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The map label for this gene is tgt [H]

Identifier: 49475752

GI number: 49475752

Start: 1116640

End: 1117758

Strand: Reverse

Name: tgt [H]

Synonym: BH10080

Alternate gene names: 49475752

Gene position: 1117758-1116640 (Counterclockwise)

Preceding gene: 49475753

Following gene: 49475751

Centisome position: 57.88

GC content: 42.27

Gene sequence:

>1119_bases
ATGATAAAGACATTTCATTATAGAAAAATTGCTCAAGATGGGTCTGCACGTCGAGGCGAAATTATAACGGGACGTGGGTG
TATTCGTACACCGGCATTTATGCCTGTTGGAACGGCTGGAACCGTTAAAGCTATGTATATGGATCAAATACGTGCTCTTG
GAGCAGATATCATTTTGGGTAATACTTATCATTTAATGCTGCGTCCAGGAGCTGAGTGTGTTGCGCGTTTAGGGGGCTTA
CATCATTTTTCACGTTGGCATGGACCTATTTTAACGGATTCTGGTGGATTTCAGGTTATGTCGCTGGCGGGAATCCGTAA
AATTACCGAACAAAGCGTGATTTTTCGCTCTTATATTAATGGAGCGTATTATGAAATGAGTCCAGAGCGTTCTATTGAGA
TTCAAGGGCTTCTTAACTCTGATATACAGATGCAGCTAGATCAATGTATTGCATTACCTGCAAGTGAAAAAGAAGTAGAA
TCTGCTATGGAACTTTCATTGCGGTGGGCGCAGCGTTGTAAAACAGCTTTTGGTGATCAGCCAGATAAAGCATTGTTTGG
TATTGTTCAAGGCGGTGATAATATGAAAATGCGTGAACGGTCTGCCCAAGCATTGAAAGAGATGGGTTTAAAAGGCTATG
CTATTGGAGGGTTGGCAGTTGGTGAACCGCAGAATGTTATGACAGCTGTATTGGATGTTACTTGTCCAATTTTGCCAGAG
GATAAGCCACGTTATCTCATGGGGGTTGGTACACCTGATGATATTTTACAAAGTGTTGCTCGTGGTATTGATATGTTTGA
TTGTGTTATGCCGACACGTGCAGGGCGCCATGGTTTAGCTTTTACGCGCTTTGGGAGAATTAATTTGCGCAATGCGCGTT
ATGCAGAGGACCCACATCCTCTTGATCCGCAGTCACTTTGTCCTGCGGCAAATGATTATAGTTGTGCTTATTTGCATCAT
TTGATTAGATCTGGCGAATCTCTTGGTGGTATGTTGTTAACTTGGAATAATCTTTTTTACTATCAGCAGCTTATGCAAGG
AATTCGTGATGCCATTGAAGAAGGATGTTATGCTGATTTTTGTGCTGAAACCCGTGCTTTATGGACGAAAGGACGATAA

Upstream 100 bases:

>100_bases
AAAGAATCAATCCATAAAGTTTATGCTGTTGCTGGTAAGAGAGGTATAGCTTTTCTTTCTTATAACAATATGGATTTATG
GAAGCGAAAAGACGAGAGTG

Downstream 100 bases:

>100_bases
GACGTTGATCCTAATGCATGGCGTCTGTTTTTTTCATGTTGTTTTTTTATTTTTGCATTAAAGAGTTTTGCTAAAATTAT
GAAGTGGGAAATGCTTTTCA

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MIKTFHYRKIAQDGSARRGEIITGRGCIRTPAFMPVGTAGTVKAMYMDQIRALGADIILGNTYHLMLRPGAECVARLGGL
HHFSRWHGPILTDSGGFQVMSLAGIRKITEQSVIFRSYINGAYYEMSPERSIEIQGLLNSDIQMQLDQCIALPASEKEVE
SAMELSLRWAQRCKTAFGDQPDKALFGIVQGGDNMKMRERSAQALKEMGLKGYAIGGLAVGEPQNVMTAVLDVTCPILPE
DKPRYLMGVGTPDDILQSVARGIDMFDCVMPTRAGRHGLAFTRFGRINLRNARYAEDPHPLDPQSLCPAANDYSCAYLHH
LIRSGESLGGMLLTWNNLFYYQQLMQGIRDAIEEGCYADFCAETRALWTKGR

Sequences:

>Translated_372_residues
MIKTFHYRKIAQDGSARRGEIITGRGCIRTPAFMPVGTAGTVKAMYMDQIRALGADIILGNTYHLMLRPGAECVARLGGL
HHFSRWHGPILTDSGGFQVMSLAGIRKITEQSVIFRSYINGAYYEMSPERSIEIQGLLNSDIQMQLDQCIALPASEKEVE
SAMELSLRWAQRCKTAFGDQPDKALFGIVQGGDNMKMRERSAQALKEMGLKGYAIGGLAVGEPQNVMTAVLDVTCPILPE
DKPRYLMGVGTPDDILQSVARGIDMFDCVMPTRAGRHGLAFTRFGRINLRNARYAEDPHPLDPQSLCPAANDYSCAYLHH
LIRSGESLGGMLLTWNNLFYYQQLMQGIRDAIEEGCYADFCAETRALWTKGR
>Mature_372_residues
MIKTFHYRKIAQDGSARRGEIITGRGCIRTPAFMPVGTAGTVKAMYMDQIRALGADIILGNTYHLMLRPGAECVARLGGL
HHFSRWHGPILTDSGGFQVMSLAGIRKITEQSVIFRSYINGAYYEMSPERSIEIQGLLNSDIQMQLDQCIALPASEKEVE
SAMELSLRWAQRCKTAFGDQPDKALFGIVQGGDNMKMRERSAQALKEMGLKGYAIGGLAVGEPQNVMTAVLDVTCPILPE
DKPRYLMGVGTPDDILQSVARGIDMFDCVMPTRAGRHGLAFTRFGRINLRNARYAEDPHPLDPQSLCPAANDYSCAYLHH
LIRSGESLGGMLLTWNNLFYYQQLMQGIRDAIEEGCYADFCAETRALWTKGR

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=346, Percent_Identity=43.6416184971098, Blast_Score=287, Evalue=9e-78,
Organism=Homo sapiens, GI13375872, Length=278, Percent_Identity=26.2589928057554, Blast_Score=93, Evalue=4e-19,
Organism=Escherichia coli, GI1786607, Length=352, Percent_Identity=53.9772727272727, Blast_Score=374, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17544680, Length=358, Percent_Identity=40.5027932960894, Blast_Score=263, Evalue=2e-70,
Organism=Drosophila melanogaster, GI19920510, Length=367, Percent_Identity=43.0517711171662, Blast_Score=298, Evalue=4e-81,
Organism=Drosophila melanogaster, GI21355311, Length=247, Percent_Identity=26.3157894736842, Blast_Score=76, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 41254; Mature: 41254

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
7.5 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
7.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKTFHYRKIAQDGSARRGEIITGRGCIRTPAFMPVGTAGTVKAMYMDQIRALGADIILG
CCCCCHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEC
NTYHLMLRPGAECVARLGGLHHFSRWHGPILTDSGGFQVMSLAGIRKITEQSVIFRSYIN
CEEEEEECCCHHHHHHHHHHHHHHHHCCCEEECCCCEEEHHHHHHHHHHHHHHHHHHHHC
GAYYEMSPERSIEIQGLLNSDIQMQLDQCIALPASEKEVESAMELSLRWAQRCKTAFGDQ
CCEEEECCCCCEEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
PDKALFGIVQGGDNMKMRERSAQALKEMGLKGYAIGGLAVGEPQNVMTAVLDVTCPILPE
CCHHEEEEEECCCCCHHHHHHHHHHHHCCCCCEEECCEECCCCHHHHHHHHHHHCCCCCC
DKPRYLMGVGTPDDILQSVARGIDMFDCVMPTRAGRHGLAFTRFGRINLRNARYAEDPHP
CCCCEEEECCCHHHHHHHHHCCCCHHHHHCCCCCCCCCCEEEEECCEEECCCCCCCCCCC
LDPQSLCPAANDYSCAYLHHLIRSGESLGGMLLTWNNLFYYQQLMQGIRDAIEEGCYADF
CCHHHHCCCCCCCHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCHHHH
CAETRALWTKGR
HHHHHHHHCCCC
>Mature Secondary Structure
MIKTFHYRKIAQDGSARRGEIITGRGCIRTPAFMPVGTAGTVKAMYMDQIRALGADIILG
CCCCCHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEC
NTYHLMLRPGAECVARLGGLHHFSRWHGPILTDSGGFQVMSLAGIRKITEQSVIFRSYIN
CEEEEEECCCHHHHHHHHHHHHHHHHCCCEEECCCCEEEHHHHHHHHHHHHHHHHHHHHC
GAYYEMSPERSIEIQGLLNSDIQMQLDQCIALPASEKEVESAMELSLRWAQRCKTAFGDQ
CCEEEECCCCCEEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
PDKALFGIVQGGDNMKMRERSAQALKEMGLKGYAIGGLAVGEPQNVMTAVLDVTCPILPE
CCHHEEEEEECCCCCHHHHHHHHHHHHCCCCCEEECCEECCCCHHHHHHHHHHHCCCCCC
DKPRYLMGVGTPDDILQSVARGIDMFDCVMPTRAGRHGLAFTRFGRINLRNARYAEDPHP
CCCCEEEECCCHHHHHHHHHCCCCHHHHHCCCCCCCCCCEEEEECCEEECCCCCCCCCCC
LDPQSLCPAANDYSCAYLHHLIRSGESLGGMLLTWNNLFYYQQLMQGIRDAIEEGCYADF
CCHHHHCCCCCCCHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCHHHH
CAETRALWTKGR
HHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]