKTGPVVVLMMDKEPEIVARYQKMVM
QLRSAGIPAELYLGASGMKAQMKYADRRQAPCVVIQGSQERESGTIQIKDLVEGARLSHEIKDNQTWRESRPAQVTVDEE
QLVKTVQDILAAQKR
>Mature_494_residues
SSKQEKTKARLPRGFVDRTSAQLYAIEVMIAQIRQVYELYGFEALETPIFEYTDVLGKFLPDEDRPNAGVFSLQDDDEQW
MSLRYDLTAPLARYFSENFETLPKPYRSYRLGFVFRNEKPGPGRFRQFMQFDADIVGTPTVAADAEICMMAADSLQKLGF
QHHDYVIRLNNRKILDAVLEKVGLAGSEQAQRRLTVLRAIDKLDKFGLEGVRLLLGKGRLDESGDFTKGAELKDKEIDGI
LSLLTIEVGTAEETFDALRKIVDQSEEGLEGVRELEEMQAIFAENDTQNRIKIDPSVVRGLEYYTGPVFEAALLFDVLND
DGQKVVFGSVGGGGRYDGLVARFRGENIPATGFSIGVSRLIAALQNLGKLPVKEKTGPVVVLMMDKEPEIVARYQKMVMQ
LRSAGIPAELYLGASGMKAQMKYADRRQAPCVVIQGSQERESGTIQIKDLVEGARLSHEIKDNQTWRESRPAQVTVDEEQ
LVKTVQDILAAQKR

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI6996014, Length=462, Percent_Identity=30.952380952381, Blast_Score=182, Evalue=6e-46,
Organism=Homo sapiens, GI15029520, Length=467, Percent_Identity=29.9785867237687, Blast_Score=179, Evalue=6e-45,
Organism=Escherichia coli, GI1788861, Length=459, Percent_Identity=25.7080610021786, Blast_Score=110, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71993693, Length=495, Percent_Identity=29.8989898989899, Blast_Score=177, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI71993686, Length=495, Percent_Identity=29.8989898989899, Blast_Score=177, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6325290, Length=518, Percent_Identity=27.7992277992278, Blast_Score=153, Evalue=6e-38,
Organism=Drosophila melanogaster, GI24643061, Length=478, Percent_Identity=30.7531380753138, Blast_Score=180, Evalue=2e-45,
Organism=Drosophila melanogaster, GI24643059, Length=497, Percent_Identity=29.5774647887324, Blast_Score=169, Evalue=5e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_BARHE (Q6G289)

Other databases:

- EMBL:   BX897699
- RefSeq:   YP_034082.1
- ProteinModelPortal:   Q6G289
- GeneID:   2865355
- GenomeReviews:   BX897699_GR
- KEGG:   bhe:BH13610
- NMPDR:   fig|283166.1.peg.1210
- HOGENOM:   HBG616575
- OMA:   IGKVFRD
- PhylomeDB:   Q6G289
- ProtClustDB:   PRK00037
- BioCyc:   BHEN283166:BH13610-MONOMER
- BRENDA:   6.1.1.21
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 55480; Mature: 55349

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSKQEKTKARLPRGFVDRTSAQLYAIEVMIAQIRQVYELYGFEALETPIFEYTDVLGKF
CCCCHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHH
LPDEDRPNAGVFSLQDDDEQWMSLRYDLTAPLARYFSENFETLPKPYRSYRLGFVFRNEK
CCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCEEEEEEEECCC
PGPGRFRQFMQFDADIVGTPTVAADAEICMMAADSLQKLGFQHHDYVIRLNNRKILDAVL
CCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHH
EKVGLAGSEQAQRRLTVLRAIDKLDKFGLEGVRLLLGKGRLDESGDFTKGAELKDKEIDG
HHHCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHH
ILSLLTIEVGTAEETFDALRKIVDQSEEGLEGVRELEEMQAIFAENDTQNRIKIDPSVVR
HHHHHHEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHH
GLEYYTGPVFEAALLFDVLNDDGQKVVFGSVGGGGRYDGLVARFRGENIPATGFSIGVSR
HHHHHCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHEECCCCCCCCHHHHHHHH
LIAALQNLGKLPVKEKTGPVVVLMMDKEPEIVARYQKMVMQLRSAGIPAELYLGASGMKA
HHHHHHHCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCHH
QMKYADRRQAPCVVIQGSQERESGTIQIKDLVEGARLSHEIKDNQTWRESRPAQVTVDEE
HHHHHHCCCCCEEEEECCCCCCCCCEEEHHHHHHHHHHHHHHCCCCHHHCCCCEEEECHH
QLVKTVQDILAAQKR
HHHHHHHHHHHHCCC
>Mature Secondary Structure 
SSKQEKTKARLPRGFVDRTSAQLYAIEVMIAQIRQVYELYGFEALETPIFEYTDVLGKF
CCCHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHH
LPDEDRPNAGVFSLQDDDEQWMSLRYDLTAPLARYFSENFETLPKPYRSYRLGFVFRNEK
CCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCEEEEEEEECCC
PGPGRFRQFMQFDADIVGTPTVAADAEICMMAADSLQKLGFQHHDYVIRLNNRKILDAVL
CCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHH
EKVGLAGSEQAQRRLTVLRAIDKLDKFGLEGVRLLLGKGRLDESGDFTKGAELKDKEIDG
HHHCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHH
ILSLLTIEVGTAEETFDALRKIVDQSEEGLEGVRELEEMQAIFAENDTQNRIKIDPSVVR
HHHHHHEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHH
GLEYYTGPVFEAALLFDVLNDDGQKVVFGSVGGGGRYDGLVARFRGENIPATGFSIGVSR
HHHHHCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHEECCCCCCCCHHHHHHHH
LIAALQNLGKLPVKEKTGPVVVLMMDKEPEIVARYQKMVMQLRSAGIPAELYLGASGMKA
HHHHHHHCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCHH
QMKYADRRQAPCVVIQGSQERESGTIQIKDLVEGARLSHEIKDNQTWRESRPAQVTVDEE
HHHHHHCCCCCEEEEECCCCCCCCCEEEHHHHHHHHHHHHHHCCCCHHHCCCCEEEECHH
QLVKTVQDILAAQKR
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA