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The map label for this gene is proS

Identifier: 49475642

GI number: 49475642

Start: 992529

End: 993854

Strand: Reverse

Name: proS

Synonym: BH08790

Alternate gene names: 49475642

Gene position: 993854-992529 (Counterclockwise)

Preceding gene: 49475643

Following gene: 49475641

Centisome position: 51.47

GC content: 36.65

Gene sequence:

>1326_bases
ATGCGTCTTTCTCAATATTTCCTTCCACTTTTAAAAGAGAATCCTAAGGAAGCAGAAATTATTTCTCATCGACTCATGTT
GCGTGCAGGTATGATTCGTCAGCAAACATCAGGGATTTATTCTTGGCTTCCTTTGGGTAAAAAAGTGCTTGATAAAATTT
GTAAAATTATTCGTGAGGAGCAAGAACGAGCTGGTGCTATAGAAATATTAATGCCTACAATTCAATCTGCGGATCTTTGG
CGTGAAAGTGATCGTTATGATGATTATGGTTTAGAAATGCTGCGTATTAAAGATCGTCAAAAACGCGATTTACTTTATGG
TCCGACAAACGAAGAGATGGTAACAGATATATTCCGTTCCTATATTCGTTCTTATAAAGATCTTCCGCTTAATTTGTATC
ATATTCAATGGAAGTTTCGTGATGAAATCCGTCCACGTTTTGGTGTAATGCGCTCACGGGAATTTTTAATGAAAGATGCT
TATTCTTTTGATCTTGATTATGAGGGTTCTAAAATATCTTATAATCGTATGTTTGTTGCTTATTTACGCACTTTTTCTTG
TCTTGGCTTAAAAGCAATTCCTATGCGTGCTGATACAGGTCCAATAGGTGGTAAACTCAGTCATGAGTTTATTATTTTGG
CTGAAACGGGAGAGAGCGCTATCTTCTGTGATAAACACTTTCTTGAACTTACTGTTCCAGACAGTTCGATAGATTTTAGT
GATAAAGCTATTTTAGCCAATATTGTTAAACAGTGGACCTCTTTCTATGCAGCAACAGAGGAAATGCACGATGAAGAAGA
GTGGGCTAAGATTTCTGATAATAATCGCCTCTCAGCCCGTGGTATTGAAGTAGGACATATTTTCCATTTTGGTACAAAAT
ATTCTGCTCCAATGGGAGCAAAAGTTATGGGACAAGATGGAAAAGAGCATTTGGTTTCTATGGGATCCTATGGTATTGGG
CCTTCACGTCTTGTTGCCGCAGTTATTGAAGCTTCTCATGATGAGAATGGTATTATTTGGCCAAAGTCGATAGCTCCGTT
TGATTTTGGTATCATCAATATGAAACCAGATGATGAAAAATGTACACATGCGTGTGAAACTCTCTATAAAGGCTTAAGGT
ATGCTGGTTTTGATCCATTATTAGATGATAGAAATGAACGTCCTGGATCAAAATTTGCAACAATGGATTTGATTGGTCTG
CCAACACAAATAATTGTTGGGCCTAAAAGTGTTGCACAAAATGAAGTTGAGATTAAAGATAGAAAAACGGGTATAAAAAA
ATCTCTAACGGTTGAAAATGTACTCAACCAGTTTTCTGTAATTTGA

Upstream 100 bases:

>100_bases
AATTGTTATGTTTGCAAGTATTCAGAATGTAGCTATAGGCGAGTGTAGAGTATATTTAAATTATTCCGATATGATAGGCT
TAATGAGGAGTTAATTATTA

Downstream 100 bases:

