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The map label for this gene is nuoM [H]

Identifier: 49475646

GI number: 49475646

Start: 998001

End: 999470

Strand: Reverse

Name: nuoM [H]

Synonym: BH08830

Alternate gene names: 49475646

Gene position: 999470-998001 (Counterclockwise)

Preceding gene: 49475647

Following gene: 49475645

Centisome position: 51.76

GC content: 36.73

Gene sequence:

>1470_bases
ATGACTGATTGGCCTATTCTTTCTACGGTTACATTTTTGCCGCTTGTTGGTGTAATTTTGATTCTCTTTATCAAAGATGA
TAGCGAGGCAGCACGATATAATATACGCAATGTAGCGTTTTTTACGACTGTGTTTGTTTTTATTATTTCCTTAATTATTT
GGGCTGGTTTTGATTATACAAATGCTCATTTTCAAATGGTTGAGAAATTCAATTGGTTAGGAGGGGGCATTAGCTATCAT
ATGGGGGTTGATGGTATTTCCATTCTTTTTGTTGTTCTTTCAGCTTTTCTTTTGCCTTTCTGTATTTTAGCGAGCTGGGA
AAATATTAAAGATAGATTAAAAGCTTATATGATTGCTTTTCTTCTTCTTGAAGTTGCAATTATTGGCGTTTTTTGTGCTC
TTGATGCAATATTGTTTTATGTTTTTTTTGAAGGTAGCCTTATTCCGATGTTTATTATTATAGGCGTTTGGGGTGGAGCA
CGTCGTGTTTATGCGAGTATGAAGTTTTTTCTTTATACTTTACTTGGGTCAGTGCTTATGCTGGTTGCCATTATGGTTAT
GTATTGGGAAACAGGTACTCTCGATATACCAACTTTACTCAATTATCAGTTTCCAGCGCATATGCAAATATGGTTATGGC
TTGCATTTTTTGCTTCTTTTGCCGTTAAAATGCCGATGTGGCCAGTTCATACATGGCTCCCTGATGCTCACGTGGAAGCA
CCAACAGCTGGTTCCGTTATTTTAGCCGGTGTTTTACTCAAATTAGGCGGGTATGGTTTCCTTCGTTTTTCTTTACCAAT
GTTTCCTCTTGCTTCAGCTGATTTTGCGCCTTTGGTTTTTACTTTATCGGTTAGCGCTATCATTTATACATCGTTGGTTG
CGCTTGTTCAAAATGACATAAAAAAACTTATTGCTTATTCCTCAATAGCGCATATGGGGTATGTGACGATGGGCATTTTT
GCCGCCAATGAACAGGCTATCCAAGGTGCTATTTATCAAATGCTATCGCATGGAATTGTTTCAGCAGCTTTATTTCTTTG
TGTTGGAGTTATCTACGATCGCTTGCATACACGTGAAATTTCAGCTTTTGGCGGTTTAGTGAATAACATGCCTAAATATG
CCGTTATTTTCTTGATTTTCACTATGGCAAATGTCGGTTTGCCGGGAAGTTCAGGATTTTTAGGTGAGTTTTTAACCTTA
ATAGGCGTTTTTCAAGTGAATAAATTGGTCGTTATTTTTGCTACAACTGGCGTTATTTTATCAGCTGCTTATGCACTTTA
TCTTTATCGGCGTGTGGTTTTTGGTTCTTTGGATAAAGAGAATTTAAAAGTCCTTATTGATCTCTCTTCAAGAGAAAAAT
TCATTCTTTATCCGATGGTTATTCTTACAATATTTTTTGGTATTTATCCAACGCCTATTCTTAAAGCAACGGCGTTTTCC
GTGAAAGCTCTCATCAATAAACTACACTAG

Upstream 100 bases:

>100_bases
TTATTCGGTTGCAGACAGGCTATCTTTATCATTATGCATTTGTAATGCTTATTGGCATTGCATCGCTGATCACATGGATG
ATGATCGGGGGCGTAAACTG

Downstream 100 bases:

>100_bases
ATAAGAGAAGAATGCTCATGCAAACTGAAATAATCACTCAATTAGTGTTAATTTTTCCAGAGATTTTAATAGCTTTGGGG
GGAATCACGTTACTTTTGTT

Product: NADH dehydrogenase subunit M

Products: NA

Alternate protein names: NADH dehydrogenase I, chain 13; NDH-1, chain 13 [H]

Number of amino acids: Translated: 489; Mature: 488

Protein sequence:

>489_residues
MTDWPILSTVTFLPLVGVILILFIKDDSEAARYNIRNVAFFTTVFVFIISLIIWAGFDYTNAHFQMVEKFNWLGGGISYH
MGVDGISILFVVLSAFLLPFCILASWENIKDRLKAYMIAFLLLEVAIIGVFCALDAILFYVFFEGSLIPMFIIIGVWGGA
RRVYASMKFFLYTLLGSVLMLVAIMVMYWETGTLDIPTLLNYQFPAHMQIWLWLAFFASFAVKMPMWPVHTWLPDAHVEA
PTAGSVILAGVLLKLGGYGFLRFSLPMFPLASADFAPLVFTLSVSAIIYTSLVALVQNDIKKLIAYSSIAHMGYVTMGIF
AANEQAIQGAIYQMLSHGIVSAALFLCVGVIYDRLHTREISAFGGLVNNMPKYAVIFLIFTMANVGLPGSSGFLGEFLTL
IGVFQVNKLVVIFATTGVILSAAYALYLYRRVVFGSLDKENLKVLIDLSSREKFILYPMVILTIFFGIYPTPILKATAFS
VKALINKLH

Sequences:

>Translated_489_residues
MTDWPILSTVTFLPLVGVILILFIKDDSEAARYNIRNVAFFTTVFVFIISLIIWAGFDYTNAHFQMVEKFNWLGGGISYH
MGVDGISILFVVLSAFLLPFCILASWENIKDRLKAYMIAFLLLEVAIIGVFCALDAILFYVFFEGSLIPMFIIIGVWGGA
RRVYASMKFFLYTLLGSVLMLVAIMVMYWETGTLDIPTLLNYQFPAHMQIWLWLAFFASFAVKMPMWPVHTWLPDAHVEA
PTAGSVILAGVLLKLGGYGFLRFSLPMFPLASADFAPLVFTLSVSAIIYTSLVALVQNDIKKLIAYSSIAHMGYVTMGIF
AANEQAIQGAIYQMLSHGIVSAALFLCVGVIYDRLHTREISAFGGLVNNMPKYAVIFLIFTMANVGLPGSSGFLGEFLTL
IGVFQVNKLVVIFATTGVILSAAYALYLYRRVVFGSLDKENLKVLIDLSSREKFILYPMVILTIFFGIYPTPILKATAFS
VKALINKLH
>Mature_488_residues
TDWPILSTVTFLPLVGVILILFIKDDSEAARYNIRNVAFFTTVFVFIISLIIWAGFDYTNAHFQMVEKFNWLGGGISYHM
GVDGISILFVVLSAFLLPFCILASWENIKDRLKAYMIAFLLLEVAIIGVFCALDAILFYVFFEGSLIPMFIIIGVWGGAR
RVYASMKFFLYTLLGSVLMLVAIMVMYWETGTLDIPTLLNYQFPAHMQIWLWLAFFASFAVKMPMWPVHTWLPDAHVEAP
TAGSVILAGVLLKLGGYGFLRFSLPMFPLASADFAPLVFTLSVSAIIYTSLVALVQNDIKKLIAYSSIAHMGYVTMGIFA
ANEQAIQGAIYQMLSHGIVSAALFLCVGVIYDRLHTREISAFGGLVNNMPKYAVIFLIFTMANVGLPGSSGFLGEFLTLI
GVFQVNKLVVIFATTGVILSAAYALYLYRRVVFGSLDKENLKVLIDLSSREKFILYPMVILTIFFGIYPTPILKATAFSV
KALINKLH

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=340, Percent_Identity=37.6470588235294, Blast_Score=206, Evalue=3e-53,
Organism=Homo sapiens, GI251831117, Length=319, Percent_Identity=27.5862068965517, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1788613, Length=471, Percent_Identity=39.2781316348195, Blast_Score=323, Evalue=2e-89,
Organism=Escherichia coli, GI1788827, Length=316, Percent_Identity=28.1645569620253, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI1788614, Length=493, Percent_Identity=25.7606490872211, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1788831, Length=295, Percent_Identity=27.1186440677966, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI1788829, Length=407, Percent_Identity=24.0786240786241, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI145693160, Length=424, Percent_Identity=24.2924528301887, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI2367154, Length=259, Percent_Identity=26.6409266409266, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918
- InterPro:   IPR000260 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01059 Oxidored_q5_N [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 54423; Mature: 54292

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHCC
>Mature Secondary Structure 
TDWPILSTVTFLPLVGVILILFIKDDSEAARYNIRNVAFFTTVFVFIISLIIWAGFDYT
CCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
NAHFQMVEKFNWLGGGISYHMGVDGISILFVVLSAFLLPFCILASWENIKDRLKAYMIAF
HHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLLEVAIIGVFCALDAILFYVFFEGSLIPMFIIIGVWGGARRVYASMKFFLYTLLGSVLM
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LVAIMVMYWETGTLDIPTLLNYQFPAHMQIWLWLAFFASFAVKMPMWPVHTWLPDAHVEA
HHHHHHHHHCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCC
PTAGSVILAGVLLKLGGYGFLRFSLPMFPLASADFAPLVFTLSVSAIIYTSLVALVQNDI
CCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KKLIAYSSIAHMGYVTMGIFAANEQAIQGAIYQMLSHGIVSAALFLCVGVIYDRLHTREI
HHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAFGGLVNNMPKYAVIFLIFTMANVGLPGSSGFLGEFLTLIGVFQVNKLVVIFATTGVIL
HHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCEEEEEEHHHHHH
SAAYALYLYRRVVFGSLDKENLKVLIDLSSREKFILYPMVILTIFFGIYPTPILKATAFS
HHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCEEHHHHHHHHHHHHHCCCHHHHHHHHH
VKALINKLH
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8422400 [H]