Click here to switch to the map view.

The map label for this gene is dnaG [H]

Identifier: 49475879

GI number: 49475879

Start: 1275067

End: 1277004

Strand: Reverse

Name: dnaG [H]

Synonym: BH11540

Alternate gene names: 49475879

Gene position: 1277004-1275067 (Counterclockwise)

Preceding gene: 49475889

Following gene: 49475878

Centisome position: 66.13

GC content: 39.78

Gene sequence:

>1938_bases
ATGCGTTTTTCGCCTGATTTTCTTGATGAACTGCGTACCAGATTGCCAATTTCAACAGTTATTGGCCAGAGGGTAACATT
TGATCCTCAAAAAAGTAAACCTTCGCGGGGGGATTTTTGGAGTTGTTGTCCATTTCATGGTGAGAAAACACCAAGCTTTC
ATTGTGATGATCATAAAGGCCGTTATTATTGTTTTGGCTGTGGTGTAAGTGGTGACATTTTTACTTTTCTTTGTGAGCTT
GATGGGTTGCACTTTTCAGAAAGTGTAGTGCGCTTGGCTGATTTTGCTGGAATGAAACTCCCTCTTTTTGATCCACAATC
TCATAAGAGCCAAATGGGAAAGGCTGATCTTTATAATGTTATGAAGATCGCTACGGATTTTTTTCAATATAGTTTACGTG
ATAAAGCAGGTGTGCAGGCGCGTCGTTATCTTGATGCGCGTGGTGTAACGCCAAAGCTTGCAGAGCGCTTTCGGATTGGT
TTTGCTCCGACAAGGCGTACAGCTTTGAAAGAAGCTTTGAATGCGCGTGGTATTTCAATAAAACAAATGGAAGAGTGTGG
GCTTATCAAAATGGGGGAAGATGGTACGACTTCCTCTGACCGATTTAGAAATCGCATTATGTTTCCTATTGAAGATTTAC
GTGGACGTGTTGTGGCCTTTGGAGGGCGTGCGTTAGAAAAAGATATACGGGCAAAGTACCTCAATAGTCCTGAAACAGTG
TTGTTTCATAAAGGAGATATGCTTTACAACGCAGCGTCTGCTCGCAAAAATAGCCGCTTGGTTGGTGATGAAAAAAACCA
TGCACTCCTCGTTGTTGAAGGCTATATGGATGTCATCACATTGACTAAAGCTGGTTTTGAGGGAGCTGTAGCCCCTTTGG
GGACAGCGTTAACAGAAGCGCAAATTGCGCTTTTATGGCAAATAGGAACCGATCCAATTTTGTGTTTTGATGGAGACGAT
GCCGGTTTAAAGGCTGCTTTTCGTGTTGCTGATCGTGTGCTGCCTCTTTTAAAAGCCGGGGTGTCTGTTCGCTTTGTTTT
GTTGCCACAAGGTAAAGATCCAGATGAAATTATTTGCGCTGGTGGTGCAGAGCTTTTTACTTCTTTTTTACAAAAATCAA
TTCCGTTGATTGAACTTTTGTGGTGGCGCGCCACCTATGGAAAGAATTTTGAAATTCCAGAGGCGAGAGCAGCGCTAGAA
AAACAACTCAAACAACAAATTTTTACGATTAAGGATGAAGATATACGACGTTATTATTTGCAAGATATAAAGAAACGACT
TTTTGACTTTTTTCGTCCCTCTTTTTCGAACAAAAAATTTACTGGGCGCTCTTATCAGTCCTCCCCAAATAGGATATTAA
AAGATCAATCGTTTTCTGTTTTGGCAAATTCCAACATCGTACGTAATTCTTCGCACTCTATTCCTCTGCGTGAGGCCGTT
ATTTTATTGATTTTAGCTTATTATCCTGAGTTATGGTATGAAAATTTTGAAGTTCTTGCTGAGTTGGAGTTACAAAACAC
TCAATTAGTGCGTTTTCATCAATCTATGTTGGAAATTCTTGGAAATCAGCAGCTTCATGATAAAGAAACAATGATTGCAC
TTTTAGAAGAAAGAGGGCTAAAAGTTCTTTTAGAGCTCATGAAAAATCTTGTGAAAAAGATTGGTATGCGTACCATCTTT
TATGGTGCTCCCATAGAAGATGCCCGTGCTATATTAAAACAAGCCATCTACTTGCATCTTCAAGAATACCACCTACATAA
GAGACTACAAGATATTGAAGAGCAACTCGTGGAAAATCCTAAAAGTGAGGTGTTTGATCTGCTGTGTGAAACAAAAATAG
AATTGGAACAGATGCAAGCAACAGAAGCTTTAATTGAAGGATTTGGAAGTTGGTTTGATGAAGAAATAGACGGGAATAAG
CAAGATAAGATAAAATGA

Upstream 100 bases:

>100_bases
TGGTCTTTAAAAATTTTCGACCTCTTAAGAGGAAGTCTTGGAATTATGCTCTAAACTCGTTAAATATGCAAAAAGGAATT
TTAAAACTCTGTGAGAGATA

Downstream 100 bases:

>100_bases
TTCGCTTTTTGAGAGCGAATCAGTCACCTAAGGCCAAGTATAAGGAAATTTTATGTAGAGCTACATTTTTATAAAAAATG
ATGACTAAATTTTAGGAATA

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 645; Mature: 645

Protein sequence:

>645_residues
MRFSPDFLDELRTRLPISTVIGQRVTFDPQKSKPSRGDFWSCCPFHGEKTPSFHCDDHKGRYYCFGCGVSGDIFTFLCEL
DGLHFSESVVRLADFAGMKLPLFDPQSHKSQMGKADLYNVMKIATDFFQYSLRDKAGVQARRYLDARGVTPKLAERFRIG
FAPTRRTALKEALNARGISIKQMEECGLIKMGEDGTTSSDRFRNRIMFPIEDLRGRVVAFGGRALEKDIRAKYLNSPETV
LFHKGDMLYNAASARKNSRLVGDEKNHALLVVEGYMDVITLTKAGFEGAVAPLGTALTEAQIALLWQIGTDPILCFDGDD
AGLKAAFRVADRVLPLLKAGVSVRFVLLPQGKDPDEIICAGGAELFTSFLQKSIPLIELLWWRATYGKNFEIPEARAALE
KQLKQQIFTIKDEDIRRYYLQDIKKRLFDFFRPSFSNKKFTGRSYQSSPNRILKDQSFSVLANSNIVRNSSHSIPLREAV
ILLILAYYPELWYENFEVLAELELQNTQLVRFHQSMLEILGNQQLHDKETMIALLEERGLKVLLELMKNLVKKIGMRTIF
YGAPIEDARAILKQAIYLHLQEYHLHKRLQDIEEQLVENPKSEVFDLLCETKIELEQMQATEALIEGFGSWFDEEIDGNK
QDKIK

Sequences:

>Translated_645_residues
MRFSPDFLDELRTRLPISTVIGQRVTFDPQKSKPSRGDFWSCCPFHGEKTPSFHCDDHKGRYYCFGCGVSGDIFTFLCEL
DGLHFSESVVRLADFAGMKLPLFDPQSHKSQMGKADLYNVMKIATDFFQYSLRDKAGVQARRYLDARGVTPKLAERFRIG
FAPTRRTALKEALNARGISIKQMEECGLIKMGEDGTTSSDRFRNRIMFPIEDLRGRVVAFGGRALEKDIRAKYLNSPETV
LFHKGDMLYNAASARKNSRLVGDEKNHALLVVEGYMDVITLTKAGFEGAVAPLGTALTEAQIALLWQIGTDPILCFDGDD
AGLKAAFRVADRVLPLLKAGVSVRFVLLPQGKDPDEIICAGGAELFTSFLQKSIPLIELLWWRATYGKNFEIPEARAALE
KQLKQQIFTIKDEDIRRYYLQDIKKRLFDFFRPSFSNKKFTGRSYQSSPNRILKDQSFSVLANSNIVRNSSHSIPLREAV
ILLILAYYPELWYENFEVLAELELQNTQLVRFHQSMLEILGNQQLHDKETMIALLEERGLKVLLELMKNLVKKIGMRTIF
YGAPIEDARAILKQAIYLHLQEYHLHKRLQDIEEQLVENPKSEVFDLLCETKIELEQMQATEALIEGFGSWFDEEIDGNK
QDKIK
>Mature_645_residues
MRFSPDFLDELRTRLPISTVIGQRVTFDPQKSKPSRGDFWSCCPFHGEKTPSFHCDDHKGRYYCFGCGVSGDIFTFLCEL
DGLHFSESVVRLADFAGMKLPLFDPQSHKSQMGKADLYNVMKIATDFFQYSLRDKAGVQARRYLDARGVTPKLAERFRIG
FAPTRRTALKEALNARGISIKQMEECGLIKMGEDGTTSSDRFRNRIMFPIEDLRGRVVAFGGRALEKDIRAKYLNSPETV
LFHKGDMLYNAASARKNSRLVGDEKNHALLVVEGYMDVITLTKAGFEGAVAPLGTALTEAQIALLWQIGTDPILCFDGDD
AGLKAAFRVADRVLPLLKAGVSVRFVLLPQGKDPDEIICAGGAELFTSFLQKSIPLIELLWWRATYGKNFEIPEARAALE
KQLKQQIFTIKDEDIRRYYLQDIKKRLFDFFRPSFSNKKFTGRSYQSSPNRILKDQSFSVLANSNIVRNSSHSIPLREAV
ILLILAYYPELWYENFEVLAELELQNTQLVRFHQSMLEILGNQQLHDKETMIALLEERGLKVLLELMKNLVKKIGMRTIF
YGAPIEDARAILKQAIYLHLQEYHLHKRLQDIEEQLVENPKSEVFDLLCETKIELEQMQATEALIEGFGSWFDEEIDGNK
QDKIK

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=424, Percent_Identity=37.5, Blast_Score=277, Evalue=1e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013264
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 73578; Mature: 73578

Theoretical pI: Translated: 7.53; Mature: 7.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFSPDFLDELRTRLPISTVIGQRVTFDPQKSKPSRGDFWSCCPFHGEKTPSFHCDDHKG
CCCCHHHHHHHHHHCCHHHHHCCCEECCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCC
RYYCFGCGVSGDIFTFLCELDGLHFSESVVRLADFAGMKLPLFDPQSHKSQMGKADLYNV
CEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHCCHHHHHHH
MKIATDFFQYSLRDKAGVQARRYLDARGVTPKLAERFRIGFAPTRRTALKEALNARGISI
HHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCH
KQMEECGLIKMGEDGTTSSDRFRNRIMFPIEDLRGRVVAFGGRALEKDIRAKYLNSPETV
HHHHHCCCEEECCCCCCCHHHHHCCCCCCHHHHCCCEEEECCHHHHHHHHHHHCCCCCEE
LFHKGDMLYNAASARKNSRLVGDEKNHALLVVEGYMDVITLTKAGFEGAVAPLGTALTEA
EEECCCHHEEHHHHCCCCCCCCCCCCCEEEEEECCHHHHHHHHCCCCCCHHHHHHHHHHH
QIALLWQIGTDPILCFDGDDAGLKAAFRVADRVLPLLKAGVSVRFVLLPQGKDPDEIICA
HEEEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEC
GGAELFTSFLQKSIPLIELLWWRATYGKNFEIPEARAALEKQLKQQIFTIKDEDIRRYYL
CCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHH
QDIKKRLFDFFRPSFSNKKFTGRSYQSSPNRILKDQSFSVLANSNIVRNSSHSIPLREAV
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHCCCCCEEEECCCEEECCCCCCCHHHHH
ILLILAYYPELWYENFEVLAELELQNTQLVRFHQSMLEILGNQQLHDKETMIALLEERGL
HHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCH
KVLLELMKNLVKKIGMRTIFYGAPIEDARAILKQAIYLHLQEYHLHKRLQDIEEQLVENP
HHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KSEVFDLLCETKIELEQMQATEALIEGFGSWFDEEIDGNKQDKIK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MRFSPDFLDELRTRLPISTVIGQRVTFDPQKSKPSRGDFWSCCPFHGEKTPSFHCDDHKG
CCCCHHHHHHHHHHCCHHHHHCCCEECCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCC
RYYCFGCGVSGDIFTFLCELDGLHFSESVVRLADFAGMKLPLFDPQSHKSQMGKADLYNV
CEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHCCHHHHHHH
MKIATDFFQYSLRDKAGVQARRYLDARGVTPKLAERFRIGFAPTRRTALKEALNARGISI
HHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCH
KQMEECGLIKMGEDGTTSSDRFRNRIMFPIEDLRGRVVAFGGRALEKDIRAKYLNSPETV
HHHHHCCCEEECCCCCCCHHHHHCCCCCCHHHHCCCEEEECCHHHHHHHHHHHCCCCCEE
LFHKGDMLYNAASARKNSRLVGDEKNHALLVVEGYMDVITLTKAGFEGAVAPLGTALTEA
EEECCCHHEEHHHHCCCCCCCCCCCCCEEEEEECCHHHHHHHHCCCCCCHHHHHHHHHHH
QIALLWQIGTDPILCFDGDDAGLKAAFRVADRVLPLLKAGVSVRFVLLPQGKDPDEIICA
HEEEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEC
GGAELFTSFLQKSIPLIELLWWRATYGKNFEIPEARAALEKQLKQQIFTIKDEDIRRYYL
CCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHH
QDIKKRLFDFFRPSFSNKKFTGRSYQSSPNRILKDQSFSVLANSNIVRNSSHSIPLREAV
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHCCCCCEEEECCCEEECCCCCCCHHHHH
ILLILAYYPELWYENFEVLAELELQNTQLVRFHQSMLEILGNQQLHDKETMIALLEERGL
HHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCH
KVLLELMKNLVKKIGMRTIFYGAPIEDARAILKQAIYLHLQEYHLHKRLQDIEEQLVENP
HHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KSEVFDLLCETKIELEQMQATEALIEGFGSWFDEEIDGNKQDKIK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]