Click here to switch to the map view.

The map label for this gene is parE [H]

Identifier: 49475554

GI number: 49475554

Start: 890775

End: 892832

Strand: Reverse

Name: parE [H]

Synonym: BH07840

Alternate gene names: 49475554

Gene position: 892832-890775 (Counterclockwise)

Preceding gene: 49475555

Following gene: 49475552

Centisome position: 46.24

GC content: 40.77

Gene sequence:

>2058_bases
ATGAGCGATAAAAACAAAGATCTTTTTAGTATTTTAAATAATGCTCAGTTTAGCATAAATATAAAAGAAAAAAATCTTTC
CCCCTCGCTGAGTTCAGAAGCAGCGAATTCGAGAAAAGATACGCAAGAAAACGACTATAACGCGCTATCTATTCGTGTGC
TTGAGGGGCTAGAGCCCGTGCGTTTACGTCCTGGAATGTATATTGGTGGGACAGACAGCAAAGCACTTCATCATTTATTT
GCCGAAATCATTGACAATGCAATGGATGAAGCAGTTGCTGGTTATGCGGACTTCATTGATGTGTCCTTAGAGAAAAACGG
CTATTTAACAGTTACAGATAATGGACGCGGTATTCCTGTCGAAAATCATCCTCAAATGCCGGGAAAATCAACTCTTGAAG
TCATTATGACACAACTCCATTCAGGTGGTAAATTTGATGGCAAAGCTTATCAAACAGCTGGTGGACTTCATGGTGTAGGA
ATTTCTGTTGTCAATGCTCTCTCTGACTATATGGAAGTTGAAGTAGCACGAGAACGCAAACTTTACCGACAACGCTTCTC
GCGCGGTATTCCTCAATCTGGATTGGAAGATTTGGGTGATGTCTATAATCGTCGCGGCACGCGTGTTCGTTTTCACCCTG
ATAGTAAAATTTTTGGTGAAAAAGCAGCTTTCGATCCAGAAAAAATTTATAAAATGGCACGCTCCAAGGCTTATCTTTTT
GGTGGTGTAAAAATTCGTTGGAATTGTGATCCTGTCTTACTTGAAAATACAAAAAATATTCCCGAAAAAGCTGTTTTTCA
TTTCCCAGATGGACTTAAAGATTATTTACTCTCATCATTGAAAGATGAATATCGGGTAACATCAGAAATTTTTTCTGGCA
AAACAAAACACTCTAATGGTCATGGCTCTGTTGAATGGGCTATCGCCTGGCATGGTGGCGATTCCCATGTACAGTCTTAC
TGCAATACTATTCCCACCGGAGAAGGTGGAACACACGAAATGGGACTGCGTCAAGCTCTTTTGCGCGGATTGAAATCTTA
CGCTGAATTAATAGGAAATAAGCGCGCTGCTATCATTACGTCTGATGACGTTATGATTTCAACTTCTGCAATACTTTCAG
TGTTTATTAAAGATCCCGAATTTGTCGGACAAACCAAAGATCGATTAGCGACAATCGAAGCTCAGCGTATCGTTGAGAAT
GCAATACGCGATCCTTTCGATCATTGGCTGACAAATTCTCCACAAGAATCAACAAAACTTCTTGATTGGGTTGTTGAACG
TGCTGAGGAACGCGTTAGACGGCGCCAAGACAGAGAAATAAATCGAAAAACAGCTGTACGTAAATTACGTCTCCCCGGTA
AACTAGCAGATTGTAGCCAAAACTGTGCTGCTGGTGCTGAATTATTCATTGTTGAAGGTGACTCTGCTGGTGGTTCGGCT
AAACAAGCGCGCAATAGAGCAAACCAAGCAATTTTACCTCTTCGTGGAAAAATTTTAAATGTAGCCAGTGCTGCACACGA
AAAAATGAACTCAAGCCAAACAATTGGTGATCTTATACTTGCTCTTGGATGTGGAACGCGCTCCAAATATCGCGAAAAAG
ATCTCAGATATGAACGTATTATCATTATGACAGATGCAGATGTTGATGGCGCTCATATTGCCTCACTGCTCATCACCTTC
TTCTTTCAAGAGATGCCCGATCTTATTCGTCAGGGACATTTATACCTTGCTGTGCCTCCCCTTTACAGAATATCGCAAGG
AGGGAAAGTCGCTTACGCACGTGATGACACTCATAAAGATGAATTATTAAAAACTGAATTCACTGGAAAAGCTAAAATTG
AAATTGGACGTTTCAAAGGACTTGGTGAAATGCGTGCTGAACAGCTTAAAGAAACCACCATGCATCCTCAAAAACGTACA
CTGCTACGCGTCTCTATTAATGCAGAAGAAGTGCAAGAAACCAAAAACACTGTAGAAAGCTTAATGGGAACAAAACCAGA
AGCACGGTTTCGATTCATACAGGAAAACTCTACTTTTGCTTCAAATCTAGATATCTGA

