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The map label for this gene is folA [H]

Identifier: 49475831

GI number: 49475831

Start: 1208461

End: 1208970

Strand: Reverse

Name: folA [H]

Synonym: BH10970

Alternate gene names: 49475831

Gene position: 1208970-1208461 (Counterclockwise)

Preceding gene: 49475832

Following gene: 49475830

Centisome position: 62.61

GC content: 38.82

Gene sequence:

>510_bases
ATGACTTTTCCCATTTGTTTAATTGCAGCTGTTGCAAAAAATGGTGTTATCGGTCGTGAAGGTGCGATGCCTTGGCATTT
ATCGACGGATTTGCAACGCTTTAAGGCTTTAACTTTCGGTAGTCCTGTGATTATGGGGAGAAAGACTTGGGATTCTCTCA
GGGCTCCTTTACCAGGGCGTACAAATATTGTCATTACGCGCAATTGTGCATTCAAGGCTGAAAGCGCTGTTATTACCCAC
TCTTTGTCTCATGCTTGTTCTATCGCAAAAAAGGTGGCTTCTCAAAATGGTGCAGATGCAATTTTTATTATTGGTGGTGG
GGAAATTTTTCAACAATGTTTTGATATTGCTGATAAAATATTCCTTACAGAAGTTTTAGATTCCATCGAAGGTGATAGTT
TTTTTCCTGTTTTTGATAAAGAGAAGTGGACTATTGTGCAAACACAATATATTCCAGAAGGAGATAAAGACAGTCATCCG
ACACGTTTTGTGGTTTATGAGCGAAAATAA

Upstream 100 bases:

>100_bases
GTATAAATCCAGCGGTAACAGATCTTTTTTCTTTTAAATTTGAGGATTTTGAATTGCTTAATTATGATGCACAACCACAT
ATCAAGGCGCCAATAGCAGT

Downstream 100 bases:

>100_bases
TTATTTGTAATTAAGTGAAAAGAGGCTATCAGTTGCCTTGTAATGAGATGAGAGGCACCCTATAGATCAAGTGAATGGTT
GGTATTTTAGGAATGGTAGT

Product: dihydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 169; Mature: 168

Protein sequence:

>169_residues
MTFPICLIAAVAKNGVIGREGAMPWHLSTDLQRFKALTFGSPVIMGRKTWDSLRAPLPGRTNIVITRNCAFKAESAVITH
SLSHACSIAKKVASQNGADAIFIIGGGEIFQQCFDIADKIFLTEVLDSIEGDSFFPVFDKEKWTIVQTQYIPEGDKDSHP
TRFVVYERK

Sequences:

>Translated_169_residues
MTFPICLIAAVAKNGVIGREGAMPWHLSTDLQRFKALTFGSPVIMGRKTWDSLRAPLPGRTNIVITRNCAFKAESAVITH
SLSHACSIAKKVASQNGADAIFIIGGGEIFQQCFDIADKIFLTEVLDSIEGDSFFPVFDKEKWTIVQTQYIPEGDKDSHP
TRFVVYERK
>Mature_168_residues
TFPICLIAAVAKNGVIGREGAMPWHLSTDLQRFKALTFGSPVIMGRKTWDSLRAPLPGRTNIVITRNCAFKAESAVITHS
LSHACSIAKKVASQNGADAIFIIGGGEIFQQCFDIADKIFLTEVLDSIEGDSFFPVFDKEKWTIVQTQYIPEGDKDSHPT
RFVVYERK

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Homo sapiens, GI4503323, Length=153, Percent_Identity=31.3725490196078, Blast_Score=84, Evalue=5e-17,
Organism=Homo sapiens, GI28827793, Length=153, Percent_Identity=30.718954248366, Blast_Score=74, Evalue=4e-14,
Organism=Escherichia coli, GI1786233, Length=165, Percent_Identity=36.3636363636364, Blast_Score=117, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17505899, Length=157, Percent_Identity=31.8471337579618, Blast_Score=83, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6324810, Length=136, Percent_Identity=30.1470588235294, Blast_Score=72, Evalue=6e-14,
Organism=Drosophila melanogaster, GI116008026, Length=171, Percent_Identity=25.1461988304094, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24647458, Length=171, Percent_Identity=25.1461988304094, Blast_Score=64, Evalue=5e-11,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 18723; Mature: 18592

Theoretical pI: Translated: 7.97; Mature: 7.97

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFPICLIAAVAKNGVIGREGAMPWHLSTDLQRFKALTFGSPVIMGRKTWDSLRAPLPGR
CCCHHHHHHHHHHCCCCCCCCCCCEECHHHHHHHHHHHCCCCEEECCCCHHHHCCCCCCC
TNIVITRNCAFKAESAVITHSLSHACSIAKKVASQNGADAIFIIGGGEIFQQCFDIADKI
CEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHHH
FLTEVLDSIEGDSFFPVFDKEKWTIVQTQYIPEGDKDSHPTRFVVYERK
HHHHHHHHCCCCCCCEEECCCCEEEEEEEECCCCCCCCCCCEEEEEECC
>Mature Secondary Structure 
TFPICLIAAVAKNGVIGREGAMPWHLSTDLQRFKALTFGSPVIMGRKTWDSLRAPLPGR
CCHHHHHHHHHHCCCCCCCCCCCEECHHHHHHHHHHHCCCCEEECCCCHHHHCCCCCCC
TNIVITRNCAFKAESAVITHSLSHACSIAKKVASQNGADAIFIIGGGEIFQQCFDIADKI
CEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHHH
FLTEVLDSIEGDSFFPVFDKEKWTIVQTQYIPEGDKDSHPTRFVVYERK
HHHHHHHHCCCCCCCEEECCCCEEEEEEEECCCCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]