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The map label for this gene is amiD [H]

Identifier: 49475962

GI number: 49475962

Start: 1393575

End: 1394534

Strand: Reverse

Name: amiD [H]

Synonym: BH12510

Alternate gene names: 49475962

Gene position: 1394534-1393575 (Counterclockwise)

Preceding gene: 49475963

Following gene: 49475951

Centisome position: 72.22

GC content: 39.69

Gene sequence:

>960_bases
ATGTATCAGATTGATTACAACAGCTATCGTTCTGTAAAGAGCTTTAATCGTCGTATTCGTTTTCTTGTGATGCATTATAC
TTCACTTGATTTTAAAGCATCGATCATGGCTCTTACAGGAGAGAGGGTTAGTGCACATTATCTTGTTCCTGATCCATCAG
AACAGACCTATATTGAGGCAGGATTTAAGGATATGCGTATCTTTAATCTTGTTGATGAAAACGAGCGTGCGTGGCATGCA
GGCGTTAGTTCATGGGCTGGGCGTAGTAATTTAAATGATACGTCTCTTGGAATTGAAATAGTCAATTTAGCAACTGGACA
TTCTGATTCTTTAGAGGAGATATGCGCTGAAGCAGCTTTAAAGGATATGCGGGTTTTAAATCCAGTTCATGAAGGTGGGG
GATCCTTGCAAACAGATGTTGGTTCATTAGCTGATGGCAGTCATGTTAGTGGTTTCGCGTTTCCTCTTGAAACAGTTAAT
GTGGTGACTTATAGTGATGAGGTATTTACATTTCCTCCCTACAATCCAACGCAAATTGATGCAGTTAAGGAGCTTGCGTT
AAATATTCTTCAGCGATATCCTGATATTACACCAACCGATGTTGTGGGGCACAGTGATATTGCACTTGGAAGAAAGAGTG
ATCCAGGTGCTGCTTTTCCTTGGAAAGAACTTTATGCAGCAGGCATAGGGGCATGGTATGATGATGAAGTAAAGGATCAC
TATCAGGAGCAGTTTTGTAAGAGTTTTCCACTCAAAGCAGATGTTTTGGCTAAATTGAGATGTTATGGTTACGACATTTC
GGCTGCATGTAGCGAAATAGGATATAAAGACTTAATCCGAGCATTTCAGCTTCATTTTCGTCAAGAAAATTATGATGGAA
TTTTAGATGTTGAAACAGCCGCGATTATTTATGCATTGGTAGATAAATATTTTCCGTCGAACGCTTTCAATGTGACGTGA

Upstream 100 bases:

>100_bases
TATTGTAAAAATTTTCAAAAATAATACCAAGGTTATTTTCCTGTGCAGCCTGTTGTGATGTGTGGTGGTCTGTGTAAATG
AGATAGAAAGGAAGTTTTTT

Downstream 100 bases:

>100_bases
TTTAAATATATATCATACAACAATGAGTGTTGTCTTTTTTGAAGGATATTTTTCTTGGTTTTTGTGTGTAAACTCTGGGT
GAGAAAAGGAGGATTATTTA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MYQIDYNSYRSVKSFNRRIRFLVMHYTSLDFKASIMALTGERVSAHYLVPDPSEQTYIEAGFKDMRIFNLVDENERAWHA
GVSSWAGRSNLNDTSLGIEIVNLATGHSDSLEEICAEAALKDMRVLNPVHEGGGSLQTDVGSLADGSHVSGFAFPLETVN
VVTYSDEVFTFPPYNPTQIDAVKELALNILQRYPDITPTDVVGHSDIALGRKSDPGAAFPWKELYAAGIGAWYDDEVKDH
YQEQFCKSFPLKADVLAKLRCYGYDISAACSEIGYKDLIRAFQLHFRQENYDGILDVETAAIIYALVDKYFPSNAFNVT

Sequences:

>Translated_319_residues
MYQIDYNSYRSVKSFNRRIRFLVMHYTSLDFKASIMALTGERVSAHYLVPDPSEQTYIEAGFKDMRIFNLVDENERAWHA
GVSSWAGRSNLNDTSLGIEIVNLATGHSDSLEEICAEAALKDMRVLNPVHEGGGSLQTDVGSLADGSHVSGFAFPLETVN
VVTYSDEVFTFPPYNPTQIDAVKELALNILQRYPDITPTDVVGHSDIALGRKSDPGAAFPWKELYAAGIGAWYDDEVKDH
YQEQFCKSFPLKADVLAKLRCYGYDISAACSEIGYKDLIRAFQLHFRQENYDGILDVETAAIIYALVDKYFPSNAFNVT
>Mature_319_residues
MYQIDYNSYRSVKSFNRRIRFLVMHYTSLDFKASIMALTGERVSAHYLVPDPSEQTYIEAGFKDMRIFNLVDENERAWHA
GVSSWAGRSNLNDTSLGIEIVNLATGHSDSLEEICAEAALKDMRVLNPVHEGGGSLQTDVGSLADGSHVSGFAFPLETVN
VVTYSDEVFTFPPYNPTQIDAVKELALNILQRYPDITPTDVVGHSDIALGRKSDPGAAFPWKELYAAGIGAWYDDEVKDH
YQEQFCKSFPLKADVLAKLRCYGYDISAACSEIGYKDLIRAFQLHFRQENYDGILDVETAAIIYALVDKYFPSNAFNVT

Specific function: Unknown

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787092, Length=141, Percent_Identity=43.2624113475177, Blast_Score=109, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 35705; Mature: 35705

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYQIDYNSYRSVKSFNRRIRFLVMHYTSLDFKASIMALTGERVSAHYLVPDPSEQTYIEA
CEECCCHHHHHHHHHHHHEEEEEEEEECCCCCEEEEEEECCCCEEEEECCCCCCCCEECC
GFKDMRIFNLVDENERAWHAGVSSWAGRSNLNDTSLGIEIVNLATGHSDSLEEICAEAAL
CCCCEEEEEEECCCCCHHHHCHHHHCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHH
KDMRVLNPVHEGGGSLQTDVGSLADGSHVSGFAFPLETVNVVTYSDEVFTFPPYNPTQID
HHHHHHHHHHHCCCCCHHCHHCCCCCCCCCCEEECCCEEEEEEECCCEEECCCCCCHHHH
AVKELALNILQRYPDITPTDVVGHSDIALGRKSDPGAAFPWKELYAAGIGAWYDDEVKDH
HHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHH
YQEQFCKSFPLKADVLAKLRCYGYDISAACSEIGYKDLIRAFQLHFRQENYDGILDVETA
HHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEHHHH
AIIYALVDKYFPSNAFNVT
HHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MYQIDYNSYRSVKSFNRRIRFLVMHYTSLDFKASIMALTGERVSAHYLVPDPSEQTYIEA
CEECCCHHHHHHHHHHHHEEEEEEEEECCCCCEEEEEEECCCCEEEEECCCCCCCCEECC
GFKDMRIFNLVDENERAWHAGVSSWAGRSNLNDTSLGIEIVNLATGHSDSLEEICAEAAL
CCCCEEEEEEECCCCCHHHHCHHHHCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHH
KDMRVLNPVHEGGGSLQTDVGSLADGSHVSGFAFPLETVNVVTYSDEVFTFPPYNPTQID
HHHHHHHHHHHCCCCCHHCHHCCCCCCCCCCEEECCCEEEEEEECCCEEECCCCCCHHHH
AVKELALNILQRYPDITPTDVVGHSDIALGRKSDPGAAFPWKELYAAGIGAWYDDEVKDH
HHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHH
YQEQFCKSFPLKADVLAKLRCYGYDISAACSEIGYKDLIRAFQLHFRQENYDGILDVETA
HHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEHHHH
AIIYALVDKYFPSNAFNVT
HHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]