Upstream 100 bases:

>100_bases
TAATTGATATTTCCTAAATGCATTGATTTTTAAATAAAAAACAGGCATAAAATTCACTTTTATTGAGATTTTATTTTCAT
GAGGTATATAATCAAACGCC

Downstream 100 bases:

>100_bases
TAATTTCAGAAAATATTCTTCTCATATCACTGCAGCTGCATCAATAACGCGAAGTTGTGGACTGATATTTCCATTCCAAT
AATTTAAACTAATATTACCG

Product: DNA topoisomerase IV subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 685; Mature: 684

Protein sequence:

>685_residues
MSDKNKDLFSILNNAQFSINIKEKNLSPSLSSEAANSRKDTQENDYNALSIRVLEGLEPVRLRPGMYIGGTDSKALHHLF
AEIIDNAMDEAVAGYADFIDVSLEKNGYLTVTDNGRGIPVENHPQMPGKSTLEVIMTQLHSGGKFDGKAYQTAGGLHGVG
ISVVNALSDYMEVEVARERKLYRQRFSRGIPQSGLEDLGDVYNRRGTRVRFHPDSKIFGEKAAFDPEKIYKMARSKAYLF
GGVKIRWNCDPVLLENTKNIPEKAVFHFPDGLKDYLLSSLKDEYRVTSEIFSGKTKHSNGHGSVEWAIAWHGGDSHVQSY
CNTIPTGEGGTHEMGLRQALLRGLKSYAELIGNKRAAIITSDDVMISTSAILSVFIKDPEFVGQTKDRLATIEAQRIVEN
AIRDPFDHWLTNSPQESTKLLDWVVERAEERVRRRQDREINRKTAVRKLRLPGKLADCSQNCAAGAELFIVEGDSAGGSA
KQARNRANQAILPLRGKILNVASAAHEKMNSSQTIGDLILALGCGTRSKYREKDLRYERIIIMTDADVDGAHIASLLITF
FFQEMPDLIRQGHLYLAVPPLYRISQGGKVAYARDDTHKDELLKTEFTGKAKIEIGRFKGLGEMRAEQLKETTMHPQKRT
LLRVSINAEEVQETKNTVESLMGTKPEARFRFIQENSTFASNLDI

Sequences:

>Translated_685_residues
MSDKNKDLFSILNNAQFSINIKEKNLSPSLSSEAANSRKDTQENDYNALSIRVLEGLEPVRLRPGMYIGGTDSKALHHLF
AEIIDNAMDEAVAGYADFIDVSLEKNGYLTVTDNGRGIPVENHPQMPGKSTLEVIMTQLHSGGKFDGKAYQTAGGLHGVG
ISVVNALSDYMEVEVARERKLYRQRFSRGIPQSGLEDLGDVYNRRGTRVRFHPDSKIFGEKAAFDPEKIYKMARSKAYLF
GGVKIRWNCDPVLLENTKNIPEKAVFHFPDGLKDYLLSSLKDEYRVTSEIFSGKTKHSNGHGSVEWAIAWHGGDSHVQSY
CNTIPTGEGGTHEMGLRQALLRGLKSYAELIGNKRAAIITSDDVMISTSAILSVFIKDPEFVGQTKDRLATIEAQRIVEN
AIRDPFDHWLTNSPQESTKLLDWVVERAEERVRRRQDREINRKTAVRKLRLPGKLADCSQNCAAGAELFIVEGDSAGGSA
KQARNRANQAILPLRGKILNVASAAHEKMNSSQTIGDLILALGCGTRSKYREKDLRYERIIIMTDADVDGAHIASLLITF
FFQEMPDLIRQGHLYLAVPPLYRISQGGKVAYARDDTHKDELLKTEFTGKAKIEIGRFKGLGEMRAEQLKETTMHPQKRT
LLRVSINAEEVQETKNTVESLMGTKPEARFRFIQENSTFASNLDI
>Mature_684_residues
SDKNKDLFSILNNAQFSINIKEKNLSPSLSSEAANSRKDTQENDYNALSIRVLEGLEPVRLRPGMYIGGTDSKALHHLFA
EIIDNAMDEAVAGYADFIDVSLEKNGYLTVTDNGRGIPVENHPQMPGKSTLEVIMTQLHSGGKFDGKAYQTAGGLHGVGI
SVVNALSDYMEVEVARERKLYRQRFSRGIPQSGLEDLGDVYNRRGTRVRFHPDSKIFGEKAAFDPEKIYKMARSKAYLFG
GVKIRWNCDPVLLENTKNIPEKAVFHFPDGLKDYLLSSLKDEYRVTSEIFSGKTKHSNGHGSVEWAIAWHGGDSHVQSYC
NTIPTGEGGTHEMGLRQALLRGLKSYAELIGNKRAAIITSDDVMISTSAILSVFIKDPEFVGQTKDRLATIEAQRIVENA
IRDPFDHWLTNSPQESTKLLDWVVERAEERVRRRQDREINRKTAVRKLRLPGKLADCSQNCAAGAELFIVEGDSAGGSAK
QARNRANQAILPLRGKILNVASAAHEKMNSSQTIGDLILALGCGTRSKYREKDLRYERIIIMTDADVDGAHIASLLITFF
FQEMPDLIRQGHLYLAVPPLYRISQGGKVAYARDDTHKDELLKTEFTGKAKIEIGRFKGLGEMRAEQLKETTMHPQKRTL
LRVSINAEEVQETKNTVESLMGTKPEARFRFIQENSTFASNLDI

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=621, Percent_Identity=25.9259259259259, Blast_Score=148, Evalue=2e-35,
Organism=Homo sapiens, GI19913408, Length=619, Percent_Identity=25.3634894991922, Blast_Score=132, Evalue=9e-31,
Organism=Escherichia coli, GI1789408, Length=640, Percent_Identity=40.15625, Blast_Score=439, Evalue=1e-124,
Organism=Escherichia coli, GI48994957, Length=564, Percent_Identity=43.6170212765958, Blast_Score=435, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI212645845, Length=571, Percent_Identity=23.1173380035026, Blast_Score=111, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17535065, Length=668, Percent_Identity=22.6047904191617, Blast_Score=110, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6324241, Length=640, Percent_Identity=25.625, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI17136538, Length=641, Percent_Identity=24.3369734789392, Blast_Score=122, Evalue=6e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR005737 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 76508; Mature: 76377

Theoretical pI: Translated: 8.41; Mature: 8.41

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDKNKDLFSILNNAQFSINIKEKNLSPSLSSEAANSRKDTQENDYNALSIRVLEGLEPV
CCCCCHHHHHHHCCCEEEEEEECCCCCCCHHHHHHCCCCCCCCCCCCEEEEEEECCCCCE
RLRPGMYIGGTDSKALHHLFAEIIDNAMDEAVAGYADFIDVSLEKNGYLTVTDNGRGIPV
EECCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCHHHHEEEEECCCCEEEECCCCCCCCC
ENHPQMPGKSTLEVIMTQLHSGGKFDGKAYQTAGGLHGVGISVVNALSDYMEVEVARERK
CCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LYRQRFSRGIPQSGLEDLGDVYNRRGTRVRFHPDSKIFGEKAAFDPEKIYKMARSKAYLF
HHHHHHHCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCEEEE
GGVKIRWNCDPVLLENTKNIPEKAVFHFPDGLKDYLLSSLKDEYRVTSEIFSGKTKHSNG
ECEEEEECCCEEEECCCCCCCHHHEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
HGSVEWAIAWHGGDSHVQSYCNTIPTGEGGTHEMGLRQALLRGLKSYAELIGNKRAAIIT
CCCEEEEEEEECCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
SDDVMISTSAILSVFIKDPEFVGQTKDRLATIEAQRIVENAIRDPFDHWLTNSPQESTKL
CCCEEEEHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHH
LDWVVERAEERVRRRQDREINRKTAVRKLRLPGKLADCSQNCAAGAELFIVEGDSAGGSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCEEEEEECCCCCCCH
KQARNRANQAILPLRGKILNVASAAHEKMNSSQTIGDLILALGCGTRSKYREKDLRYERI
HHHHHHHCCCEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHCCCEEEE
IIMTDADVDGAHIASLLITFFFQEMPDLIRQGHLYLAVPPLYRISQGGKVAYARDDTHKD
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHEECCCCEEEEECCCCCHH
ELLKTEFTGKAKIEIGRFKGLGEMRAEQLKETTMHPQKRTLLRVSINAEEVQETKNTVES
HHHHHCCCCCEEEEEHHHCCCHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHH
LMGTKPEARFRFIQENSTFASNLDI
HHCCCCCHHEEEEECCCCCCCCCCC
>Mature Secondary Structure 
SDKNKDLFSILNNAQFSINIKEKNLSPSLSSEAANSRKDTQENDYNALSIRVLEGLEPV
CCCCHHHHHHHCCCEEEEEEECCCCCCCHHHHHHCCCCCCCCCCCCEEEEEEECCCCCE
RLRPGMYIGGTDSKALHHLFAEIIDNAMDEAVAGYADFIDVSLEKNGYLTVTDNGRGIPV
EECCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCHHHHEEEEECCCCEEEECCCCCCCCC
ENHPQMPGKSTLEVIMTQLHSGGKFDGKAYQTAGGLHGVGISVVNALSDYMEVEVARERK
CCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LYRQRFSRGIPQSGLEDLGDVYNRRGTRVRFHPDSKIFGEKAAFDPEKIYKMARSKAYLF
HHHHHHHCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCEEEE
GGVKIRWNCDPVLLENTKNIPEKAVFHFPDGLKDYLLSSLKDEYRVTSEIFSGKTKHSNG
ECEEEEECCCEEEECCCCCCCHHHEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
HGSVEWAIAWHGGDSHVQSYCNTIPTGEGGTHEMGLRQALLRGLKSYAELIGNKRAAIIT
CCCEEEEEEEECCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
SDDVMISTSAILSVFIKDPEFVGQTKDRLATIEAQRIVENAIRDPFDHWLTNSPQESTKL
CCCEEEEHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHH
LDWVVERAEERVRRRQDREINRKTAVRKLRLPGKLADCSQNCAAGAELFIVEGDSAGGSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCEEEEEECCCCCCCH
KQARNRANQAILPLRGKILNVASAAHEKMNSSQTIGDLILALGCGTRSKYREKDLRYERI
HHHHHHHCCCEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHCCCEEEE
IIMTDADVDGAHIASLLITFFFQEMPDLIRQGHLYLAVPPLYRISQGGKVAYARDDTHKD
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHEECCCCEEEEECCCCCHH
ELLKTEFTGKAKIEIGRFKGLGEMRAEQLKETTMHPQKRTLLRVSINAEEVQETKNTVES
HHHHHCCCCCEEEEEHHHCCCHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHH
LMGTKPEARFRFIQENSTFASNLDI
HHCCCCCHHEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9797224 [H]