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The map label for this gene is thyA

Identifier: 49475832

GI number: 49475832

Start: 1208967

End: 1209761

Strand: Reverse

Name: thyA

Synonym: BH10980

Alternate gene names: 49475832

Gene position: 1209761-1208967 (Counterclockwise)

Preceding gene: 49475836

Following gene: 49475831

Centisome position: 62.65

GC content: 37.48

Gene sequence:

>795_bases
ATGAAAACCTATCTTGATCTTTTATCTCATGTTTTAAATAAAGGCATTGATCGTACTGACAGAACTGGTGTAGGAACACG
ATCGGTTTTTGGCTATCAGATGCGGTTTGATTTACAGGCTGGATTTCCATTGTTAACAACGAAGAAATTGCATTTACGCT
CGATCATTTATGAGCTTTTGTGGTTTCTTAGAGGTGATACCAATATTGCCTGGTTGAAAGAACATGGTGTGTCAATTTGG
GATGAGTGGGCTGATGAAAAAGGCAATCTTGGTCCTATTTATGGTTATCAGTGGCGTTCTTGGCCAGCTCCTGATGGACG
TCATATTGATCAGATCAGTAATCTTTTGATGATGATTAAAAAAACGCCTGATTCTCGTCGTTTAATTGTCTCTGCGTGGA
ATCCTGCCTCGATAAAGGAAATGGCTCTTCCACCATGCCATTGTTTTTTTCAATTTTATGTTGCAGATGGTAAATTGTCT
TGCCAACTTTATCAACGTTCTGCAGATATTTTTTTAGGTATTCCGTTCAATATTGCTTCCTATGCGTTATTAACAATGAT
GATTGCACAAGTAAGTGGTCTTAAAGTCGGCGATTTTATTCATACTCTTGGTGATGCGCATCTTTATTCTAATCATTTTG
AACAGGCTCAACATCAATTGTCTCGTATACCAAATGCTTTGCCGTTTATGCGTATAAATCCAGCGGTAACAGATCTTTTT
TCTTTTAAATTTGAGGATTTTGAATTGCTTAATTATGATGCACAACCACATATCAAGGCGCCAATAGCAGTATGA

Upstream 100 bases:

>100_bases
TTCTTGTGCAAGAAATTTGAGGAAAGAGATTTAAACTATATTTTATAAATTTTACAGTTTCTATTTTCTATAATCAATAG
AATCGGTCATACTGCTTTAT

Downstream 100 bases:

>100_bases
CTTTTCCCATTTGTTTAATTGCAGCTGTTGCAAAAAATGGTGTTATCGGTCGTGAAGGTGCGATGCCTTGGCATTTATCG
ACGGATTTGCAACGCTTTAA

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MKTYLDLLSHVLNKGIDRTDRTGVGTRSVFGYQMRFDLQAGFPLLTTKKLHLRSIIYELLWFLRGDTNIAWLKEHGVSIW
DEWADEKGNLGPIYGYQWRSWPAPDGRHIDQISNLLMMIKKTPDSRRLIVSAWNPASIKEMALPPCHCFFQFYVADGKLS
CQLYQRSADIFLGIPFNIASYALLTMMIAQVSGLKVGDFIHTLGDAHLYSNHFEQAQHQLSRIPNALPFMRINPAVTDLF
SFKFEDFELLNYDAQPHIKAPIAV

Sequences:

>Translated_264_residues
MKTYLDLLSHVLNKGIDRTDRTGVGTRSVFGYQMRFDLQAGFPLLTTKKLHLRSIIYELLWFLRGDTNIAWLKEHGVSIW
DEWADEKGNLGPIYGYQWRSWPAPDGRHIDQISNLLMMIKKTPDSRRLIVSAWNPASIKEMALPPCHCFFQFYVADGKLS
CQLYQRSADIFLGIPFNIASYALLTMMIAQVSGLKVGDFIHTLGDAHLYSNHFEQAQHQLSRIPNALPFMRINPAVTDLF
SFKFEDFELLNYDAQPHIKAPIAV
>Mature_264_residues
MKTYLDLLSHVLNKGIDRTDRTGVGTRSVFGYQMRFDLQAGFPLLTTKKLHLRSIIYELLWFLRGDTNIAWLKEHGVSIW
DEWADEKGNLGPIYGYQWRSWPAPDGRHIDQISNLLMMIKKTPDSRRLIVSAWNPASIKEMALPPCHCFFQFYVADGKLS
CQLYQRSADIFLGIPFNIASYALLTMMIAQVSGLKVGDFIHTLGDAHLYSNHFEQAQHQLSRIPNALPFMRINPAVTDLF
SFKFEDFELLNYDAQPHIKAPIAV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily

Homologues:

Organism=Homo sapiens, GI4507751, Length=281, Percent_Identity=49.1103202846975, Blast_Score=277, Evalue=6e-75,
Organism=Escherichia coli, GI1789191, Length=264, Percent_Identity=64.7727272727273, Blast_Score=373, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI71993377, Length=282, Percent_Identity=45.7446808510638, Blast_Score=259, Evalue=1e-69,
Organism=Saccharomyces cerevisiae, GI6324648, Length=230, Percent_Identity=45.2173913043478, Blast_Score=197, Evalue=2e-51,
Organism=Drosophila melanogaster, GI17137556, Length=283, Percent_Identity=50.8833922261484, Blast_Score=290, Evalue=7e-79,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): TYSY_BARHE (Q6G2S8)

Other databases:

- EMBL:   BX897699
- RefSeq:   YP_033873.1
- ProteinModelPortal:   Q6G2S8
- SMR:   Q6G2S8
- GeneID:   2865223
- GenomeReviews:   BX897699_GR
- KEGG:   bhe:BH10980
- NMPDR:   fig|283166.1.peg.1001
- HOGENOM:   HBG588098
- OMA:   RMALAPC
- PhylomeDB:   Q6G2S8
- ProtClustDB:   PRK01827
- BioCyc:   BHEN283166:BH10980-MONOMER
- BRENDA:   2.1.1.45
- GO:   GO:0005737
- HAMAP:   MF_00008
- InterPro:   IPR000398
- InterPro:   IPR020940
- Gene3D:   G3DSA:3.30.572.10
- PANTHER:   PTHR11549:SF2
- PRINTS:   PR00108
- TIGRFAMs:   TIGR03284

Pfam domain/function: PF00303 Thymidylat_synt; SSF55831 Thymidylat_synth_C

EC number: =2.1.1.45

Molecular weight: Translated: 30294; Mature: 30294

Theoretical pI: Translated: 8.11; Mature: 8.11

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: ACT_SITE 146-146

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTYLDLLSHVLNKGIDRTDRTGVGTRSVFGYQMRFDLQAGFPLLTTKKLHLRSIIYELL
CCHHHHHHHHHHHCCCCCCCCCCCCCHHEEEEEEEEEECCCCCEEECHHHHHHHHHHHHH
WFLRGDTNIAWLKEHGVSIWDEWADEKGNLGPIYGYQWRSWPAPDGRHIDQISNLLMMIK
HHHCCCCCEEEEHHCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
KTPDSRRLIVSAWNPASIKEMALPPCHCFFQFYVADGKLSCQLYQRSADIFLGIPFNIAS
CCCCCCEEEEEECCCCCHHHHCCCHHHHEEEEEEECCEEEEEEEECCCCEEEECCHHHHH
YALLTMMIAQVSGLKVGDFIHTLGDAHLYSNHFEQAQHQLSRIPNALPFMRINPAVTDLF
HHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCEEECHHHHHHH
SFKFEDFELLNYDAQPHIKAPIAV
HHCCCCEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MKTYLDLLSHVLNKGIDRTDRTGVGTRSVFGYQMRFDLQAGFPLLTTKKLHLRSIIYELL
CCHHHHHHHHHHHCCCCCCCCCCCCCHHEEEEEEEEEECCCCCEEECHHHHHHHHHHHHH
WFLRGDTNIAWLKEHGVSIWDEWADEKGNLGPIYGYQWRSWPAPDGRHIDQISNLLMMIK
HHHCCCCCEEEEHHCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
KTPDSRRLIVSAWNPASIKEMALPPCHCFFQFYVADGKLSCQLYQRSADIFLGIPFNIAS
CCCCCCEEEEEECCCCCHHHHCCCHHHHEEEEEEECCEEEEEEEECCCCEEEECCHHHHH
YALLTMMIAQVSGLKVGDFIHTLGDAHLYSNHFEQAQHQLSRIPNALPFMRINPAVTDLF
HHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCEEECHHHHHHH
SFKFEDFELLNYDAQPHIKAPIAV
HHCCCCEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA