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The map label for this gene is aspS [H]

Identifier: 49475729

GI number: 49475729

Start: 1074730

End: 1076520

Strand: Reverse

Name: aspS [H]

Synonym: BH09790

Alternate gene names: 49475729

Gene position: 1076520-1074730 (Counterclockwise)

Preceding gene: 49475731

Following gene: 49475728

Centisome position: 55.75

GC content: 41.71

Gene sequence:

>1791_bases
ATGCACCGTTATCGCAGTCATCATTGCGCTGCTCTTCGTAAATGTGATGTAGGAATAAAAGCGCGTCTTTCGGGATGGGT
TCATCGTGTTCGTGATCATGGCGGAATCCTTTTTGTAGATTTGCGTGATCATTTTGGAATCACACAAATTGTTGCTGATC
CTGCTTCGCCAGCTTTTAAAATTATTGAAAAAGTGCGTTCTGAATGGGTCATTCGTGTAGATGGGGAAGTGCGCGCGCGT
TCTGATGAAGTTATTAATACATCTCTTCCAACAGGTGAGATTGAGATTTTTGCAAAGGAAGTGGAAATTCTTTCAAAATC
TGATGAGCTTCCTTTACCGGTTTTTGGCGAACCTGATTATCCAGAAGATATTCGATTGAAGTATCGTTTTCTTGATTTAC
GTCGCGAAACTATGCACAGGAATATTATGCGTCGTACTGAGATTATTGCTGCTATCAGACGTTCTATGCAAGAAAATGGT
TTTACAGAATTTACGACACCGCTTTTGACAGCCTCATCACCAGAAGGGGCACGCGATTTTTTGGTTCCAAGTCGTATTCA
TCAGGGAAAATTTTATGCATTGCCACAAGCACCTCAGCAATACAAACAGTTGTTGATGATGTCAGGTTTTGATCGTTATT
TTCAAATTGCTCCCTGTTTTAGAGATGAAGATCCGCGGGCTGATCGTCTTCCAGGCGAGTTTTATCAATTAGATGTTGAA
ATGAGTTTTGTTGAACAGGAAGATGTTTTGGTAACAATGGAACCGATTATGCGTTCTCTTTTTGAAGAGTTTGCAGATGG
AAAACCTGTAACGCAAAGCTTTCCTCGTCTTTCCTATGAGGAGGCAATGCGAAAATATGGCTCGGATAAGCCTGATTTAC
GTAATCCTATCATTATGGAAGATGTCTCTCAGCATTTTTATGATTCTGGTTTTAAAGTTTTTGCTCAAATTTTAGCAAAT
GATGAGAATGCACGGGTGTGGGCTATTCCAGCTAAAACGGGTGGAAGTCGTGCTTTTTGTGATCGTATGAATGGATGGGC
ACAAAGTGAAGGACAACCAGGACTCGGTTATATTTTCTGGCGTGAAGAACAGGGAAAGTTTGAAGGAGCGGGCCCTATCG
CTAAAAATATCGGTGAACAGAGGACTGAAGCACTTCGTATGCAGCTTGGACTTGAAAATGGTGATGCCTGCTTTTTTGTT
GCGGGAGATCCAAAGAAATTTTTACCTTTTGCCGGAGCTGCGCGTACTCGGGTAGGGGAAGAATTAGACCTTGTTGATCG
AGACTGTTTTTCTTTAGCGTGGATTGTTGATTTTCCGTTTTTTGAATGGAATGAAGAGGAGAAAAAGCTTGATTTTGCAC
ATAATCCTTTTTCAATGCCTCAAGGTGGAAAAGATGCTCTTGAATGTCAAGATCCTCTTACTCTTAAGGCTTTTCAATAT
GATCTTGTTTGCAATGGTTATGAGATTGCCTCGGGTGGAATCCGTAATCACTCTCCAGAAATGATGCTTAAGGTTTTTAA
TCTTGCTGGTCTTTCTAAAGAAGTGGTAGAAGATCGTTTTGGCGGTCTCTATCGTGCATTCCATTATGGAGCACCACCGC
ATGGCGGTATGGCTGCGGGTGTTGATCGTATTATTATGCTTTTGCAAGGCGTCAAAAATTTACGTGAGGTTGCTTTATTT
CCTATGAATCAGCAAGCGCTTGATCTTTTGATGAGTGCTCCGTCTGACGTTTCTCCTGTGCAATTGCGTGATTTAGGGAT
ACGAATAGCTCCCGCTGCTAAGAATGATTAG

Upstream 100 bases:

>100_bases
TCTTGACTTTTTTTTTGAGTCTTTGTCCTCATATCTTGACAAATGGTTGTTAAAAAGCGTTTGTAGCGCATGTGAGTTAT
TATAATCAAAGGCAAAAATT

Downstream 100 bases:

>100_bases
TAAAGTTGCTTGTGTAATTATTATTGTCGTAAATATTTTTATTGTCTCCTGCATTTGGTAAAATTTAATAATTTAAAATT
ACTTTATTTTCAGTACGATA

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 596; Mature: 596

Protein sequence:

>596_residues
MHRYRSHHCAALRKCDVGIKARLSGWVHRVRDHGGILFVDLRDHFGITQIVADPASPAFKIIEKVRSEWVIRVDGEVRAR
SDEVINTSLPTGEIEIFAKEVEILSKSDELPLPVFGEPDYPEDIRLKYRFLDLRRETMHRNIMRRTEIIAAIRRSMQENG
FTEFTTPLLTASSPEGARDFLVPSRIHQGKFYALPQAPQQYKQLLMMSGFDRYFQIAPCFRDEDPRADRLPGEFYQLDVE
MSFVEQEDVLVTMEPIMRSLFEEFADGKPVTQSFPRLSYEEAMRKYGSDKPDLRNPIIMEDVSQHFYDSGFKVFAQILAN
DENARVWAIPAKTGGSRAFCDRMNGWAQSEGQPGLGYIFWREEQGKFEGAGPIAKNIGEQRTEALRMQLGLENGDACFFV
AGDPKKFLPFAGAARTRVGEELDLVDRDCFSLAWIVDFPFFEWNEEEKKLDFAHNPFSMPQGGKDALECQDPLTLKAFQY
DLVCNGYEIASGGIRNHSPEMMLKVFNLAGLSKEVVEDRFGGLYRAFHYGAPPHGGMAAGVDRIIMLLQGVKNLREVALF
PMNQQALDLLMSAPSDVSPVQLRDLGIRIAPAAKND

Sequences:

>Translated_596_residues
MHRYRSHHCAALRKCDVGIKARLSGWVHRVRDHGGILFVDLRDHFGITQIVADPASPAFKIIEKVRSEWVIRVDGEVRAR
SDEVINTSLPTGEIEIFAKEVEILSKSDELPLPVFGEPDYPEDIRLKYRFLDLRRETMHRNIMRRTEIIAAIRRSMQENG
FTEFTTPLLTASSPEGARDFLVPSRIHQGKFYALPQAPQQYKQLLMMSGFDRYFQIAPCFRDEDPRADRLPGEFYQLDVE
MSFVEQEDVLVTMEPIMRSLFEEFADGKPVTQSFPRLSYEEAMRKYGSDKPDLRNPIIMEDVSQHFYDSGFKVFAQILAN
DENARVWAIPAKTGGSRAFCDRMNGWAQSEGQPGLGYIFWREEQGKFEGAGPIAKNIGEQRTEALRMQLGLENGDACFFV
AGDPKKFLPFAGAARTRVGEELDLVDRDCFSLAWIVDFPFFEWNEEEKKLDFAHNPFSMPQGGKDALECQDPLTLKAFQY
DLVCNGYEIASGGIRNHSPEMMLKVFNLAGLSKEVVEDRFGGLYRAFHYGAPPHGGMAAGVDRIIMLLQGVKNLREVALF
PMNQQALDLLMSAPSDVSPVQLRDLGIRIAPAAKND
>Mature_596_residues
MHRYRSHHCAALRKCDVGIKARLSGWVHRVRDHGGILFVDLRDHFGITQIVADPASPAFKIIEKVRSEWVIRVDGEVRAR
SDEVINTSLPTGEIEIFAKEVEILSKSDELPLPVFGEPDYPEDIRLKYRFLDLRRETMHRNIMRRTEIIAAIRRSMQENG
FTEFTTPLLTASSPEGARDFLVPSRIHQGKFYALPQAPQQYKQLLMMSGFDRYFQIAPCFRDEDPRADRLPGEFYQLDVE
MSFVEQEDVLVTMEPIMRSLFEEFADGKPVTQSFPRLSYEEAMRKYGSDKPDLRNPIIMEDVSQHFYDSGFKVFAQILAN
DENARVWAIPAKTGGSRAFCDRMNGWAQSEGQPGLGYIFWREEQGKFEGAGPIAKNIGEQRTEALRMQLGLENGDACFFV
AGDPKKFLPFAGAARTRVGEELDLVDRDCFSLAWIVDFPFFEWNEEEKKLDFAHNPFSMPQGGKDALECQDPLTLKAFQY
DLVCNGYEIASGGIRNHSPEMMLKVFNLAGLSKEVVEDRFGGLYRAFHYGAPPHGGMAAGVDRIIMLLQGVKNLREVALF
PMNQQALDLLMSAPSDVSPVQLRDLGIRIAPAAKND

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI40789249, Length=600, Percent_Identity=35.6666666666667, Blast_Score=330, Evalue=2e-90,
Organism=Homo sapiens, GI45439306, Length=348, Percent_Identity=27.5862068965517, Blast_Score=96, Evalue=9e-20,
Organism=Homo sapiens, GI226491502, Length=253, Percent_Identity=23.3201581027668, Blast_Score=80, Evalue=7e-15,
Organism=Homo sapiens, GI5031815, Length=262, Percent_Identity=27.0992366412214, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI194272210, Length=262, Percent_Identity=27.0992366412214, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1788173, Length=597, Percent_Identity=42.5460636515913, Blast_Score=460, Evalue=1e-131,
Organism=Escherichia coli, GI1789256, Length=295, Percent_Identity=28.8135593220339, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1790571, Length=293, Percent_Identity=27.6450511945392, Blast_Score=83, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI32566633, Length=606, Percent_Identity=33.8283828382838, Blast_Score=289, Evalue=4e-78,
Organism=Caenorhabditis elegans, GI17551876, Length=264, Percent_Identity=25.3787878787879, Blast_Score=79, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6325153, Length=327, Percent_Identity=33.9449541284404, Blast_Score=162, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6323011, Length=261, Percent_Identity=30.6513409961686, Blast_Score=78, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24584738, Length=597, Percent_Identity=33.8358458961474, Blast_Score=291, Evalue=1e-78,
Organism=Drosophila melanogaster, GI17136276, Length=262, Percent_Identity=27.0992366412214, Blast_Score=80, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24640851, Length=251, Percent_Identity=24.7011952191235, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24640849, Length=251, Percent_Identity=24.7011952191235, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 67627; Mature: 67627

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRYRSHHCAALRKCDVGIKARLSGWVHRVRDHGGILFVDLRDHFGITQIVADPASPAFK
CCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHCCCCCHHHH
IIEKVRSEWVIRVDGEVRARSDEVINTSLPTGEIEIFAKEVEILSKSDELPLPVFGEPDY
HHHHHHHCEEEEECCCEECCCCCEEECCCCCCCEEEEHHHHHHHCCCCCCCCCCCCCCCC
PEDIRLKYRFLDLRRETMHRNIMRRTEIIAAIRRSMQENGFTEFTTPLLTASSPEGARDF
CHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCEECCCCCCCHHC
LVPSRIHQGKFYALPQAPQQYKQLLMMSGFDRYFQIAPCFRDEDPRADRLPGEFYQLDVE
CCCCHHCCCCEEECCCCHHHHHHHHHHHCCCHHEEECCCCCCCCCCCCCCCCCEEEEEEE
MSFVEQEDVLVTMEPIMRSLFEEFADGKPVTQSFPRLSYEEAMRKYGSDKPDLRNPIIME
EEEECCCCEEEEHHHHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHCCCCCCCCCCHHHH
DVSQHFYDSGFKVFAQILANDENARVWAIPAKTGGSRAFCDRMNGWAQSEGQPGLGYIFW
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHCCCCCCCCCCCCEEEEE
REEQGKFEGAGPIAKNIGEQRTEALRMQLGLENGDACFFVAGDPKKFLPFAGAARTRVGE
ECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHCCCCCCHHHHCCC
ELDLVDRDCFSLAWIVDFPFFEWNEEEKKLDFAHNPFSMPQGGKDALECQDPLTLKAFQY
HHHHHHHHHHHEEEEEECCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEE
DLVCNGYEIASGGIRNHSPEMMLKVFNLAGLSKEVVEDRFGGLYRAFHYGAPPHGGMAAG
EEEECCEEECCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHCCCCCCCCHHHH
VDRIIMLLQGVKNLREVALFPMNQQALDLLMSAPSDVSPVQLRDLGIRIAPAAKND
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCEEEEECCEEEECCCCCC
>Mature Secondary Structure
MHRYRSHHCAALRKCDVGIKARLSGWVHRVRDHGGILFVDLRDHFGITQIVADPASPAFK
CCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHCCCCCHHHH
IIEKVRSEWVIRVDGEVRARSDEVINTSLPTGEIEIFAKEVEILSKSDELPLPVFGEPDY
HHHHHHHCEEEEECCCEECCCCCEEECCCCCCCEEEEHHHHHHHCCCCCCCCCCCCCCCC
PEDIRLKYRFLDLRRETMHRNIMRRTEIIAAIRRSMQENGFTEFTTPLLTASSPEGARDF
CHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCEECCCCCCCHHC
LVPSRIHQGKFYALPQAPQQYKQLLMMSGFDRYFQIAPCFRDEDPRADRLPGEFYQLDVE
CCCCHHCCCCEEECCCCHHHHHHHHHHHCCCHHEEECCCCCCCCCCCCCCCCCEEEEEEE
MSFVEQEDVLVTMEPIMRSLFEEFADGKPVTQSFPRLSYEEAMRKYGSDKPDLRNPIIME
EEEECCCCEEEEHHHHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHCCCCCCCCCCHHHH
DVSQHFYDSGFKVFAQILANDENARVWAIPAKTGGSRAFCDRMNGWAQSEGQPGLGYIFW
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHCCCCCCCCCCCCEEEEE
REEQGKFEGAGPIAKNIGEQRTEALRMQLGLENGDACFFVAGDPKKFLPFAGAARTRVGE
ECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHCCCCCCHHHHCCC
ELDLVDRDCFSLAWIVDFPFFEWNEEEKKLDFAHNPFSMPQGGKDALECQDPLTLKAFQY
HHHHHHHHHHHEEEEEECCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEE
DLVCNGYEIASGGIRNHSPEMMLKVFNLAGLSKEVVEDRFGGLYRAFHYGAPPHGGMAAG
EEEECCEEECCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHCCCCCCCCHHHH
VDRIIMLLQGVKNLREVALFPMNQQALDLLMSAPSDVSPVQLRDLGIRIAPAAKND
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCEEEEECCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA