Click here to switch to the map view.

The map label for this gene is yafC [H]

Identifier: 49475889

GI number: 49475889

Start: 1295556

End: 1296461

Strand: Reverse

Name: yafC [H]

Synonym: BH11650

Alternate gene names: 49475889

Gene position: 1296461-1295556 (Counterclockwise)

Preceding gene: 49475890

Following gene: 49475879

Centisome position: 67.14

GC content: 39.85

Gene sequence:

>906_bases
GTGTCGTCACCATTGGATTGGGATAAGTTAAGGGTTTTTCATGCGGCGGCAGAAGCGGGTTCTTTTACCCATGCTGCTCA
AATGCTTCATTTGTCCCAATCAGCAATTTCACGACAGGTTTCAGCTTTAGAACAGGAGGTTGGCGTGTCCCTGTTCCAAC
GTCATGCACGTGGTTTGATTCTAACGGAGCAGGGTGAGATTCTCTATCGTACAACGCATGATGTTTTGATGAAACTTGAA
AGTGCTCGCTCCCAATTAAGTGAAAGCTGTGAAAAACCTGCAGGCAATTTGCGTGTTACATCGACCTTCGGAATGGGAGC
AGGATGGCTTGTGGAGCGCATGCCTGAATTCCTTAGTCTCTATCCGGATATGCGGATTCAGCTCTTGTTTGATGATAAAG
AACTTGACCTGATGATGCGTTATGCAGATTGTGCTGTTCGTTTACAACAGCCTCAGCAGCCTGATCTTATACAAAAAAAA
TTATTTACAATTCATATGCATGTTTATGCTTCAAAAAATTATATTGCAAATTATGGCAAACCAGAAAAATTGTCTGAACT
GGATAACCACCGTATTATTTCATTTGGTGAACCTGTTCCAACTTATTTGTCTGGTTTAAATTGGTTGGAAAAGGCTGGTA
AAAGCGATGGGGCTTTGCGTCTTTCAATATTGCGAATCAATAATATCATTTCAATCAAGAATGCTCTTATGCGTGATCTT
GGTATTGCTGTGCTTCCTGATTATATCGTGGATAATGATGAGCACCTTGTCCGTCTTTTTCCTGATATGGTTGATATTCC
TTCTTTTGATACTTTTTTTTGTTATCCTTATGCTTTAAAGAATTTGGCAAAGCTAAAAGCTTTTCGAGATTATATTTTTT
CAAAAGCCCGTCAGTGGTCTTTTTAA

Upstream 100 bases:

>100_bases
TGGCAGCATTGGAGGCAGAACGATTTTTGGAGTTGCAGATATAGACGTCAAAAATAGATATCTATCGGAAAAGTGAAAAA
AGTAAGAGGAAAAATTAATT

Downstream 100 bases:

>100_bases
TAATGTGTTCTTTTAAGCTATGTGTTTAATGCATAGCAGATACGCTGAAGTCTTTATTGTACTTCTTCTTATAAAATGAC
ATACAATAAGATCTCTTGAT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 300

Protein sequence:

>301_residues
MSSPLDWDKLRVFHAAAEAGSFTHAAQMLHLSQSAISRQVSALEQEVGVSLFQRHARGLILTEQGEILYRTTHDVLMKLE
SARSQLSESCEKPAGNLRVTSTFGMGAGWLVERMPEFLSLYPDMRIQLLFDDKELDLMMRYADCAVRLQQPQQPDLIQKK
LFTIHMHVYASKNYIANYGKPEKLSELDNHRIISFGEPVPTYLSGLNWLEKAGKSDGALRLSILRINNIISIKNALMRDL
GIAVLPDYIVDNDEHLVRLFPDMVDIPSFDTFFCYPYALKNLAKLKAFRDYIFSKARQWSF

Sequences:

>Translated_301_residues
MSSPLDWDKLRVFHAAAEAGSFTHAAQMLHLSQSAISRQVSALEQEVGVSLFQRHARGLILTEQGEILYRTTHDVLMKLE
SARSQLSESCEKPAGNLRVTSTFGMGAGWLVERMPEFLSLYPDMRIQLLFDDKELDLMMRYADCAVRLQQPQQPDLIQKK
LFTIHMHVYASKNYIANYGKPEKLSELDNHRIISFGEPVPTYLSGLNWLEKAGKSDGALRLSILRINNIISIKNALMRDL
GIAVLPDYIVDNDEHLVRLFPDMVDIPSFDTFFCYPYALKNLAKLKAFRDYIFSKARQWSF
>Mature_300_residues
SSPLDWDKLRVFHAAAEAGSFTHAAQMLHLSQSAISRQVSALEQEVGVSLFQRHARGLILTEQGEILYRTTHDVLMKLES
ARSQLSESCEKPAGNLRVTSTFGMGAGWLVERMPEFLSLYPDMRIQLLFDDKELDLMMRYADCAVRLQQPQQPDLIQKKL
FTIHMHVYASKNYIANYGKPEKLSELDNHRIISFGEPVPTYLSGLNWLEKAGKSDGALRLSILRINNIISIKNALMRDLG
IAVLPDYIVDNDEHLVRLFPDMVDIPSFDTFFCYPYALKNLAKLKAFRDYIFSKARQWSF

Specific function: Unknown

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=292, Percent_Identity=31.5068493150685, Blast_Score=134, Evalue=9e-33,
Organism=Escherichia coli, GI145693193, Length=257, Percent_Identity=27.2373540856031, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1789639, Length=187, Percent_Identity=32.620320855615, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1787589, Length=272, Percent_Identity=28.6764705882353, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI87081978, Length=180, Percent_Identity=30, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787128, Length=195, Percent_Identity=29.2307692307692, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1789173, Length=302, Percent_Identity=26.158940397351, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1789440, Length=190, Percent_Identity=26.8421052631579, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI157672245, Length=258, Percent_Identity=25.5813953488372, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1788626, Length=300, Percent_Identity=27.6666666666667, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1790262, Length=261, Percent_Identity=28.735632183908, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788887, Length=296, Percent_Identity=24.3243243243243, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1786448, Length=141, Percent_Identity=31.2056737588652, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1788706, Length=138, Percent_Identity=30.4347826086957, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787601, Length=133, Percent_Identity=27.8195488721804, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34407; Mature: 34276

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSPLDWDKLRVFHAAAEAGSFTHAAQMLHLSQSAISRQVSALEQEVGVSLFQRHARGLI
CCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEE
LTEQGEILYRTTHDVLMKLESARSQLSESCEKPAGNLRVTSTFGMGAGWLVERMPEFLSL
EECCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHH
YPDMRIQLLFDDKELDLMMRYADCAVRLQQPQQPDLIQKKLFTIHMHVYASKNYIANYGK
CCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHEEEEEEEECCCHHCCCCC
PEKLSELDNHRIISFGEPVPTYLSGLNWLEKAGKSDGALRLSILRINNIISIKNALMRDL
CHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHHHHHHC
GIAVLPDYIVDNDEHLVRLFPDMVDIPSFDTFFCYPYALKNLAKLKAFRDYIFSKARQWS
CHHHCCHHHCCCCHHHHHHHHHHHCCCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHCCC
F
C
>Mature Secondary Structure 
SSPLDWDKLRVFHAAAEAGSFTHAAQMLHLSQSAISRQVSALEQEVGVSLFQRHARGLI
CCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEE
LTEQGEILYRTTHDVLMKLESARSQLSESCEKPAGNLRVTSTFGMGAGWLVERMPEFLSL
EECCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHH
YPDMRIQLLFDDKELDLMMRYADCAVRLQQPQQPDLIQKKLFTIHMHVYASKNYIANYGK
CCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHEEEEEEEECCCHHCCCCC
PEKLSELDNHRIISFGEPVPTYLSGLNWLEKAGKSDGALRLSILRINNIISIKNALMRDL
CHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHHHHHHC
GIAVLPDYIVDNDEHLVRLFPDMVDIPSFDTFFCYPYALKNLAKLKAFRDYIFSKARQWS
CHHHCCHHHCCCCHHHHHHHHHHHCCCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHCCC
F
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]