>100_bases
TAGGCAGTGTTATGAAGTGGTTGAGGAGTAAATGGTTTTCATCTTATGAATGGATGATCGCTTTTCGTTATATGATTCCT
AATAAAAAACACGTAGTTGC

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MRLSQYFLPLLKENPKEAEIISHRLMLRAGMIRQQTSGIYSWLPLGKKVLDKICKIIREEQERAGAIEILMPTIQSADLW
RESDRYDDYGLEMLRIKDRQKRDLLYGPTNEEMVTDIFRSYIRSYKDLPLNLYHIQWKFRDEIRPRFGVMRSREFLMKDA
YSFDLDYEGSKISYNRMFVAYLRTFSCLGLKAIPMRADTGPIGGKLSHEFIILAETGESAIFCDKHFLELTVPDSSIDFS
DKAILANIVKQWTSFYAATEEMHDEEEWAKISDNNRLSARGIEVGHIFHFGTKYSAPMGAKVMGQDGKEHLVSMGSYGIG
PSRLVAAVIEASHDENGIIWPKSIAPFDFGIINMKPDDEKCTHACETLYKGLRYAGFDPLLDDRNERPGSKFATMDLIGL
PTQIIVGPKSVAQNEVEIKDRKTGIKKSLTVENVLNQFSVI

Sequences:

>Translated_441_residues
MRLSQYFLPLLKENPKEAEIISHRLMLRAGMIRQQTSGIYSWLPLGKKVLDKICKIIREEQERAGAIEILMPTIQSADLW
RESDRYDDYGLEMLRIKDRQKRDLLYGPTNEEMVTDIFRSYIRSYKDLPLNLYHIQWKFRDEIRPRFGVMRSREFLMKDA
YSFDLDYEGSKISYNRMFVAYLRTFSCLGLKAIPMRADTGPIGGKLSHEFIILAETGESAIFCDKHFLELTVPDSSIDFS
DKAILANIVKQWTSFYAATEEMHDEEEWAKISDNNRLSARGIEVGHIFHFGTKYSAPMGAKVMGQDGKEHLVSMGSYGIG
PSRLVAAVIEASHDENGIIWPKSIAPFDFGIINMKPDDEKCTHACETLYKGLRYAGFDPLLDDRNERPGSKFATMDLIGL
PTQIIVGPKSVAQNEVEIKDRKTGIKKSLTVENVLNQFSVI
>Mature_441_residues
MRLSQYFLPLLKENPKEAEIISHRLMLRAGMIRQQTSGIYSWLPLGKKVLDKICKIIREEQERAGAIEILMPTIQSADLW
RESDRYDDYGLEMLRIKDRQKRDLLYGPTNEEMVTDIFRSYIRSYKDLPLNLYHIQWKFRDEIRPRFGVMRSREFLMKDA
YSFDLDYEGSKISYNRMFVAYLRTFSCLGLKAIPMRADTGPIGGKLSHEFIILAETGESAIFCDKHFLELTVPDSSIDFS
DKAILANIVKQWTSFYAATEEMHDEEEWAKISDNNRLSARGIEVGHIFHFGTKYSAPMGAKVMGQDGKEHLVSMGSYGIG
PSRLVAAVIEASHDENGIIWPKSIAPFDFGIINMKPDDEKCTHACETLYKGLRYAGFDPLLDDRNERPGSKFATMDLIGL
PTQIIVGPKSVAQNEVEIKDRKTGIKKSLTVENVLNQFSVI

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro)

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 2 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=441, Percent_Identity=31.9727891156463, Blast_Score=231, Evalue=1e-60,
Organism=Escherichia coli, GI1786392, Length=263, Percent_Identity=52.4714828897338, Blast_Score=278, Evalue=5e-76,
Organism=Caenorhabditis elegans, GI115532348, Length=401, Percent_Identity=32.1695760598504, Blast_Score=195, Evalue=3e-50,
Organism=Caenorhabditis elegans, GI193203271, Length=97, Percent_Identity=32.9896907216495, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320931, Length=242, Percent_Identity=35.5371900826446, Blast_Score=166, Evalue=6e-42,
Organism=Drosophila melanogaster, GI24656200, Length=425, Percent_Identity=31.7647058823529, Blast_Score=211, Evalue=8e-55,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_BARHE (Q6G3A4)

