Click here to switch to the map view.

The map label for this gene is ligA

Identifier: 49475845

GI number: 49475845

Start: 1225998

End: 1228148

Strand: Reverse

Name: ligA

Synonym: BH11140

Alternate gene names: 49475845

Gene position: 1228148-1225998 (Counterclockwise)

Preceding gene: 49475846

Following gene: 49475841

Centisome position: 63.6

GC content: 41.33

Gene sequence:

>2151_bases
ATGCACAAAGACGAAATTCAAAACCTGACTGCTCTTGAAGCAGCAAGTGAATTAGAGTGGTTGGCAAAAGAGATTACTCG
TCATGATGTGCTTTATAATCGTAATGACCAACCTGAAATCTCTGATGCAGAATATGATGCTCTTCGCCGGCGTAATGCGG
AAATTGAAGCTCTCTTTCCTGAACTTATTCGTCCAGATTCTCCCTCACATAAAATTGGAGCACCGATTTCTGAAAAGTTT
GAAAAATCTGTTCATTCCCAGCCGATGCTTTCACTTGATAATGCCTTTAGCTCTGAAGATGTTAGTGAATTTATGGAGCG
TGTTCGGCGTTTTTTACGACTGCCAGAAACACAAATGTTGGAAATAACAGCAGAGCCGAAGATTGATGGTTTATCTTTAT
CTTTGCGCTATGAACAAGGAAGGCTTGTCTGTGCTGCAACGCGTGGTGATGGATATGTCGGTGAAAATGTAACTGCGAAT
GCACGAACAATTTCTGATATTCCGCAAGTTTTACAAGGCAAATTCCCTGATATAATTGAAGTTCGCGGTGAAGTTTATAT
GCGGCGGGCGGATTTCCAAGCACTTAATGTGAATCAACAAGAAAAGGGCAAATTAGCTTTTGCTAATCCTAGAAATGCAG
CGGCTGGTTCTTTACGTCAACTGGATTCAAGAATAACTGCTAGCAGAAAGCTGCAATTTTTTGCTTATGCTTGTGGTGAA
GTGAGTGAAATATTTGCAGAAAGCCAAATGGGGATGATGGAAAAGCTAAAAGAATATGGCTTTGTTATCAATCCATTAAT
TAAAAAAATTAAATCATTAGAAGAGCTTATTTCCTATTATCACGATATCGAAGAACGTCGTCATGCCTTAAGCTATGATA
TTGATGGGATTGTTTATAAGGTTAATGATTTGATGCTGCAAATGCGTTTGGGTTTTGTTTCTCGTTCGCCACGTTGGGCA
ATAGCGCATAAATTTCCAGCAGAAAAAGCTATGACAGTTTTGGAAGGTATTGATATTCAAGTCGGTCGTACGGGAGCATT
AACTCCTGTTGCCCGGCTTGCACCTATTACTGTTGGTGGTGTGGTGATTACCAATGCAAGTTTGCATAATGAGGATTATA
TCAAGGGAATTGGTTCTAAAGGGGAGCCAATTCGTGAGGGAAATGATATTCGTGTAGGCGATACAGTGATTGTACAGCGT
GCTGGTGATGTAATCCCTCAAATTATTGATATTGTTGCTGAAAAGCGTTCAAAGGATGCTTCTGCTTTTGTTTTTCCTTA
TCTGTGTCCAGCTTGTGGAAGTTATGCTGTTCGTGAAATAGGAGAAGCTGTGCGTCGATGCACGGGTGGGCTTATTTGTC
CCGCACAAGCGATTGAGCGTATTCGTCATTTTGTTTCGCGCAATGCCTTTGATATTGAGGGGCTTGGTAAAAAACAGGTG
GAATTTTTTTTTCAGATTCAAGATGAAACGCTTTGTATTCATACGCCAGCTGATATTTTTACGTTACAACGTCGCCAAGA
AAAATCTCTGGTCCATTTGGAAAATATGGAGGGGTTTGGTACTGTTTCAGTGCGTAAACTTTATGATGCTATTAATGCGC
GCCGAAAAATTCCTTTAAGTCGTTTTTTATTTGCACTAGGAATTCGCTATGTTGGAGAGGTTAATGCCCGCCGTCTTGCA
CGTGCTTATCAAAATTATACAGCTTTTGAAACTGCGGCAATGGCAGCTACTATGCCTGACGATAAGGTGGGTAAAGAAGG
CAATGAGGCTTGGATGGAGCTTACCAATATTGAAGGGATTGGACCACAGGTAGGTGAAGCAATTATTGATTTTTATAAGG
AAGTGCATAACCGCGAGGTTTTGTCTGGTTTGCTTTGCGAAGTTACACCTCTGGATGAGGAGTCTGTCATGACTGCTTCT
TCGCCAATAGCAGGAAAGATCATCGTTTTTACAGGAACTTTGACGCGTATGTCACGTGATGAAGCAAAAGCACTTGCTGA
ACGGTTAGGTGCTAAAACGTCTGGTTCTCTTTCTAAAAAAACGAATCTTCTTGTTGCAGGAGTTGGGGGTGGATCGAAAT
TGACCAAAGCACAAGAGTTAGGTGTTGAAGTTATCGATGAAGAGGCCTGGCTGCAGTTGATCGAGGGATAA

Upstream 100 bases:

>100_bases
TATGAATTATATCACTGGCGAGCATGTCTTCATTTGGATTCTGTGCGTGATAAAGAATTGAGATTATTATGTCCGTGGCC
CATAATTTGGAATAAGTTTT

Downstream 100 bases:

>100_bases
TATTTAGGAAAAGGCTTTTTCTAGAATTAGAGTGCCTGTTCGTATCACTATTTCTCTCTTATGGGGCATATTGTTGTGGG
ATTTGTGGGTAGATCGAAAT

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 716; Mature: 716

Protein sequence:

>716_residues
MHKDEIQNLTALEAASELEWLAKEITRHDVLYNRNDQPEISDAEYDALRRRNAEIEALFPELIRPDSPSHKIGAPISEKF
EKSVHSQPMLSLDNAFSSEDVSEFMERVRRFLRLPETQMLEITAEPKIDGLSLSLRYEQGRLVCAATRGDGYVGENVTAN
ARTISDIPQVLQGKFPDIIEVRGEVYMRRADFQALNVNQQEKGKLAFANPRNAAAGSLRQLDSRITASRKLQFFAYACGE
VSEIFAESQMGMMEKLKEYGFVINPLIKKIKSLEELISYYHDIEERRHALSYDIDGIVYKVNDLMLQMRLGFVSRSPRWA
IAHKFPAEKAMTVLEGIDIQVGRTGALTPVARLAPITVGGVVITNASLHNEDYIKGIGSKGEPIREGNDIRVGDTVIVQR
AGDVIPQIIDIVAEKRSKDASAFVFPYLCPACGSYAVREIGEAVRRCTGGLICPAQAIERIRHFVSRNAFDIEGLGKKQV
EFFFQIQDETLCIHTPADIFTLQRRQEKSLVHLENMEGFGTVSVRKLYDAINARRKIPLSRFLFALGIRYVGEVNARRLA
RAYQNYTAFETAAMAATMPDDKVGKEGNEAWMELTNIEGIGPQVGEAIIDFYKEVHNREVLSGLLCEVTPLDEESVMTAS
SPIAGKIIVFTGTLTRMSRDEAKALAERLGAKTSGSLSKKTNLLVAGVGGGSKLTKAQELGVEVIDEEAWLQLIEG

Sequences:

>Translated_716_residues
MHKDEIQNLTALEAASELEWLAKEITRHDVLYNRNDQPEISDAEYDALRRRNAEIEALFPELIRPDSPSHKIGAPISEKF
EKSVHSQPMLSLDNAFSSEDVSEFMERVRRFLRLPETQMLEITAEPKIDGLSLSLRYEQGRLVCAATRGDGYVGENVTAN
ARTISDIPQVLQGKFPDIIEVRGEVYMRRADFQALNVNQQEKGKLAFANPRNAAAGSLRQLDSRITASRKLQFFAYACGE
VSEIFAESQMGMMEKLKEYGFVINPLIKKIKSLEELISYYHDIEERRHALSYDIDGIVYKVNDLMLQMRLGFVSRSPRWA
IAHKFPAEKAMTVLEGIDIQVGRTGALTPVARLAPITVGGVVITNASLHNEDYIKGIGSKGEPIREGNDIRVGDTVIVQR
AGDVIPQIIDIVAEKRSKDASAFVFPYLCPACGSYAVREIGEAVRRCTGGLICPAQAIERIRHFVSRNAFDIEGLGKKQV
EFFFQIQDETLCIHTPADIFTLQRRQEKSLVHLENMEGFGTVSVRKLYDAINARRKIPLSRFLFALGIRYVGEVNARRLA
RAYQNYTAFETAAMAATMPDDKVGKEGNEAWMELTNIEGIGPQVGEAIIDFYKEVHNREVLSGLLCEVTPLDEESVMTAS
SPIAGKIIVFTGTLTRMSRDEAKALAERLGAKTSGSLSKKTNLLVAGVGGGSKLTKAQELGVEVIDEEAWLQLIEG
>Mature_716_residues
MHKDEIQNLTALEAASELEWLAKEITRHDVLYNRNDQPEISDAEYDALRRRNAEIEALFPELIRPDSPSHKIGAPISEKF
EKSVHSQPMLSLDNAFSSEDVSEFMERVRRFLRLPETQMLEITAEPKIDGLSLSLRYEQGRLVCAATRGDGYVGENVTAN
ARTISDIPQVLQGKFPDIIEVRGEVYMRRADFQALNVNQQEKGKLAFANPRNAAAGSLRQLDSRITASRKLQFFAYACGE
VSEIFAESQMGMMEKLKEYGFVINPLIKKIKSLEELISYYHDIEERRHALSYDIDGIVYKVNDLMLQMRLGFVSRSPRWA
IAHKFPAEKAMTVLEGIDIQVGRTGALTPVARLAPITVGGVVITNASLHNEDYIKGIGSKGEPIREGNDIRVGDTVIVQR
AGDVIPQIIDIVAEKRSKDASAFVFPYLCPACGSYAVREIGEAVRRCTGGLICPAQAIERIRHFVSRNAFDIEGLGKKQV
EFFFQIQDETLCIHTPADIFTLQRRQEKSLVHLENMEGFGTVSVRKLYDAINARRKIPLSRFLFALGIRYVGEVNARRLA
RAYQNYTAFETAAMAATMPDDKVGKEGNEAWMELTNIEGIGPQVGEAIIDFYKEVHNREVLSGLLCEVTPLDEESVMTAS
SPIAGKIIVFTGTLTRMSRDEAKALAERLGAKTSGSLSKKTNLLVAGVGGGSKLTKAQELGVEVIDEEAWLQLIEG

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Homo sapiens, GI32528306, Length=104, Percent_Identity=43.2692307692308, Blast_Score=70, Evalue=1e-11,
Organism=Escherichia coli, GI1788750, Length=704, Percent_Identity=44.6022727272727, Blast_Score=574, Evalue=1e-165,
Organism=Escherichia coli, GI87082305, Length=545, Percent_Identity=22.3853211009174, Blast_Score=99, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_BARHE (Q6G2R5)

Other databases:

- EMBL:   BX897699
- RefSeq:   YP_033886.1
- HSSP:   P26996
- ProteinModelPortal:   Q6G2R5
- GeneID:   2865583
- GenomeReviews:   BX897699_GR
- KEGG:   bhe:BH11140
- NMPDR:   fig|283166.1.peg.1014
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- PhylomeDB:   Q6G2R5
- ProtClustDB:   PRK07956
- BioCyc:   BHEN283166:BH11140-MONOMER
- BRENDA:   6.5.1.2
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 79737; Mature: 79737

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 127-127 BINDING 125-125 BINDING 148-148 BINDING 184-184 BINDING 300-300 BINDING 324-324

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKDEIQNLTALEAASELEWLAKEITRHDVLYNRNDQPEISDAEYDALRRRNAEIEALFP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCHHHHHHH
ELIRPDSPSHKIGAPISEKFEKSVHSQPMLSLDNAFSSEDVSEFMERVRRFLRLPETQML
HHHCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEE
EITAEPKIDGLSLSLRYEQGRLVCAATRGDGYVGENVTANARTISDIPQVLQGKFPDIIE
EEECCCCCCCEEEEEEECCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHH
VRGEVYMRRADFQALNVNQQEKGKLAFANPRNAAAGSLRQLDSRITASRKLQFFAYACGE
HHHHHHEECCCCEEECCCCHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSEIFAESQMGMMEKLKEYGFVINPLIKKIKSLEELISYYHDIEERRHALSYDIDGIVYK
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEH
VNDLMLQMRLGFVSRSPRWAIAHKFPAEKAMTVLEGIDIQVGRTGALTPVARLAPITVGG
HHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHCCCEEECCCCCCCHHHHHCCEEECC
VVITNASLHNEDYIKGIGSKGEPIREGNDIRVGDTVIVQRAGDVIPQIIDIVAEKRSKDA
EEEECCCCCCCHHHHCCCCCCCCCCCCCCEEECCCEEEEHHHHHHHHHHHHHHHHCCCCC
SAFVFPYLCPACGSYAVREIGEAVRRCTGGLICPAQAIERIRHFVSRNAFDIEGLGKKQV
CEEEHHHHHHHHCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHE
EFFFQIQDETLCIHTPADIFTLQRRQEKSLVHLENMEGFGTVSVRKLYDAINARRKIPLS
EEEEEECCCEEEEECCHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCHH
RFLFALGIRYVGEVNARRLARAYQNYTAFETAAMAATMPDDKVGKEGNEAWMELTNIEGI
HHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHCCCCCHHCCCCCCHHHHEECCCCCC
GPQVGEAIIDFYKEVHNREVLSGLLCEVTPLDEESVMTASSPIAGKIIVFTGTLTRMSRD
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCEECCCCCCCEEEEEECCHHHHHHH
EAKALAERLGAKTSGSLSKKTNLLVAGVGGGSKLTKAQELGVEVIDEEAWLQLIEG
HHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCHHHHHHHCCCCEECHHHHHHHHCC
>Mature Secondary Structure
MHKDEIQNLTALEAASELEWLAKEITRHDVLYNRNDQPEISDAEYDALRRRNAEIEALFP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCHHHHHHH
ELIRPDSPSHKIGAPISEKFEKSVHSQPMLSLDNAFSSEDVSEFMERVRRFLRLPETQML
HHHCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEE
EITAEPKIDGLSLSLRYEQGRLVCAATRGDGYVGENVTANARTISDIPQVLQGKFPDIIE
EEECCCCCCCEEEEEEECCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHH
VRGEVYMRRADFQALNVNQQEKGKLAFANPRNAAAGSLRQLDSRITASRKLQFFAYACGE
HHHHHHEECCCCEEECCCCHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSEIFAESQMGMMEKLKEYGFVINPLIKKIKSLEELISYYHDIEERRHALSYDIDGIVYK
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEH
VNDLMLQMRLGFVSRSPRWAIAHKFPAEKAMTVLEGIDIQVGRTGALTPVARLAPITVGG
HHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHCCCEEECCCCCCCHHHHHCCEEECC
VVITNASLHNEDYIKGIGSKGEPIREGNDIRVGDTVIVQRAGDVIPQIIDIVAEKRSKDA
EEEECCCCCCCHHHHCCCCCCCCCCCCCCEEECCCEEEEHHHHHHHHHHHHHHHHCCCCC
SAFVFPYLCPACGSYAVREIGEAVRRCTGGLICPAQAIERIRHFVSRNAFDIEGLGKKQV
CEEEHHHHHHHHCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHE
EFFFQIQDETLCIHTPADIFTLQRRQEKSLVHLENMEGFGTVSVRKLYDAINARRKIPLS
EEEEEECCCEEEEECCHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCHH
RFLFALGIRYVGEVNARRLARAYQNYTAFETAAMAATMPDDKVGKEGNEAWMELTNIEGI
HHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHCCCCCHHCCCCCCHHHHEECCCCCC
GPQVGEAIIDFYKEVHNREVLSGLLCEVTPLDEESVMTASSPIAGKIIVFTGTLTRMSRD
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCEECCCCCCCEEEEEECCHHHHHHH
EAKALAERLGAKTSGSLSKKTNLLVAGVGGGSKLTKAQELGVEVIDEEAWLQLIEG
HHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCHHHHHHHCCCCEECHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA