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The map label for this gene is vacB [H]

Identifier: 49475580

GI number: 49475580

Start: 917559

End: 919832

Strand: Reverse

Name: vacB [H]

Synonym: BH08150

Alternate gene names: 49475580

Gene position: 919832-917559 (Counterclockwise)

Preceding gene: 49475581

Following gene: 49475579

Centisome position: 47.63

GC content: 35.58

Gene sequence:

>2274_bases
TTGGCTAAAGGTGAAAAAAAAACACAATATTTTCAACCGCTGAACTTGGGACGGCTGCCTAATAAAGCAGAAATTCTCTC
CTTTATTAATGAGAATCCTGGTCGATCAAGTAAACGTGAAATAGCAAAAGCTTTTAATTTAAAAAATGATTCTCGTATTT
GGTTACGAGATATATTGCGTGATCTGAAAAATCAGAGCTTAGGAGCAAAGGAGCGTAAAAAGGCAAGAAATAAAGGACAA
TTACCAGCGGTTGCTATTTTAAAAATTACTGCACGTGATGAAGACGGTAGTTTTATTGCACAACCTCTTGAGTGGGAAGA
TGGTCCAAAACCAAAGATTGAAATACTCCCTTTTCATCGTACAAAAGGAATAAATGTTGGTGTTGGAGACCATATTCTTG
TAAAAATTTTTCGAAATAAAGTGCCTCAAAGCCTCCCTTATACAGGGAAAATTATTCGTAAAATTGATGTATCACCAAAG
AGCACATTTGGCATTGTACGAAAATTAGAAAATGGTCAATGGCGTCTTGATTCTGTTGAGCGCAAAACAAGCGAATTGAT
TATTGATACATCTTCAGAAATTCATGTACAATCCGGCGATTTTGTTGAAGTCGAAATTAAAAAAAATACTGGTTATGGGC
TAAAGAGTGCACAAATAAAAAATGTTTTAGGACATATTGAGAGTGAAAAAACACTTTCAATGATTGCTCTTCTGTCAAAA
GGAATTCCTTATATTTTTCCTGAAGATGTTCTTAAACAAGTCAAAAATGTAAAACCTGCCAGCATAGAAAATCGTGAAGA
TTGGCGGCAATTGCCACTCATTACGATTGATCCACCAGATGCAAAAGATCGTGATGATGCAGTTTATGCAACAAAAGACA
AAGATCCTACAAATAGTGGCGGTTGGATTATTGTTGTCGCAATCGCAGATGTTTCTTACTATGTCAAAACCGGAAGTGCT
ATAGATAAAGAAGCATTGAAGCGAGGAAATTCTGTTTATTTTCCTGATACCGTTGTGCCTATGCTTCCAGAACGTATTTC
TAATAATTTATGTTCTCTGCATGAAGGAAAAGAGAAACCCGCTTTAGCAGTTCGTATGATTTTTGATGCAAACGGTAATA
AACGAAAACATAGTTTTCATCGTGTTATGGTACGTATAAGAGCTAACCTCTCTTATCAAGAAGTTCAGTCAGCAATTGAG
GGAAATATAAATGAAAAAATAGCTCCTCTTTTCGAAAATGTCTTACAACCATTGTGGAAAGCTTATGAATGCCTTAAAAT
TACACGGGATCGTCGACAACCACTAGAATTAGAAATAGCAGAAAAAAAAATTATTCTTGATCAAAATGGATGCATTAAAG
ATGTTGAAATACAAACATACCTAGAAGCACATCGTTTGATTGAAGAACTCATGATACAAGCTAATATTGCAGCATCTGAA
ACATTAAAAGAACATCACCAGCCCCTCATCTATCGAATTCATGATAAACCCTCTCTTGCAAAACAAGAAATACTGCGGAG
TTTTCTTCAAAGTTTGGGTTTATCACTCTCTCGAGGCGCAGAACTGACATCAGCCCGTCTTAATAGAATTTTAGAAAAGG
TTATGAATACCCAGCAACAAGAATTAGTGAATCAGGTTATTTTGCGCACACAATCTCAAGCAGAATATAACCCCCAAAAT
ATCGGCCATTTTGGTTTAAACTTACATAACTATACACATTTTACATCACCAATTCGTCGTTATGCTGATCTGGTTATTCA
TCGAGCACTCATTAAAGCTCTAAAGTTAGGGAATGACCAATTAACAGAGACACAAGAAAAAAATCTTGCAGAAATCGCCA
CACAAATTTCTCTCTATGAACGCCGAGCAATGATGGCTGAAAGAGAAACGATTGATAGGCTTGTCGCACATTATTTAACC
AATAAAATCGGCTGCACTTTTACAGGTCGTGTTTCTGGAGTTACAAAAGCAGGTCTTTTTATTTCACTTGACACACTCAA
TATAGATGGTTTTGTGCATATTTCTACACTTAAAAATGATTATTATTATTTCGATGAAGTGCAACATACCCTTGTAGGAA
AGCGTAGTCATAAAGGCTATCAACTCACTGATATAGTAAAAGTGAAGCTTATAAAAGTACAGCCCTTTGCTGGTGCTTTA
TGTTTTGAACTTTTAACAGATCCTCGCCCAATAGCTTTTTCATCTGCGTCCTACCATAAAAACAAATTTATAGCGAAAAA
ACAAAAGCGATATGCAAGAAACAATATCAGATAA

Upstream 100 bases:

>100_bases
TGTGACACTTTCAGAAGCATTAGAACTTATATCGGTTAAAGCATCCGGTAAAAAAACAACCTTAAAAAGAGCTTCTACTA
AAACAAAAGAGACATAAATT

Downstream 100 bases:

>100_bases
AAACCAAGAAAATAGGTAAAAACGAAAATCATCACGATAATTTTATTATACTCTCAAAAGATAAAAAATCTTATGAGAGA
TAATTAGAGTGTTATTAATT

Product: VacB and RNase II family exoribonuclease

Products: NA

Alternate protein names: RNase R; VacB protein homolog [H]

Number of amino acids: Translated: 757; Mature: 756

Protein sequence:

>757_residues
MAKGEKKTQYFQPLNLGRLPNKAEILSFINENPGRSSKREIAKAFNLKNDSRIWLRDILRDLKNQSLGAKERKKARNKGQ
LPAVAILKITARDEDGSFIAQPLEWEDGPKPKIEILPFHRTKGINVGVGDHILVKIFRNKVPQSLPYTGKIIRKIDVSPK
STFGIVRKLENGQWRLDSVERKTSELIIDTSSEIHVQSGDFVEVEIKKNTGYGLKSAQIKNVLGHIESEKTLSMIALLSK
GIPYIFPEDVLKQVKNVKPASIENREDWRQLPLITIDPPDAKDRDDAVYATKDKDPTNSGGWIIVVAIADVSYYVKTGSA
IDKEALKRGNSVYFPDTVVPMLPERISNNLCSLHEGKEKPALAVRMIFDANGNKRKHSFHRVMVRIRANLSYQEVQSAIE
GNINEKIAPLFENVLQPLWKAYECLKITRDRRQPLELEIAEKKIILDQNGCIKDVEIQTYLEAHRLIEELMIQANIAASE
TLKEHHQPLIYRIHDKPSLAKQEILRSFLQSLGLSLSRGAELTSARLNRILEKVMNTQQQELVNQVILRTQSQAEYNPQN
IGHFGLNLHNYTHFTSPIRRYADLVIHRALIKALKLGNDQLTETQEKNLAEIATQISLYERRAMMAERETIDRLVAHYLT
NKIGCTFTGRVSGVTKAGLFISLDTLNIDGFVHISTLKNDYYYFDEVQHTLVGKRSHKGYQLTDIVKVKLIKVQPFAGAL
CFELLTDPRPIAFSSASYHKNKFIAKKQKRYARNNIR

Sequences:

>Translated_757_residues
MAKGEKKTQYFQPLNLGRLPNKAEILSFINENPGRSSKREIAKAFNLKNDSRIWLRDILRDLKNQSLGAKERKKARNKGQ
LPAVAILKITARDEDGSFIAQPLEWEDGPKPKIEILPFHRTKGINVGVGDHILVKIFRNKVPQSLPYTGKIIRKIDVSPK
STFGIVRKLENGQWRLDSVERKTSELIIDTSSEIHVQSGDFVEVEIKKNTGYGLKSAQIKNVLGHIESEKTLSMIALLSK
GIPYIFPEDVLKQVKNVKPASIENREDWRQLPLITIDPPDAKDRDDAVYATKDKDPTNSGGWIIVVAIADVSYYVKTGSA
IDKEALKRGNSVYFPDTVVPMLPERISNNLCSLHEGKEKPALAVRMIFDANGNKRKHSFHRVMVRIRANLSYQEVQSAIE
GNINEKIAPLFENVLQPLWKAYECLKITRDRRQPLELEIAEKKIILDQNGCIKDVEIQTYLEAHRLIEELMIQANIAASE
TLKEHHQPLIYRIHDKPSLAKQEILRSFLQSLGLSLSRGAELTSARLNRILEKVMNTQQQELVNQVILRTQSQAEYNPQN
IGHFGLNLHNYTHFTSPIRRYADLVIHRALIKALKLGNDQLTETQEKNLAEIATQISLYERRAMMAERETIDRLVAHYLT
NKIGCTFTGRVSGVTKAGLFISLDTLNIDGFVHISTLKNDYYYFDEVQHTLVGKRSHKGYQLTDIVKVKLIKVQPFAGAL
CFELLTDPRPIAFSSASYHKNKFIAKKQKRYARNNIR
>Mature_756_residues
AKGEKKTQYFQPLNLGRLPNKAEILSFINENPGRSSKREIAKAFNLKNDSRIWLRDILRDLKNQSLGAKERKKARNKGQL
PAVAILKITARDEDGSFIAQPLEWEDGPKPKIEILPFHRTKGINVGVGDHILVKIFRNKVPQSLPYTGKIIRKIDVSPKS
TFGIVRKLENGQWRLDSVERKTSELIIDTSSEIHVQSGDFVEVEIKKNTGYGLKSAQIKNVLGHIESEKTLSMIALLSKG
IPYIFPEDVLKQVKNVKPASIENREDWRQLPLITIDPPDAKDRDDAVYATKDKDPTNSGGWIIVVAIADVSYYVKTGSAI
DKEALKRGNSVYFPDTVVPMLPERISNNLCSLHEGKEKPALAVRMIFDANGNKRKHSFHRVMVRIRANLSYQEVQSAIEG
NINEKIAPLFENVLQPLWKAYECLKITRDRRQPLELEIAEKKIILDQNGCIKDVEIQTYLEAHRLIEELMIQANIAASET
LKEHHQPLIYRIHDKPSLAKQEILRSFLQSLGLSLSRGAELTSARLNRILEKVMNTQQQELVNQVILRTQSQAEYNPQNI
GHFGLNLHNYTHFTSPIRRYADLVIHRALIKALKLGNDQLTETQEKNLAEIATQISLYERRAMMAERETIDRLVAHYLTN
KIGCTFTGRVSGVTKAGLFISLDTLNIDGFVHISTLKNDYYYFDEVQHTLVGKRSHKGYQLTDIVKVKLIKVQPFAGALC
FELLTDPRPIAFSSASYHKNKFIAKKQKRYARNNIR

Specific function: 3'-5'exoribonuclease that participates in an essential cell function. Acts nonspecifically on poly(A), poly(U) and ribosomal RNAs [H]

COG id: COG0557

COG function: function code K; Exoribonuclease R

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI190014625, Length=426, Percent_Identity=28.8732394366197, Blast_Score=160, Evalue=4e-39,
Organism=Homo sapiens, GI190014623, Length=426, Percent_Identity=28.8732394366197, Blast_Score=160, Evalue=5e-39,
Organism=Homo sapiens, GI134288890, Length=393, Percent_Identity=30.0254452926209, Blast_Score=156, Evalue=6e-38,
Organism=Homo sapiens, GI219521928, Length=476, Percent_Identity=28.1512605042017, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI19115966, Length=476, Percent_Identity=28.1512605042017, Blast_Score=135, Evalue=1e-31,
Organism=Escherichia coli, GI87082383, Length=564, Percent_Identity=37.2340425531915, Blast_Score=352, Evalue=6e-98,
Organism=Escherichia coli, GI1787542, Length=467, Percent_Identity=29.5503211991435, Blast_Score=162, Evalue=8e-41,
Organism=Caenorhabditis elegans, GI212645896, Length=473, Percent_Identity=29.3868921775898, Blast_Score=169, Evalue=5e-42,
Organism=Caenorhabditis elegans, GI17553506, Length=456, Percent_Identity=28.5087719298246, Blast_Score=162, Evalue=9e-40,
Organism=Saccharomyces cerevisiae, GI6324552, Length=440, Percent_Identity=30.4545454545455, Blast_Score=171, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6320499, Length=291, Percent_Identity=24.7422680412371, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24649634, Length=425, Percent_Identity=27.5294117647059, Blast_Score=143, Evalue=4e-34,
Organism=Drosophila melanogaster, GI19922976, Length=360, Percent_Identity=29.4444444444444, Blast_Score=130, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24654597, Length=360, Percent_Identity=29.4444444444444, Blast_Score=130, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24654592, Length=360, Percent_Identity=29.4444444444444, Blast_Score=130, Evalue=4e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013223
- InterPro:   IPR001900
- InterPro:   IPR022966
- InterPro:   IPR004476
- InterPro:   IPR011805
- InterPro:   IPR013668 [H]

Pfam domain/function: PF08461 HTH_12; PF08206 OB_RNB; PF00773 RNB; PF00575 S1 [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 86181; Mature: 86049

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: PS50126 S1 ; PS01175 RIBONUCLEASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKGEKKTQYFQPLNLGRLPNKAEILSFINENPGRSSKREIAKAFNLKNDSRIWLRDILR
CCCCCCCHHHHCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHH
DLKNQSLGAKERKKARNKGQLPAVAILKITARDEDGSFIAQPLEWEDGPKPKIEILPFHR
HHHCCCCCCHHHHHHHCCCCCCEEEEEEEEECCCCCCEEECCCCCCCCCCCCEEEEEEEC
TKGINVGVGDHILVKIFRNKVPQSLPYTGKIIRKIDVSPKSTFGIVRKLENGQWRLDSVE
CCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCEEEHHHH
RKTSELIIDTSSEIHVQSGDFVEVEIKKNTGYGLKSAQIKNVLGHIESEKTLSMIALLSK
HHCHHEEEECCCEEEEECCCEEEEEEECCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHC
GIPYIFPEDVLKQVKNVKPASIENREDWRQLPLITIDPPDAKDRDDAVYATKDKDPTNSG
CCCCCCCHHHHHHHHCCCCCCCCCCHHHHHCCEEEECCCCCCCCCCCEEEECCCCCCCCC
GWIIVVAIADVSYYVKTGSAIDKEALKRGNSVYFPDTVVPMLPERISNNLCSLHEGKEKP
CEEEEEEEECCEEEEECCCCCCHHHHHCCCCEECCCCHHHHCHHHHCCCHHHHCCCCCCC
ALAVRMIFDANGNKRKHSFHRVMVRIRANLSYQEVQSAIEGNINEKIAPLFENVLQPLWK
CEEEEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
AYECLKITRDRRQPLELEIAEKKIILDQNGCIKDVEIQTYLEAHRLIEELMIQANIAASE
HHHHHHHHHHCCCCCEEEEECEEEEECCCCCEECCHHHHHHHHHHHHHHHHHHHCCHHHH
TLKEHHQPLIYRIHDKPSLAKQEILRSFLQSLGLSLSRGAELTSARLNRILEKVMNTQQQ
HHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHCCHHH
ELVNQVILRTQSQAEYNPQNIGHFGLNLHNYTHFTSPIRRYADLVIHRALIKALKLGNDQ
HHHHHHHHHCCCCCCCCCCCCCEECCEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
LTETQEKNLAEIATQISLYERRAMMAERETIDRLVAHYLTNKIGCTFTGRVSGVTKAGLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCEECCEE
ISLDTLNIDGFVHISTLKNDYYYFDEVQHTLVGKRSHKGYQLTDIVKVKLIKVQPFAGAL
EEEEEECCCCEEEEEEECCCCEEHHHHHHHHHCCCCCCCCEEEEEEEEEEEEECCCHHHH
CFELLTDPRPIAFSSASYHKNKFIAKKQKRYARNNIR
HHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AKGEKKTQYFQPLNLGRLPNKAEILSFINENPGRSSKREIAKAFNLKNDSRIWLRDILR
CCCCCCHHHHCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHH
DLKNQSLGAKERKKARNKGQLPAVAILKITARDEDGSFIAQPLEWEDGPKPKIEILPFHR
HHHCCCCCCHHHHHHHCCCCCCEEEEEEEEECCCCCCEEECCCCCCCCCCCCEEEEEEEC
TKGINVGVGDHILVKIFRNKVPQSLPYTGKIIRKIDVSPKSTFGIVRKLENGQWRLDSVE
CCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCEEEHHHH
RKTSELIIDTSSEIHVQSGDFVEVEIKKNTGYGLKSAQIKNVLGHIESEKTLSMIALLSK
HHCHHEEEECCCEEEEECCCEEEEEEECCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHC
GIPYIFPEDVLKQVKNVKPASIENREDWRQLPLITIDPPDAKDRDDAVYATKDKDPTNSG
CCCCCCCHHHHHHHHCCCCCCCCCCHHHHHCCEEEECCCCCCCCCCCEEEECCCCCCCCC
GWIIVVAIADVSYYVKTGSAIDKEALKRGNSVYFPDTVVPMLPERISNNLCSLHEGKEKP
CEEEEEEEECCEEEEECCCCCCHHHHHCCCCEECCCCHHHHCHHHHCCCHHHHCCCCCCC
ALAVRMIFDANGNKRKHSFHRVMVRIRANLSYQEVQSAIEGNINEKIAPLFENVLQPLWK
CEEEEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
AYECLKITRDRRQPLELEIAEKKIILDQNGCIKDVEIQTYLEAHRLIEELMIQANIAASE
HHHHHHHHHHCCCCCEEEEECEEEEECCCCCEECCHHHHHHHHHHHHHHHHHHHCCHHHH
TLKEHHQPLIYRIHDKPSLAKQEILRSFLQSLGLSLSRGAELTSARLNRILEKVMNTQQQ
HHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHCCHHH
ELVNQVILRTQSQAEYNPQNIGHFGLNLHNYTHFTSPIRRYADLVIHRALIKALKLGNDQ
HHHHHHHHHCCCCCCCCCCCCCEECCEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
LTETQEKNLAEIATQISLYERRAMMAERETIDRLVAHYLTNKIGCTFTGRVSGVTKAGLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCEECCEE
ISLDTLNIDGFVHISTLKNDYYYFDEVQHTLVGKRSHKGYQLTDIVKVKLIKVQPFAGAL
EEEEEECCCCEEEEEEECCCCEEHHHHHHHHHCCCCCCCCEEEEEEEEEEEEECCCHHHH
CFELLTDPRPIAFSSASYHKNKFIAKKQKRYARNNIR
HHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]