Other databases:

- EMBL:   BX897699
- RefSeq:   YP_033683.1
- HSSP:   O26708
- ProteinModelPortal:   Q6G3A4
- SMR:   Q6G3A4
- GeneID:   2865533
- GenomeReviews:   BX897699_GR
- KEGG:   bhe:BH08790
- NMPDR:   fig|283166.1.peg.811
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- PhylomeDB:   Q6G3A4
- ProtClustDB:   PRK12325
- BioCyc:   BHEN283166:BH08790-MONOMER
- BRENDA:   6.1.1.15
- GO:   GO:0005737
- HAMAP:   MF_01570
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- Gene3D:   G3DSA:3.40.50.800
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.15

Molecular weight: Translated: 50440; Mature: 50440

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLSQYFLPLLKENPKEAEIISHRLMLRAGMIRQQTSGIYSWLPLGKKVLDKICKIIREE
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHH
QERAGAIEILMPTIQSADLWRESDRYDDYGLEMLRIKDRQKRDLLYGPTNEEMVTDIFRS
HHHCCEEEEEEHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCEECCCCHHHHHHHHHH
YIRSYKDLPLNLYHIQWKFRDEIRPRFGVMRSREFLMKDAYSFDLDYEGSKISYNRMFVA
HHHHHCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHH
YLRTFSCLGLKAIPMRADTGPIGGKLSHEFIILAETGESAIFCDKHFLELTVPDSSIDFS
HHHHHHHCCCEEECCCCCCCCCCCCCCCCEEEEEECCCCEEEECCEEEEEEECCCCCCCC
DKAILANIVKQWTSFYAATEEMHDEEEWAKISDNNRLSARGIEVGHIFHFGTKYSAPMGA
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCEEECCCEEEEEEEECCCCCCCCCC
KVMGQDGKEHLVSMGSYGIGPSRLVAAVIEASHDENGIIWPKSIAPFDFGIINMKPDDEK
EEECCCCHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCEEECCCCCCCCCEEEECCCCHHH
CTHACETLYKGLRYAGFDPLLDDRNERPGSKFATMDLIGLPTQIIVGPKSVAQNEVEIKD
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEECCHHHCCCCCEEHH
RKTGIKKSLTVENVLNQFSVI
HHCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRLSQYFLPLLKENPKEAEIISHRLMLRAGMIRQQTSGIYSWLPLGKKVLDKICKIIREE
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHH
QERAGAIEILMPTIQSADLWRESDRYDDYGLEMLRIKDRQKRDLLYGPTNEEMVTDIFRS
HHHCCEEEEEEHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCEECCCCHHHHHHHHHH
YIRSYKDLPLNLYHIQWKFRDEIRPRFGVMRSREFLMKDAYSFDLDYEGSKISYNRMFVA
HHHHHCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHH
YLRTFSCLGLKAIPMRADTGPIGGKLSHEFIILAETGESAIFCDKHFLELTVPDSSIDFS
HHHHHHHCCCEEECCCCCCCCCCCCCCCCEEEEEECCCCEEEECCEEEEEEECCCCCCCC
DKAILANIVKQWTSFYAATEEMHDEEEWAKISDNNRLSARGIEVGHIFHFGTKYSAPMGA
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCEEECCCEEEEEEEECCCCCCCCCC
KVMGQDGKEHLVSMGSYGIGPSRLVAAVIEASHDENGIIWPKSIAPFDFGIINMKPDDEK
EEECCCCHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCEEECCCCCCCCCEEEECCCCHHH
CTHACETLYKGLRYAGFDPLLDDRNERPGSKFATMDLIGLPTQIIVGPKSVAQNEVEIKD
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEECCHHHCCCCCEEHH
RKTGIKKSLTVENVLNQFSVI
HHCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA