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The map label for this gene is rlmB [H]

Identifier: 49475390

GI number: 49475390

Start: 703135

End: 703959

Strand: Reverse

Name: rlmB [H]

Synonym: BH05990

Alternate gene names: 49475390

Gene position: 703959-703135 (Counterclockwise)

Preceding gene: 49475403

Following gene: 49475384

Centisome position: 36.45

GC content: 39.15

Gene sequence:

>825_bases
ATGAGAGAAAAAATATCGAAAAGTTTTCACTATAATCGCTTACAGCGTCGATCTCACGATAAAAAAGGCTTTCTACCTGC
AGCGCGCATGAAACAGAAATCTGTTCCCATGAGACATGGAACAATCTATCTTTATGGTCTTCATTCCGTTCATGCAGCTT
TAAAAAATCCTAAAAGAGTTTTTGAACATCTCTATATCACACCAAATGCCTTAAAACGATTAAATATACCCGAATCAGAT
TTACCTTGTCCCATCACATTATGCTTACCCAAACAACTTGATTCGTTCGTTGGAAATGATGCAGTTCATCAAGGTATTGT
TTTGGAAACTGAACCTCTCAAGCCGTGTCAATTATCCGAACTAACAAATACTGATCTTGTCATTGTAATGGATCAAATTA
CTGATCCACATAATGTCGGCGCTATTATGCGCTCTGCAGTCGCATTTAAAGCTGAGGCACTTATTACAACTTATCGCCAC
TCCCCCCAAGAAAGCGGTGTTCTTGCTAAAGCCGCATCCGGAGCGTTGGAAATGATTCATTATATCACCGTACAAAACCT
CGCAGAAACACTCCAAGAACTCCATAAAGCAGGCTTTATCAGTTTTGGACTTGATTCAGAAGGCAATCATCCTCTCGAAA
CAACATTAACAGGAGAAAAAATTGCTCTTGTTCTAGGAGCAGAAGGTAAAGGCTTACGCAAAAAAACACGTGAAAACGTT
TGTGCATTAACACGCCTTGATATGCCTGGAAATATTAAATCGTTAAACGTTTCAAACGCAGCAACAATAGCGCTCTATGC
AGCTCATAAATATCTTAGAGGATAA

Upstream 100 bases:

>100_bases
TCAACAGAATAAATACGATTCCGTACAAATTTCTTGCTAATAATTTTAAACCACAGCTTTTTTCTCTCTACTTTTATTAT
TAACGCGCTATAAAGAGGAT

Downstream 100 bases:

>100_bases
TCTTTACTTTACCGGGCGAAACGTCAAAAATACCAACCAACAAAAAACCCGAAACAAGCCCACCAATGTGGGCCTCCCAT
GCAATTGAAATATCGTCTCT

Product: tRNA /rRNA methyltransferase

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MREKISKSFHYNRLQRRSHDKKGFLPAARMKQKSVPMRHGTIYLYGLHSVHAALKNPKRVFEHLYITPNALKRLNIPESD
LPCPITLCLPKQLDSFVGNDAVHQGIVLETEPLKPCQLSELTNTDLVIVMDQITDPHNVGAIMRSAVAFKAEALITTYRH
SPQESGVLAKAASGALEMIHYITVQNLAETLQELHKAGFISFGLDSEGNHPLETTLTGEKIALVLGAEGKGLRKKTRENV
CALTRLDMPGNIKSLNVSNAATIALYAAHKYLRG

Sequences:

>Translated_274_residues
MREKISKSFHYNRLQRRSHDKKGFLPAARMKQKSVPMRHGTIYLYGLHSVHAALKNPKRVFEHLYITPNALKRLNIPESD
LPCPITLCLPKQLDSFVGNDAVHQGIVLETEPLKPCQLSELTNTDLVIVMDQITDPHNVGAIMRSAVAFKAEALITTYRH
SPQESGVLAKAASGALEMIHYITVQNLAETLQELHKAGFISFGLDSEGNHPLETTLTGEKIALVLGAEGKGLRKKTRENV
CALTRLDMPGNIKSLNVSNAATIALYAAHKYLRG
>Mature_274_residues
MREKISKSFHYNRLQRRSHDKKGFLPAARMKQKSVPMRHGTIYLYGLHSVHAALKNPKRVFEHLYITPNALKRLNIPESD
LPCPITLCLPKQLDSFVGNDAVHQGIVLETEPLKPCQLSELTNTDLVIVMDQITDPHNVGAIMRSAVAFKAEALITTYRH
SPQESGVLAKAASGALEMIHYITVQNLAETLQELHKAGFISFGLDSEGNHPLETTLTGEKIALVLGAEGKGLRKKTRENV
CALTRLDMPGNIKSLNVSNAATIALYAAHKYLRG

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=195, Percent_Identity=30.2564102564103, Blast_Score=82, Evalue=5e-16,
Organism=Escherichia coli, GI1790623, Length=237, Percent_Identity=34.5991561181435, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1788935, Length=148, Percent_Identity=30.4054054054054, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 30320; Mature: 30320

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREKISKSFHYNRLQRRSHDKKGFLPAARMKQKSVPMRHGTIYLYGLHSVHAALKNPKRV
CCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCEEEEEEHHHHHHHHCCHHHH
FEHLYITPNALKRLNIPESDLPCPITLCLPKQLDSFVGNDAVHQGIVLETEPLKPCQLSE
HHEEECCHHHHHHCCCCCCCCCCCEEEECHHHHHHHHCCHHHHCCEEEECCCCCCCCHHH
LTNTDLVIVMDQITDPHNVGAIMRSAVAFKAEALITTYRHSPQESGVLAKAASGALEMIH
CCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
YITVQNLAETLQELHKAGFISFGLDSEGNHPLETTLTGEKIALVLGAEGKGLRKKTRENV
HHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCEEEECCCEEEEEECCCCCCHHHHHHHHH
CALTRLDMPGNIKSLNVSNAATIALYAAHKYLRG
EEEEEECCCCCCEEEECCCCCEEHHHHHHHHHCC
>Mature Secondary Structure
MREKISKSFHYNRLQRRSHDKKGFLPAARMKQKSVPMRHGTIYLYGLHSVHAALKNPKRV
CCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCEEEEEEHHHHHHHHCCHHHH
FEHLYITPNALKRLNIPESDLPCPITLCLPKQLDSFVGNDAVHQGIVLETEPLKPCQLSE
HHEEECCHHHHHHCCCCCCCCCCCEEEECHHHHHHHHCCHHHHCCEEEECCCCCCCCHHH
LTNTDLVIVMDQITDPHNVGAIMRSAVAFKAEALITTYRHSPQESGVLAKAASGALEMIH
CCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
YITVQNLAETLQELHKAGFISFGLDSEGNHPLETTLTGEKIALVLGAEGKGLRKKTRENV
HHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCEEEECCCEEEEEECCCCCCHHHHHHHHH
CALTRLDMPGNIKSLNVSNAATIALYAAHKYLRG
EEEEEECCCCCCEEEECCCCCEEHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]