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The map label for this gene is lipB

Identifier: 49475555

GI number: 49475555

Start: 892916

End: 893653

Strand: Reverse

Name: lipB

Synonym: BH07850

Alternate gene names: 49475555

Gene position: 893653-892916 (Counterclockwise)

Preceding gene: 49475556

Following gene: 49475554

Centisome position: 46.28

GC content: 37.53

Gene sequence:

>738_bases
ATGACATTTGAATTGAAATATACTGACCGTAGTTATTTACCAGATCAGTTTAAAGCAATATCTGGAAATCCACCCGTTGA
ATGGAAAGTAACTAATAACTTGGTCGAATATCCCGAAGCGTTGCGTTATATGCAAGAGCGTGTAGAGAATATTTTGGCAC
AAAATGCACATGAACAAGTTTGGCTTCTTGAGCATCCTTCCCTTTATACAGCTGGTACAAGTGCGAAGAAAAAAGATCTT
TTAACACCTCATTTATTTCCTGTTTATGAGGCAGGACGTGGTGGTGAATTTACATATCATGGTCCAGGACAACGTATTGC
TTATATCATGCTTGATCTAAAACGCCGAAGACAAGACATTCGTGCTTTTATTAGTGCATTAGAAGAATGGATCATACAAA
TGCTTGCAAAATTTAATATTAAAGGTGAACGCCGCGAAGATCGCGTTGGGGTCTGGGTTCAAAGGCACAGTTGCCAATCA
ACACAAAACAGCCTTTATTCAGAAGATAAAATTGCAGCTATTGGTATTCGAGTGCGCAAATGGGTGAGTTTTCATGGCGT
TTCTATCAATGTTGATCCTAATTTAGCCCATTATTCAGGAATTGTTCCCTGTGGTATCACAAATCACGGTGTAACAAGCT
TTCTTGATCTAGGTTTACCTACAAAAATGCACGATATTGATATTGCTCTAAAACAATCCTTTGAACAAATTTTTGGACCA
ATAATTGATATTTCCTAA

Upstream 100 bases:

>100_bases
TTACCGTATTACCCAATTTTAATGAAGAATTTCATATCTTACTCTATAATTCTTTTACTGATTACAAACACAAACTTGAA
CACCAATATAAATATTACTT

Downstream 100 bases:

>100_bases
ATGCATTGATTTTTAAATAAAAAACAGGCATAAAATTCACTTTTATTGAGATTTTATTTTCATGAGGTATATAATCAAAC
GCCATGAGCGATAAAAACAA

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MTFELKYTDRSYLPDQFKAISGNPPVEWKVTNNLVEYPEALRYMQERVENILAQNAHEQVWLLEHPSLYTAGTSAKKKDL
LTPHLFPVYEAGRGGEFTYHGPGQRIAYIMLDLKRRRQDIRAFISALEEWIIQMLAKFNIKGERREDRVGVWVQRHSCQS
TQNSLYSEDKIAAIGIRVRKWVSFHGVSINVDPNLAHYSGIVPCGITNHGVTSFLDLGLPTKMHDIDIALKQSFEQIFGP
IIDIS

Sequences:

>Translated_245_residues
MTFELKYTDRSYLPDQFKAISGNPPVEWKVTNNLVEYPEALRYMQERVENILAQNAHEQVWLLEHPSLYTAGTSAKKKDL
LTPHLFPVYEAGRGGEFTYHGPGQRIAYIMLDLKRRRQDIRAFISALEEWIIQMLAKFNIKGERREDRVGVWVQRHSCQS
TQNSLYSEDKIAAIGIRVRKWVSFHGVSINVDPNLAHYSGIVPCGITNHGVTSFLDLGLPTKMHDIDIALKQSFEQIFGP
IIDIS
>Mature_244_residues
TFELKYTDRSYLPDQFKAISGNPPVEWKVTNNLVEYPEALRYMQERVENILAQNAHEQVWLLEHPSLYTAGTSAKKKDLL
TPHLFPVYEAGRGGEFTYHGPGQRIAYIMLDLKRRRQDIRAFISALEEWIIQMLAKFNIKGERREDRVGVWVQRHSCQST
QNSLYSEDKIAAIGIRVRKWVSFHGVSINVDPNLAHYSGIVPCGITNHGVTSFLDLGLPTKMHDIDIALKQSFEQIFGPI
IDIS

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Escherichia coli, GI87081767, Length=148, Percent_Identity=37.1621621621622, Blast_Score=125, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6323268, Length=197, Percent_Identity=27.4111675126904, Blast_Score=83, Evalue=4e-17,
Organism=Drosophila melanogaster, GI21355483, Length=185, Percent_Identity=31.8918918918919, Blast_Score=92, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_BARHE (Q6G3I3)

Other databases:

- EMBL:   BX897699
- RefSeq:   YP_033596.1
- ProteinModelPortal:   Q6G3I3
- SMR:   Q6G3I3
- GeneID:   2865585
- GenomeReviews:   BX897699_GR
- KEGG:   bhe:BH07850
- NMPDR:   fig|283166.1.peg.724
- HOGENOM:   HBG716160
- OMA:   DLKRRRP
- PhylomeDB:   Q6G3I3
- ProtClustDB:   PRK14341
- BioCyc:   BHEN283166:BH07850-MONOMER
- BRENDA:   2.3.1.181
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 28086; Mature: 27955

Theoretical pI: Translated: 7.75; Mature: 7.75

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 204-204

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFELKYTDRSYLPDQFKAISGNPPVEWKVTNNLVEYPEALRYMQERVENILAQNAHEQV
CEEEEEECCCCCCCHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCEE
WLLEHPSLYTAGTSAKKKDLLTPHLFPVYEAGRGGEFTYHGPGQRIAYIMLDLKRRRQDI
EEEECCCEEECCCCCCHHCCCCCCCCCCEECCCCCCEEEECCCCEEHHHHHHHHHHHHHH
RAFISALEEWIIQMLAKFNIKGERREDRVGVWVQRHSCQSTQNSLYSEDKIAAIGIRVRK
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHEEEEEHHHHHHHHHHHCCCCCEEEHHHHHHH
WVSFHGVSINVDPNLAHYSGIVPCGITNHGVTSFLDLGLPTKMHDIDIALKQSFEQIFGP
HHEECCEEEEECCCHHHHCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHCCH
IIDIS
HCCCC
>Mature Secondary Structure 
TFELKYTDRSYLPDQFKAISGNPPVEWKVTNNLVEYPEALRYMQERVENILAQNAHEQV
EEEEEECCCCCCCHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCEE
WLLEHPSLYTAGTSAKKKDLLTPHLFPVYEAGRGGEFTYHGPGQRIAYIMLDLKRRRQDI
EEEECCCEEECCCCCCHHCCCCCCCCCCEECCCCCCEEEECCCCEEHHHHHHHHHHHHHH
RAFISALEEWIIQMLAKFNIKGERREDRVGVWVQRHSCQSTQNSLYSEDKIAAIGIRVRK
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHEEEEEHHHHHHHHHHHCCCCCEEEHHHHHHH
WVSFHGVSINVDPNLAHYSGIVPCGITNHGVTSFLDLGLPTKMHDIDIALKQSFEQIFGP
HHEECCEEEEECCCHHHHCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHCCH
IIDIS
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA